Restriction map for
pFN21AE6417
(Circular) (Six-base) MAPSORT of: pFN21AE6417.seq Check: 1135 from: 1 to: 6385
pFN21AE6417 6385 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3590 3590
Size: 6385
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3728 1937
Size: 1791 4594
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 4448 5832 6100 1632
Size: 575 2241 1384 268 1917
Fragments arranged by size:
2241 1917 1384 575 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 2040 2439 5284 6261 1203
Size: 837 399 2845 977 1327
Fragments arranged by size:
2845 1327 977 837 399
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 5114 5114
Size: 6385
AleI CACnn'nnGTG
Cuts at: 1166 2903 3311 1166
Size: 1737 408 4240
Fragments arranged by size:
4240 1737 408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2684 1184
Size: 1500 4885
AlwNI CAG_nnn'CTG
Cuts at: 2751 3007 3242 4128 5700 2751
Size: 256 235 886 1572 3436
Fragments arranged by size:
3436 1572 886 256 235
ApaI G_GGCC'C
Cuts at: 2737 3578 2737
Size: 841 5544
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 3694 3920 4072 6181 1493
Size: 2201 226 152 2109 1697
Fragments arranged by size:
2201 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 3450 3710 1870
Size: 73 678 829 260 4545
Fragments arranged by size:
4545 829 678 260 73
BamHI G'GATC_C
Cuts at: 2547 3715 4082 2547
Size: 1168 367 4850
Fragments arranged by size:
4850 1168 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2124 2521 2648 3122
Size: 325 217 627 221 116 397 127 474
Cuts at: 3122 3461 3706 5161 618
Size: 339 245 1455 1842
Fragments arranged by size:
1842 1455 627 474 397 339 325 245
221 217 127 116
BanII G_rGCy'C
Cuts at: 1391 2172 2408 2737 2931 3364 3567 3578 3704
Size: 781 236 329 194 433 203 11 126
Cuts at: 3704 1391
Size: 4072
Fragments arranged by size:
4072 781 433 329 236 203 194 126
11
BbeI G_GCGC'C
Cuts at: 2128 2128
Size: 6385
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2674 2974 928
Size: 971 775 300 4339
Fragments arranged by size:
4339 971 775 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4576 4610 1903
Size: 34 2639 34 3678
Fragments arranged by size:
3678 2639 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6385
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2024 2103 2268
Size: 107 122 71 631 605 352 79 165
Cuts at: 2268 4996 136
Size: 2728 1525
Fragments arranged by size:
2728 1525 631 605 352 165 122 107
79 71
BglII A'GATC_T
Cuts at: 6380 6380
Size: 6385
BlpI GC'TnA_GC
Cuts at: 2407 3037 3802 2407
Size: 630 765 4990
Fragments arranged by size:
4990 765 630
Bme1580I G_kGCm'C
Cuts at: 1790 2651 2737 3578 4446 5602 1790
Size: 861 86 841 868 1156 2573
Fragments arranged by size:
2573 1156 868 861 841 86
BmgBI CAC'GTC
Cuts at: 1318 2041 3595 1318
Size: 723 1554 4108
Fragments arranged by size:
4108 1554 723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2492 3367 4116
Size: 416 53 83 399 24 1075 875 749
Cuts at: 4116 5074 442
Size: 958 1753
Fragments arranged by size:
1753 1075 958 875 749 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 6385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2184 2200 2867 3016 3092 3488 3692 5045
Size: 945 16 667 149 76 396 204 1353
Cuts at: 5045 1239
Size: 2579
Fragments arranged by size:
2579 1353 945 667 396 204 149 76
16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2134 4510 5375 5673 2134
Size: 2376 865 298 2846
Fragments arranged by size:
2846 2376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2218 2341 2366 3479 3668 5048 882
Size: 617 719 123 25 1113 189 1380 2219
Fragments arranged by size:
2219 1380 1113 719 617 189 123 25
BsaAI yAC'GTr
Cuts at: 493 1204 4176 493
Size: 711 2972 2702
Fragments arranged by size:
2972 2702 711
BsaBI GATnn'nnATC
Cuts at: 2195 2552 6379 2195
Size: 357 3827 2201
Fragments arranged by size:
3827 2201 357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2125 2271 4192 4574
Size: 53 83 186 936 592 146 1921 382
Cuts at: 4574 5209 275
Size: 635 1451
Fragments arranged by size:
1921 1451 936 635 592 382 186 146
83 53
BsaWI w'CCGG_w
Cuts at: 2228 2433 4818 5216 5490 5637 6048 2228
Size: 205 2385 398 274 147 411 2565
Fragments arranged by size:
2565 2385 411 398 274 205 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6160
Fragments arranged by size:
6160 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2090 2529 2543 2585 3019 3635 1186
Size: 865 39 439 14 42 434 616 3936
Fragments arranged by size:
3936 865 616 439 434 42 39 14
BseYI C'CCAG_C
Cuts at: 2446 2671 2710 2881 3394 3522 5588 6084 2446
Size: 225 39 171 513 128 2066 496 2747
Fragments arranged by size:
2747 2066 513 496 225 171 128 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2609 3634 1163
Size: 573 329 544 1025 3914
Fragments arranged by size:
3914 1025 573 544 329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3835 4596 5624 1540
Size: 275 186 1090 744 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 744 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2408 2922 3306 3567 4446 4531 5602 2172
Size: 236 514 384 261 879 85 1071 2955
Fragments arranged by size:
2955 1071 879 514 384 261 236 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2408 2651 2737 2922 2931 3306
Size: 399 382 236 243 86 185 9 375
Cuts at: 3306 3364 3567 3578 3704 4446 4531 5602 1391
Size: 58 203 11 126 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 375 243 236
203 185 126 86 85 58 11 9
BspEI T'CCGG_A
Cuts at: 2228 2433 6048 2228
Size: 205 3615 2565
Fragments arranged by size:
3615 2565 205
BspHI T'CATG_A
Cuts at: 2400 2775 4274 6004 2400
Size: 375 1499 1730 2781
Fragments arranged by size:
2781 1730 1499 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3590 3740 844
Size: 2746 150 3489
Fragments arranged by size:
3489 2746 150
BsrBI CCG'CTC
Cuts at: 1394 1823 3613 4272 1394
Size: 429 1790 659 3507
Fragments arranged by size:
3507 1790 659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2829 3345 4880 5054 65
Size: 1183 1581 516 1535 174 1396
Fragments arranged by size:
1581 1535 1396 1183 516 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3090 3278 5029 5216 1700
Size: 110 1280 188 1751 187 2869
Fragments arranged by size:
2869 1751 1280 188 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6385
BssSI C'ACGA_G
Cuts at: 4445 5457 4445
Size: 1012 5373
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3007 794
Size: 2213 4172
BstBI TT'CG_AA
Cuts at: 5189 5189
Size: 6385
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6385
BstXI CCAn_nnnn'nTGG
Cuts at: 2162 2308 3136 3530 2162
Size: 146 828 394 5017
Fragments arranged by size:
5017 828 394 146
BstYI r'GATC_y
Cuts at: 1972 2547 2841 3715 4082 4467 4484 5936 6022
Size: 575 294 874 367 385 17 1452 86
Cuts at: 6022 6034 6380 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 874 575 385 367 346 294
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2280 2376 2697 2901 2923 2989
Size: 552 939 276 96 321 204 22 66
Cuts at: 2989 3117 3291 3312 513
Size: 128 174 21 3586
Fragments arranged by size:
3586 939 552 321 276 204 174 128
96 66 22 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2670 2998 3082 3163 487
Size: 683 56 1444 328 84 81 3709
Fragments arranged by size:
3709 1444 683 328 84 81 56
BtsI GCAGTG_nn'
Cuts at: 3044 3144 3240 3646 3901 4695 4715 3044
Size: 100 96 406 255 794 20 4714
Fragments arranged by size:
4714 794 406 255 100 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2482 2517 529
Size: 35 1918 35 4397
Fragments arranged by size:
4397 1918 35 35
DraI TTT'AAA
Cuts at: 3689 3689
Size: 6385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5392 809
Size: 507 4076 1802
Fragments arranged by size:
4076 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3021 3165 3211
Size: 54 1425 50 275 18 1191 144 46
Cuts at: 3211 3832 4721 6350 8
Size: 621 889 1629 43
Fragments arranged by size:
1629 1425 1191 889 621 275 144 54
50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3536 4315 1353
Size: 2183 779 3423
Fragments arranged by size:
3423 2183 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 2560 4972 5346 5492 387
Size: 1064 138 476 495 2412 374 146 1280
Fragments arranged by size:
2412 1280 1064 495 476 374 146 138
EcoICRI GAG'CTC
Cuts at: 2170 2406 3565 2170
Size: 236 1159 4990
Fragments arranged by size:
4990 1159 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2184 2200 2207 2867 3016 3092 3488
Size: 393 552 16 7 660 149 76 396
Cuts at: 3488 3692 4448 5045 5832 6100 1239
Size: 204 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 660 597 552 396 393
268 204 149 76 16 7
EcoO109I rG'GnC_Cy
Cuts at: 2118 2533 2733 2734 3575 2118
Size: 415 200 1 841 4928
Fragments arranged by size:
4928 841 415 200 1
EcoRI G'AATT_C
Cuts at: 3694 4072 3694
Size: 378 6007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 6385
FseI GG_CCGG'CC
Cuts at: 3282 3282
Size: 6385
FspI TGC'GCA
Cuts at: 4891 4891
Size: 6385
HaeII r_GCGC'y
Cuts at: 1678 2128 5532 1678
Size: 450 3404 2531
Fragments arranged by size:
3404 2531 450
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 5026 5058 5100
Size: 32 135 32 477 32 2945 32 42
Cuts at: 5100 5132 1373
Size: 32 2626
Fragments arranged by size:
2945 2626 477 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3190 3355 3729 3975 669
Size: 988 281 1252 165 374 246 3079
Fragments arranged by size:
3079 1252 988 374 281 246 165
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6385
HpaI GTT'AAC
Cuts at: 3975 3975
Size: 6385
KasI G'GCGC_C
Cuts at: 2124 2124
Size: 6385
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 3416 5499 5683 1305
Size: 1098 1013 2083 184 2007
Fragments arranged by size:
2083 2007 1098 1013 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3023 3213 10
Size: 54 1425 343 1191 190 3182
Fragments arranged by size:
3182 1425 1191 343 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2330 2666 2903 3134 3311 4343
Size: 648 1140 24 336 237 231 177 1032
Cuts at: 4343 4702 4861 518
Size: 359 159 2042
Fragments arranged by size:
2042 1140 1032 648 359 336 237 231
177 159 24
MspA1I CmG'CkG
Cuts at: 1606 2730 2991 3263 3413 3632 3801 4478 5626
Size: 1124 261 272 150 219 169 677 1148
Cuts at: 5626 5871 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1124 677 272 261 245 219
169 150
NaeI GCC'GGC
Cuts at: 3280 3280
Size: 6385
NarI GG'CG_CC
Cuts at: 2125 2125
Size: 6385
NcoI C'CATG_G
Cuts at: 513 1065 2004 2697 3291 513
Size: 552 939 693 594 3607
Fragments arranged by size:
3607 939 693 594 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NgoMIV G'CCGG_C
Cuts at: 3278 3278
Size: 6385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 2018 2443 3743 5288 1132
Size: 97 789 425 1300 1545 2229
Fragments arranged by size:
2229 1545 1300 789 425 97
PasI CC'CwG_GG
Cuts at: 1348 2862 3571 1348
Size: 1514 709 4162
Fragments arranged by size:
4162 1514 709
PciI A'CATG_T
Cuts at: 2439 5284 2439
Size: 2845 3540
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6385
PfoI T'CCnGG_A
Cuts at: 2565 2844 3504 2565
Size: 279 660 5446
Fragments arranged by size:
5446 660 279
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 5967 5999 4454
Size: 32 1481 32 4840
Fragments arranged by size:
4840 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2118 2118
Size: 6385
PshAI GACnn'nnGTC
Cuts at: 1613 3680 1613
Size: 2067 4318
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PspOMI G'GGCC_C
Cuts at: 2733 3574 2733
Size: 841 5544
PstI C_TGCA'G
Cuts at: 830 3003 3247 3737 830
Size: 2173 244 490 3478
Fragments arranged by size:
3478 2173 490 244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 3263 3263
Size: 6385
SacI G_AGCT'C
Cuts at: 2172 2408 3567 2172
Size: 236 1159 4990
Fragments arranged by size:
4990 1159 236
SacII CC_GC'GG
Cuts at: 2992 2992
Size: 6385
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SbfI CC_TGCA'GG
Cuts at: 3003 3737 3003
Size: 734 5651
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SfcI C'TryA_G
Cuts at: 826 1045 2999 3243 3733 4868 5549 5740 826
Size: 219 1954 244 490 1135 681 191 1471
Fragments arranged by size:
1954 1471 1135 681 490 244 219 191
SfoI GGC'GCC
Cuts at: 2126 2126
Size: 6385
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6385
SmaI CCC'GGG
Cuts at: 1872 3452 3712 1872
Size: 1580 260 4545
Fragments arranged by size:
4545 1580 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2113 4489 5390 5652 820
Size: 197 926 170 2376 901 262 1553
Fragments arranged by size:
2376 1553 926 901 262 197 170
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 2018 3743 1132
Size: 886 1725 3774
Fragments arranged by size:
3774 1725 886
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 3557 1517
Size: 2040 4345
StyI C'CwwG_G
Cuts at: 513 1065 2004 2514 2697 3291 3824 513
Size: 552 939 510 183 594 533 3074
Fragments arranged by size:
3074 939 594 552 533 510 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2364 2711 2761 3110 3386 4412 4429
Size: 1255 784 347 50 349 276 1026 17
Cuts at: 4429 4582 4767 5212 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1026 784 445 349 347 276
185 153 50 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4631 6343 96
Size: 274 80 33 51 1443 2654 1712 138
Fragments arranged by size:
2654 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6345 6377 6345
Size: 32 6353
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6385
XbaI T'CTAG_A
Cuts at: 3721 3721
Size: 6385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 3133 2573
Size: 560 5825
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6385
XmaI C'CCGG_G
Cuts at: 1870 3450 3710 1870
Size: 1580 260 4545
Fragments arranged by size:
4545 1580 260
XmnI GAAnn'nnTTC
Cuts at: 2441 4514 2441
Size: 2073 4312
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SrfI SwaI