Restriction map for
pFN21AE6403
(Circular) (Six-base) MAPSORT of: pFN21AE6403.seq Check: 5794 from: 1 to: 6238
pFN21AE6403 6238 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2083 2083
Size: 6238
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3581 1937
Size: 1644 4594
Acc65I G'GTAC_C
Cuts at: 3447 3559 3447
Size: 112 6126
AclI AA'CG_TT
Cuts at: 1563 3537 4365 4738 1563
Size: 1974 828 373 3063
Fragments arranged by size:
3063 1974 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2772 3148 4301 5685 5953 1632
Size: 491 649 376 1153 1384 268 1917
Fragments arranged by size:
1917 1384 1153 649 491 376 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 5137 6114 1203
Size: 3934 977 1327
Fragments arranged by size:
3934 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 3077 1166
Size: 1911 4327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2819 1184
Size: 1635 4603
AlwNI CAG_nnn'CTG
Cuts at: 2364 3171 3460 3476 3981 5553 2364
Size: 807 289 16 505 1572 3049
Fragments arranged by size:
3049 1572 807 505 289 16
ApaI G_GGCC'C
Cuts at: 2266 2266
Size: 6238
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2650 3547 3773 3925 6034 1493
Size: 1157 897 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 897 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 2161 2449 3563 1870
Size: 73 218 288 1114 4545
Fragments arranged by size:
4545 1114 288 218 73
BamHI G'GATC_C
Cuts at: 3523 3568 3935 3523
Size: 45 367 5826
Fragments arranged by size:
5826 367 45
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2285 3447 3559 5014 618
Size: 325 217 627 498 1162 112 1455 1842
Fragments arranged by size:
1842 1455 1162 627 498 325 217 112
BanII G_rGCy'C
Cuts at: 1391 2266 2391 3020 3557 1391
Size: 875 125 629 537 4072
Fragments arranged by size:
4072 875 629 537 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3138 3265 928
Size: 971 1239 127 3901
Fragments arranged by size:
3901 1239 971 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2112 6233 2112
Size: 4121 2117
BlpI GC'TnA_GC
Cuts at: 3251 3655 3251
Size: 404 5834
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2288 4299 5455 1790
Size: 476 22 2011 1156 2573
Fragments arranged by size:
2573 2011 1156 476 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 3969 4927
Size: 416 53 83 399 24 874 1678 958
Cuts at: 4927 442
Size: 1753
Fragments arranged by size:
1753 1678 958 874 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 3437 4898 1239
Size: 1109 1089 1461 2579
Fragments arranged by size:
2579 1461 1109 1089
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3004 3230 4363 5228 5526 3004
Size: 226 1133 865 298 3716
Fragments arranged by size:
3716 1133 865 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 4901 882
Size: 617 720 2682 2219
Fragments arranged by size:
2682 2219 720 617
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4045 4427 5062 275
Size: 53 83 186 936 2512 382 635 1451
Fragments arranged by size:
2512 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4671 5069 5343 5490 5901 4671
Size: 398 274 147 411 5008
Fragments arranged by size:
5008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 1178
Size: 30 165 30 786 30 5197
Fragments arranged by size:
5197 786 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3516 1186
Size: 2330 3908
BseYI C'CCAG_C
Cuts at: 2037 2727 5441 5937 2037
Size: 690 2714 496 2338
Fragments arranged by size:
2714 2338 690 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3268 1163
Size: 573 1532 4133
Fragments arranged by size:
4133 1532 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3410 3688 4449 5477 1540
Size: 275 186 1409 278 761 1028 2301
Fragments arranged by size:
2301 1409 1028 761 278 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 4299 4384 5455 2193
Size: 2106 85 1071 2976
Fragments arranged by size:
2976 2106 1071 85
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2266 2288 2391 3020 3557 4299
Size: 399 403 73 22 103 629 537 742
Cuts at: 4299 4384 5455 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 629 537 403 399 103
85 73 22
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 2787 3593 844
Size: 1239 704 806 3489
Fragments arranged by size:
3489 1239 806 704
BsrBI CCG'CTC
Cuts at: 1394 1823 3067 4125 1394
Size: 429 1244 1058 3507
Fragments arranged by size:
3507 1244 1058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 2685 3342 3348 4733 4907 65
Size: 1183 1158 279 657 6 1385 174 1396
Fragments arranged by size:
1396 1385 1183 1158 657 279 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 4882 5069 1700
Size: 110 3072 187 2869
Fragments arranged by size:
3072 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 3295 2431
Size: 864 5374
BstYI r'GATC_y
Cuts at: 1972 2112 3523 3568 3935 4320 4337 5789 5875
Size: 140 1411 45 367 385 17 1452 86
Cuts at: 5875 5887 6233 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1411 385 367 346 140 86
45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2043 2043
Size: 6238
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4747
Fragments arranged by size:
4747 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5499
Fragments arranged by size:
5499 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 2682 3754 4548 4568 2117
Size: 565 1072 794 20 3787
Fragments arranged by size:
3787 1072 794 565 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2623 2877 3685
Size: 54 1425 50 275 18 793 254 808
Cuts at: 3685 4574 6203 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 808 793 275 254 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2252 2450 2748 3098 4168 1353
Size: 899 198 298 350 1070 3423
Fragments arranged by size:
3423 1070 899 350 298 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2348 2772 3148 3437 4301 4898
Size: 393 491 225 424 376 289 864 597
Cuts at: 4898 5685 5953 1239
Size: 787 268 1524
Fragments arranged by size:
1524 864 787 597 491 424 393 376
289 268 225
EcoO109I rG'GnC_Cy
Cuts at: 2242 2262 2263 3457 2242
Size: 20 1 1194 5023
Fragments arranged by size:
5023 1194 20 1
EcoRI G'AATT_C
Cuts at: 2650 3547 3925 2650
Size: 897 378 4963
Fragments arranged by size:
4963 897 378
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 5385 1678
Size: 3707 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 2749 2781 3515
Size: 32 135 32 1079 32 66 32 734
Cuts at: 3515 3547 4879 4911 4953 4985 1373
Size: 32 1332 32 42 32 2626
Fragments arranged by size:
2626 1332 1079 734 135 66 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2011 3582 3828 669
Size: 988 281 73 1571 246 3079
Fragments arranged by size:
3079 1571 988 281 246 73
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 3451 3563 3451
Size: 112 6126
MfeI C'AATT_G
Cuts at: 3837 3837
Size: 6238
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5352 5536 1305
Size: 4047 184 2007
Fragments arranged by size:
4047 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2625 2879 10
Size: 54 1425 343 793 254 3369
Fragments arranged by size:
3369 1425 793 343 254 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 3050 3077 3389 4196 4555 4714
Size: 648 1635 249 27 312 807 359 159
Cuts at: 4714 518
Size: 2042
Fragments arranged by size:
2042 1635 807 648 359 312 249 159
27
MspA1I CmG'CkG
Cuts at: 1606 3654 4331 5479 5724 1606
Size: 2048 677 1148 245 2120
Fragments arranged by size:
2120 2048 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4747
Fragments arranged by size:
4747 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3596 5141 1132
Size: 97 2367 1545 2229
Fragments arranged by size:
2367 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2081 1348
Size: 733 5505
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6238
PfoI T'CCnGG_A
Cuts at: 2325 2325
Size: 6238
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3457 3457
Size: 6238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PspOMI G'GGCC_C
Cuts at: 2262 2262
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3481 3590 830
Size: 2651 109 3478
Fragments arranged by size:
3478 2651 109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SapI GCTCTTCn'nnn_
Cuts at: 3098 3098
Size: 6238
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SfcI C'TryA_G
Cuts at: 826 1045 3157 3477 3586 4721 5402 5593 826
Size: 219 2112 320 109 1135 681 191 1471
Fragments arranged by size:
2112 1471 1135 681 320 219 191 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3019 3245 4342 5243 5505 820
Size: 197 926 1076 226 1097 901 262 1553
Fragments arranged by size:
1553 1097 1076 926 901 262 226 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 2925 4162 5
Size: 47 2873 1237 2081
Fragments arranged by size:
2873 2081 1237 47
StuI AGG'CCT
Cuts at: 1517 2333 3363 1517
Size: 816 1030 4392
Fragments arranged by size:
4392 1030 816
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 3677 513
Size: 552 939 177 1496 3074
Fragments arranged by size:
3074 1496 939 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 2669 4265 4282 4435 4620 5065
Size: 1255 474 615 1596 17 153 185 445
Cuts at: 5065 325
Size: 1498
Fragments arranged by size:
1596 1498 1255 615 474 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 4484 6196
Size: 274 80 33 51 1443 723 1784 1712
Cuts at: 6196 96
Size: 138
Fragments arranged by size:
1784 1712 1443 723 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6198 6230 6198
Size: 32 6206
Tth111I GACn'n_nGTC
Cuts at: 1316 3237 1316
Size: 1921 4317
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2482 3295 2482
Size: 813 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 6238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI