Restriction map for
pFN21AE6429
(Circular) (Six-base) MAPSORT of: pFN21AE6429.seq Check: 192 from: 1 to: 6490
pFN21AE6429 6490 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2716 2716
Size: 6490
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 3833 1937
Size: 939 957 4594
Fragments arranged by size:
4594 957 939
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4553 5937 6205 1632
Size: 2921 1384 268 1917
Fragments arranged by size:
2921 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 2318 5389 6366 1203
Size: 1115 3071 977 1327
Fragments arranged by size:
3071 1327 1115 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 2872 5219 2872
Size: 2347 4143
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 2043 4233 5805 2043
Size: 2190 1572 2728
Fragments arranged by size:
2728 2190 1572
ApaI G_GGCC'C
Cuts at: 2178 2178
Size: 6490
ApaLI G'TGCA_C
Cuts at: 4547 5703 4547
Size: 1156 5334
ApoI r'AATT_y
Cuts at: 1493 2260 3799 4025 4177 6286 1493
Size: 767 1539 226 152 2109 1697
Fragments arranged by size:
2109 1697 1539 767 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 3815 1870
Size: 73 68 1804 4545
Fragments arranged by size:
4545 1804 73 68
AvrII C'CTAG_G
Cuts at: 3676 3676
Size: 6490
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2547 3811 5266 618
Size: 325 217 627 760 1264 1455 1842
Fragments arranged by size:
1842 1455 1264 760 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2178 3809 1391
Size: 621 166 1631 4072
Fragments arranged by size:
4072 1631 621 166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2898 3759 928
Size: 971 999 861 3659
Fragments arranged by size:
3659 999 971 861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 2539 1548
Size: 991 5499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5101 136
Size: 107 122 71 631 605 3429 1525
Fragments arranged by size:
3429 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3426 6485 3426
Size: 3059 3431
BlpI GC'TnA_GC
Cuts at: 3907 3907
Size: 6490
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2326 4551 5707 1790
Size: 388 148 2225 1156 2573
Fragments arranged by size:
2573 2225 1156 388 148
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4221 5179 442
Size: 416 53 83 399 24 2804 958 1753
Fragments arranged by size:
2804 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 5150 1239
Size: 876 3035 2579
Fragments arranged by size:
3035 2579 876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3169 3424 4615 5480 5778 3101
Size: 68 255 1191 865 298 3813
Fragments arranged by size:
3813 1191 865 298 255 68
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5153 882
Size: 617 3654 2219
Fragments arranged by size:
3654 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4281 493
Size: 711 3077 2702
Fragments arranged by size:
3077 2702 711
BsaBI GATnn'nnATC
Cuts at: 2817 6484 2817
Size: 3667 2823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4679 5314 275
Size: 53 83 186 936 2764 382 635 1451
Fragments arranged by size:
2764 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4923 5321 5595 5742 6153 4923
Size: 398 274 147 411 5260
Fragments arranged by size:
5260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3602 3632 1178
Size: 30 165 30 2199 30 4036
Fragments arranged by size:
4036 2199 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2239 3038 1186
Size: 1053 799 4638
Fragments arranged by size:
4638 1053 799
BseYI C'CCAG_C
Cuts at: 2052 2085 2121 5693 6189 2052
Size: 33 36 3572 496 2353
Fragments arranged by size:
3572 2353 496 36 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 2592 1163
Size: 573 477 379 5061
Fragments arranged by size:
5061 573 477 379
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2229 4551 4636 5707 2012
Size: 217 2322 85 1071 2795
Fragments arranged by size:
2795 2322 1071 217 85
BsmI GAATG_Cn'
Cuts at: 3321 4001 4094 3321
Size: 680 93 5717
Fragments arranged by size:
5717 680 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2021 2021
Size: 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2178 2229 2326 3809 4551 4636
Size: 399 222 166 51 97 1483 742 85
Cuts at: 4636 5707 1391
Size: 1071 2174
Fragments arranged by size:
2174 1483 1071 742 399 222 166 97
85 51
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 3514 4379 6109 3514
Size: 865 1730 3895
Fragments arranged by size:
3895 1730 865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2716 3310 3845 844
Size: 1872 594 535 3489
Fragments arranged by size:
3489 1872 594 535
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4985 5159 65
Size: 1183 3737 174 1396
Fragments arranged by size:
3737 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5134 5321 1700
Size: 110 3324 187 2869
Fragments arranged by size:
3324 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6490
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 2812 3426 3820 4187 4572 4589 6041 6127
Size: 840 614 394 367 385 17 1452 86
Cuts at: 6127 6139 6485 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 840 614 394 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2556 2556
Size: 6490
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5751
Fragments arranged by size:
5751 683 56
BtsI GCAGTG_nn'
Cuts at: 2081 2095 2821 4006 4800 4820 2081
Size: 14 726 1185 794 20 3751
Fragments arranged by size:
3751 1185 794 726 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 2307 3794 2307
Size: 1487 5003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3937 4826 6455
Size: 54 1425 50 275 18 2107 889 1629
Cuts at: 6455 8
Size: 43
Fragments arranged by size:
2107 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3231 3531 4420 1353
Size: 1878 300 889 3423
Fragments arranged by size:
3423 1878 889 300
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5077 5451 5597 387
Size: 1064 138 3488 374 146 1280
Fragments arranged by size:
3488 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 6490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 4553 5150 5937 6205 1239
Size: 393 483 2438 597 787 268 1524
Fragments arranged by size:
2438 1524 787 597 483 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2163 3441 2163
Size: 1278 5212
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 5637 1678
Size: 3959 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2858 2885 2890 2917 3038
Size: 32 135 32 1286 27 5 27 121
Cuts at: 3038 3070 3652 3684 5131 5163 5205 5237 1373
Size: 32 582 32 1447 32 42 32 2626
Fragments arranged by size:
2626 1447 1286 582 135 121 42 32
32 32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3509 3526 748
Size: 2761 17 3712
Fragments arranged by size:
3712 2761 17
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2324 2399 2608 2868 3013 5604 5788 1305
Size: 1019 75 209 260 145 2591 184 2007
Fragments arranged by size:
2591 2007 1019 260 209 184 145 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4668
Fragments arranged by size:
4668 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 3465 4448 4807 4966 518
Size: 648 2223 76 983 359 159 2042
Fragments arranged by size:
2223 2042 983 648 359 159 76
MspA1I CmG'CkG
Cuts at: 1606 2043 3906 4583 5731 5976 1606
Size: 437 1863 677 1148 245 2120
Fragments arranged by size:
2120 1863 1148 677 437 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5938
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 2322 3848 5393 1132
Size: 97 1093 1526 1545 2229
Fragments arranged by size:
2229 1545 1526 1093 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 2318 5389 2318
Size: 3071 3419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 3205 1108
Size: 1155 942 4393
Fragments arranged by size:
4393 1155 942
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 6072 6104 4559
Size: 32 1481 32 4945
Fragments arranged by size:
4945 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2163 3441 2163
Size: 1278 5212
PshAI GACnn'nnGTC
Cuts at: 1613 3439 1613
Size: 1826 4664
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PspOMI G'GGCC_C
Cuts at: 2174 2174
Size: 6490
PspXI vC'TCGA_Gb
Cuts at: 2011 2011
Size: 6490
PstI C_TGCA'G
Cuts at: 830 2076 2961 3434 3842 830
Size: 1246 885 473 408 3478
Fragments arranged by size:
3478 1246 885 473 408
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2043 2043
Size: 6490
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 2332 4738 6450 2332
Size: 2406 1712 2372
Fragments arranged by size:
2406 2372 1712
SexAI A'CCwGG_T
Cuts at: 3712 3712
Size: 6490
SfcI C'TryA_G
Cuts at: 826 1045 2072 2167 2877 2957 3430 3838 4973
Size: 219 1027 95 710 80 473 408 1135
Cuts at: 4973 5654 5845 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1027 710 681 473 408 219
191 95 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2011 3080 3184 3403 4594 5495
Size: 197 926 68 1069 104 219 1191 901
Cuts at: 5495 5757 820
Size: 262 1553
Fragments arranged by size:
1553 1191 1069 926 901 262 219 197
104 68
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 2992 152
Size: 2840 3650
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 3275 4414 5
Size: 47 3223 1139 2081
Fragments arranged by size:
3223 2081 1139 47
StuI AGG'CCT
Cuts at: 1517 2198 3681 1517
Size: 681 1483 4326
Fragments arranged by size:
4326 1483 681
StyI C'CwwG_G
Cuts at: 513 1065 2199 3676 3929 513
Size: 552 1134 1477 253 3074
Fragments arranged by size:
3074 1477 1134 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4517 4534 4687 4872 5317 325
Size: 1255 2937 17 153 185 445 1498
Fragments arranged by size:
2937 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2330 2425 3654
Size: 274 80 33 51 1443 353 95 1229
Cuts at: 3654 4736 6448 96
Size: 1082 1712 138
Fragments arranged by size:
1712 1443 1229 1082 353 274 138 95
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2155 2187 6450 6482 2155
Size: 32 4263 32 2163
Fragments arranged by size:
4263 2163 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 3620 2185
Size: 1435 5055
XhoI C'TCGA_G
Cuts at: 1943 2011 1943
Size: 68 6422
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 4619 4619
Size: 6490
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI