Restriction map for
pFN21AE6413
(Circular) (Six-base) MAPSORT of: pFN21AE6413.seq Check: 5438 from: 1 to: 6037
pFN21AE6413 6037 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3847 278
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2763 3380 1937
Size: 826 617 4594
Fragments arranged by size:
4594 826 617
Acc65I G'GTAC_C
Cuts at: 2059 3358 2059
Size: 1299 4738
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2423 4100 5484 5752 1632
Size: 791 1677 1384 268 1917
Fragments arranged by size:
1917 1677 1384 791 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 4936 5913 1203
Size: 3733 977 1327
Fragments arranged by size:
3733 1327 977
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 2050 1166
Size: 884 5153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2144 2244 1184
Size: 960 100 4977
Fragments arranged by size:
4977 960 100
AlwNI CAG_nnn'CTG
Cuts at: 2401 3122 3780 5352 2401
Size: 721 658 1572 3086
Fragments arranged by size:
3086 1572 721 658
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 2584 2652 2848 3346 3572 3724 5833 1493
Size: 1091 68 196 498 226 152 2109 1697
Fragments arranged by size:
2109 1697 1091 498 226 196 152 68
AseI AT'TA_AT
Cuts at: 160 1032 2676 2693 4591 160
Size: 872 1644 17 1898 1606
Fragments arranged by size:
1898 1644 1606 872 17
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 2643 3362 1870
Size: 73 474 226 719 4545
Fragments arranged by size:
4545 719 474 226 73
BamHI G'GATC_C
Cuts at: 3367 3734 3367
Size: 367 5670
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2059 2800 3358 4813
Size: 325 217 627 227 45 741 558 1455
Cuts at: 4813 618
Size: 1842
Fragments arranged by size:
1842 1455 741 627 558 325 227 217
45
BanII G_rGCy'C
Cuts at: 1391 2388 2424 3356 1391
Size: 997 36 932 4072
Fragments arranged by size:
4072 997 932 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2293 2743 2949 928
Size: 971 394 450 206 4016
Fragments arranged by size:
4016 971 450 394 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4228 4262 1903
Size: 34 2291 34 3678
Fragments arranged by size:
3678 2291 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3151 3929 5139 3151
Size: 778 1210 4049
Fragments arranged by size:
4049 1210 778
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6037
BfrBI ATG'CAT
Cuts at: 3049 3049
Size: 6037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2603 4648 136
Size: 107 122 71 631 605 931 2045 1525
Fragments arranged by size:
2045 1525 931 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2271 6032 2271
Size: 3761 2276
BlpI GC'TnA_GC
Cuts at: 3454 3454
Size: 6037
Bme1580I G_kGCm'C
Cuts at: 1790 2805 3188 4098 5254 1790
Size: 1015 383 910 1156 2573
Fragments arranged by size:
2573 1156 1015 910 383
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2070 2486 3768
Size: 416 53 83 399 24 653 416 1282
Cuts at: 3768 4726 442
Size: 958 1753
Fragments arranged by size:
1753 1282 958 653 416 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2476 2474
Size: 2 6035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4697 1239
Size: 3458 2579
Bpu10I CC'TnA_GC
Cuts at: 3059 3059
Size: 6037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4162 5027 5325 4162
Size: 865 298 4874
Fragments arranged by size:
4874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2087 2447 4700 882
Size: 617 588 360 2253 2219
Fragments arranged by size:
2253 2219 617 588 360
BsaAI yAC'GTr
Cuts at: 493 1204 3828 493
Size: 711 2624 2702
Fragments arranged by size:
2702 2624 711
BsaBI GATnn'nnATC
Cuts at: 3071 6031 3071
Size: 2960 3077
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3844 4226 4861 275
Size: 53 83 186 936 2311 382 635 1451
Fragments arranged by size:
2311 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4470 4868 5142 5289 5700 4470
Size: 398 274 147 411 4807
Fragments arranged by size:
4807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5812
Fragments arranged by size:
5812 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 1186
Size: 1003 5034
BseYI C'CCAG_C
Cuts at: 2098 2344 5240 5736 2098
Size: 246 2896 496 2399
Fragments arranged by size:
2896 2399 496 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2838 1163
Size: 573 414 688 4362
Fragments arranged by size:
4362 688 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3487 4248 5276 1540
Size: 275 186 1486 761 1028 2301
Fragments arranged by size:
2301 1486 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4098 4183 5254 4098
Size: 85 1071 4881
Fragments arranged by size:
4881 1071 85
BsmI GAATG_Cn'
Cuts at: 3300 3548 3641 3300
Size: 248 93 5696
Fragments arranged by size:
5696 248 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2388 2424 2805 3188 3356 4098 4183
Size: 399 598 36 381 383 168 742 85
Cuts at: 4183 5254 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 598 399 383 381 168
85 36
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 2616 3270 3926 5656 2616
Size: 654 656 1730 2997
Fragments arranged by size:
2997 1730 656 654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3392 844
Size: 2548 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3924 1394
Size: 429 2101 3507
Fragments arranged by size:
3507 2101 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2599 4532 4706 65
Size: 1183 1351 1933 174 1396
Fragments arranged by size:
1933 1396 1351 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3255 4681 4868 1700
Size: 110 1445 1426 187 2869
Fragments arranged by size:
2869 1445 1426 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 4097 5109 4097
Size: 1012 5025
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3055 794
Size: 2261 3776
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 2924 1184
Size: 1740 4297
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2899 2902 2962 3001 2011
Size: 888 3 60 39 5047
Fragments arranged by size:
5047 888 60 39 3
BstYI r'GATC_y
Cuts at: 1972 2271 3367 3734 4119 4136 5588 5674 5686
Size: 299 1096 367 385 17 1452 86 12
Cuts at: 5686 6032 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1096 385 367 346 299 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5298
Fragments arranged by size:
5298 683 56
BtsI GCAGTG_nn'
Cuts at: 3201 3553 4347 4367 3201
Size: 352 794 20 4871
Fragments arranged by size:
4871 794 352 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 3341 3341
Size: 6037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5044 809
Size: 507 3728 1802
Fragments arranged by size:
3728 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2325 3257
Size: 54 1425 50 275 18 381 114 932
Cuts at: 3257 3484 4373 6002 8
Size: 227 889 1629 43
Fragments arranged by size:
1629 1425 932 889 381 275 227 114
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3484 1537
Size: 275 1672 4090
Fragments arranged by size:
4090 1672 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2187 3967 1353
Size: 789 45 1780 3423
Fragments arranged by size:
3423 1780 789 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4624 4998 5144 387
Size: 1064 138 3035 374 146 1280
Fragments arranged by size:
3035 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 4100 4697 5484 5752 1239
Size: 393 791 1677 597 787 268 1524
Fragments arranged by size:
1677 1524 791 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2438 2486 2045
Size: 393 48 5596
Fragments arranged by size:
5596 393 48
EcoO109I rG'GnC_Cy
Cuts at: 2398 2398
Size: 6037
EcoRI G'AATT_C
Cuts at: 3346 3724 3346
Size: 378 5659
FspI TGC'GCA
Cuts at: 4543 4543
Size: 6037
HaeII r_GCGC'y
Cuts at: 1678 5184 1678
Size: 3506 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4678 4710 4752 4784 1373
Size: 32 135 32 3106 32 42 32 2626
Fragments arranged by size:
3106 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2733 2764 3381 3627 669
Size: 988 281 795 31 617 246 3079
Fragments arranged by size:
3079 988 795 617 281 246 31
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KpnI G_GTAC'C
Cuts at: 2063 3362 2063
Size: 1299 4738
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3350 5151 5335 1305
Size: 2045 1801 184 2007
Fragments arranged by size:
2045 2007 1801 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 2327 10
Size: 54 1425 343 381 114 3720
Fragments arranged by size:
3720 1425 381 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2050 2798 2900 3248 3995 4354
Size: 648 843 41 748 102 348 747 359
Cuts at: 4354 4513 518
Size: 159 2042
Fragments arranged by size:
2042 843 748 747 648 359 348 159
102 41
MspA1I CmG'CkG
Cuts at: 1606 3453 4130 5278 5523 1606
Size: 1847 677 1148 245 2120
Fragments arranged by size:
2120 1847 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5485
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NsiI A_TGCA'T
Cuts at: 3051 3051
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 2320 3395 4940 1132
Size: 97 1091 1075 1545 2229
Fragments arranged by size:
2229 1545 1091 1075 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 4936 4936
Size: 6037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6037
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2466 2498 4106 4138 5619 5651 2466
Size: 32 1608 32 1481 32 2852
Fragments arranged by size:
2852 1608 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 3607 3607
Size: 6037
PstI C_TGCA'G
Cuts at: 830 3389 830
Size: 2559 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
SalI G'TCGA_C
Cuts at: 1936 2762 3379 1936
Size: 826 617 4594
Fragments arranged by size:
4594 826 617
SbfI CC_TGCA'GG
Cuts at: 3389 3389
Size: 6037
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SfcI C'TryA_G
Cuts at: 826 1045 3385 4520 5201 5392 826
Size: 219 2340 1135 681 191 1471
Fragments arranged by size:
2340 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 2419 3364 1872
Size: 547 945 4545
Fragments arranged by size:
4545 945 547
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4141 5042 5304 820
Size: 197 926 2198 901 262 1553
Fragments arranged by size:
2198 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SrfI GCCC'GGGC
Cuts at: 2419 2419
Size: 6037
SspI AAT'ATT
Cuts at: 5 52 3961 5
Size: 47 3909 2081
Fragments arranged by size:
3909 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6037
StyI C'CwwG_G
Cuts at: 513 1065 2139 2232 2322 2881 3476 513
Size: 552 1074 93 90 559 595 3074
Fragments arranged by size:
3074 1074 595 559 552 93 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 4064 4081 4234 4419 4864 325
Size: 1255 1332 1152 17 153 185 445 1498
Fragments arranged by size:
1498 1332 1255 1152 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 4283 5995
Size: 274 80 33 51 1443 807 1499 1712
Cuts at: 5995 96
Size: 138
Fragments arranged by size:
1712 1499 1443 807 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5997 6029 5997
Size: 32 6005
Tth111I GACn'n_nGTC
Cuts at: 1316 2747 1316
Size: 1431 4606
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2899 2962 2899
Size: 63 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 2417 3362 1870
Size: 547 945 4545
Fragments arranged by size:
4545 945 547
XmnI GAAnn'nnTTC
Cuts at: 4166 4166
Size: 6037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3845 276
Size: 53 83 186 936 2311 2468
Fragments arranged by size:
2468 2311 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SwaI