Restriction map for
pFN21AE6402
(Circular) (Six-base) MAPSORT of: pFN21AE6402.seq Check: 3916 from: 1 to: 6310
pFN21AE6402 6310 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3589 3589
Size: 6310
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4120 278
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3653 1937
Size: 1716 4594
Acc65I G'GTAC_C
Cuts at: 3631 3631
Size: 6310
AclI AA'CG_TT
Cuts at: 1563 4437 4810 1563
Size: 2874 373 3063
Fragments arranged by size:
3063 2874 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2277 2414 2427 3460 4373 5757 6025
Size: 462 183 137 13 1033 913 1384 268
Cuts at: 6025 1632
Size: 1917
Fragments arranged by size:
1917 1384 1033 913 462 268 183 137
13
AfeI AGC'GCT
Cuts at: 2951 2951
Size: 6310
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6113
AflIII A'CryG_T
Cuts at: 1203 5209 6186 1203
Size: 4006 977 1327
Fragments arranged by size:
4006 1327 977
AgeI A'CCGG_T
Cuts at: 5141 5141
Size: 6310
AhdI GACnn_n'nnGTC
Cuts at: 5039 5039
Size: 6310
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6310
AlwNI CAG_nnn'CTG
Cuts at: 4053 5625 4053
Size: 1572 4738
ApaLI G'TGCA_C
Cuts at: 4367 5523 4367
Size: 1156 5154
ApoI r'AATT_y
Cuts at: 1493 2144 2740 3619 3845 3997 6106 1493
Size: 651 596 879 226 152 2109 1697
Fragments arranged by size:
2109 1697 879 651 596 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4864 160
Size: 872 3832 1606
Fragments arranged by size:
3832 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
AvaI C'yCGr_G
Cuts at: 1870 1943 3635 1870
Size: 73 1692 4545
Fragments arranged by size:
4545 1692 73
BamHI G'GATC_C
Cuts at: 2043 2786 3640 4007 2043
Size: 743 854 367 4346
Fragments arranged by size:
4346 854 743 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2690 3631 5086 618
Size: 325 217 627 903 941 1455 1842
Fragments arranged by size:
1842 1455 941 903 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2041 3629 1391
Size: 650 1588 4072
Fragments arranged by size:
4072 1588 650
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2396 928
Size: 971 497 4842
Fragments arranged by size:
4842 971 497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4501 4535 1903
Size: 34 2564 34 3678
Fragments arranged by size:
3678 2564 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4202 5412 4202
Size: 1210 5100
BclI T'GATC_A
Cuts at: 1548 3078 1548
Size: 1530 4780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2231 4921
Size: 107 122 71 631 605 337 222 2690
Cuts at: 4921 136
Size: 1525
Fragments arranged by size:
2690 1525 631 605 337 222 122 107
71
BglII A'GATC_T
Cuts at: 6305 6305
Size: 6310
BlpI GC'TnA_GC
Cuts at: 3727 3727
Size: 6310
Bme1580I G_kGCm'C
Cuts at: 1790 2695 4371 5527 1790
Size: 905 1676 1156 2573
Fragments arranged by size:
2573 1676 1156 905
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6310
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4041 4999 442
Size: 416 53 83 399 24 2624 958 1753
Fragments arranged by size:
2624 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 2670 3078 3302 4970 1239
Size: 1424 7 408 224 1668 2579
Fragments arranged by size:
2579 1668 1424 408 224 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4435 5300 5598 4435
Size: 865 298 5147
Fragments arranged by size:
5147 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2471 4973 882
Size: 617 972 2502 2219
Fragments arranged by size:
2502 2219 972 617
BsaAI yAC'GTr
Cuts at: 493 1204 4101 493
Size: 711 2897 2702
Fragments arranged by size:
2897 2702 711
BsaBI GATnn'nnATC
Cuts at: 2177 6304 2177
Size: 4127 2183
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4117 4499 5134 275
Size: 53 83 186 936 2584 382 635 1451
Fragments arranged by size:
2584 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2758 4743 5141 5415 5562 5973 2758
Size: 1985 398 274 147 411 3095
Fragments arranged by size:
3095 1985 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3405 3435 1178
Size: 30 165 30 2002 30 4053
Fragments arranged by size:
4053 2002 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3266 1186
Size: 2080 4230
BseYI C'CCAG_C
Cuts at: 2188 5513 6009 2188
Size: 3325 496 2489
Fragments arranged by size:
3325 2489 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2562 1163
Size: 573 826 4911
Fragments arranged by size:
4911 826 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3760 4521 5549 1540
Size: 275 186 1759 761 1028 2301
Fragments arranged by size:
2301 1759 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4371 4456 5527 4371
Size: 85 1071 5154
Fragments arranged by size:
5154 1071 85
BsmI GAATG_Cn'
Cuts at: 3821 3914 3821
Size: 93 6217
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2695 3629 4371 4456 5527 1391
Size: 399 251 654 934 742 85 1071 2174
Fragments arranged by size:
2174 1071 934 742 654 399 251 85
BspEI T'CCGG_A
Cuts at: 2758 5973 2758
Size: 3215 3095
BspHI T'CATG_A
Cuts at: 2466 4199 5929 2466
Size: 1733 1730 2847
Fragments arranged by size:
2847 1733 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2041 2302 3589 3665 844
Size: 1197 261 1287 76 3489
Fragments arranged by size:
3489 1287 1197 261 76
BsrBI CCG'CTC
Cuts at: 1394 1823 4197 1394
Size: 429 2374 3507
Fragments arranged by size:
3507 2374 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2250 2547 4805 4979 65
Size: 1183 1002 297 2258 174 1396
Fragments arranged by size:
2258 1396 1183 1002 297 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3458 4954 5141 1700
Size: 110 1648 1496 187 2869
Fragments arranged by size:
2869 1648 1496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6310
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6310
BssSI C'ACGA_G
Cuts at: 4370 5382 4370
Size: 1012 5298
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2252 2317 2549 794
Size: 1458 65 232 4555
Fragments arranged by size:
4555 1458 232 65
BstBI TT'CG_AA
Cuts at: 5114 5114
Size: 6310
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6310
BstXI CCAn_nnnn'nTGG
Cuts at: 2503 2602 2687 2921 2503
Size: 99 85 234 5892
Fragments arranged by size:
5892 234 99 85
BstYI r'GATC_y
Cuts at: 1972 2043 2786 3640 4007 4392 4409 5861 5947
Size: 71 743 854 367 385 17 1452 86
Cuts at: 5947 5959 6305 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 854 743 385 367 346 86
71 17 12
Bsu36I CC'TnA_GG
Cuts at: 2220 2220
Size: 6310
BtgI C'CryG_G
Cuts at: 513 1065 2004 2595 2601 2719 2869 3600 513
Size: 552 939 591 6 118 150 731 3223
Fragments arranged by size:
3223 939 731 591 552 150 118 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5571
Fragments arranged by size:
5571 683 56
BtsI GCAGTG_nn'
Cuts at: 2037 3369 3826 4620 4640 2037
Size: 1332 457 794 20 3707
Fragments arranged by size:
3707 1332 794 457 20
ClaI AT'CG_AT
Cuts at: 1554 2175 1554
Size: 621 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6275
DraI TTT'AAA
Cuts at: 3614 3614
Size: 6310
DraIII CAC_nnn'GTG
Cuts at: 3394 3394
Size: 6310
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5317 809
Size: 507 4001 1802
Fragments arranged by size:
4001 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 3757 4646
Size: 54 1425 50 275 18 768 1159 889
Cuts at: 4646 6275 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1159 889 768 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3757 1537
Size: 275 1945 4090
Fragments arranged by size:
4090 1945 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2110 2135 4240 1353
Size: 757 25 2105 3423
Fragments arranged by size:
3423 2105 757 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4897 5271 5417 387
Size: 1064 138 3308 374 146 1280
Fragments arranged by size:
3308 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2277 2414 2427 2663 2670 3078
Size: 393 462 183 137 13 236 7 408
Cuts at: 3078 3302 3460 4373 4970 5757 6025 1239
Size: 224 158 913 597 787 268 1524
Fragments arranged by size:
1524 913 787 597 462 408 393 268
236 224 183 158 137 13 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3475 3475
Size: 6310
EcoO109I rG'GnC_Cy
Cuts at: 2215 2215
Size: 6310
EcoRI G'AATT_C
Cuts at: 3619 3997 3619
Size: 378 5932
EcoRV GAT'ATC
Cuts at: 2179 2179
Size: 6310
FspI TGC'GCA
Cuts at: 4816 4816
Size: 6310
HaeII r_GCGC'y
Cuts at: 1678 2015 2953 5457 1678
Size: 337 938 2504 2531
Fragments arranged by size:
2531 2504 938 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 2793 2807 2825
Size: 32 135 32 456 32 733 14 18
Cuts at: 2825 2839 3129 3161 3195 3227 3247 3279 3405
Size: 14 290 32 34 32 20 32 126
Cuts at: 3405 3437 4951 4983 5025 5057 1373
Size: 32 1514 32 42 32 2626
Fragments arranged by size:
2626 1514 733 456 290 135 126 42
34 32 32 32 32 32 32 32
32 32 20 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3654 3900 669
Size: 988 281 1716 246 3079
Fragments arranged by size:
3079 1716 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6310
HpaI GTT'AAC
Cuts at: 3900 3900
Size: 6310
KpnI G_GTAC'C
Cuts at: 3635 3635
Size: 6310
MfeI C'AATT_G
Cuts at: 3909 3909
Size: 6310
MluI A'CGCG_T
Cuts at: 6186 6186
Size: 6310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2814 3181 3382 5424 5608 1305
Size: 1509 367 201 2042 184 2007
Fragments arranged by size:
2042 2007 1509 367 201 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 10
Size: 54 1425 343 768 3720
Fragments arranged by size:
3720 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 3431 4268 4627 4786 518
Size: 648 1434 831 837 359 159 2042
Fragments arranged by size:
2042 1434 837 831 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2917 3726 4403 5551 5796 1606
Size: 1311 809 677 1148 245 2120
Fragments arranged by size:
2120 1311 1148 809 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2595 2601 2719 2869 3600 513
Size: 552 939 591 6 118 150 731 3223
Fragments arranged by size:
3223 939 731 591 552 150 118 6
NdeI CA'TA_TG
Cuts at: 387 3331 387
Size: 2944 3366
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6310
NotI GC'GGCC_GC
Cuts at: 3757 3757
Size: 6310
NspI r_CATG'y
Cuts at: 1132 1229 2447 3023 3668 5213 1132
Size: 97 1218 576 645 1545 2229
Fragments arranged by size:
2229 1545 1218 645 576 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6310
PciI A'CATG_T
Cuts at: 5209 5209
Size: 6310
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2997 3394 1108
Size: 1889 397 4024
Fragments arranged by size:
4024 1889 397
PfoI T'CCnGG_A
Cuts at: 2679 2679
Size: 6310
PmeI GTTT'AAAC
Cuts at: 3614 3614
Size: 6310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4379 4411 5892 5924 4379
Size: 32 1481 32 4765
Fragments arranged by size:
4765 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 2215
Size: 6310
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6310
PsiI TTA'TAA
Cuts at: 3880 3880
Size: 6310
PstI C_TGCA'G
Cuts at: 830 2052 3662 830
Size: 1222 1610 3478
Fragments arranged by size:
3478 1610 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6310
PvuII CAG'CTG
Cuts at: 2917 2917
Size: 6310
SalI G'TCGA_C
Cuts at: 1936 3652 1936
Size: 1716 4594
SanDI GG'GwC_CC
Cuts at: 2215 2215
Size: 6310
SapI GCTCTTCn'nnn_
Cuts at: 2135 2135
Size: 6310
SbfI CC_TGCA'GG
Cuts at: 2052 3662 2052
Size: 1610 4700
ScaI AGT'ACT
Cuts at: 4558 6270 4558
Size: 1712 4598
SexAI A'CCwGG_T
Cuts at: 2081 2081
Size: 6310
SfcI C'TryA_G
Cuts at: 826 1045 2048 2449 3658 4793 5474 5665 826
Size: 219 1003 401 1209 1135 681 191 1471
Fragments arranged by size:
1471 1209 1135 1003 681 401 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6310
SmaI CCC'GGG
Cuts at: 1872 3637 1872
Size: 1765 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4414 5315 5577 820
Size: 197 926 2471 901 262 1553
Fragments arranged by size:
2471 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4101 493
Size: 3608 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6310
SphI G_CATG'C
Cuts at: 1132 2447 3023 3668 1132
Size: 1315 576 645 3774
Fragments arranged by size:
3774 1315 645 576
SspI AAT'ATT
Cuts at: 5 52 4234 5
Size: 47 4182 2081
Fragments arranged by size:
4182 2081 47
StuI AGG'CCT
Cuts at: 1517 2427 1517
Size: 910 5400
StyI C'CwwG_G
Cuts at: 513 1065 2004 2061 2229 2595 2601 2719 2869
Size: 552 939 57 168 366 6 118 150
Cuts at: 2869 3600 3749 513
Size: 731 149 3074
Fragments arranged by size:
3074 939 731 552 366 168 150 149
118 57 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4337 4354 4507 4692 5137 325
Size: 1255 2757 17 153 185 445 1498
Fragments arranged by size:
2757 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4556 6268 96
Size: 274 80 33 51 1443 2579 1712 138
Fragments arranged by size:
2579 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6270 6302 6270
Size: 32 6278
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6310
XbaI T'CTAG_A
Cuts at: 3646 3646
Size: 6310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 2236
Size: 6310
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6310
XmaI C'CCGG_G
Cuts at: 1870 3635 1870
Size: 1765 4545
XmnI GAAnn'nnTTC
Cuts at: 4439 4439
Size: 6310
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4118 276
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI
SfoI SrfI SwaI