Restriction map for
pFN21AE6411
(Circular) (Six-base) MAPSORT of: pFN21AE6411.seq Check: 6259 from: 1 to: 6511
pFN21AE6411 6511 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4321 278
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3854 1937
Size: 1917 4594
Acc65I G'GTAC_C
Cuts at: 3832 3832
Size: 6511
AclI AA'CG_TT
Cuts at: 1563 4638 5011 1563
Size: 3075 373 3063
Fragments arranged by size:
3075 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2905 4574 5958 6226 1632
Size: 1273 1669 1384 268 1917
Fragments arranged by size:
1917 1669 1384 1273 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6314
AflIII A'CryG_T
Cuts at: 1203 5410 6387 1203
Size: 4207 977 1327
Fragments arranged by size:
4207 1327 977
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 5240 5240
Size: 6511
AleI CACnn'nnGTG
Cuts at: 1166 3149 1166
Size: 1983 4528
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6511
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 6479
AlwNI CAG_nnn'CTG
Cuts at: 2549 2928 3007 4254 5826 2549
Size: 379 79 1247 1572 3234
Fragments arranged by size:
3234 1572 1247 379 79
ApaI G_GGCC'C
Cuts at: 3015 3015
Size: 6511
ApaLI G'TGCA_C
Cuts at: 2161 3515 4568 5724 2161
Size: 1354 1053 1156 2948
Fragments arranged by size:
2948 1354 1156 1053
ApoI r'AATT_y
Cuts at: 1493 2824 3820 4046 4198 6307 1493
Size: 1331 996 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 996 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5065 160
Size: 872 4033 1606
Fragments arranged by size:
4033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 2976 3078 3836 1870
Size: 73 1033 102 758 4545
Fragments arranged by size:
4545 1033 758 102 73
BamHI G'GATC_C
Cuts at: 3841 4208 3841
Size: 367 6144
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3725 3832 5287 618
Size: 325 217 627 1938 107 1455 1842
Fragments arranged by size:
1938 1842 1455 627 325 217 107
BanII G_rGCy'C
Cuts at: 1391 2977 3015 3246 3830 1391
Size: 1586 38 231 584 4072
Fragments arranged by size:
4072 1586 584 231 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 928
Size: 971 190 5350
Fragments arranged by size:
5350 971 190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4702 4736 1903
Size: 34 2765 34 3678
Fragments arranged by size:
3678 2765 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4403 5613 4403
Size: 1210 5301
BclI T'GATC_A
Cuts at: 1548 2295 1548
Size: 747 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2380 3032
Size: 107 122 71 631 605 337 371 652
Cuts at: 3032 5122 136
Size: 2090 1525
Fragments arranged by size:
2090 1525 652 631 605 371 337 122
107 71
BglII A'GATC_T
Cuts at: 6506 6506
Size: 6511
BlpI GC'TnA_GC
Cuts at: 2429 3928 2429
Size: 1499 5012
Bme1580I G_kGCm'C
Cuts at: 1790 2165 2580 2744 2762 3015 3282 3393 3519
Size: 375 415 164 18 253 267 111 126
Cuts at: 3519 3545 3706 3728 4572 5728 1790
Size: 26 161 22 844 1156 2573
Fragments arranged by size:
2573 1156 844 415 375 267 253 164
161 126 111 26 22 18
BmgBI CAC'GTC
Cuts at: 1318 2818 1318
Size: 1500 5011
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2248 3748 4242
Size: 416 53 83 399 24 831 1500 494
Cuts at: 4242 5200 442
Size: 958 1753
Fragments arranged by size:
1753 1500 958 831 494 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2647 2950 3401 5171 1239
Size: 1408 303 451 1770 2579
Fragments arranged by size:
2579 1770 1408 451 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2777 3185 4636 5501 5799 2330
Size: 447 408 1451 865 298 3042
Fragments arranged by size:
3042 1451 865 447 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3583 5174 882
Size: 617 2084 1591 2219
Fragments arranged by size:
2219 2084 1591 617
BsaAI yAC'GTr
Cuts at: 493 1204 4302 493
Size: 711 3098 2702
Fragments arranged by size:
3098 2702 711
BsaBI GATnn'nnATC
Cuts at: 2679 6505 2679
Size: 3826 2685
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4318 4700 5335 275
Size: 53 83 186 936 2785 382 635 1451
Fragments arranged by size:
2785 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4944 5342 5616 5763 6174 4944
Size: 398 274 147 411 5281
Fragments arranged by size:
5281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 2576 2606 1178
Size: 30 165 30 1023 30 120 30 5083
Fragments arranged by size:
5083 1023 165 120 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 3059 1186
Size: 1144 729 4638
Fragments arranged by size:
4638 1144 729
BseYI C'CCAG_C
Cuts at: 2519 3102 3721 5714 6210 2519
Size: 583 619 1993 496 2820
Fragments arranged by size:
2820 1993 619 583 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 3356 1163
Size: 573 576 1044 4318
Fragments arranged by size:
4318 1044 576 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3961 4722 5750 1540
Size: 275 186 1960 761 1028 2301
Fragments arranged by size:
2301 1960 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2165 3519 4572 4657 5728 2165
Size: 1354 1053 85 1071 2948
Fragments arranged by size:
2948 1354 1071 1053 85
BsmI GAATG_Cn'
Cuts at: 4022 4115 4022
Size: 93 6418
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2580 2744 2762 2977 3015 3246
Size: 399 375 415 164 18 215 38 231
Cuts at: 3246 3282 3393 3519 3545 3706 3728 3830 4572
Size: 36 111 126 26 161 22 102 742
Cuts at: 4572 4657 5728 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 375 231 215
164 161 126 111 102 85 38 36
26 22 18
BspEI T'CCGG_A
Cuts at: 6174 6174
Size: 6511
BspHI T'CATG_A
Cuts at: 4400 6130 4400
Size: 1730 4781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 3866 844
Size: 1490 1532 3489
Fragments arranged by size:
3489 1532 1490
BsrBI CCG'CTC
Cuts at: 1394 1823 2077 2179 4398 1394
Size: 429 254 102 2219 3507
Fragments arranged by size:
3507 2219 429 254 102
BsrDI GCAATG_nn'
Cuts at: 65 1248 2463 3770 5006 5180 65
Size: 1183 1215 1307 1236 174 1396
Fragments arranged by size:
1396 1307 1236 1215 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2089 2331 2374 2653 3146 3481 5155
Size: 110 279 242 43 279 493 335 1674
Cuts at: 5155 5342 1700
Size: 187 2869
Fragments arranged by size:
2869 1674 493 335 279 279 242 187
110 43
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6511
BssHII G'CGCG_C
Cuts at: 1925 2043 1925
Size: 118 6393
BssSI C'ACGA_G
Cuts at: 4571 5583 4571
Size: 1012 5499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6511
BstBI TT'CG_AA
Cuts at: 2509 5315 2509
Size: 2806 3705
BstEII G'GTnAC_C
Cuts at: 1184 2401 1184
Size: 1217 5294
BstYI r'GATC_y
Cuts at: 1972 2349 2647 2892 3841 4208 4593 4610 6062
Size: 377 298 245 949 367 385 17 1452
Cuts at: 6062 6148 6160 6506 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 949 385 377 367 346 298
245 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 3318 513
Size: 552 939 576 738 3706
Fragments arranged by size:
3706 939 738 576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3412 3583 487
Size: 683 56 2186 171 3415
Fragments arranged by size:
3415 2186 683 171 56
BtsI GCAGTG_nn'
Cuts at: 2445 2816 4027 4821 4841 2445
Size: 371 1211 794 20 4115
Fragments arranged by size:
4115 1211 794 371 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3765 3800 529
Size: 35 3201 35 3240
Fragments arranged by size:
3240 3201 35 35
DraI TTT'AAA
Cuts at: 3815 3815
Size: 6511
DraIII CAC_nnn'GTG
Cuts at: 2575 2575
Size: 6511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5518 809
Size: 507 4202 1802
Fragments arranged by size:
4202 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2301 2376
Size: 54 1425 50 275 18 195 276 75
Cuts at: 2376 2494 2785 3321 3498 3786 3958 4847 6476
Size: 118 291 536 177 288 172 889 1629
Cuts at: 6476 8
Size: 43
Fragments arranged by size:
1629 1425 889 536 291 288 276 275
195 177 172 118 75 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3958 1537
Size: 275 2146 4090
Fragments arranged by size:
4090 2146 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 2529 3077 3119 4441 1353
Size: 932 244 548 42 1322 3423
Fragments arranged by size:
3423 1322 932 548 244 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3494 5098 5472 5618 387
Size: 1064 138 1905 1604 374 146 1280
Fragments arranged by size:
1905 1604 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2647 2905 2950 3401 4574 5171 5958
Size: 393 1015 258 45 451 1173 597 787
Cuts at: 5958 6226 1239
Size: 268 1524
Fragments arranged by size:
1524 1173 1015 787 597 451 393 268
258 45
EcoO109I rG'GnC_Cy
Cuts at: 2170 2515 3011 3082 3192 2170
Size: 345 496 71 110 5489
Fragments arranged by size:
5489 496 345 110 71
EcoRI G'AATT_C
Cuts at: 3820 4198 3820
Size: 378 6133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 6511
FspI TGC'GCA
Cuts at: 5017 5017
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 2015 2195 2310 5658 1678
Size: 337 180 115 3348 2531
Fragments arranged by size:
3348 2531 337 180 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3737 3769 5152 5184 5226
Size: 32 135 32 2165 32 1383 32 42
Cuts at: 5226 5258 1373
Size: 32 2626
Fragments arranged by size:
2626 2165 1383 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3855 4101 669
Size: 988 281 1917 246 3079
Fragments arranged by size:
3079 1917 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6511
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KpnI G_GTAC'C
Cuts at: 3836 3836
Size: 6511
MfeI C'AATT_G
Cuts at: 4110 4110
Size: 6511
MluI A'CGCG_T
Cuts at: 6387 6387
Size: 6511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 2344 2666 5625 5809 1305
Size: 957 82 322 2959 184 2007
Fragments arranged by size:
2959 2007 957 322 184 82
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2496 2787 3323 3500
Size: 54 1425 343 471 193 291 536 177
Cuts at: 3500 3788 10
Size: 288 2733
Fragments arranged by size:
2733 1425 536 471 343 291 288 193
177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2450 2585 3149 3419 4469 4828
Size: 648 984 300 135 564 270 1050 359
Cuts at: 4828 4987 518
Size: 159 2042
Fragments arranged by size:
2042 1050 984 648 564 359 300 270
159 135
MspA1I CmG'CkG
Cuts at: 1606 2024 2124 2546 3370 3721 3927 4604 5752
Size: 418 100 422 824 351 206 677 1148
Cuts at: 5752 5997 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 824 677 422 418 351 245
206 100
NaeI GCC'GGC
Cuts at: 2376 2655 2376
Size: 279 6232
NcoI C'CATG_G
Cuts at: 513 1065 2004 3318 513
Size: 552 939 1314 3706
Fragments arranged by size:
3706 1314 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6511
NgoMIV G'CCGG_C
Cuts at: 2374 2653 2374
Size: 279 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6511
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NspI r_CATG'y
Cuts at: 1132 1229 3058 3869 5414 1132
Size: 97 1829 811 1545 2229
Fragments arranged by size:
2229 1829 1545 811 97
PasI CC'CwG_GG
Cuts at: 1348 2624 1348
Size: 1276 5235
PciI A'CATG_T
Cuts at: 5410 5410
Size: 6511
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3007 1108
Size: 1899 4612
PfoI T'CCnGG_A
Cuts at: 2663 3732 2663
Size: 1069 5442
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 6093 6125 4580
Size: 32 1481 32 4966
Fragments arranged by size:
4966 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2515 3082 2515
Size: 567 5944
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6511
PsiI TTA'TAA
Cuts at: 4081 4081
Size: 6511
PspOMI G'GGCC_C
Cuts at: 3011 3011
Size: 6511
PspXI vC'TCGA_Gb
Cuts at: 2976 2976
Size: 6511
PstI C_TGCA'G
Cuts at: 830 3863 830
Size: 3033 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
PvuII CAG'CTG
Cuts at: 2546 3370 3721 2546
Size: 824 351 5336
Fragments arranged by size:
5336 824 351
SalI G'TCGA_C
Cuts at: 1936 3853 1936
Size: 1917 4594
SanDI GG'GwC_CC
Cuts at: 3082 3082
Size: 6511
SbfI CC_TGCA'GG
Cuts at: 3863 3863
Size: 6511
ScaI AGT'ACT
Cuts at: 2258 4759 6471 2258
Size: 2501 1712 2298
Fragments arranged by size:
2501 2298 1712
SfcI C'TryA_G
Cuts at: 826 1045 3859 4994 5675 5866 826
Size: 219 2814 1135 681 191 1471
Fragments arranged by size:
2814 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 2331 3146 1700
Size: 631 815 5065
Fragments arranged by size:
5065 815 631
SmaI CCC'GGG
Cuts at: 1872 3080 3838 1872
Size: 1208 758 4545
Fragments arranged by size:
4545 1208 758
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 2792 2976 3200 4615 5516
Size: 197 926 402 447 184 224 1415 901
Cuts at: 5516 5778 820
Size: 262 1553
Fragments arranged by size:
1553 1415 926 901 447 402 262 224
197 184
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6511
SphI G_CATG'C
Cuts at: 1132 3869 1132
Size: 2737 3774
SspI AAT'ATT
Cuts at: 5 52 4435 5
Size: 47 4383 2081
Fragments arranged by size:
4383 2081 47
StuI AGG'CCT
Cuts at: 1517 3648 1517
Size: 2131 4380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 3318 3783 3950 513
Size: 552 939 108 1206 465 167 3074
Fragments arranged by size:
3074 1206 939 552 465 167 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3656 3745 3793 4538 4555 4708 4893
Size: 1255 2076 89 48 745 17 153 185
Cuts at: 4893 5338 325
Size: 445 1498
Fragments arranged by size:
2076 1498 1255 745 445 185 153 89
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 2256 3750
Size: 274 80 33 51 1443 177 102 1494
Cuts at: 3750 4757 6469 96
Size: 1007 1712 138
Fragments arranged by size:
1712 1494 1443 1007 274 177 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6471 6503 6471
Size: 32 6479
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6511
XbaI T'CTAG_A
Cuts at: 3847 3847
Size: 6511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2386 3718 2011
Size: 375 1332 4804
Fragments arranged by size:
4804 1332 375
XhoI C'TCGA_G
Cuts at: 1943 2976 1943
Size: 1033 5478
XmaI C'CCGG_G
Cuts at: 1870 3078 3836 1870
Size: 1208 758 4545
Fragments arranged by size:
4545 1208 758
XmnI GAAnn'nnTTC
Cuts at: 2702 4640 2702
Size: 1938 4573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4319 276
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI