Restriction map for
pFN21AE6414
(Circular) (Six-base) MAPSORT of: pFN21AE6414.seq Check: 3287 from: 1 to: 6139
pFN21AE6414 6139 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2554 3482 1937
Size: 617 928 4594
Fragments arranged by size:
4594 928 617
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2938 4202 5586 5854 1632
Size: 1306 1264 1384 268 1917
Fragments arranged by size:
1917 1384 1306 1264 268
AflII C'TTAA_G
Cuts at: 820 1017 2476 820
Size: 197 1459 4483
Fragments arranged by size:
4483 1459 197
AflIII A'CryG_T
Cuts at: 1203 3219 3225 5038 6015 1203
Size: 2016 6 1813 977 1327
Fragments arranged by size:
2016 1813 1327 977 6
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 3143 1166
Size: 1977 4162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 3882 5454 3882
Size: 1572 4567
ApaLI G'TGCA_C
Cuts at: 3105 4196 5352 3105
Size: 1091 1156 3892
Fragments arranged by size:
3892 1156 1091
ApoI r'AATT_y
Cuts at: 1493 2142 2940 3448 3674 3826 5935 1493
Size: 649 798 508 226 152 2109 1697
Fragments arranged by size:
2109 1697 798 649 508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2909 4693 160
Size: 872 1877 1784 1606
Fragments arranged by size:
1877 1784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 3464 1870
Size: 73 1521 4545
Fragments arranged by size:
4545 1521 73
AvrII C'CTAG_G
Cuts at: 2779 2779
Size: 6139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2324 2357 2324
Size: 33 6106
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3460 4915 618
Size: 325 217 627 1673 1455 1842
Fragments arranged by size:
1842 1673 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3458 1391
Size: 2067 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3341 928
Size: 971 1442 3726
Fragments arranged by size:
3726 1442 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4330 4364 1903
Size: 34 50 34 2309 34 3678
Fragments arranged by size:
3678 2309 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BfrBI ATG'CAT
Cuts at: 2108 2108
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 4750 136
Size: 107 122 71 631 605 901 2177 1525
Fragments arranged by size:
2177 1525 901 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2999 3109 4200 5356 1790
Size: 236 973 110 1091 1156 2573
Fragments arranged by size:
2573 1156 1091 973 236 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3152 3870 4828
Size: 416 53 83 399 24 1735 718 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1753 1735 958 718 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2028 3225 4799 1239
Size: 789 1197 1574 2579
Fragments arranged by size:
2579 1574 1197 789
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3376 4264 5129 5427 3376
Size: 888 865 298 4088
Fragments arranged by size:
4088 888 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2584 4802 882
Size: 617 1085 2218 2219
Fragments arranged by size:
2219 2218 1085 617
BsaAI yAC'GTr
Cuts at: 493 1204 3930 493
Size: 711 2726 2702
Fragments arranged by size:
2726 2702 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2211 2241 1178
Size: 30 165 30 808 30 5076
Fragments arranged by size:
5076 808 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3165 1186
Size: 1979 4160
BseYI C'CCAG_C
Cuts at: 5342 5838 5342
Size: 496 5643
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2264 3589 4350 5378 1540
Size: 275 186 263 1325 761 1028 2301
Fragments arranged by size:
2301 1325 1028 761 275 263 186
BsiHKAI G_wGCw'C
Cuts at: 3109 4200 4285 5356 3109
Size: 1091 85 1071 3892
Fragments arranged by size:
3892 1091 1071 85
BsmI GAATG_Cn'
Cuts at: 2108 2569 3650 3743 2108
Size: 461 1081 93 4504
Fragments arranged by size:
4504 1081 461 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2999 3109 3458 4200 4285 5356
Size: 399 236 973 110 349 742 85 1071
Cuts at: 5356 1391
Size: 2174
Fragments arranged by size:
2174 1071 973 742 399 349 236 110
85
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 2586 4028 5758 2586
Size: 1442 1730 2967
Fragments arranged by size:
2967 1730 1442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2394 3173 3494 844
Size: 1550 779 321 3489
Fragments arranged by size:
3489 1550 779 321
BsrBI CCG'CTC
Cuts at: 1394 1823 4026 1394
Size: 429 2203 3507
Fragments arranged by size:
3507 2203 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3283 4634 4808 65
Size: 1183 2035 1351 174 1396
Fragments arranged by size:
2035 1396 1351 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 2263 4783 4970 1700
Size: 110 449 4 2520 187 2869
Fragments arranged by size:
2869 2520 449 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 3222 96
Size: 3126 3013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 3098 4199 5211 3098
Size: 1101 1012 4026
Fragments arranged by size:
4026 1101 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2200
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134
Size: 1497 367 385 17 1452 86 12 346
Cuts at: 6134 1972
Size: 1977
Fragments arranged by size:
1977 1497 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2436 2454 513
Size: 552 1371 18 4198
Fragments arranged by size:
4198 1371 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5400
Fragments arranged by size:
5400 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3655 4449 4469 3076
Size: 579 794 20 4746
Fragments arranged by size:
4746 794 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DraIII CAC_nnn'GTG
Cuts at: 3410 3410
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2261 2451 3586
Size: 54 1425 50 275 18 431 190 1135
Cuts at: 3586 4475 6104 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 431 275 190 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2261 3586 1537
Size: 275 449 1325 4090
Fragments arranged by size:
4090 1325 449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4069 1353
Size: 2716 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2938 3225 4202 4799 5586 5854
Size: 393 396 910 287 977 597 787 268
Cuts at: 5854 1239
Size: 1524
Fragments arranged by size:
1524 977 910 787 597 396 393 287
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2901 3180 2901
Size: 279 5860
EcoO109I rG'GnC_Cy
Cuts at: 2222 2222
Size: 6139
EcoRI G'AATT_C
Cuts at: 2940 3448 3826 2940
Size: 508 378 5253
Fragments arranged by size:
5253 508 378
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 5286 1678
Size: 3608 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 4780 4812 4854
Size: 32 135 32 1256 32 1920 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 1920 1256 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3385 3483 3729 669
Size: 988 281 1447 98 246 3079
Fragments arranged by size:
3079 1447 988 281 246 98
HindIII A'AGCT_T
Cuts at: 748 2533 748
Size: 1785 4354
HpaI GTT'AAC
Cuts at: 3385 3729 3385
Size: 344 5795
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 3141 3738 3141
Size: 597 5542
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2042 2053 3109 5253 5437 1305
Size: 737 11 1056 2144 184 2007
Fragments arranged by size:
2144 2007 1056 737 184 11
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 10
Size: 54 1425 343 621 3696
Fragments arranged by size:
3696 1425 621 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3143 3224 4097 4456 4615 518
Size: 648 1977 81 873 359 159 2042
Fragments arranged by size:
2042 1977 873 648 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 2338 3555 4232 5380 5625 1606
Size: 732 1217 677 1148 245 2120
Fragments arranged by size:
2120 1217 1148 732 677 245
NaeI GCC'GGC
Cuts at: 2261 2261
Size: 6139
NcoI C'CATG_G
Cuts at: 513 1065 2436 2454 513
Size: 552 1371 18 4198
Fragments arranged by size:
4198 1371 552 18
NdeI CA'TA_TG
Cuts at: 387 2815 387
Size: 2428 3711
NgoMIV G'CCGG_C
Cuts at: 2259 2259
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NsiI A_TGCA'T
Cuts at: 2110 2110
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 3223 3229 3497 5042 1132
Size: 97 1994 6 268 1545 2229
Fragments arranged by size:
2229 1994 1545 268 97 6
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 3219 3225 5038 3219
Size: 6 1813 4320
Fragments arranged by size:
4320 1813 6
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2222
Size: 6139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PstI C_TGCA'G
Cuts at: 830 3491 830
Size: 2661 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2338 2338
Size: 6139
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 2843 2948 4387 6099 2843
Size: 105 1439 1712 2883
Fragments arranged by size:
2883 1712 1439 105
SfcI C'TryA_G
Cuts at: 826 1045 2167 2422 2901 3289 3487 4622 5303
Size: 219 1122 255 479 388 198 1135 681
Cuts at: 5303 5494 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1122 681 479 388 255 219
198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 3466 1872
Size: 1594 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2476 3355 4243 5144 5406 820
Size: 197 926 533 879 888 901 262 1553
Fragments arranged by size:
1553 926 901 888 879 533 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 2154 4063 5
Size: 47 2102 1909 2081
Fragments arranged by size:
2102 2081 1909 47
StuI AGG'CCT
Cuts at: 1517 2896 1517
Size: 1379 4760
StyI C'CwwG_G
Cuts at: 513 1065 2436 2454 2596 2779 2827 3049 3578
Size: 552 1371 18 142 183 48 222 529
Cuts at: 3578 513
Size: 3074
Fragments arranged by size:
3074 1371 552 529 222 183 142 48
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3092 4166 4183 4336 4521 4966 325
Size: 1255 1512 1074 17 153 185 445 1498
Fragments arranged by size:
1512 1498 1255 1074 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 2946 3222
Size: 274 80 33 51 1443 864 105 276
Cuts at: 3222 4385 6097 96
Size: 1163 1712 138
Fragments arranged by size:
1712 1443 1163 864 276 274 138 105
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2052 2803 2052
Size: 751 5388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 3464 1870
Size: 1594 4545
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI