Restriction map for
pFN21AE6401
(Circular) (Six-base) MAPSORT of: pFN21AE6401.seq Check: 8004 from: 1 to: 6136
pFN21AE6401 6136 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2433 3479 1937
Size: 496 1046 4594
Fragments arranged by size:
4594 1046 496
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3337 4199 5583 5851 1632
Size: 1705 862 1384 268 1917
Fragments arranged by size:
1917 1705 1384 862 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 2361 5035 6012 1203
Size: 1158 2674 977 1327
Fragments arranged by size:
2674 1327 1158 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 2924 1166
Size: 1758 4378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AlwNI CAG_nnn'CTG
Cuts at: 2809 2914 3879 5451 2809
Size: 105 965 1572 3494
Fragments arranged by size:
3494 1572 965 105
ApaI G_GGCC'C
Cuts at: 2114 3177 2114
Size: 1063 5073
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 3445 3671 3823 5932 1493
Size: 1952 226 152 2109 1697
Fragments arranged by size:
2109 1952 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2612 3461 1870
Size: 73 69 600 849 4545
Fragments arranged by size:
4545 849 600 73 69
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2240 2646 3014 3073 3457
Size: 325 217 627 453 406 368 59 384
Cuts at: 3457 4912 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 453 406 384 368 325
217 59
BanII G_rGCy'C
Cuts at: 1391 2114 2687 3177 3455 1391
Size: 723 573 490 278 4072
Fragments arranged by size:
4072 723 573 490 278
BbeI G_GCGC'C
Cuts at: 2650 3018 3077 2650
Size: 368 59 5709
Fragments arranged by size:
5709 368 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 2709 928
Size: 971 175 635 4355
Fragments arranged by size:
4355 971 635 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4327 4361 1903
Size: 34 2390 34 3678
Fragments arranged by size:
3678 2390 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2525 3071 3122
Size: 107 122 71 631 605 853 546 51
Cuts at: 3122 4747 136
Size: 1625 1525
Fragments arranged by size:
1625 1525 853 631 605 546 122 107
71 51
BglII A'GATC_T
Cuts at: 3201 6131 3201
Size: 2930 3206
BlpI GC'TnA_GC
Cuts at: 2297 2721 3553 2297
Size: 424 832 4880
Fragments arranged by size:
4880 832 424
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2322 2577 3177 4197 5353 1790
Size: 324 208 255 600 1020 1156 2573
Fragments arranged by size:
2573 1156 1020 600 324 255 208
BmgBI CAC'GTC
Cuts at: 1318 2362 1318
Size: 1044 5092
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4825 442
Size: 416 53 83 399 24 2450 958 1753
Fragments arranged by size:
2450 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2561 4796 1239
Size: 1322 2235 2579
Fragments arranged by size:
2579 2235 1322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4261 5126 5424 4261
Size: 865 298 4973
Fragments arranged by size:
4973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2609 2837 4799 882
Size: 617 1110 228 1962 2219
Fragments arranged by size:
2219 1962 1110 617 228
BsaAI yAC'GTr
Cuts at: 493 1204 3061 3927 493
Size: 711 1857 866 2702
Fragments arranged by size:
2702 1857 866 711
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2202 2647 2784
Size: 53 83 186 936 476 193 445 137
Cuts at: 2784 3015 3074 3943 4325 4960 275
Size: 231 59 869 382 635 1451
Fragments arranged by size:
1451 936 869 635 476 445 382 231
193 186 137 83 59 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5911
Fragments arranged by size:
5911 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 3043 3179 1186
Size: 1192 665 136 4143
Fragments arranged by size:
4143 1192 665 136
BseYI C'CCAG_C
Cuts at: 2031 2260 2374 2455 2532 2563 2697 3168 5339
Size: 229 114 81 77 31 134 471 2171
Cuts at: 5339 5835 2031
Size: 496 2332
Fragments arranged by size:
2332 2171 496 471 229 134 114 81
77 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 3069 1163
Size: 573 771 562 4230
Fragments arranged by size:
4230 771 573 562
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2074 2892 3586 4347 5375 1540
Size: 275 186 73 818 694 761 1028 2301
Fragments arranged by size:
2301 1028 818 761 694 275 186 73
BsiHKAI G_wGCw'C
Cuts at: 2315 4197 4282 5353 2315
Size: 1882 85 1071 3098
Fragments arranged by size:
3098 1882 1071 85
BsmI GAATG_Cn'
Cuts at: 3647 3740 3647
Size: 93 6043
BsmBI CGTCTCn'nnnn_
Cuts at: 2791 2791
Size: 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2315 2322 2577 2687 3177 3455
Size: 399 324 201 7 255 110 490 278
Cuts at: 3455 4197 4282 5353 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 324 278 255
201 110 85 7
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3491 844
Size: 2647 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4023 1394
Size: 429 2200 3507
Fragments arranged by size:
3507 2200 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2330 2393 3152 4780 4967 1700
Size: 110 520 63 759 1628 187 2869
Fragments arranged by size:
2869 1628 759 520 187 110 63
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 2061 2170 1925
Size: 136 109 5891
Fragments arranged by size:
5891 136 109
BssSI C'ACGA_G
Cuts at: 3001 3272 4196 5208 3001
Size: 271 924 1012 3929
Fragments arranged by size:
3929 1012 924 271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3138 794
Size: 2344 3792
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstYI r'GATC_y
Cuts at: 1972 3201 3466 3833 4218 4235 5687 5773 5785
Size: 1229 265 367 385 17 1452 86 12
Cuts at: 5785 6131 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1229 385 367 346 265 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3308 3308
Size: 6136
BtgI C'CryG_G
Cuts at: 513 1065 2068 2244 2349 2658 3126 513
Size: 552 1003 176 105 309 468 3523
Fragments arranged by size:
3523 1003 552 468 309 176 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3133 487
Size: 683 56 1907 3490
Fragments arranged by size:
3490 1907 683 56
BtsI GCAGTG_nn'
Cuts at: 3652 4446 4466 3652
Size: 794 20 5322
Fragments arranged by size:
5322 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2151 2328
Size: 54 1425 50 275 18 241 80 177
Cuts at: 2328 2661 3063 3123 3583 4472 6101 8
Size: 333 402 60 460 889 1629 43
Fragments arranged by size:
1629 1425 889 460 402 333 275 241
177 80 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2071 3583 1537
Size: 275 259 1512 4090
Fragments arranged by size:
4090 1512 275 259
EarI CTCTTCn'nnn_
Cuts at: 1353 2850 3149 4066 1353
Size: 1497 299 917 3423
Fragments arranged by size:
3423 1497 917 299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2971 4723 5097 5243 387
Size: 1064 138 1382 1752 374 146 1280
Fragments arranged by size:
1752 1382 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2561 3337 4199 4796 5583 5851 1239
Size: 393 929 776 862 597 787 268 1524
Fragments arranged by size:
1524 929 862 787 776 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3299 3299
Size: 6136
EcoO109I rG'GnC_Cy
Cuts at: 2017 2045 3173 3304 3312 2017
Size: 28 1128 131 8 4841
Fragments arranged by size:
4841 1128 131 28 8
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 2551 2650 3018 3077 5283 1678
Size: 873 99 368 59 2206 2531
Fragments arranged by size:
2531 2206 873 368 99 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3193 3225 4777 4809 4851
Size: 32 135 32 1621 32 1552 32 42
Cuts at: 4851 4883 1373
Size: 32 2626
Fragments arranged by size:
2626 1621 1552 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2821 3480 3726 669
Size: 988 281 883 659 246 3079
Fragments arranged by size:
3079 988 883 659 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KasI G'GCGC_C
Cuts at: 2646 3014 3073 2646
Size: 368 59 5709
Fragments arranged by size:
5709 368 59
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5250 5434 1305
Size: 3945 184 2007
Fragments arranged by size:
3945 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3065 10
Size: 54 1425 343 1233 3081
Fragments arranged by size:
3081 1425 1233 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2909 2924 4094 4453 4612 518
Size: 648 1743 15 1170 359 159 2042
Fragments arranged by size:
2042 1743 1170 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2070 2102 2246 2351 2809 2924 3552 4229
Size: 464 32 144 105 458 115 628 677
Cuts at: 4229 5377 5622 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 628 464 458 245 144
115 105 32
NaeI GCC'GGC
Cuts at: 2332 3154 2332
Size: 822 5314
NarI GG'CG_CC
Cuts at: 2647 3015 3074 2647
Size: 368 59 5709
Fragments arranged by size:
5709 368 59
NcoI C'CATG_G
Cuts at: 513 1065 2658 513
Size: 552 1593 3991
Fragments arranged by size:
3991 1593 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NgoMIV G'CCGG_C
Cuts at: 2330 3152 2330
Size: 822 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 2071 3583 2071
Size: 1512 4624
NspI r_CATG'y
Cuts at: 1132 1229 3139 3218 3494 5039 1132
Size: 97 1910 79 276 1545 2229
Fragments arranged by size:
2229 1910 1545 276 97 79
PasI CC'CwG_GG
Cuts at: 1348 2107 2236 3034 1348
Size: 759 129 798 4450
Fragments arranged by size:
4450 798 759 129
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3183 1108
Size: 2075 4061
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PmlI CAC'GTG
Cuts at: 3061 3061
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 4205 4237 5718 5750 2849
Size: 32 1324 32 1481 32 3235
Fragments arranged by size:
3235 1481 1324 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2017 3304 2017
Size: 1287 4849
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspOMI G'GGCC_C
Cuts at: 2110 3173 2110
Size: 1063 5073
PstI C_TGCA'G
Cuts at: 830 2169 3488 830
Size: 1339 1319 3478
Fragments arranged by size:
3478 1339 1319
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2809 2924 2809
Size: 115 6021
SacII CC_GC'GG
Cuts at: 2071 2247 2352 2071
Size: 176 105 5855
Fragments arranged by size:
5855 176 105
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 2140 2165 3484 4619 5300 5491 826
Size: 219 1095 25 1319 1135 681 191 1471
Fragments arranged by size:
1471 1319 1135 1095 681 219 191 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 3122 3122
Size: 6136
SfoI GGC'GCC
Cuts at: 2648 3016 3075 2648
Size: 368 59 5709
Fragments arranged by size:
5709 368 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4240 5141 5403 820
Size: 197 926 2297 901 262 1553
Fragments arranged by size:
2297 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2658 3279 3575 513
Size: 552 1593 621 296 3074
Fragments arranged by size:
3074 1593 621 552 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2228 2524 2878 2888 4163 4180 4333
Size: 1255 648 296 354 10 1275 17 153
Cuts at: 4333 4518 4963 325
Size: 185 445 1498
Fragments arranged by size:
1498 1275 1255 648 445 354 296 185
153 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3041 3109 3259 3041
Size: 68 150 5918
Fragments arranged by size:
5918 150 68
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 4265 4265
Size: 6136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI