Restriction map for
pFN21AE6419
(Circular) (Six-base) MAPSORT of: pFN21AE6419.seq Check: 1839 from: 1 to: 6193
pFN21AE6419 6193 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 2217 3514 2217
Size: 1297 4896
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4256 5640 5908 1632
Size: 2624 1384 268 1917
Fragments arranged by size:
2624 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2080 820
Size: 197 1063 4933
Fragments arranged by size:
4933 1063 197
AflIII A'CryG_T
Cuts at: 1203 3138 5092 6069 1203
Size: 1935 1954 977 1327
Fragments arranged by size:
1954 1935 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 3298 4922 3298
Size: 1624 4569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2780 1184
Size: 1596 4597
AlwNI CAG_nnn'CTG
Cuts at: 2507 2612 2724 3936 5508 2507
Size: 105 112 1212 1572 3192
Fragments arranged by size:
3192 1572 1212 112 105
ApaI G_GGCC'C
Cuts at: 2089 3367 2089
Size: 1278 4915
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2431 3502 3728 3880 5989 1493
Size: 938 1071 226 152 2109 1697
Fragments arranged by size:
2109 1697 1071 938 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2736 4747 160
Size: 872 1704 2011 1606
Fragments arranged by size:
2011 1704 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 3518 1870
Size: 73 1575 4545
Fragments arranged by size:
4545 1575 73
BamHI G'GATC_C
Cuts at: 3400 3523 3890 3400
Size: 123 367 5703
Fragments arranged by size:
5703 367 123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2217 3514 4969 618
Size: 325 217 627 260 170 1297 1455 1842
Fragments arranged by size:
1842 1455 1297 627 325 260 217 170
BanII G_rGCy'C
Cuts at: 1391 2020 2089 2602 2785 3367 3512 1391
Size: 629 69 513 183 582 145 4072
Fragments arranged by size:
4072 629 582 513 183 145 69
BbeI G_GCGC'C
Cuts at: 2051 2051
Size: 6193
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5222
BbvCI CC'TCA_GC
Cuts at: 2334 2334
Size: 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 2176 1548
Size: 628 5565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 2914 4804
Size: 107 122 71 631 605 412 830 1890
Cuts at: 4804 136
Size: 1525
Fragments arranged by size:
1890 1525 830 631 605 412 122 107
71
BglII A'GATC_T
Cuts at: 3376 6188 3376
Size: 2812 3381
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 2089 3367 4254 5410 1790
Size: 299 1278 887 1156 2573
Fragments arranged by size:
2573 1278 1156 887 299
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3083 3924 4882
Size: 416 53 83 399 24 1666 841 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1666 958 841 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2405 2405
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2799 4853 1239
Size: 1560 2054 2579
Fragments arranged by size:
2579 2054 1560
Bpu10I CC'TnA_GC
Cuts at: 2334 2334
Size: 6193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3199 4856 882
Size: 617 1700 1657 2219
Fragments arranged by size:
2219 1700 1657 617
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 4000 4382 5017
Size: 53 83 186 936 515 1952 382 635
Cuts at: 5017 275
Size: 1451
Fragments arranged by size:
1952 1451 936 635 515 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3297 3327 1178
Size: 30 165 30 1894 30 4044
Fragments arranged by size:
4044 1894 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6193
BseYI C'CCAG_C
Cuts at: 5396 5892 5396
Size: 496 5697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3079 1163
Size: 573 1343 4277
Fragments arranged by size:
4277 1343 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2075 3643 4404 5432 1540
Size: 275 186 74 1568 761 1028 2301
Fragments arranged by size:
2301 1568 1028 761 275 186 74
BsiHKAI G_wGCw'C
Cuts at: 2602 4254 4339 5410 2602
Size: 1652 85 1071 3385
Fragments arranged by size:
3385 1652 1071 85
BsmI GAATG_Cn'
Cuts at: 3397 3704 3797 3397
Size: 307 93 5793
Fragments arranged by size:
5793 307 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2089 2602 2785 3367 3512 4254
Size: 399 230 69 513 183 582 145 742
Cuts at: 4254 4339 5410 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 513 399 230 183
145 85 69
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3548 844
Size: 2704 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 4080 1394
Size: 429 1603 654 3507
Fragments arranged by size:
3507 1603 654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 2871 3286 4688 4862 65
Size: 1183 1275 348 415 1402 174 1396
Fragments arranged by size:
1402 1396 1275 1183 415 348 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 4837 5024 1700
Size: 110 264 2763 187 2869
Fragments arranged by size:
2869 2763 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2612 794
Size: 1818 4375
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 2838 3376 3400 3523 3890 4275 4292 5744
Size: 866 538 24 123 367 385 17 1452
Cuts at: 5744 5830 5842 6188 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 866 538 385 367 346 123
86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2764 2774 2966 3709 4503 4523 2764
Size: 10 192 743 794 20 4434
Fragments arranged by size:
4434 794 743 192 20 10
ClaI AT'CG_AT
Cuts at: 1554 2371 1554
Size: 817 5376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 3640 4529
Size: 54 1425 50 275 18 242 1568 889
Cuts at: 4529 6158 8
Size: 1629 43
Fragments arranged by size:
1629 1568 1425 889 275 242 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2072 3640 1537
Size: 275 260 1568 4090
Fragments arranged by size:
4090 1568 275 260
EarI CTCTTCn'nnn_
Cuts at: 1353 4123 1353
Size: 2770 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2600 2600
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2799 4256 4853 5640 5908 1239
Size: 393 1167 1457 597 787 268 1524
Fragments arranged by size:
1524 1457 1167 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2566 2566
Size: 6193
EcoO109I rG'GnC_Cy
Cuts at: 2085 2086 2721 3213 3363 2085
Size: 1 635 492 150 4915
Fragments arranged by size:
4915 635 492 150 1
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
EcoRV GAT'ATC
Cuts at: 3325 3325
Size: 6193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 6193
FseI GG_CCGG'CC
Cuts at: 2078 2078
Size: 6193
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 2051 5340 1678
Size: 373 3289 2531
Fragments arranged by size:
3289 2531 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2563 2595 2825 2857 3284
Size: 32 135 32 991 32 230 32 427
Cuts at: 3284 3316 3392 3424 4834 4866 4908 4940 1373
Size: 32 76 32 1410 32 42 32 2626
Fragments arranged by size:
2626 1410 991 427 230 135 76 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KasI G'GCGC_C
Cuts at: 2047 2047
Size: 6193
KpnI G_GTAC'C
Cuts at: 2221 3518 2221
Size: 1297 4896
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2188 2575 2845 3059 5307 5491 1305
Size: 883 387 270 214 2248 184 2007
Fragments arranged by size:
2248 2007 883 387 270 214 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3230 4151 4510 4669 518
Size: 648 2064 921 359 159 2042
Fragments arranged by size:
2064 2042 921 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2215 3609 4286 5434 5679 1606
Size: 609 1394 677 1148 245 2120
Fragments arranged by size:
2120 1394 1148 677 609 245
NaeI GCC'GGC
Cuts at: 2076 2076
Size: 6193
NarI GG'CG_CC
Cuts at: 2048 2048
Size: 6193
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
NdeI CA'TA_TG
Cuts at: 387 2586 3444 387
Size: 2199 858 3136
Fragments arranged by size:
3136 2199 858
NgoMIV G'CCGG_C
Cuts at: 2074 2074
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 2554 3142 3551 5096 1132
Size: 97 1325 588 409 1545 2229
Fragments arranged by size:
2229 1545 1325 588 409 97
PasI CC'CwG_GG
Cuts at: 1348 2052 1348
Size: 704 5489
PciI A'CATG_T
Cuts at: 3138 5092 3138
Size: 1954 4239
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PfoI T'CCnGG_A
Cuts at: 3218 3218
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2721 2721
Size: 6193
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 2085 3363 2085
Size: 1278 4915
PstI C_TGCA'G
Cuts at: 830 2979 3545 830
Size: 2149 566 3478
Fragments arranged by size:
3478 2149 566
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
SacI G_AGCT'C
Cuts at: 2602 2602
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2133 2673 2975 3541 4676 5357 5548
Size: 219 1088 540 302 566 1135 681 191
Cuts at: 5548 826
Size: 1471
Fragments arranged by size:
1471 1135 1088 681 566 540 302 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2084 2084
Size: 6193
SfoI GGC'GCC
Cuts at: 2049 2049
Size: 6193
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 4297 5198 5460 820
Size: 197 926 137 2217 901 262 1553
Fragments arranged by size:
2217 1553 926 901 262 197 137
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 2554 3551 1132
Size: 1422 997 3774
Fragments arranged by size:
3774 1422 997
SspI AAT'ATT
Cuts at: 5 52 2983 3040 4117 5
Size: 47 2931 57 1077 2081
Fragments arranged by size:
2931 2081 1077 57 47
StuI AGG'CCT
Cuts at: 1517 3092 1517
Size: 1575 4618
StyI C'CwwG_G
Cuts at: 513 1065 2004 3209 3396 3429 3632 513
Size: 552 939 1205 187 33 203 3074
Fragments arranged by size:
3074 1205 939 552 203 187 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2842 4220 4237 4390 4575 5020 325
Size: 1255 1262 1378 17 153 185 445 1498
Fragments arranged by size:
1498 1378 1262 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3119 4439 6151
Size: 274 80 33 51 1443 1142 1320 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
1712 1443 1320 1142 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3216 3394 3216
Size: 178 6015
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 2984 4322 2984
Size: 1338 4855
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII FspAI NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SrfI SwaI