Restriction map for
pFN21AE6412
(Circular) (Six-base) MAPSORT of: pFN21AE6412.seq Check: 9695 from: 1 to: 6511
pFN21AE6412 6511 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4321 278
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2385 3854 1937
Size: 448 1469 4594
Fragments arranged by size:
4594 1469 448
Acc65I G'GTAC_C
Cuts at: 2525 3832 2525
Size: 1307 5204
AclI AA'CG_TT
Cuts at: 1563 4638 5011 1563
Size: 3075 373 3063
Fragments arranged by size:
3075 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2410 3667 4574 5958 6226 1632
Size: 778 1257 907 1384 268 1917
Fragments arranged by size:
1917 1384 1257 907 778 268
AfeI AGC'GCT
Cuts at: 3207 3428 3207
Size: 221 6290
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6314
AflIII A'CryG_T
Cuts at: 1203 2816 3216 3228 3327 3576 5410 6387 1203
Size: 1613 400 12 99 249 1834 977 1327
Fragments arranged by size:
1834 1613 1327 977 400 249 99 12
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 5240 5240
Size: 6511
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6511
AlwNI CAG_nnn'CTG
Cuts at: 2141 2605 2943 3068 4254 5826 2141
Size: 464 338 125 1186 1572 2826
Fragments arranged by size:
2826 1572 1186 464 338 125
ApaI G_GGCC'C
Cuts at: 2413 2614 3680 2413
Size: 201 1066 5244
Fragments arranged by size:
5244 1066 201
ApaLI G'TGCA_C
Cuts at: 3514 4568 5724 3514
Size: 1054 1156 4301
Fragments arranged by size:
4301 1156 1054
ApoI r'AATT_y
Cuts at: 1493 2787 3820 4046 4198 6307 1493
Size: 1294 1033 226 152 2109 1697
Fragments arranged by size:
2109 1697 1294 1033 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5065 160
Size: 872 4033 1606
Fragments arranged by size:
4033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 3836 1870
Size: 73 1893 4545
Fragments arranged by size:
4545 1893 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2039 2072 2039
Size: 33 6478
BamHI G'GATC_C
Cuts at: 2595 2827 3841 4208 2595
Size: 232 1014 367 4898
Fragments arranged by size:
4898 1014 367 232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2207 2525 2852 2889
Size: 325 217 627 300 120 318 327 37
Cuts at: 2889 2972 3037 3832 5287 618
Size: 83 65 795 1455 1842
Fragments arranged by size:
1842 1455 795 627 327 325 318 300
217 120 83 65 37
BanII G_rGCy'C
Cuts at: 1391 2413 2614 3680 3830 1391
Size: 1022 201 1066 150 4072
Fragments arranged by size:
4072 1066 1022 201 150
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 2251 2510 3453 928
Size: 971 325 27 259 943 3986
Fragments arranged by size:
3986 971 943 325 259 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3732 3766 4702 4736 1903
Size: 34 1795 34 936 34 3678
Fragments arranged by size:
3678 1795 936 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2067 4403 5613 2067
Size: 2336 1210 2965
Fragments arranged by size:
2965 2336 1210
BclI T'GATC_A
Cuts at: 1548 3300 1548
Size: 1752 4759
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2301 5122 136
Size: 107 122 71 631 605 629 2821 1525
Fragments arranged by size:
2821 1525 631 629 605 122 107 71
BglII A'GATC_T
Cuts at: 2322 2583 6506 2322
Size: 261 3923 2327
Fragments arranged by size:
3923 2327 261
BlpI GC'TnA_GC
Cuts at: 3928 3928
Size: 6511
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2413 2614 3042 3518 3680 3713 4572
Size: 316 307 201 428 476 162 33 859
Cuts at: 4572 5728 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 859 476 428 316 307 201
162 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6511
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4242 5200 442
Size: 416 53 83 399 24 2825 958 1753
Fragments arranged by size:
2825 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2191 2288 2335 3667 5171 1239
Size: 875 77 97 47 1332 1504 2579
Fragments arranged by size:
2579 1504 1332 875 97 77 47
Bpu10I CC'TnA_GC
Cuts at: 2158 2158
Size: 6511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3656 4636 5501 5799 3656
Size: 980 865 298 4368
Fragments arranged by size:
4368 980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2023 5174 882
Size: 617 524 3151 2219
Fragments arranged by size:
3151 2219 617 524
BsaAI yAC'GTr
Cuts at: 493 1204 3229 3328 3579 4302 493
Size: 711 2025 99 251 723 2702
Fragments arranged by size:
2702 2025 723 711 251 99
BsaBI GATnn'nnATC
Cuts at: 2369 6505 2369
Size: 4136 2375
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 4318 4700 5335
Size: 53 83 186 936 771 2014 382 635
Cuts at: 5335 275
Size: 1451
Fragments arranged by size:
2014 1451 936 771 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3246 4944 5342 5616 5763 6174 3246
Size: 1698 398 274 147 411 3583
Fragments arranged by size:
3583 1698 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3261 3291 1178
Size: 30 165 30 1858 30 4398
Fragments arranged by size:
4398 1858 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 3111 1186
Size: 1180 745 4586
Fragments arranged by size:
4586 1180 745
BseYI C'CCAG_C
Cuts at: 2141 2619 3712 3729 3739 5714 6210 2141
Size: 478 1093 17 10 1975 496 2442
Fragments arranged by size:
2442 1975 1093 496 478 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5938
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3961 4722 5750 1540
Size: 275 186 1960 761 1028 2301
Fragments arranged by size:
2301 1960 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3518 4572 4657 5728 3518
Size: 1054 85 1071 4301
Fragments arranged by size:
4301 1071 1054 85
BsmI GAATG_Cn'
Cuts at: 2250 2542 3636 4022 4115 2250
Size: 292 1094 386 93 4646
Fragments arranged by size:
4646 1094 386 292 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3286 3286
Size: 6511
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2413 2614 3042 3518 3680 3713
Size: 399 316 307 201 428 476 162 33
Cuts at: 3713 3830 4572 4657 5728 1391
Size: 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 428 399 316 307
201 162 117 85 33
BspEI T'CCGG_A
Cuts at: 6174 6174
Size: 6511
BspHI T'CATG_A
Cuts at: 4400 6130 4400
Size: 1730 4781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 3866 844
Size: 1610 1412 3489
Fragments arranged by size:
3489 1610 1412
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 2569 4398 1394
Size: 429 275 471 1829 3507
Fragments arranged by size:
3507 1829 471 429 275
BsrDI GCAATG_nn'
Cuts at: 65 1248 5006 5180 65
Size: 1183 3758 174 1396
Fragments arranged by size:
3758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2987 5155 5342 1700
Size: 110 1177 2168 187 2869
Fragments arranged by size:
2869 2168 1177 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6511
BssSI C'ACGA_G
Cuts at: 4571 5583 4571
Size: 1012 5499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3037 794
Size: 2243 4268
BstBI TT'CG_AA
Cuts at: 5315 5315
Size: 6511
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6511
BstXI CCAn_nnnn'nTGG
Cuts at: 2983 2983
Size: 6511
BstYI r'GATC_y
Cuts at: 1972 2322 2583 2595 2827 3841 4208 4593 4610
Size: 350 261 12 232 1014 367 385 17
Cuts at: 4610 6062 6148 6160 6506 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1014 385 367 350 346 261
232 86 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3092 3467 3584 3627 513
Size: 552 939 1088 375 117 43 3397
Fragments arranged by size:
3397 1088 939 552 375 117 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2932 3067 3609 487
Size: 683 56 1706 135 542 3389
Fragments arranged by size:
3389 1706 683 542 135 56
BtsI GCAGTG_nn'
Cuts at: 3776 3805 4027 4821 4841 3776
Size: 29 222 794 20 5446
Fragments arranged by size:
5446 794 222 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6476
DraI TTT'AAA
Cuts at: 3815 3815
Size: 6511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5518 809
Size: 507 4202 1802
Fragments arranged by size:
4202 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2715 2985 3054
Size: 54 1425 50 275 18 885 270 69
Cuts at: 3054 3354 3381 3958 4847 6476 8
Size: 300 27 577 889 1629 43
Fragments arranged by size:
1629 1425 889 885 577 300 275 270
69 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3958 1537
Size: 275 2146 4090
Fragments arranged by size:
4090 2146 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2429 3500 3536 4441 1353
Size: 1076 1071 36 905 3423
Fragments arranged by size:
3423 1076 1071 905 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3333 5098 5472 5618 387
Size: 1064 138 1744 1765 374 146 1280
Fragments arranged by size:
1765 1744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2191 2288 2335 2410 3667 4574
Size: 393 482 77 97 47 75 1257 907
Cuts at: 4574 5171 5958 6226 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1257 907 787 597 482 393 268
97 77 75 47
EcoO109I rG'GnC_Cy
Cuts at: 2410 2610 2611 3065 3676 3677 3746 3771 2410
Size: 200 1 454 611 1 69 25 5150
Fragments arranged by size:
5150 611 454 200 69 25 1 1
EcoRI G'AATT_C
Cuts at: 2787 3820 4198 2787
Size: 1033 378 5100
Fragments arranged by size:
5100 1033 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3697 3697
Size: 6511
FspI TGC'GCA
Cuts at: 5017 5017
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 3209 3430 5658 1678
Size: 1531 221 2228 2531
Fragments arranged by size:
2531 2228 1531 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3261 3293 3678 3682 3710
Size: 32 135 32 1689 32 385 4 28
Cuts at: 3710 3714 3785 3817 5152 5184 5226 5258 1373
Size: 4 71 32 1335 32 42 32 2626
Fragments arranged by size:
2626 1689 1335 385 135 71 42 32
32 32 32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3855 4101 669
Size: 988 281 1917 246 3079
Fragments arranged by size:
3079 1917 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6511
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KpnI G_GTAC'C
Cuts at: 2529 3836 2529
Size: 1307 5204
MfeI C'AATT_G
Cuts at: 4110 4110
Size: 6511
MluI A'CGCG_T
Cuts at: 2816 6387 2816
Size: 3571 2940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5625 5809 1305
Size: 4320 184 2007
Fragments arranged by size:
4320 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3383 10
Size: 54 1425 343 1551 3138
Fragments arranged by size:
3138 1551 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 2738 4469 4828 4987 518
Size: 648 1230 342 1731 359 159 2042
Fragments arranged by size:
2042 1731 1230 648 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2970 3094 3509 3556 3733 3927 4604 5752
Size: 1364 124 415 47 177 194 677 1148
Cuts at: 5752 5997 1606
Size: 245 2120
Fragments arranged by size:
2120 1364 1148 677 415 245 194 177
124 47
NaeI GCC'GGC
Cuts at: 2989 2989
Size: 6511
NcoI C'CATG_G
Cuts at: 513 1065 2004 3467 3627 513
Size: 552 939 1463 160 3397
Fragments arranged by size:
3397 1463 939 552 160
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6511
NgoMIV G'CCGG_C
Cuts at: 2987 2987
Size: 6511
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6511
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NspI r_CATG'y
Cuts at: 1132 1229 3220 3367 3869 5414 1132
Size: 97 1991 147 502 1545 2229
Fragments arranged by size:
2229 1991 1545 502 147 97
PasI CC'CwG_GG
Cuts at: 1348 2451 2902 3601 1348
Size: 1103 451 699 4258
Fragments arranged by size:
4258 1103 699 451
PciI A'CATG_T
Cuts at: 3216 5410 3216
Size: 2194 4317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 1108
Size: 1497 5014
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PmlI CAC'GTG
Cuts at: 3229 3229
Size: 6511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 6093 6125 4580
Size: 32 1481 32 4966
Fragments arranged by size:
4966 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3746 3771 3746
Size: 25 6486
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6511
PsiI TTA'TAA
Cuts at: 4081 4081
Size: 6511
PspOMI G'GGCC_C
Cuts at: 2409 2610 3676 2409
Size: 201 1066 5244
Fragments arranged by size:
5244 1066 201
PstI C_TGCA'G
Cuts at: 830 3863 830
Size: 3033 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
PvuII CAG'CTG
Cuts at: 3509 3509
Size: 6511
SacII CC_GC'GG
Cuts at: 3095 3095
Size: 6511
SalI G'TCGA_C
Cuts at: 1936 3853 1936
Size: 1917 4594
SapI GCTCTTCn'nnn_
Cuts at: 3500 3500
Size: 6511
SbfI CC_TGCA'GG
Cuts at: 3863 3863
Size: 6511
ScaI AGT'ACT
Cuts at: 4759 6471 4759
Size: 1712 4799
SfcI C'TryA_G
Cuts at: 826 1045 2935 3655 3859 4994 5675 5866 826
Size: 219 1890 720 204 1135 681 191 1471
Fragments arranged by size:
1890 1471 1135 720 681 219 204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6511
SmaI CCC'GGG
Cuts at: 1872 3838 1872
Size: 1966 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3671 4615 5516 5778 820
Size: 197 926 1728 944 901 262 1553
Fragments arranged by size:
1728 1553 944 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6511
SphI G_CATG'C
Cuts at: 1132 3869 1132
Size: 2737 3774
SspI AAT'ATT
Cuts at: 5 52 4435 5
Size: 47 4383 2081
Fragments arranged by size:
4383 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6511
StyI C'CwwG_G
Cuts at: 513 1065 2004 2782 3265 3467 3627 3950 513
Size: 552 939 778 483 202 160 323 3074
Fragments arranged by size:
3074 939 778 552 483 323 202 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2870 2877 4538 4555 4708 4893 5338
Size: 1255 1290 7 1661 17 153 185 445
Cuts at: 5338 325
Size: 1498
Fragments arranged by size:
1661 1498 1290 1255 445 185 153 17
7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2286 2706 3546
Size: 274 80 33 51 1443 309 420 840
Cuts at: 3546 3573 4757 6469 96
Size: 27 1184 1712 138
Fragments arranged by size:
1712 1443 1184 840 420 309 274 138
80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3312 3344 6471 6503 3312
Size: 32 3127 32 3320
Fragments arranged by size:
3320 3127 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6511
XbaI T'CTAG_A
Cuts at: 3847 3847
Size: 6511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2698 3049 2698
Size: 351 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6511
XmaI C'CCGG_G
Cuts at: 1870 3836 1870
Size: 1966 4545
XmnI GAAnn'nnTTC
Cuts at: 4640 4640
Size: 6511
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4319 276
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI