Restriction map for
pFN21AE6416
(Circular) (Six-base) MAPSORT of: pFN21AE6416.seq Check: 31 from: 1 to: 6352
pFN21AE6416 6352 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2400 3695 1937
Size: 463 1295 4594
Fragments arranged by size:
4594 1295 463
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2323 2344 4415 5799 6067 1632
Size: 608 83 21 2071 1384 268 1917
Fragments arranged by size:
2071 1917 1384 608 268 83 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6155
AflIII A'CryG_T
Cuts at: 1203 5251 6228 1203
Size: 4048 977 1327
Fragments arranged by size:
4048 1327 977
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 2486 1166
Size: 1320 5032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2246 1184
Size: 1062 5290
AlwNI CAG_nnn'CTG
Cuts at: 2990 4095 5667 2990
Size: 1105 1572 3675
Fragments arranged by size:
3675 1572 1105
ApaI G_GGCC'C
Cuts at: 2383 2383
Size: 6352
ApaLI G'TGCA_C
Cuts at: 4409 5565 4409
Size: 1156 5196
ApoI r'AATT_y
Cuts at: 1493 2056 2564 3596 3661 3887 4039 6148 1493
Size: 563 508 1032 65 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 563 508 226 152 65
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 1870
Size: 73 1734 4545
Fragments arranged by size:
4545 1734 73
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3673 5128 618
Size: 325 217 627 1886 1455 1842
Fragments arranged by size:
1886 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2383 3671 1391
Size: 992 1288 4072
Fragments arranged by size:
4072 1288 992
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2516 2550 4543 4577 1903
Size: 34 579 34 1993 34 3678
Fragments arranged by size:
3678 1993 579 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2161 4244 5454 2161
Size: 2083 1210 3059
Fragments arranged by size:
3059 2083 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3047 4963 136
Size: 107 122 71 631 605 1375 1916 1525
Fragments arranged by size:
1916 1525 1375 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6347 6347
Size: 6352
BlpI GC'TnA_GC
Cuts at: 3769 3769
Size: 6352
Bme1580I G_kGCm'C
Cuts at: 1790 2383 4413 5569 1790
Size: 593 2030 1156 2573
Fragments arranged by size:
2573 2030 1156 593
BmgBI CAC'GTC
Cuts at: 1318 2530 1318
Size: 1212 5140
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3509 4083 5041
Size: 416 53 83 399 24 2092 574 958
Cuts at: 5041 442
Size: 1753
Fragments arranged by size:
2092 1753 958 574 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5012 1239
Size: 3773 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4477 5342 5640 4477
Size: 865 298 5189
Fragments arranged by size:
5189 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5015 882
Size: 617 3516 2219
Fragments arranged by size:
3516 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2251 4143 493
Size: 711 1047 1892 2702
Fragments arranged by size:
2702 1892 1047 711
BsaBI GATnn'nnATC
Cuts at: 6346 6346
Size: 6352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4159 4541 5176 275
Size: 53 83 186 936 2626 382 635 1451
Fragments arranged by size:
2626 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4785 5183 5457 5604 6015 4785
Size: 398 274 147 411 5122
Fragments arranged by size:
5122 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6127
Fragments arranged by size:
6127 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6352
BseYI C'CCAG_C
Cuts at: 3114 5555 6051 3114
Size: 2441 496 3415
Fragments arranged by size:
3415 2441 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5779
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2419 3802 4563 5591 1540
Size: 275 186 418 1383 761 1028 2301
Fragments arranged by size:
2301 1383 1028 761 418 275 186
BsiHKAI G_wGCw'C
Cuts at: 4413 4498 5569 4413
Size: 85 1071 5196
Fragments arranged by size:
5196 1071 85
BsmI GAATG_Cn'
Cuts at: 3863 3956 3863
Size: 93 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2383 3671 4413 4498 5569 1391
Size: 399 593 1288 742 85 1071 2174
Fragments arranged by size:
2174 1288 1071 742 593 399 85
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 2466 4241 5971 2466
Size: 1775 1730 2847
Fragments arranged by size:
2847 1775 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 3707 844
Size: 2254 609 3489
Fragments arranged by size:
3489 2254 609
BsrBI CCG'CTC
Cuts at: 1394 1823 2085 4239 1394
Size: 429 262 2154 3507
Fragments arranged by size:
3507 2154 429 262
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 2695 4847 5021 65
Size: 1183 1338 109 2152 174 1396
Fragments arranged by size:
2152 1396 1338 1183 174 109
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2171 4996 5183 1700
Size: 110 227 134 2825 187 2869
Fragments arranged by size:
2869 2825 227 187 134 110
BsrGI T'GTAC_A
Cuts at: 96 3279 96
Size: 3183 3169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2987 794
Size: 2193 4159
BstBI TT'CG_AA
Cuts at: 5156 5156
Size: 6352
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstXI CCAn_nnnn'nTGG
Cuts at: 2106 2488 2106
Size: 382 5970
BstYI r'GATC_y
Cuts at: 1972 3418 3682 4049 4434 4451 5903 5989 6001
Size: 1446 264 367 385 17 1452 86 12
Cuts at: 6001 6347 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1446 385 367 346 264 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5800
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5613
Fragments arranged by size:
5613 683 56
BtsI GCAGTG_nn'
Cuts at: 3102 3868 4662 4682 3102
Size: 766 794 20 4772
Fragments arranged by size:
4772 794 766 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2280 2315 529
Size: 35 1716 35 4566
Fragments arranged by size:
4566 1716 35 35
DraI TTT'AAA
Cuts at: 3360 3601 3656 3360
Size: 241 55 6056
Fragments arranged by size:
6056 241 55
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2591 5359 809
Size: 507 1275 2768 1802
Fragments arranged by size:
2768 1802 1275 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 2571 3799
Size: 54 1425 50 275 18 218 523 1228
Cuts at: 3799 4688 6317 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1228 889 523 275 218 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2163 2468 3009 4282 1353
Size: 722 88 305 541 1273 3423
Fragments arranged by size:
3423 1273 722 541 305 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2323 2344 4415 5012 5799 6067
Size: 393 608 83 21 2071 597 787 268
Cuts at: 6067 1239
Size: 1524
Fragments arranged by size:
2071 1524 787 608 597 393 268 83
21
EcoO109I rG'GnC_Cy
Cuts at: 2380 3110 2380
Size: 730 5622
EcoRI G'AATT_C
Cuts at: 2564 3661 4039 2564
Size: 1097 378 4877
Fragments arranged by size:
4877 1097 378
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 5499 1678
Size: 3821 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3605 3637 4993 5025 5067
Size: 32 135 32 2033 32 1356 32 42
Cuts at: 5067 5099 1373
Size: 32 2626
Fragments arranged by size:
2626 2033 1356 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2610 3696 3942 669
Size: 988 281 672 1086 246 3079
Fragments arranged by size:
3079 1086 988 672 281 246
HindIII A'AGCT_T
Cuts at: 748 2331 748
Size: 1583 4769
HpaI GTT'AAC
Cuts at: 2610 3942 2610
Size: 1332 5020
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5466 5650 1305
Size: 4161 184 2007
Fragments arranged by size:
4161 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4530
Fragments arranged by size:
4530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3628 4310 4669 4828 518
Size: 648 1320 1142 682 359 159 2042
Fragments arranged by size:
2042 1320 1142 682 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2132 2672 3768 4445 5593 5838 1606
Size: 526 540 1096 677 1148 245 2120
Fragments arranged by size:
2120 1148 1096 677 540 526 245
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 6352
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5800
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6352
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 6352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 3289 3710 5255 1132
Size: 97 2060 421 1545 2229
Fragments arranged by size:
2229 2060 1545 421 97
PasI CC'CwG_GG
Cuts at: 1348 2269 1348
Size: 921 5431
PciI A'CATG_T
Cuts at: 5251 5251
Size: 6352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3335 1108
Size: 2227 4125
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3110 3110
Size: 6352
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 2918 3922 2918
Size: 1004 5348
PspOMI G'GGCC_C
Cuts at: 2379 2379
Size: 6352
PstI C_TGCA'G
Cuts at: 830 3109 3704 830
Size: 2279 595 3478
Fragments arranged by size:
3478 2279 595
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SapI GCTCTTCn'nnn_
Cuts at: 2075 2163 2075
Size: 88 6264
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SfcI C'TryA_G
Cuts at: 826 1045 3105 3148 3396 3700 4835 5516 5707
Size: 219 2060 43 248 304 1135 681 191
Cuts at: 5707 826
Size: 1471
Fragments arranged by size:
2060 1471 1135 681 304 248 219 191
43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4456 5357 5619 820
Size: 197 926 2513 901 262 1553
Fragments arranged by size:
2513 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3710 1132
Size: 2578 3774
SspI AAT'ATT
Cuts at: 5 52 2735 4276 5
Size: 47 2683 1541 2081
Fragments arranged by size:
2683 2081 1541 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6352
StyI C'CwwG_G
Cuts at: 513 1065 3791 513
Size: 552 2726 3074
Fragments arranged by size:
3074 2726 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2405 3491 4379 4396 4549 4734
Size: 1255 446 379 1086 888 17 153 185
Cuts at: 4734 5179 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1086 888 446 445 379 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3279 4598 6310
Size: 274 80 33 51 1443 1302 1319 1712
Cuts at: 6310 96
Size: 138
Fragments arranged by size:
1712 1443 1319 1302 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2190 2222 3094 3126 6312 6344 2190
Size: 32 872 32 3186 32 2198
Fragments arranged by size:
3186 2198 872 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2629 2629
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 2157 4481 2157
Size: 2324 4028
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI