Restriction map for
pFN21AE6404
(Circular) (Six-base) MAPSORT of: pFN21AE6404.seq Check: 9956 from: 1 to: 6067
pFN21AE6404 6067 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2793 2793
Size: 6067
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 3410 1937
Size: 1243 230 4594
Fragments arranged by size:
4594 1243 230
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 4130 5514 5782 1632
Size: 758 1740 1384 268 1917
Fragments arranged by size:
1917 1740 1384 758 268
AfeI AGC'GCT
Cuts at: 3112 3112
Size: 6067
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 3201 4966 5943 1203
Size: 1998 1765 977 1327
Fragments arranged by size:
1998 1765 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 2147 3224 1166
Size: 981 1077 4009
Fragments arranged by size:
4009 1077 981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2368 2581 2785 3810 5382 2368
Size: 213 204 1025 1572 3053
Fragments arranged by size:
3053 1572 1025 213 204
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 3376 3602 3754 5863 1493
Size: 1883 226 152 2109 1697
Fragments arranged by size:
2109 1883 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2175 2917 3068 3276 3392 1870
Size: 73 232 742 151 208 116 4545
Fragments arranged by size:
4545 742 232 208 151 116 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2507 2921 3388 4843 618
Size: 325 217 627 720 414 467 1455 1842
Fragments arranged by size:
1842 1455 720 627 467 414 325 217
BanII G_rGCy'C
Cuts at: 1391 2405 3000 3386 1391
Size: 1014 595 386 4072
Fragments arranged by size:
4072 1014 595 386
BbeI G_GCGC'C
Cuts at: 2925 2925
Size: 6067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5096
BbvCI CC'TCA_GC
Cuts at: 2110 2110
Size: 6067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BfrBI ATG'CAT
Cuts at: 2136 2136
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2552 2919 4678
Size: 107 122 71 631 605 880 367 1759
Cuts at: 4678 136
Size: 1525
Fragments arranged by size:
1759 1525 880 631 605 367 122 107
71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2810 3231 4128 5284 1790
Size: 1020 421 897 1156 2573
Fragments arranged by size:
2573 1156 1020 897 421
BmgBI CAC'GTC
Cuts at: 1318 3204 3226 3343 1318
Size: 1886 22 117 4042
Fragments arranged by size:
4042 1886 117 22
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2227 2670 3798
Size: 416 53 83 399 24 810 443 1128
Cuts at: 3798 4756 442
Size: 958 1753
Fragments arranged by size:
1753 1128 958 810 443 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2432 2570 2753 4727 1239
Size: 1019 174 138 183 1974 2579
Fragments arranged by size:
2579 1974 1019 183 174 138
Bpu10I CC'TnA_GC
Cuts at: 2050 2110 2399 2050
Size: 60 289 5718
Fragments arranged by size:
5718 289 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2270 4192 5057 5355 2270
Size: 1922 865 298 2982
Fragments arranged by size:
2982 1922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2288 4730 882
Size: 617 789 2442 2219
Fragments arranged by size:
2442 2219 789 617
BsaAI yAC'GTr
Cuts at: 493 1204 2818 3148 3858 493
Size: 711 1614 330 710 2702
Fragments arranged by size:
2702 1614 711 710 330
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2922 3874 4256 4891
Size: 53 83 186 936 1389 952 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1451 1389 952 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2816 2846 1178
Size: 30 165 30 1413 30 4399
Fragments arranged by size:
4399 1413 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 2513 2923 1186
Size: 1285 42 410 4330
Fragments arranged by size:
4330 1285 410 42
BseYI C'CCAG_C
Cuts at: 2155 2581 2793 3167 5270 5766 2155
Size: 426 212 374 2103 496 2456
Fragments arranged by size:
2456 2103 496 426 374 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2125 2924 1163
Size: 573 389 799 4306
Fragments arranged by size:
4306 799 573 389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2148 3517 4278 5306 1540
Size: 275 186 147 1369 761 1028 2301
Fragments arranged by size:
2301 1369 1028 761 275 186 147
BsiHKAI G_wGCw'C
Cuts at: 2051 2405 3144 3321 4128 4213 5284 2051
Size: 354 739 177 807 85 1071 2834
Fragments arranged by size:
2834 1071 807 739 354 177 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
BsmBI CGTCTCn'nnnn_
Cuts at: 2233 2233
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2405 2810 3000 3144 3231 3321
Size: 399 261 354 405 190 144 87 90
Cuts at: 3321 3386 4128 4213 5284 1391
Size: 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 354 261 190
144 90 87 85 65
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3126 3956 5686 3126
Size: 830 1730 3507
Fragments arranged by size:
3507 1730 830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2177 2535 2793 3422 844
Size: 1333 358 258 629 3489
Fragments arranged by size:
3489 1333 629 358 258
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 2886 2994 3954 1394
Size: 429 191 872 108 960 3507
Fragments arranged by size:
3507 960 872 429 191 108
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2924 3048 3092 4711 4898 1700
Size: 110 1114 124 44 1619 187 2869
Fragments arranged by size:
2869 1619 1114 187 124 110 44
BsrGI T'GTAC_A
Cuts at: 96 2103 2823 3009 96
Size: 2007 720 186 3154
Fragments arranged by size:
3154 2007 720 186
BssHII G'CGCG_C
Cuts at: 1925 3283 1925
Size: 1358 4709
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2788 794
Size: 1994 4073
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstXI CCAn_nnnn'nTGG
Cuts at: 3167 3167
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062
Size: 1425 367 385 17 1452 86 12 346
Cuts at: 6062 1972
Size: 1977
Fragments arranged by size:
1977 1452 1425 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2238 2631 2798 513
Size: 552 1173 393 167 3782
Fragments arranged by size:
3782 1173 552 393 167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 4377 4397 3583
Size: 794 20 5253
Fragments arranged by size:
5253 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DraIII CAC_nnn'GTG
Cuts at: 2821 3316 2821
Size: 495 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2009 2058 2145
Size: 54 1425 50 275 18 179 49 87
Cuts at: 2145 2349 2487 3307 3514 4403 6032 8
Size: 204 138 820 207 889 1629 43
Fragments arranged by size:
1629 1425 889 820 275 207 204 179
138 87 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2145 3514 1537
Size: 275 333 1369 4090
Fragments arranged by size:
4090 1369 333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2082 3997 1353
Size: 725 4 1915 3423
Fragments arranged by size:
3423 1915 725 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2403 2403
Size: 6067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2390 2432 2570 2753 4130 4727
Size: 393 626 132 42 138 183 1377 597
Cuts at: 4727 5514 5782 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1377 787 626 597 393 268 183
138 132 42
EcoO109I rG'GnC_Cy
Cuts at: 2119 2245 2340 2524 2637 2728 3361 2119
Size: 126 95 184 113 91 633 4825
Fragments arranged by size:
4825 633 184 126 113 95 91
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 6067
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2925 3114 5214 1678
Size: 1247 189 2100 2531
Fragments arranged by size:
2531 2100 1247 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2055 2087 2742 2774 4708
Size: 32 135 32 483 32 655 32 1934
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1934 655 483 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KasI G'GCGC_C
Cuts at: 2921 2921
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2991 5181 5365 1305
Size: 1686 2190 184 2007
Fragments arranged by size:
2190 2007 1686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 3224 3257 4025 4384 4543 518
Size: 648 981 1077 33 768 359 159 2042
Fragments arranged by size:
2042 1077 981 768 648 359 159 33
MspA1I CmG'CkG
Cuts at: 1606 2032 2101 2333 3483 4160 5308 5553 1606
Size: 426 69 232 1150 677 1148 245 2120
Fragments arranged by size:
2120 1150 1148 677 426 245 232 69
NaeI GCC'GGC
Cuts at: 3050 3050
Size: 6067
NarI GG'CG_CC
Cuts at: 2922 2922
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 2238 2631 513
Size: 552 1173 393 3949
Fragments arranged by size:
3949 1173 552 393
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NgoMIV G'CCGG_C
Cuts at: 3048 3048
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NruI TCG'CGA
Cuts at: 2268 2268
Size: 6067
NsiI A_TGCA'T
Cuts at: 2138 2138
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2746 1348
Size: 1398 4669
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2581 1108
Size: 1131 342 4594
Fragments arranged by size:
4594 1131 342
PfoI T'CCnGG_A
Cuts at: 2361 2409 2651 2730 2361
Size: 48 242 79 5698
Fragments arranged by size:
5698 242 79 48
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PmlI CAC'GTG
Cuts at: 2818 2818
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2340 2524 2637 2728 3361 2245
Size: 95 184 113 91 633 4951
Fragments arranged by size:
4951 633 184 113 95 91
PshAI GACnn'nnGTC
Cuts at: 1613 2603 1613
Size: 990 5077
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspXI vC'TCGA_Gb
Cuts at: 3068 3068
Size: 6067
PstI C_TGCA'G
Cuts at: 830 2532 3419 830
Size: 1702 887 3478
Fragments arranged by size:
3478 1702 887
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2101 2333 2101
Size: 232 5835
SacI G_AGCT'C
Cuts at: 2405 2405
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SanDI GG'GwC_CC
Cuts at: 2340 2637 3361 2340
Size: 297 724 5046
Fragments arranged by size:
5046 724 297
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SexAI A'CCwGG_T
Cuts at: 3312 3312
Size: 6067
SfcI C'TryA_G
Cuts at: 826 1045 2528 3415 4550 5231 5422 826
Size: 219 1483 887 1135 681 191 1471
Fragments arranged by size:
1483 1471 1135 887 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2552 2552
Size: 6067
SfoI GGC'GCC
Cuts at: 2923 2923
Size: 6067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 2919 3278 3394 1872
Size: 1047 359 116 4545
Fragments arranged by size:
4545 1047 359 116
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2175 2249 3068 4171 5072 5334
Size: 197 926 232 74 819 1103 901 262
Cuts at: 5334 820
Size: 1553
Fragments arranged by size:
1553 1103 926 901 819 262 232 197
74
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 2055 3122 1517
Size: 538 1067 4462
Fragments arranged by size:
4462 1067 538
StyI C'CwwG_G
Cuts at: 513 1065 2238 2631 3174 3506 513
Size: 552 1173 393 543 332 3074
Fragments arranged by size:
3074 1173 552 543 393 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2395 2495 4094 4111 4264 4449 4894
Size: 1255 815 100 1599 17 153 185 445
Cuts at: 4894 325
Size: 1498
Fragments arranged by size:
1599 1498 1255 815 445 185 153 100
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 2418 2823
Size: 274 80 33 51 1443 126 315 405
Cuts at: 2823 3009 3216 4313 6025 96
Size: 186 207 1097 1712 138
Fragments arranged by size:
1712 1443 1097 405 315 274 207 186
138 126 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2091 3160 2091
Size: 1069 4998
XhoI C'TCGA_G
Cuts at: 1943 2175 3068 1943
Size: 232 893 4942
Fragments arranged by size:
4942 893 232
XmaI C'CCGG_G
Cuts at: 1870 2917 3276 3392 1870
Size: 1047 359 116 4545
Fragments arranged by size:
4545 1047 359 116
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BplI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FseI FspAI PacI PspOMI PsrI
RsrII SacII SapI SrfI SwaI