Restriction map for
pFN21AE6422
(Circular) (Six-base) MAPSORT of: pFN21AE6422.seq Check: 2571 from: 1 to: 6208
pFN21AE6422 6208 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 4271 5655 5923 1632
Size: 683 1956 1384 268 1917
Fragments arranged by size:
1956 1917 1384 683 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 3195 1166
Size: 2029 4179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2324 2356 2324
Size: 32 6176
AlwNI CAG_nnn'CTG
Cuts at: 2141 2525 3064 3319 3951 5523 2141
Size: 384 539 255 632 1572 2826
Fragments arranged by size:
2826 1572 632 539 384 255
ApaI G_GGCC'C
Cuts at: 3448 3448
Size: 6208
ApaLI G'TGCA_C
Cuts at: 2973 3045 4265 5421 2973
Size: 72 1220 1156 3760
Fragments arranged by size:
3760 1220 1156 72
ApoI r'AATT_y
Cuts at: 1493 2767 3353 3517 3743 3895 6004 1493
Size: 1274 586 164 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 586 226 164 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2930 3533 1870
Size: 73 987 603 4545
Fragments arranged by size:
4545 987 603 73
BamHI G'GATC_C
Cuts at: 2206 3538 3905 2206
Size: 1332 367 4509
Fragments arranged by size:
4509 1332 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2478 2513 3053 3529 4984
Size: 325 217 627 691 35 540 476 1455
Cuts at: 4984 618
Size: 1842
Fragments arranged by size:
1842 1455 691 627 540 476 325 217
35
BanII G_rGCy'C
Cuts at: 1391 2250 2673 3219 3448 3527 1391
Size: 859 423 546 229 79 4072
Fragments arranged by size:
4072 859 546 423 229 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5237
BbvCI CC'TCA_GC
Cuts at: 2202 2202
Size: 6208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2452 2486 2752 2786 3274
Size: 34 50 34 431 34 266 34 488
Cuts at: 3274 3308 4399 4433 1903
Size: 34 1091 34 3678
Fragments arranged by size:
3678 1091 488 431 266 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2005 4100 5310 2005
Size: 2095 1210 2903
Fragments arranged by size:
2903 2095 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BfrBI ATG'CAT
Cuts at: 2413 2413
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 4819 136
Size: 107 122 71 631 605 1159 1988 1525
Fragments arranged by size:
1988 1525 1159 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2850 6203 2850
Size: 3353 2855
BlpI GC'TnA_GC
Cuts at: 2996 3625 2996
Size: 629 5579
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2977 3049 3058 3448 4269 5425 1790
Size: 445 742 72 9 390 821 1156 2573
Fragments arranged by size:
2573 1156 821 742 445 390 72 9
BmgBI CAC'GTC
Cuts at: 1318 2162 2581 1318
Size: 844 419 4945
Fragments arranged by size:
4945 844 419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2702 2918 3052
Size: 416 53 83 399 24 1285 216 134
Cuts at: 3052 3939 4897 442
Size: 887 958 1753
Fragments arranged by size:
1753 1285 958 887 416 399 216 134
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2103 2504 2744 4868 1239
Size: 864 401 240 2124 2579
Fragments arranged by size:
2579 2124 864 401 240
Bpu10I CC'TnA_GC
Cuts at: 2202 2714 2202
Size: 512 5696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 4333 5198 5496 2831
Size: 1502 865 298 3543
Fragments arranged by size:
3543 1502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2958 4871 882
Size: 617 1459 1913 2219
Fragments arranged by size:
2219 1913 1459 617
BsaAI yAC'GTr
Cuts at: 493 1204 2434 2566 3999 493
Size: 711 1230 132 1433 2702
Fragments arranged by size:
2702 1433 1230 711 132
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4015 4397 5032 275
Size: 53 83 186 936 2482 382 635 1451
Fragments arranged by size:
2482 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2795 2825 1178
Size: 30 165 30 657 30 705 30 4561
Fragments arranged by size:
4561 705 657 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 2228 2681 2741 2948 1186
Size: 855 187 453 60 207 4446
Fragments arranged by size:
4446 855 453 207 187 60
BseYI C'CCAG_C
Cuts at: 2042 2616 3455 5411 5907 2042
Size: 574 839 1956 496 2343
Fragments arranged by size:
2343 1956 839 574 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 3173 1163
Size: 573 888 549 4198
Fragments arranged by size:
4198 888 573 549
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2627 3658 4419 5447 1540
Size: 275 186 626 1031 761 1028 2301
Fragments arranged by size:
2301 1031 1028 761 626 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 2977 3021 3049 3246 4269 4354 5425 2250
Size: 727 44 28 197 1023 85 1071 3033
Fragments arranged by size:
3033 1071 1023 727 197 85 44 28
BsmI GAATG_Cn'
Cuts at: 2253 3719 3812 2253
Size: 1466 93 4649
Fragments arranged by size:
4649 1466 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3119 3119
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2250 2673 2977 3021 3049 3058
Size: 399 445 15 423 304 44 28 9
Cuts at: 3058 3219 3246 3448 3527 4269 4354 5425 1391
Size: 161 27 202 79 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 445 423 399 304 202
161 85 79 44 28 27 15 9
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2550 3563 844
Size: 1655 51 1013 3489
Fragments arranged by size:
3489 1655 1013 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2476 2551 4095 1394
Size: 429 653 75 1544 3507
Fragments arranged by size:
3507 1544 653 429 75
BsrDI GCAATG_nn'
Cuts at: 65 1248 2196 3208 4703 4877 65
Size: 1183 948 1012 1495 174 1396
Fragments arranged by size:
1495 1396 1183 1012 948 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2599 4852 5039 1700
Size: 110 789 2253 187 2869
Fragments arranged by size:
2869 2253 789 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 2976 4268 5280 2976
Size: 1292 1012 3904
Fragments arranged by size:
3904 1292 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3053 794
Size: 2259 3949
BstBI TT'CG_AA
Cuts at: 2905 5012 2905
Size: 2107 4101
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2206 2850 3538 3905 4290 4307 5759 5845
Size: 234 644 688 367 385 17 1452 86
Cuts at: 5845 5857 6203 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 688 644 385 367 346 234
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2443 2968 513
Size: 552 1378 525 3753
Fragments arranged by size:
3753 1378 552 525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2399 487
Size: 683 56 1173 4296
Fragments arranged by size:
4296 1173 683 56
BtsI GCAGTG_nn'
Cuts at: 3477 3724 4518 4538 3477
Size: 247 794 20 5147
Fragments arranged by size:
5147 794 247 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DraIII CAC_nnn'GTG
Cuts at: 3053 3053
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2924 5215 809
Size: 507 1608 2291 1802
Fragments arranged by size:
2291 1802 1608 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2624 3139
Size: 54 1425 50 275 18 393 401 515
Cuts at: 3139 3403 3655 4544 6173 8
Size: 264 252 889 1629 43
Fragments arranged by size:
1629 1425 889 515 401 393 275 264
252 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2624 3655 1537
Size: 275 812 1031 4090
Fragments arranged by size:
4090 1031 812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2789 2795 4138 1353
Size: 1436 6 1343 3423
Fragments arranged by size:
3423 1436 1343 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2586 4795 5169 5315 387
Size: 1064 138 997 2209 374 146 1280
Fragments arranged by size:
2209 1280 1064 997 374 146 138
EcoICRI GAG'CTC
Cuts at: 2248 2248
Size: 6208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2315 2504 2744 4271 4868 5655
Size: 393 471 212 189 240 1527 597 787
Cuts at: 5655 5923 1239
Size: 268 1524
Fragments arranged by size:
1527 1524 787 597 471 393 268 240
212 189
EcoNI CCTnn'n_nnAGG
Cuts at: 3236 3236
Size: 6208
EcoO109I rG'GnC_Cy
Cuts at: 2326 3409 3445 2326
Size: 1083 36 5089
Fragments arranged by size:
5089 1083 36
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FspI TGC'GCA
Cuts at: 2570 4714 2570
Size: 2144 4064
HaeII r_GCGC'y
Cuts at: 1678 2985 5355 1678
Size: 1307 2370 2531
Fragments arranged by size:
2531 2370 1307
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2795 2827 4849
Size: 32 135 32 488 32 703 32 2022
Cuts at: 4849 4881 4923 4955 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2022 703 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2680 3471 3552 3798 669
Size: 988 281 742 791 81 246 3079
Fragments arranged by size:
3079 988 791 742 281 246 81
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 2162 2664 5322 5506 1305
Size: 711 146 502 2658 184 2007
Fragments arranged by size:
2658 2007 711 502 184 146
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 3141 3405 10
Size: 54 1425 343 393 916 264 2813
Fragments arranged by size:
2813 1425 916 393 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3195 4166 4525 4684 518
Size: 648 2029 971 359 159 2042
Fragments arranged by size:
2042 2029 971 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2403 2454 3442 3624 4301 5449 5694 1606
Size: 797 51 988 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 988 797 677 245 182 51
NcoI C'CATG_G
Cuts at: 513 1065 2443 513
Size: 552 1378 4278
Fragments arranged by size:
4278 1378 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NsiI A_TGCA'T
Cuts at: 2415 2415
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2413 2467 2838 3331 3566 5111 1132
Size: 97 1184 54 371 493 235 1545 2229
Fragments arranged by size:
2229 1545 1184 493 371 235 97 54
PasI CC'CwG_GG
Cuts at: 1348 2166 1348
Size: 818 5390
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3064 1108
Size: 1956 4252
PfoI T'CCnGG_A
Cuts at: 2150 2307 3318 3348 2150
Size: 157 1011 30 5010
Fragments arranged by size:
5010 1011 157 30
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PmlI CAC'GTG
Cuts at: 2434 2434
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2326 2326
Size: 6208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 3444 3444
Size: 6208
PspXI vC'TCGA_Gb
Cuts at: 2930 2930
Size: 6208
PstI C_TGCA'G
Cuts at: 830 3560 830
Size: 2730 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2454 2454
Size: 6208
SacI G_AGCT'C
Cuts at: 2250 2250
Size: 6208
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SexAI A'CCwGG_T
Cuts at: 2736 3036 2736
Size: 300 5908
SfcI C'TryA_G
Cuts at: 826 1045 2108 3556 4691 5372 5563 826
Size: 219 1063 1448 1135 681 191 1471
Fragments arranged by size:
1471 1448 1135 1063 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 2599 1700
Size: 899 5309
SmaI CCC'GGG
Cuts at: 1872 3535 1872
Size: 1663 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 2930 4312 5213 5475 820
Size: 197 926 903 84 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 903 901 262 197 84
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 2413 2838 3566 1132
Size: 1281 425 728 3774
Fragments arranged by size:
3774 1281 728 425
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2443 2814 3647 513
Size: 552 1378 371 833 3074
Fragments arranged by size:
3074 1378 833 552 371
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 2937 4235 4252 4405 4590 5035
Size: 1255 498 859 1298 17 153 185 445
Cuts at: 5035 325
Size: 1498
Fragments arranged by size:
1498 1298 1255 859 498 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4454 6166 96
Size: 274 80 33 51 1443 2477 1712 138
Fragments arranged by size:
2477 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 6168 6200 2198
Size: 32 3938 32 2206
Fragments arranged by size:
3938 2206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 2930 1943
Size: 987 5221
XmaI C'CCGG_G
Cuts at: 1870 3533 1870
Size: 1663 4545
XmnI GAAnn'nnTTC
Cuts at: 2783 4337 2783
Size: 1554 4654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI
BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI