Restriction map for
pFN21AE6423
(Circular) (Six-base) MAPSORT of: pFN21AE6423.seq Check: 2588 from: 1 to: 6100
pFN21AE6423 6100 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2227 2227
Size: 6100
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2322 3222 3443 1937
Size: 385 900 221 4594
Fragments arranged by size:
4594 900 385 221
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2480 2518 2876 4163 5547 5815 1632
Size: 662 186 38 358 1287 1384 268 1917
Fragments arranged by size:
1917 1384 1287 662 358 268 186 38
AfeI AGC'GCT
Cuts at: 2306 2465 2306
Size: 159 5941
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 6068
AlwNI CAG_nnn'CTG
Cuts at: 2215 2866 3843 5415 2215
Size: 651 977 1572 2900
Fragments arranged by size:
2900 1572 977 651
ApaI G_GGCC'C
Cuts at: 3040 3040
Size: 6100
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 2591 2885 3409 3635 3787 5896 1493
Size: 1098 294 524 226 152 2109 1697
Fragments arranged by size:
2109 1697 1098 524 294 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 3425 1870
Size: 73 1482 4545
Fragments arranged by size:
4545 1482 73
BamHI G'GATC_C
Cuts at: 2083 3430 3797 2083
Size: 1347 367 4386
Fragments arranged by size:
4386 1347 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2389 3421 4876 618
Size: 325 217 627 602 1032 1455 1842
Fragments arranged by size:
1842 1455 1032 627 602 325 217
BanII G_rGCy'C
Cuts at: 1391 2127 2247 2458 3040 3419 1391
Size: 736 120 211 582 379 4072
Fragments arranged by size:
4072 736 582 379 211 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2341 928
Size: 971 442 4687
Fragments arranged by size:
4687 971 442
BbvCI CC'TCA_GC
Cuts at: 2058 2058
Size: 6100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2794 2828 2891 2925 3148 3182 4291
Size: 34 857 34 63 34 223 34 1109
Cuts at: 4291 4325 1903
Size: 34 3678
Fragments arranged by size:
3678 1109 857 223 63 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2906 4711 136
Size: 107 122 71 631 605 1234 1805 1525
Fragments arranged by size:
1805 1525 1234 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2098 2758 6095 2098
Size: 660 3337 2103
Fragments arranged by size:
3337 2103 660
BlpI GC'TnA_GC
Cuts at: 3323 3517 3323
Size: 194 5906
Bme1580I G_kGCm'C
Cuts at: 1790 3040 3282 4161 5317 1790
Size: 1250 242 879 1156 2573
Fragments arranged by size:
2573 1250 1156 879 242
BmgBI CAC'GTC
Cuts at: 1318 2941 3220 1318
Size: 1623 279 4198
Fragments arranged by size:
4198 1623 279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2151 3831 4789
Size: 416 53 83 399 24 734 1680 958
Cuts at: 4789 442
Size: 1753
Fragments arranged by size:
1753 1680 958 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3140 4760 1239
Size: 1901 1620 2579
Fragments arranged by size:
2579 1901 1620
Bpu10I CC'TnA_GC
Cuts at: 2058 2058
Size: 6100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 4225 5090 5388 2501
Size: 1724 865 298 3213
Fragments arranged by size:
3213 1724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4763 882
Size: 617 3264 2219
Fragments arranged by size:
3264 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2844 3891 493
Size: 711 1640 1047 2702
Fragments arranged by size:
2702 1640 1047 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2093 3907 4289 4924
Size: 53 83 186 936 560 1814 382 635
Cuts at: 4924 275
Size: 1451
Fragments arranged by size:
1814 1451 936 635 560 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2251 2679 4533 4931 5205 5352 5763 2251
Size: 428 1854 398 274 147 411 2588
Fragments arranged by size:
2588 1854 428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3164 3194 1178
Size: 30 165 30 1761 30 4084
Fragments arranged by size:
4084 1761 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2464 1186
Size: 904 374 4822
Fragments arranged by size:
4822 904 374
BseYI C'CCAG_C
Cuts at: 2149 2416 2635 5303 5799 2149
Size: 267 219 2668 496 2450
Fragments arranged by size:
2668 2450 496 267 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2255 2351 2570 2895 3026 3179
Size: 573 423 96 96 219 325 131 153
Cuts at: 3179 1163
Size: 4084
Fragments arranged by size:
4084 573 423 325 219 153 131 96
96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 3371 4161 4246 5317 2247
Size: 1124 790 85 1071 3030
Fragments arranged by size:
3030 1124 1071 790 85
BsmI GAATG_Cn'
Cuts at: 2169 2418 3611 3704 2169
Size: 249 1193 93 4565
Fragments arranged by size:
4565 1193 249 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3197 3197
Size: 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2247 2458 3040 3282 3371 3419
Size: 399 337 120 211 582 242 89 48
Cuts at: 3419 4161 4246 5317 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 399 337 242 211
120 89 85 48
BspEI T'CCGG_A
Cuts at: 2251 2679 5763 2251
Size: 428 3084 2588
Fragments arranged by size:
3084 2588 428
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2227 3234 3455 844
Size: 1290 93 1007 221 3489
Fragments arranged by size:
3489 1290 1007 221 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2725 2828 3001 3987 1394
Size: 429 902 103 173 986 3507
Fragments arranged by size:
3507 986 902 429 173 103
BsrDI GCAATG_nn'
Cuts at: 65 1248 4595 4769 65
Size: 1183 3347 174 1396
Fragments arranged by size:
3347 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4744 4931 1700
Size: 110 2934 187 2869
Fragments arranged by size:
2934 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2215 794
Size: 1421 4679
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 2083 2098 2758 2968 3270 3430 3797 4182
Size: 111 15 660 210 302 160 367 385
Cuts at: 4182 4199 5651 5737 5749 6095 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 660 385 367 346 302 210
160 111 86 17 15 12
BstZ17I GTA'TAC
Cuts at: 2323 2323
Size: 6100
Bsu36I CC'TnA_GG
Cuts at: 3311 3311
Size: 6100
BtgI C'CryG_G
Cuts at: 513 1065 2034 3108 513
Size: 552 969 1074 3505
Fragments arranged by size:
3505 1074 969 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2033 487
Size: 683 56 807 4554
Fragments arranged by size:
4554 807 683 56
BtsI GCAGTG_nn'
Cuts at: 3616 4410 4430 3616
Size: 794 20 5286
Fragments arranged by size:
5286 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2345 5107 809
Size: 507 1029 2762 1802
Fragments arranged by size:
2762 1802 1029 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2220 3547
Size: 54 1425 50 275 18 181 209 1327
Cuts at: 3547 4436 6065 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1327 889 275 209 181 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 2528 2925 2966 3056 4030 1353
Size: 920 255 397 41 90 974 3423
Fragments arranged by size:
3423 974 920 397 255 90 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2078 2096 4687 5061 5207 387
Size: 1064 138 489 18 2591 374 146 1280
Fragments arranged by size:
2591 1280 1064 489 374 146 138 18
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 6100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2480 2518 2876 3140 4163 4760
Size: 393 662 186 38 358 264 1023 597
Cuts at: 4760 5547 5815 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1023 787 662 597 393 358 268
264 186 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2858 3197 2858
Size: 339 5761
EcoO109I rG'GnC_Cy
Cuts at: 2146 2263 2850 2989 3037 2146
Size: 117 587 139 48 5209
Fragments arranged by size:
5209 587 139 117 48
EcoRI G'AATT_C
Cuts at: 2591 2885 3409 3787 2591
Size: 294 524 378 4904
Fragments arranged by size:
4904 524 378 294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 6100
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 2308 2467 3081 5247 1678
Size: 630 159 614 2166 2531
Fragments arranged by size:
2531 2166 630 614 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3089 3121 4741 4773 4815
Size: 32 135 32 1517 32 1620 32 42
Cuts at: 4815 4847 1373
Size: 32 2626
Fragments arranged by size:
2626 1620 1517 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 3262 3444 3690 669
Size: 988 281 535 789 182 246 3079
Fragments arranged by size:
3079 988 789 535 281 246 182
HindIII A'AGCT_T
Cuts at: 748 2705 748
Size: 1957 4143
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5214 5398 1305
Size: 3909 184 2007
Fragments arranged by size:
3909 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 10
Size: 54 1425 343 390 3888
Fragments arranged by size:
3888 1425 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4058 4417 4576 518
Size: 648 2892 359 159 2042
Fragments arranged by size:
2892 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2036 2057 2188 2538 2639 2812 3034
Size: 423 7 21 131 350 101 173 222
Cuts at: 3034 3118 3327 3516 4193 5341 5586 1606
Size: 84 209 189 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 423 350 245 222 209
189 173 131 101 84 21 7
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5548
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NruI TCG'CGA
Cuts at: 2712 2712
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2282 3458 5003 1132
Size: 97 1053 1176 1545 2229
Fragments arranged by size:
2229 1545 1176 1053 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6100
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6100
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PmlI CAC'GTG
Cuts at: 2844 2844
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 4169 4201 5682 5714 3164
Size: 32 973 32 1481 32 3550
Fragments arranged by size:
3550 1481 973 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2850 2989 2146
Size: 704 139 5257
Fragments arranged by size:
5257 704 139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspOMI G'GGCC_C
Cuts at: 3036 3036
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2044 2211 2979 3452 830
Size: 1214 167 768 473 3478
Fragments arranged by size:
3478 1214 768 473 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2057 2639 3118 3327 2057
Size: 582 479 209 4830
Fragments arranged by size:
4830 582 479 209
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 6100
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SanDI GG'GwC_CC
Cuts at: 2850 2850
Size: 6100
SapI GCTCTTCn'nnn_
Cuts at: 2528 2528
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 2211 3452 2211
Size: 1241 4859
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SexAI A'CCwGG_T
Cuts at: 2475 2475
Size: 6100
SfcI C'TryA_G
Cuts at: 826 1045 2040 2207 2975 3448 4583 5264 5455
Size: 219 995 167 768 473 1135 681 191
Cuts at: 5455 826
Size: 1471
Fragments arranged by size:
1471 1135 995 768 681 473 219 191
167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 4204 5105 5367 820
Size: 197 926 573 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 573 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 2874 1517
Size: 1357 4743
StyI C'CwwG_G
Cuts at: 513 1065 2622 3274 3539 513
Size: 552 1557 652 265 3074
Fragments arranged by size:
3074 1557 652 552 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 4127 4144 4297 4482 4927 325
Size: 1255 839 1708 17 153 185 445 1498
Fragments arranged by size:
1708 1498 1255 839 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3048 3186 4346
Size: 274 80 33 51 1443 1071 138 1160
Cuts at: 4346 6058 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1160 1071 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 6060 6092 3374
Size: 32 2654 32 3382
Fragments arranged by size:
3382 2654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 2413
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 2465 2960 3176 4229 2465
Size: 495 216 1053 4336
Fragments arranged by size:
4336 1053 495 216
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PspXI PsrI RsrII SfiI SfoI SrfI
SwaI