Restriction map for
pFN21AE6421
(Circular) (Six-base) MAPSORT of: pFN21AE6421.seq Check: 3584 from: 1 to: 6379
pFN21AE6421 6379 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2158 2158
Size: 6379
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2049 3200 3722 1937
Size: 112 1151 522 4594
Fragments arranged by size:
4594 1151 522 112
Acc65I G'GTAC_C
Cuts at: 3604 3700 3604
Size: 96 6283
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2356 4442 5826 6094 1632
Size: 724 2086 1384 268 1917
Fragments arranged by size:
2086 1917 1384 724 268
AfeI AGC'GCT
Cuts at: 2702 3297 2702
Size: 595 5784
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 3249 3251 5278 6255 1203
Size: 2046 2 2027 977 1327
Fragments arranged by size:
2046 2027 1327 977 2
AgeI A'CCGG_T
Cuts at: 3601 3629 5210 3601
Size: 28 1581 4770
Fragments arranged by size:
4770 1581 28
AhdI GACnn_n'nnGTC
Cuts at: 2431 2647 2917 5108 2431
Size: 216 270 2191 3702
Fragments arranged by size:
3702 2191 270 216
AleI CACnn'nnGTG
Cuts at: 1166 2855 3236 1166
Size: 1689 381 4309
Fragments arranged by size:
4309 1689 381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3125 1184
Size: 1941 4438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 2201
Size: 32 6347
AlwNI CAG_nnn'CTG
Cuts at: 2614 3480 4122 5694 2614
Size: 866 642 1572 3299
Fragments arranged by size:
3299 1572 866 642
ApaI G_GGCC'C
Cuts at: 3543 3543
Size: 6379
ApaLI G'TGCA_C
Cuts at: 2126 4436 5592 2126
Size: 2310 1156 2913
Fragments arranged by size:
2913 2310 1156
ApoI r'AATT_y
Cuts at: 1493 2549 3688 3914 4066 6175 1493
Size: 1056 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 1056 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2180 3347 3704 1870
Size: 73 114 123 1167 357 4545
Fragments arranged by size:
4545 1167 357 123 114 73
BamHI G'GATC_C
Cuts at: 3709 4076 3709
Size: 367 6012
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2321 2395 2857 3037
Size: 325 217 627 383 151 74 462 180
Cuts at: 3037 3400 3604 3700 5155 618
Size: 363 204 96 1455 1842
Fragments arranged by size:
1842 1455 627 462 383 363 325 217
204 180 151 96 74
BanII G_rGCy'C
Cuts at: 1391 2039 2766 2869 2877 3492 3543 3663 3698
Size: 648 727 103 8 615 51 120 35
Cuts at: 3698 1391
Size: 4072
Fragments arranged by size:
4072 727 648 615 120 103 51 35
8
BbeI G_GCGC'C
Cuts at: 2399 3041 3404 2399
Size: 642 363 5374
Fragments arranged by size:
5374 642 363
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3442 3476 4570 4604 1903
Size: 34 1505 34 1094 34 3678
Fragments arranged by size:
3678 1505 1094 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4271 5481 4271
Size: 1210 5169
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2069 4990
Size: 107 122 71 631 605 337 60 2921
Cuts at: 4990 136
Size: 1525
Fragments arranged by size:
2921 1525 631 605 337 122 107 71
60
BglII A'GATC_T
Cuts at: 6374 6374
Size: 6379
BlpI GC'TnA_GC
Cuts at: 3383 3796 3383
Size: 413 5966
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2175 2324 2862 3543 4440 5596 1790
Size: 340 45 149 538 681 897 1156 2573
Fragments arranged by size:
2573 1156 897 681 538 340 149 45
BmgBI CAC'GTC
Cuts at: 1318 2044 2136 1318
Size: 726 92 5561
Fragments arranged by size:
5561 726 92
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2014 2488 3101
Size: 416 53 83 399 24 597 474 613
Cuts at: 3101 4110 5068 442
Size: 1009 958 1753
Fragments arranged by size:
1753 1009 958 613 597 474 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3220 3220
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2636 2792 2834 5039 1239
Size: 1397 156 42 2205 2579
Fragments arranged by size:
2579 2205 1397 156 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2891 2960 3533 4504 5369 5667 2105
Size: 786 69 573 971 865 298 2817
Fragments arranged by size:
2817 971 865 786 573 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2844 5042 882
Size: 617 1345 2198 2219
Fragments arranged by size:
2219 2198 1345 617
BsaAI yAC'GTr
Cuts at: 493 1204 3169 3252 4170 493
Size: 711 1965 83 918 2702
Fragments arranged by size:
2702 1965 918 711 83
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2343 2396 3038 3401
Size: 53 83 186 936 810 53 642 363
Cuts at: 3401 4186 4568 5203 275
Size: 785 382 635 1451
Fragments arranged by size:
1451 936 810 785 642 635 382 363
186 83 53 53
BsaWI w'CCGG_w
Cuts at: 2085 2154 2646 3601 3629 4812 5210 5484 5631
Size: 69 492 955 28 1183 398 274 147
Cuts at: 5631 6042 2085
Size: 411 2422
Fragments arranged by size:
2422 1183 955 492 411 398 274 147
69 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 2483 2513 1178
Size: 30 165 30 798 30 252 30 5044
Fragments arranged by size:
5044 798 252 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3671 1186
Size: 2485 3894
BseYI C'CCAG_C
Cuts at: 2199 2421 3217 3542 5582 6078 2199
Size: 222 796 325 2040 496 2500
Fragments arranged by size:
2500 2040 796 496 325 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3116 3227 3316 3610 1163
Size: 573 1380 111 89 294 3932
Fragments arranged by size:
3932 1380 573 294 111 89
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2281 3829 4590 5618 1540
Size: 275 186 280 1548 761 1028 2301
Fragments arranged by size:
2301 1548 1028 761 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2766 2869 2877 3114 4440 4525 5596 2130
Size: 636 103 8 237 1326 85 1071 2913
Fragments arranged by size:
2913 1326 1071 636 237 103 85 8
BsiWI C'GTAC_G
Cuts at: 3165 3165
Size: 6379
BsmI GAATG_Cn'
Cuts at: 3890 3983 3890
Size: 93 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2130 2175 2324 2766 2862 2869
Size: 399 249 91 45 149 442 96 7
Cuts at: 2869 2877 3114 3492 3543 3663 3698 4440 4525
Size: 8 237 378 51 120 35 742 85
Cuts at: 4525 5596 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 378 249 237
149 120 96 91 85 51 45 35
8 7
BspEI T'CCGG_A
Cuts at: 2154 6042 2154
Size: 3888 2491
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 2754 3734 844
Size: 1314 596 980 3489
Fragments arranged by size:
3489 1314 980 596
BsrBI CCG'CTC
Cuts at: 1394 1823 3054 4266 1394
Size: 429 1231 1212 3507
Fragments arranged by size:
3507 1231 1212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3041 4874 5048 65
Size: 1183 1793 1833 174 1396
Fragments arranged by size:
1833 1793 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3601 3629 5023 5210 1700
Size: 110 1791 28 1394 187 2869
Fragments arranged by size:
2869 1791 1394 187 110 28
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6379
BssHII G'CGCG_C
Cuts at: 1925 2461 2571 2573 2575 3184 3186 1925
Size: 536 110 2 2 609 2 5118
Fragments arranged by size:
5118 609 536 110 2 2 2
BssSI C'ACGA_G
Cuts at: 2408 4439 5451 2408
Size: 2031 1012 3336
Fragments arranged by size:
3336 2031 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6379
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 3235 3286 2812
Size: 423 51 5905
Fragments arranged by size:
5905 423 51
BstYI r'GATC_y
Cuts at: 1972 2622 3709 4076 4461 4478 5930 6016 6028
Size: 650 1087 367 385 17 1452 86 12
Cuts at: 6028 6374 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1087 650 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3201 3201
Size: 6379
Bsu36I CC'TnA_GG
Cuts at: 3473 3473
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2004 2232 2388 2799 3564 513
Size: 552 939 228 156 411 765 3328
Fragments arranged by size:
3328 939 765 552 411 228 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2468 2539 487
Size: 683 56 1242 71 4327
Fragments arranged by size:
4327 1242 683 71 56
BtsI GCAGTG_nn'
Cuts at: 3139 3895 4689 4709 3139
Size: 756 794 20 4809
Fragments arranged by size:
4809 794 756 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DraIII CAC_nnn'GTG
Cuts at: 3145 3370 3145
Size: 225 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2278 2445
Size: 54 1425 50 275 18 411 37 167
Cuts at: 2445 2501 2559 2802 3270 3826 4715 6344 8
Size: 56 58 243 468 556 889 1629 43
Fragments arranged by size:
1629 1425 889 556 468 411 275 243
167 58 56 54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2278 3826 1537
Size: 275 466 1548 4090
Fragments arranged by size:
4090 1548 466 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2798 3123 3398 4309 1353
Size: 1445 325 275 911 3423
Fragments arranged by size:
3423 1445 911 325 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2919 3237 3434 4966 5340 5486
Size: 1064 138 1330 318 197 1532 374 146
Cuts at: 5486 387
Size: 1280
Fragments arranged by size:
1532 1330 1280 1064 374 318 197 146
138
EcoICRI GAG'CTC
Cuts at: 2764 2867 2875 2764
Size: 103 8 6268
Fragments arranged by size:
6268 103 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 2636 2792 2834 4442 5039 5826
Size: 393 724 280 156 42 1608 597 787
Cuts at: 5826 6094 1239
Size: 268 1524
Fragments arranged by size:
1608 1524 787 724 597 393 280 268
156 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2447 3395 2447
Size: 948 5431
EcoO109I rG'GnC_Cy
Cuts at: 2515 3477 3539 2515
Size: 962 62 5355
Fragments arranged by size:
5355 962 62
EcoRI G'AATT_C
Cuts at: 2549 3688 4066 2549
Size: 1139 378 4862
Fragments arranged by size:
4862 1139 378
FspI TGC'GCA
Cuts at: 2607 2753 4885 2607
Size: 146 2132 4101
Fragments arranged by size:
4101 2132 146
HaeII r_GCGC'y
Cuts at: 1678 2399 2704 3041 3299 3404 3535 5526 1678
Size: 721 305 337 258 105 131 1991 2531
Fragments arranged by size:
2531 1991 721 337 305 258 131 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 2633 2665 2903
Size: 32 135 32 845 32 184 32 238
Cuts at: 2903 2935 3017 3049 5020 5052 5094 5126 1373
Size: 32 82 32 1971 32 42 32 2626
Fragments arranged by size:
2626 1971 845 238 184 135 82 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3723 3969 669
Size: 988 281 1785 246 3079
Fragments arranged by size:
3079 1785 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KasI G'GCGC_C
Cuts at: 2395 3037 3400 2395
Size: 642 363 5374
Fragments arranged by size:
5374 642 363
KpnI G_GTAC'C
Cuts at: 3608 3704 3608
Size: 96 6283
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3538 5493 5677 1305
Size: 2233 1955 184 2007
Fragments arranged by size:
2233 2007 1955 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2243 2804 10
Size: 54 1425 343 411 561 3585
Fragments arranged by size:
3585 1425 561 411 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 3236 3338 4337 4696 4855 518
Size: 648 1689 381 102 999 359 159 2042
Fragments arranged by size:
2042 1689 999 648 381 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2234 2277 2614 3456 3611 3795 4472 5620
Size: 628 43 337 842 155 184 677 1148
Cuts at: 5620 5865 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 842 677 628 337 245 184
155 43
NarI GG'CG_CC
Cuts at: 2396 3038 3401 2396
Size: 642 363 5374
Fragments arranged by size:
5374 642 363
NcoI C'CATG_G
Cuts at: 513 1065 2004 2388 513
Size: 552 939 384 4504
Fragments arranged by size:
4504 939 552 384
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 2278 3826 2278
Size: 1548 4831
NspI r_CATG'y
Cuts at: 1132 1229 3511 3737 5282 1132
Size: 97 2282 226 1545 2229
Fragments arranged by size:
2282 2229 1545 226 97
PasI CC'CwG_GG
Cuts at: 1348 3536 1348
Size: 2188 4191
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3145 1108
Size: 2037 4342
PfoI T'CCnGG_A
Cuts at: 2811 3513 2811
Size: 702 5677
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PmlI CAC'GTG
Cuts at: 3252 3252
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2201 2233 4448 4480 5961 5993 2201
Size: 32 2215 32 1481 32 2587
Fragments arranged by size:
2587 2215 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2096 2933 1613
Size: 483 837 5059
Fragments arranged by size:
5059 837 483
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PspOMI G'GGCC_C
Cuts at: 3539 3539
Size: 6379
PstI C_TGCA'G
Cuts at: 830 2490 3731 830
Size: 1660 1241 3478
Fragments arranged by size:
3478 1660 1241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 2614 3456 2614
Size: 842 5537
RsrII CG'GwC_CG
Cuts at: 2157 2157
Size: 6379
SacI G_AGCT'C
Cuts at: 2766 2869 2877 2766
Size: 103 8 6268
Fragments arranged by size:
6268 103 8
SacII CC_GC'GG
Cuts at: 2235 2235
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SapI GCTCTTCn'nnn_
Cuts at: 2798 3123 2798
Size: 325 6054
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 2486 3727 4862 5543 5734 826
Size: 219 1441 1241 1135 681 191 1471
Fragments arranged by size:
1471 1441 1241 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2397 3039 3402 2397
Size: 642 363 5374
Fragments arranged by size:
5374 642 363
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 3629 1700
Size: 1929 4450
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2870 2975 3548 4483 5384
Size: 197 926 177 750 105 573 935 901
Cuts at: 5384 5646 820
Size: 262 1553
Fragments arranged by size:
1553 935 926 901 750 573 262 197
177 105
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 3406 152
Size: 3254 3125
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 2724 1517
Size: 1207 5172
StyI C'CwwG_G
Cuts at: 513 1065 2004 2388 3818 513
Size: 552 939 384 1430 3074
Fragments arranged by size:
3074 1430 939 552 384
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2756 3161 3292 3320 4406 4423 4576
Size: 1255 1176 405 131 28 1086 17 153
Cuts at: 4576 4761 5206 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1176 1086 445 405 185 153
131 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4625 6337 96
Size: 274 80 33 51 1443 2648 1712 138
Fragments arranged by size:
2648 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2120 2152 2483 2515 6339 6371 2120
Size: 32 331 32 3824 32 2128
Fragments arranged by size:
3824 2128 331 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2045 2669 2983 1316
Size: 729 624 314 4712
Fragments arranged by size:
4712 729 624 314
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3076
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 4508 4508
Size: 6379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI Bpu10I BsmBI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PpuMI PspXI PsrI SanDI SexAI SfiI SrfI SwaI