Restriction map for
pFN21AE6420
(Circular) (Six-base) MAPSORT of: pFN21AE6420.seq Check: 4075 from: 1 to: 6226
pFN21AE6420 6226 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2730 4036 278
Size: 53 83 186 936 1194 1306 2468
Fragments arranged by size:
2468 1306 1194 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2175 2892 3569 1937
Size: 238 717 677 4594
Fragments arranged by size:
4594 717 677 238
Acc65I G'GTAC_C
Cuts at: 3547 3547
Size: 6226
AclI AA'CG_TT
Cuts at: 1563 4353 4726 1563
Size: 2790 373 3063
Fragments arranged by size:
3063 2790 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4289 5673 5941 1632
Size: 2657 1384 268 1917
Fragments arranged by size:
2657 1917 1384 268
AfeI AGC'GCT
Cuts at: 2207 2207
Size: 6226
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6029
AflIII A'CryG_T
Cuts at: 1203 2472 2817 5125 6102 1203
Size: 1269 345 2308 977 1327
Fragments arranged by size:
2308 1327 1269 977 345
AgeI A'CCGG_T
Cuts at: 5057 5057
Size: 6226
AhdI GACnn_n'nnGTC
Cuts at: 4955 4955
Size: 6226
AleI CACnn'nnGTG
Cuts at: 1166 3191 1166
Size: 2025 4201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3041 1184
Size: 1857 4369
AlwNI CAG_nnn'CTG
Cuts at: 2866 3163 3969 5541 2866
Size: 297 806 1572 3551
Fragments arranged by size:
3551 1572 806 297
ApaI G_GGCC'C
Cuts at: 2036 2812 2036
Size: 776 5450
ApaLI G'TGCA_C
Cuts at: 4283 5439 4283
Size: 1156 5070
ApoI r'AATT_y
Cuts at: 1493 3535 3761 3913 6022 1493
Size: 2042 226 152 2109 1697
Fragments arranged by size:
2109 2042 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4780 160
Size: 872 3748 1606
Fragments arranged by size:
3748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2673 3455 3551 1870
Size: 73 111 619 782 96 4545
Fragments arranged by size:
4545 782 619 111 96 73
BamHI G'GATC_C
Cuts at: 2215 2543 3556 3923 2215
Size: 328 1013 367 4518
Fragments arranged by size:
4518 1013 367 328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2608 3193 3391 3547 5002
Size: 325 217 627 821 585 198 156 1455
Cuts at: 5002 618
Size: 1842
Fragments arranged by size:
1842 1455 821 627 585 325 217 198
156
BanII G_rGCy'C
Cuts at: 1391 2036 2448 2509 2812 3110 3312 3545 1391
Size: 645 412 61 303 298 202 233 4072
Fragments arranged by size:
4072 645 412 303 298 233 202 61
BbeI G_GCGC'C
Cuts at: 2612 2612
Size: 6226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5255
BbvCI CC'TCA_GC
Cuts at: 2799 2799
Size: 6226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4417 4451 1903
Size: 34 50 34 2396 34 3678
Fragments arranged by size:
3678 2396 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4118 5328 4118
Size: 1210 5016
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2606 2699 4837
Size: 107 122 71 631 605 934 93 2138
Cuts at: 4837 136
Size: 1525
Fragments arranged by size:
2138 1525 934 631 605 122 107 93
71
BglII A'GATC_T
Cuts at: 6221 6221
Size: 6226
BlpI GC'TnA_GC
Cuts at: 2333 2636 3643 2333
Size: 303 1007 4916
Fragments arranged by size:
4916 1007 303
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2812 3198 3292 3456 4287 5443 1790
Size: 246 776 386 94 164 831 1156 2573
Fragments arranged by size:
2573 1156 831 776 386 246 164 94
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2749 3957 4915
Size: 416 53 83 399 24 1332 1208 958
Cuts at: 4915 442
Size: 1753
Fragments arranged by size:
1753 1332 1208 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 3371 3497 4886 1239
Size: 983 1149 126 1389 2579
Fragments arranged by size:
2579 1389 1149 983 126
Bpu10I CC'TnA_GC
Cuts at: 2799 2799
Size: 6226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4351 5216 5514 4351
Size: 865 298 5063
Fragments arranged by size:
5063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3452 4889 882
Size: 617 1953 1437 2219
Fragments arranged by size:
2219 1953 1437 617
BsaAI yAC'GTr
Cuts at: 493 1204 2473 2818 4017 493
Size: 711 1269 345 1199 2702
Fragments arranged by size:
2702 1269 1199 711 345
BsaBI GATnn'nnATC
Cuts at: 6220 6220
Size: 6226
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2609 2727 4033 4415
Size: 53 83 186 936 1076 118 1306 382
Cuts at: 4415 5050 275
Size: 635 1451
Fragments arranged by size:
1451 1306 1076 936 635 382 186 118
83 53
BsaWI w'CCGG_w
Cuts at: 2769 4659 5057 5331 5478 5889 2769
Size: 1890 398 274 147 411 3106
Fragments arranged by size:
3106 1890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6001
Fragments arranged by size:
6001 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6226
BseYI C'CCAG_C
Cuts at: 2020 2194 2523 3240 5429 5925 2020
Size: 174 329 717 2189 496 2321
Fragments arranged by size:
2321 2189 717 496 329 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5653
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2286 2367 3676 4437 5465 1540
Size: 275 186 285 81 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 285 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2145 2619 3377 4287 4372 5443 2145
Size: 474 758 910 85 1071 2928
Fragments arranged by size:
2928 1071 910 758 474 85
BsmI GAATG_Cn'
Cuts at: 3737 3830 3737
Size: 93 6133
BsmBI CGTCTCn'nnnn_
Cuts at: 3040 3040
Size: 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2145 2448 2509 2619 2812 3110
Size: 399 246 109 303 61 110 193 298
Cuts at: 3110 3198 3292 3312 3377 3456 3545 4287 4372
Size: 88 94 20 65 79 89 742 85
Cuts at: 4372 5443 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 298 246 193
110 109 94 89 88 85 79 65
61 20
BspEI T'CCGG_A
Cuts at: 2769 5889 2769
Size: 3120 3106
BspHI T'CATG_A
Cuts at: 2622 4115 5845 2622
Size: 1493 1730 3003
Fragments arranged by size:
3003 1730 1493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 2904 3581 844
Size: 1388 672 677 3489
Fragments arranged by size:
3489 1388 677 672
BsrBI CCG'CTC
Cuts at: 1394 1823 4113 1394
Size: 429 2290 3507
Fragments arranged by size:
3507 2290 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4721 4895 65
Size: 1183 3473 174 1396
Fragments arranged by size:
3473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2366 2823 4870 5057 1700
Size: 110 556 457 2047 187 2869
Fragments arranged by size:
2869 2047 556 457 187 110
BsrGI T'GTAC_A
Cuts at: 96 3078 96
Size: 2982 3244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6226
BssSI C'ACGA_G
Cuts at: 4286 5298 4286
Size: 1012 5214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6226
BstBI TT'CG_AA
Cuts at: 5030 5030
Size: 6226
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6226
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 3421 2836
Size: 585 5641
BstYI r'GATC_y
Cuts at: 1972 2215 2543 2754 3556 3923 4308 4325 5777
Size: 243 328 211 802 367 385 17 1452
Cuts at: 5777 5863 5875 6221 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 802 385 367 346 328 243
211 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3304 3304
Size: 6226
BtgI C'CryG_G
Cuts at: 513 1065 2579 2835 3000 3234 3292 513
Size: 552 1514 256 165 234 58 3447
Fragments arranged by size:
3447 1514 552 256 234 165 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5487
Fragments arranged by size:
5487 683 56
BtsI GCAGTG_nn'
Cuts at: 2145 2541 3117 3742 4536 4556 2145
Size: 396 576 625 794 20 3815
Fragments arranged by size:
3815 794 625 576 396 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6191
DraI TTT'AAA
Cuts at: 3530 3530
Size: 6226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2732 5233 809
Size: 507 1416 2501 1802
Fragments arranged by size:
2501 1802 1416 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2283 2364
Size: 54 1425 50 275 18 294 159 81
Cuts at: 2364 2598 3042 3204 3321 3411 3673 4562 6191
Size: 234 444 162 117 90 262 889 1629
Cuts at: 6191 8
Size: 43
Fragments arranged by size:
1629 1425 889 444 294 275 262 234
162 159 117 90 81 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2283 2364 3673 1537
Size: 275 471 81 1309 4090
Fragments arranged by size:
4090 1309 471 275 81
EarI CTCTTCn'nnn_
Cuts at: 1353 2682 3035 3221 4156 1353
Size: 1329 353 186 935 3423
Fragments arranged by size:
3423 1329 935 353 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4813 5187 5333 387
Size: 1064 138 3224 374 146 1280
Fragments arranged by size:
3224 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 3371 3497 4289 4886 5673 5941
Size: 393 590 1149 126 792 597 787 268
Cuts at: 5941 1239
Size: 1524
Fragments arranged by size:
1524 1149 792 787 597 590 393 268
126
EcoO109I rG'GnC_Cy
Cuts at: 2032 2097 2692 2809 3245 3274 3513 2032
Size: 65 595 117 436 29 239 4745
Fragments arranged by size:
4745 595 436 239 117 65 29
EcoRI G'AATT_C
Cuts at: 3535 3913 3535
Size: 378 5848
FspI TGC'GCA
Cuts at: 2391 4732 2391
Size: 2341 3885
FspAI rTGC'GCAy
Cuts at: 2391 2391
Size: 6226
HaeII r_GCGC'y
Cuts at: 1678 2209 2587 2612 2705 5373 1678
Size: 531 378 25 93 2668 2531
Fragments arranged by size:
2668 2531 531 378 93 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4867 4899 4941 4973 1373
Size: 32 135 32 3295 32 42 32 2626
Fragments arranged by size:
3295 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3570 3816 669
Size: 988 281 1632 246 3079
Fragments arranged by size:
3079 1632 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6226
HpaI GTT'AAC
Cuts at: 3816 3816
Size: 6226
KasI G'GCGC_C
Cuts at: 2608 2608
Size: 6226
KpnI G_GTAC'C
Cuts at: 3551 3551
Size: 6226
MfeI C'AATT_G
Cuts at: 3825 3825
Size: 6226
MluI A'CGCG_T
Cuts at: 6102 6102
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5340 5524 1305
Size: 4035 184 2007
Fragments arranged by size:
4035 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 3206 3323 10
Size: 54 1425 343 294 1080 117 2913
Fragments arranged by size:
2913 1425 1080 343 294 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2534 2834 3191 4184 4543 4702
Size: 648 1299 69 300 357 993 359 159
Cuts at: 4702 518
Size: 2042
Fragments arranged by size:
2042 1299 993 648 359 357 300 159
69
MspA1I CmG'CkG
Cuts at: 1606 2017 2282 2351 2453 2866 2921 3041 3176
Size: 411 265 69 102 413 55 120 135
Cuts at: 3176 3327 3642 4319 5467 5712 1606
Size: 151 315 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 413 411 315 265 245
151 135 120 102 69 55
NaeI GCC'GGC
Cuts at: 2368 2825 2368
Size: 457 5769
NarI GG'CG_CC
Cuts at: 2609 2609
Size: 6226
NcoI C'CATG_G
Cuts at: 513 1065 3000 513
Size: 552 1935 3739
Fragments arranged by size:
3739 1935 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6226
NgoMIV G'CCGG_C
Cuts at: 2366 2823 2366
Size: 457 5769
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6226
NotI GC'GGCC_GC
Cuts at: 3673 3673
Size: 6226
NspI r_CATG'y
Cuts at: 1132 1229 3584 5129 1132
Size: 97 2355 1545 2229
Fragments arranged by size:
2355 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2272 1348
Size: 924 5302
PciI A'CATG_T
Cuts at: 5125 5125
Size: 6226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 3255 1108
Size: 1422 725 4079
Fragments arranged by size:
4079 1422 725
PfoI T'CCnGG_A
Cuts at: 2757 2757
Size: 6226
PmeI GTTT'AAAC
Cuts at: 3530 3530
Size: 6226
PmlI CAC'GTG
Cuts at: 2818 2818
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5808 5840 4295
Size: 32 1481 32 4681
Fragments arranged by size:
4681 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 3245 3274 3513 2097
Size: 1148 29 239 4810
Fragments arranged by size:
4810 1148 239 29
PshAI GACnn'nnGTC
Cuts at: 1613 2726 1613
Size: 1113 5113
PsiI TTA'TAA
Cuts at: 3796 3796
Size: 6226
PspOMI G'GGCC_C
Cuts at: 2032 2808 2032
Size: 776 5450
PstI C_TGCA'G
Cuts at: 830 2106 2862 2901 3578 830
Size: 1276 756 39 677 3478
Fragments arranged by size:
3478 1276 756 677 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6226
PvuII CAG'CTG
Cuts at: 2351 2453 2866 3327 2351
Size: 102 413 461 5250
Fragments arranged by size:
5250 461 413 102
SalI G'TCGA_C
Cuts at: 1936 3568 1936
Size: 1632 4594
SanDI GG'GwC_CC
Cuts at: 2097 3245 2097
Size: 1148 5078
SapI GCTCTTCn'nnn_
Cuts at: 3035 3221 3035
Size: 186 6040
SbfI CC_TGCA'GG
Cuts at: 3578 3578
Size: 6226
ScaI AGT'ACT
Cuts at: 4474 6186 4474
Size: 1712 4514
SfcI C'TryA_G
Cuts at: 826 1045 2102 2858 2897 3574 4709 5390 5581
Size: 219 1057 756 39 677 1135 681 191
Cuts at: 5581 826
Size: 1471
Fragments arranged by size:
1471 1135 1057 756 681 677 219 191
39
SfoI GGC'GCC
Cuts at: 2610 2610
Size: 6226
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6226
SmaI CCC'GGG
Cuts at: 1872 3553 1872
Size: 1681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4330 5231 5493 820
Size: 197 926 2387 901 262 1553
Fragments arranged by size:
2387 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4017 493
Size: 3524 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6226
SphI G_CATG'C
Cuts at: 1132 3584 1132
Size: 2452 3774
SspI AAT'ATT
Cuts at: 5 52 4150 5
Size: 47 4098 2081
Fragments arranged by size:
4098 2081 47
StuI AGG'CCT
Cuts at: 1517 2292 3100 1517
Size: 775 808 4643
Fragments arranged by size:
4643 808 775
StyI C'CwwG_G
Cuts at: 513 1065 2211 2563 2937 3000 3462 3665 513
Size: 552 1146 352 374 63 462 203 3074
Fragments arranged by size:
3074 1146 552 462 374 352 203 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4253 4270 4423 4608 5053 325
Size: 1255 2673 17 153 185 445 1498
Fragments arranged by size:
2673 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3078 4472 6184
Size: 274 80 33 51 1443 1101 1394 1712
Cuts at: 6184 96
Size: 138
Fragments arranged by size:
1712 1443 1394 1101 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6186 6218 6186
Size: 32 6194
Tth111I GACn'n_nGTC
Cuts at: 1316 2765 1316
Size: 1449 4777
XbaI T'CTAG_A
Cuts at: 3562 3562
Size: 6226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3067
Size: 6226
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6226
XmaI C'CCGG_G
Cuts at: 1870 3551 1870
Size: 1681 4545
XmnI GAAnn'nnTTC
Cuts at: 4355 4355
Size: 6226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2728 4034 276
Size: 53 83 186 936 1194 1306 2468
Fragments arranged by size:
2468 1306 1194 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SexAI
SfiI SrfI SwaI