Restriction map for
pFN21AE6418
(Circular) (Six-base) MAPSORT of: pFN21AE6418.seq Check: 6025 from: 1 to: 6574
pFN21AE6418 6574 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2668 2668
Size: 6574
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2499 3218 3917 1937
Size: 562 719 699 4594
Fragments arranged by size:
4594 719 699 562
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2674 2933 3344 3706 4637 6021 6289 1632
Size: 1042 259 411 362 931 1384 268 1917
Fragments arranged by size:
1917 1384 1042 931 411 362 268 259
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 5473 6450 1203
Size: 4270 977 1327
Fragments arranged by size:
4270 1327 977
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 3278 1166
Size: 2112 4462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AlwNI CAG_nnn'CTG
Cuts at: 4317 5889 4317
Size: 1572 5002
ApaI G_GGCC'C
Cuts at: 3297 3297
Size: 6574
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 3254 3750 3883 4109 4261 6370 1493
Size: 1761 496 133 226 152 2109 1697
Fragments arranged by size:
2109 1761 1697 496 226 152 133
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2448 3899 1870
Size: 73 271 234 1451 4545
Fragments arranged by size:
4545 1451 271 234 73
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3895 5350 618
Size: 325 217 627 2108 1455 1842
Fragments arranged by size:
2108 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2580 2731 3297 3542 3846 3893 1391
Size: 1189 151 566 245 304 47 4072
Fragments arranged by size:
4072 1189 566 304 245 151 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2954 3092 928
Size: 971 1055 138 4410
Fragments arranged by size:
4410 1055 971 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4765 4799 1903
Size: 34 50 34 2744 34 3678
Fragments arranged by size:
3678 2744 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 2935 1548
Size: 1387 5187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2612 3059 3068
Size: 107 122 71 631 605 940 447 9
Cuts at: 3068 3593 5185 136
Size: 525 1592 1525
Fragments arranged by size:
1592 1525 940 631 605 525 447 122
107 71 9
BglII A'GATC_T
Cuts at: 3430 6569 3430
Size: 3139 3435
BlpI GC'TnA_GC
Cuts at: 3991 3991
Size: 6574
Bme1580I G_kGCm'C
Cuts at: 1790 2415 3297 4635 5791 1790
Size: 625 882 1338 1156 2573
Fragments arranged by size:
2573 1338 1156 882 625
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2657 3001 3531
Size: 416 53 83 399 24 1240 344 530
Cuts at: 3531 4305 5263 442
Size: 774 958 1753
Fragments arranged by size:
1753 1240 958 774 530 416 399 344
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2540 2612 2722 2984 3357 3810 5234 1239
Size: 1301 72 110 262 373 453 1424 2579
Fragments arranged by size:
2579 1424 1301 453 373 262 110 72
Bpu10I CC'TnA_GC
Cuts at: 2421 2421
Size: 6574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3803 4699 5564 5862 3803
Size: 896 865 298 4515
Fragments arranged by size:
4515 896 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2144 3521 3647 5237 882
Size: 617 645 1377 126 1590 2219
Fragments arranged by size:
2219 1590 1377 645 617 126
BsaAI yAC'GTr
Cuts at: 493 1204 4365 493
Size: 711 3161 2702
Fragments arranged by size:
3161 2702 711
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 3663 4381 4763
Size: 53 83 186 936 696 1434 718 382
Cuts at: 4763 5398 275
Size: 635 1451
Fragments arranged by size:
1451 1434 936 718 696 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3546 5007 5405 5679 5826 6237 3546
Size: 1461 398 274 147 411 3883
Fragments arranged by size:
3883 1461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2088 2118 1178
Size: 30 165 30 685 30 5634
Fragments arranged by size:
5634 685 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 2981 3854 1186
Size: 1423 372 873 3906
Fragments arranged by size:
3906 1423 873 372
BseYI C'CCAG_C
Cuts at: 3846 5777 6273 3846
Size: 1931 496 4147
Fragments arranged by size:
4147 1931 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6001
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 2580 3816 4635 4720 5791 2427
Size: 153 1236 819 85 1071 3210
Fragments arranged by size:
3210 1236 1071 819 153 85
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
BsmBI CGTCTCn'nnnn_
Cuts at: 2851 2851
Size: 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2427 2580 2731 3297 3542 3816
Size: 399 625 12 153 151 566 245 274
Cuts at: 3816 3846 3893 4635 4720 5791 1391
Size: 30 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 566 399 274 245
153 151 85 47 30 12
BspEI T'CCGG_A
Cuts at: 3546 6237 3546
Size: 2691 3883
BspHI T'CATG_A
Cuts at: 2545 4463 6193 2545
Size: 1918 1730 2926
Fragments arranged by size:
2926 1918 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2668 3727 3929 844
Size: 1824 1059 202 3489
Fragments arranged by size:
3489 1824 1059 202
BsrBI CCG'CTC
Cuts at: 1394 1823 2509 2536 4461 1394
Size: 429 686 27 1925 3507
Fragments arranged by size:
3507 1925 686 429 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 3027 5069 5243 65
Size: 1183 1779 2042 174 1396
Fragments arranged by size:
2042 1779 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5218 5405 1700
Size: 110 3408 187 2869
Fragments arranged by size:
3408 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 3276 4634 5646 3276
Size: 1358 1012 4204
Fragments arranged by size:
4204 1358 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6574
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstXI CCAn_nnnn'nTGG
Cuts at: 2779 3643 3832 2779
Size: 864 189 5521
Fragments arranged by size:
5521 864 189
BstYI r'GATC_y
Cuts at: 1972 2634 3174 3430 3904 4271 4656 4673 6125
Size: 662 540 256 474 367 385 17 1452
Cuts at: 6125 6211 6223 6569 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 662 540 474 385 367 346
256 86 17 12
BstZ17I GTA'TAC
Cuts at: 3219 3219
Size: 6574
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 4090 4884 4904 3055
Size: 1035 794 20 4725
Fragments arranged by size:
4725 1035 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 2361 3878 2361
Size: 1517 5057
DraIII CAC_nnn'GTG
Cuts at: 3167 3783 3871 3167
Size: 616 88 5870
Fragments arranged by size:
5870 616 88
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 4021 4910
Size: 54 1425 50 275 18 189 2002 889
Cuts at: 4910 6539 8
Size: 1629 43
Fragments arranged by size:
2002 1629 1425 889 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2975 3324 3623 4504 1353
Size: 1622 349 299 881 3423
Fragments arranged by size:
3423 1622 881 349 299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2578 2578
Size: 6574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2540 2612 2674 2722 2933 2984 3344
Size: 393 908 72 62 48 211 51 360
Cuts at: 3344 3357 3706 3810 4637 5234 6021 6289 1239
Size: 13 349 104 827 597 787 268 1524
Fragments arranged by size:
1524 908 827 787 597 393 360 349
268 211 104 72 62 51 48 13
EcoO109I rG'GnC_Cy
Cuts at: 2625 3293 3861 2625
Size: 668 568 5338
Fragments arranged by size:
5338 668 568
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
EcoRV GAT'ATC
Cuts at: 3649 3649
Size: 6574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2851 2494
Size: 357 6217
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 2590 5721 1678
Size: 912 3131 2531
Fragments arranged by size:
3131 2531 912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2088 2120 3025 3057 3501
Size: 32 135 32 516 32 905 32 444
Cuts at: 3501 3533 5215 5247 5289 5321 1373
Size: 32 1682 32 42 32 2626
Fragments arranged by size:
2626 1682 905 516 444 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2500 3315 3918 4164 669
Size: 988 281 562 815 603 246 3079
Fragments arranged by size:
3079 988 815 603 562 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2784 5688 5872 1305
Size: 732 747 2904 184 2007
Fragments arranged by size:
2904 2007 747 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 10
Size: 54 1425 343 189 4563
Fragments arranged by size:
4563 1425 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2550 2894 3048 3194 3278 3378 4532
Size: 648 1384 344 154 146 84 100 1154
Cuts at: 4532 4891 5050 518
Size: 359 159 2042
Fragments arranged by size:
2042 1384 1154 648 359 344 159 154
146 100 84
MspA1I CmG'CkG
Cuts at: 1606 2068 3421 3990 4667 5815 6060 1606
Size: 462 1353 569 677 1148 245 2120
Fragments arranged by size:
2120 1353 1148 677 569 462 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6022
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2899 3616 3932 5477 1132
Size: 97 1670 717 316 1545 2229
Fragments arranged by size:
2229 1670 1545 717 316 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6574
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3167 3871 1108
Size: 2059 704 3811
Fragments arranged by size:
3811 2059 704
PfoI T'CCnGG_A
Cuts at: 2757 3621 2757
Size: 864 5710
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3861 3861
Size: 6574
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PspOMI G'GGCC_C
Cuts at: 3293 3293
Size: 6574
PspXI vC'TCGA_Gb
Cuts at: 2214 2214
Size: 6574
PstI C_TGCA'G
Cuts at: 830 3926 830
Size: 3096 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 3421 3421
Size: 6574
SacI G_AGCT'C
Cuts at: 2580 2580
Size: 6574
SalI G'TCGA_C
Cuts at: 1936 2498 3916 1936
Size: 562 1418 4594
Fragments arranged by size:
4594 1418 562
SapI GCTCTTCn'nnn_
Cuts at: 3623 3623
Size: 6574
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 2254 4822 6534 2254
Size: 2568 1712 2294
Fragments arranged by size:
2568 2294 1712
SfcI C'TryA_G
Cuts at: 826 1045 3092 3922 5057 5738 5929 826
Size: 219 2047 830 1135 681 191 1471
Fragments arranged by size:
2047 1471 1135 830 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3068 3593 3068
Size: 525 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3901 1872
Size: 2029 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2214 2448 3818 4678 5579 5841
Size: 197 926 271 234 1370 860 901 262
Cuts at: 5841 820
Size: 1553
Fragments arranged by size:
1553 1370 926 901 860 271 262 234
197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 2175 4013 513
Size: 552 1110 1838 3074
Fragments arranged by size:
3074 1838 1110 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4601 4618 4771 4956 5401 325
Size: 1255 3021 17 153 185 445 1498
Fragments arranged by size:
3021 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2252 3447 4820
Size: 274 80 33 51 1443 275 1195 1373
Cuts at: 4820 6532 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1373 1195 275 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 3806 3838 6534 6566 2543
Size: 32 1231 32 2696 32 2551
Fragments arranged by size:
2696 2551 1231 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3145 1316
Size: 1829 4745
XbaI T'CTAG_A
Cuts at: 3250 3910 3250
Size: 660 5914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3640 3664 3640
Size: 24 6550
XhoI C'TCGA_G
Cuts at: 1943 2214 2448 1943
Size: 271 234 6069
Fragments arranged by size:
6069 271 234
XmaI C'CCGG_G
Cuts at: 1870 3899 1870
Size: 2029 4545
XmnI GAAnn'nnTTC
Cuts at: 4703 4703
Size: 6574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SacII SanDI SexAI SfoI SrfI SwaI