Restriction map for
pFN21AE6393
(Circular) (Six-base) MAPSORT of: pFN21AE6393.seq Check: 9773 from: 1 to: 6019
pFN21AE6393 6019 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3829 278
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2499 2949 3362 1937
Size: 562 450 413 4594
Fragments arranged by size:
4594 562 450 413
Acc65I G'GTAC_C
Cuts at: 3340 3340
Size: 6019
AclI AA'CG_TT
Cuts at: 1563 2979 4146 4519 1563
Size: 1416 1167 373 3063
Fragments arranged by size:
3063 1416 1167 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4082 5466 5734 1632
Size: 2450 1384 268 1917
Fragments arranged by size:
2450 1917 1384 268
AfeI AGC'GCT
Cuts at: 2414 2414
Size: 6019
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5822
AflIII A'CryG_T
Cuts at: 1203 2757 4918 5895 1203
Size: 1554 2161 977 1327
Fragments arranged by size:
2161 1554 1327 977
AgeI A'CCGG_T
Cuts at: 4850 4850
Size: 6019
AhdI GACnn_n'nnGTC
Cuts at: 4748 4748
Size: 6019
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2876 3266 1184
Size: 1692 390 3937
Fragments arranged by size:
3937 1692 390
AlwNI CAG_nnn'CTG
Cuts at: 2081 2242 2389 3762 5334 2081
Size: 161 147 1373 1572 2766
Fragments arranged by size:
2766 1572 1373 161 147
ApaI G_GGCC'C
Cuts at: 2124 2203 2653 2923 3113 2124
Size: 79 450 270 190 5030
Fragments arranged by size:
5030 450 270 190 79
ApaLI G'TGCA_C
Cuts at: 2294 2837 4076 5232 2294
Size: 543 1239 1156 3081
Fragments arranged by size:
3081 1239 1156 543
ApoI r'AATT_y
Cuts at: 1493 3328 3554 3706 5815 1493
Size: 1835 226 152 2109 1697
Fragments arranged by size:
2109 1835 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4573 160
Size: 872 3541 1606
Fragments arranged by size:
3541 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2463 2687 2720 3106 3344 1870
Size: 73 306 214 224 33 386 238 4545
Fragments arranged by size:
4545 386 306 238 224 214 73 33
AvrII C'CTAG_G
Cuts at: 2491 2491
Size: 6019
BamHI G'GATC_C
Cuts at: 3349 3716 3349
Size: 367 5652
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2724 2821 3242 3296
Size: 325 217 627 273 664 97 421 54
Cuts at: 3296 3340 4795 618
Size: 44 1455 1842
Fragments arranged by size:
1842 1455 664 627 421 325 273 217
97 54 44
BanII G_rGCy'C
Cuts at: 1391 2124 2203 2653 2705 2923 3056 3113 3338
Size: 733 79 450 52 218 133 57 225
Cuts at: 3338 1391
Size: 4072
Fragments arranged by size:
4072 733 450 225 218 133 79 57
52
BbeI G_GCGC'C
Cuts at: 3300 3300
Size: 6019
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 3256 3268 928
Size: 971 126 1231 12 3679
Fragments arranged by size:
3679 1231 971 126 12
BbvCI CC'TCA_GC
Cuts at: 3023 3023
Size: 6019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4210 4244 1903
Size: 34 2273 34 3678
Fragments arranged by size:
3678 2273 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3911 5121 3911
Size: 1210 4809
BclI T'GATC_A
Cuts at: 1548 2400 1548
Size: 852 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 4630 136
Size: 107 122 71 631 605 500 2458 1525
Fragments arranged by size:
2458 1525 631 605 500 122 107 71
BglII A'GATC_T
Cuts at: 6014 6014
Size: 6019
BlpI GC'TnA_GC
Cuts at: 3436 3436
Size: 6019
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2124 2203 2298 2458 2653 2727 2734
Size: 281 53 79 95 160 195 74 7
Cuts at: 2734 2826 2841 2923 3113 4080 5236 1790
Size: 92 15 82 190 967 1156 2573
Fragments arranged by size:
2573 1156 967 281 195 190 160 95
92 82 79 74 53 15 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2059 3001 3750
Size: 416 53 83 399 24 642 942 749
Cuts at: 3750 4708 442
Size: 958 1753
Fragments arranged by size:
1753 958 942 749 642 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2401 2798 2849 4679 1239
Size: 824 338 397 51 1830 2579
Fragments arranged by size:
2579 1830 824 397 338 51
Bpu10I CC'TnA_GC
Cuts at: 2148 3023 2148
Size: 875 5144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4144 5009 5307 4144
Size: 865 298 4856
Fragments arranged by size:
4856 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4682 882
Size: 617 3183 2219
Fragments arranged by size:
3183 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 3810 493
Size: 711 1317 1289 2702
Fragments arranged by size:
2702 1317 1289 711
BsaBI GATnn'nnATC
Cuts at: 6013 6013
Size: 6019
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3297 3826 4208 4843
Size: 53 83 186 936 1764 529 382 635
Cuts at: 4843 275
Size: 1451
Fragments arranged by size:
1764 1451 936 635 529 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4452 4850 5124 5271 5682 4452
Size: 398 274 147 411 4789
Fragments arranged by size:
4789 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5794
Fragments arranged by size:
5794 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2218 3206 1186
Size: 865 167 988 3999
Fragments arranged by size:
3999 988 865 167
BseYI C'CCAG_C
Cuts at: 2139 2162 2176 2785 2996 3119 3292 5222 5718
Size: 23 14 609 211 123 173 1930 496
Cuts at: 5718 2139
Size: 2440
Fragments arranged by size:
2440 1930 609 496 211 173 123 23
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5446
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3469 4230 5258 1540
Size: 275 186 1468 761 1028 2301
Fragments arranged by size:
2301 1468 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 2841 2963 3056 4080 4165 5236 2298
Size: 543 122 93 1024 85 1071 3081
Fragments arranged by size:
3081 1071 1024 543 122 93 85
BsmI GAATG_Cn'
Cuts at: 3530 3623 3530
Size: 93 5926
BsmBI CGTCTCn'nnnn_
Cuts at: 2905 2905
Size: 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2124 2203 2298 2458 2653 2705
Size: 399 281 53 79 95 160 195 52
Cuts at: 2705 2727 2734 2826 2841 2923 2963 3056 3113
Size: 22 7 92 15 82 40 93 57
Cuts at: 3113 3338 4080 4165 5236 1391
Size: 225 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 281 225 195 160
95 93 92 85 82 79 57 53
52 40 22 15 7
BspEI T'CCGG_A
Cuts at: 5682 5682
Size: 6019
BspHI T'CATG_A
Cuts at: 2985 3908 5638 2985
Size: 923 1730 3366
Fragments arranged by size:
3366 1730 923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3297 3374 844
Size: 2453 77 3489
Fragments arranged by size:
3489 2453 77
BsrBI CCG'CTC
Cuts at: 1394 1823 3906 1394
Size: 429 2083 3507
Fragments arranged by size:
3507 2083 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2436 4514 4688 65
Size: 1183 1188 2078 174 1396
Fragments arranged by size:
2078 1396 1188 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4663 4850 1700
Size: 110 2853 187 2869
Fragments arranged by size:
2869 2853 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6019
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6019
BssSI C'ACGA_G
Cuts at: 4079 5091 4079
Size: 1012 5007
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2845 794
Size: 2051 3968
BstBI TT'CG_AA
Cuts at: 4823 4823
Size: 6019
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6019
BstXI CCAn_nnnn'nTGG
Cuts at: 2184 3127 2184
Size: 943 5076
BstYI r'GATC_y
Cuts at: 1972 2532 3349 3716 4101 4118 5570 5656 5668
Size: 560 817 367 385 17 1452 86 12
Cuts at: 5668 6014 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 817 560 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4528
Fragments arranged by size:
4528 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5280
Fragments arranged by size:
5280 683 56
BtsI GCAGTG_nn'
Cuts at: 2231 2456 3535 4329 4349 2231
Size: 225 1079 794 20 3901
Fragments arranged by size:
3901 1079 794 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5984
DraI TTT'AAA
Cuts at: 3323 3323
Size: 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5026 809
Size: 507 3710 1802
Fragments arranged by size:
3710 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2973 3466
Size: 54 1425 50 275 18 492 651 493
Cuts at: 3466 4355 5984 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 651 493 492 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3466 1537
Size: 275 1654 4090
Fragments arranged by size:
4090 1654 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2693 3260 3949 1353
Size: 1340 567 689 3423
Fragments arranged by size:
3423 1340 689 567
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4606 4980 5126 387
Size: 1064 138 3017 374 146 1280
Fragments arranged by size:
3017 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3054 3054
Size: 6019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2401 2798 2849 4082 4679 5466
Size: 393 431 338 397 51 1233 597 787
Cuts at: 5466 5734 1239
Size: 268 1524
Fragments arranged by size:
1524 1233 787 597 431 397 393 338
268 51
EcoO109I rG'GnC_Cy
Cuts at: 2053 2096 2120 2121 2649 2920 2053
Size: 43 24 1 528 271 5152
Fragments arranged by size:
5152 528 271 43 24 1
EcoRI G'AATT_C
Cuts at: 3328 3706 3328
Size: 378 5641
FspI TGC'GCA
Cuts at: 4525 4525
Size: 6019
HaeII r_GCGC'y
Cuts at: 1678 2416 2746 3199 3300 5166 1678
Size: 738 330 453 101 1866 2531
Fragments arranged by size:
2531 1866 738 453 330 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2992 3024 4660 4692 4734
Size: 32 135 32 1420 32 1636 32 42
Cuts at: 4734 4766 1373
Size: 32 2626
Fragments arranged by size:
2626 1636 1420 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3363 3609 669
Size: 988 281 1425 246 3079
Fragments arranged by size:
3079 1425 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6019
HpaI GTT'AAC
Cuts at: 3609 3609
Size: 6019
KasI G'GCGC_C
Cuts at: 3296 3296
Size: 6019
KpnI G_GTAC'C
Cuts at: 3344 3344
Size: 6019
MfeI C'AATT_G
Cuts at: 3618 3618
Size: 6019
MluI A'CGCG_T
Cuts at: 5895 5895
Size: 6019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5133 5317 1305
Size: 3828 184 2007
Fragments arranged by size:
3828 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2975 10
Size: 54 1425 343 1143 3054
Fragments arranged by size:
3054 1425 1143 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2339 2756 2762 2912 3977 4336
Size: 648 1135 38 417 6 150 1065 359
Cuts at: 4336 4495 518
Size: 159 2042
Fragments arranged by size:
2042 1135 1065 648 417 359 159 150
38 6
MspA1I CmG'CkG
Cuts at: 1606 2242 2452 2476 2559 2583 2785 2809 3097
Size: 636 210 24 83 24 202 24 288
Cuts at: 3097 3435 4112 5260 5505 1606
Size: 338 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 636 338 288 245 210
202 83 24 24 24
NarI GG'CG_CC
Cuts at: 3297 3297
Size: 6019
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4528
Fragments arranged by size:
4528 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6019
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6019
NotI GC'GGCC_GC
Cuts at: 3466 3466
Size: 6019
NspI r_CATG'y
Cuts at: 1132 1229 2338 2761 2917 3377 4922 1132
Size: 97 1109 423 156 460 1545 2229
Fragments arranged by size:
2229 1545 1109 460 423 156 97
PasI CC'CwG_GG
Cuts at: 1348 2100 1348
Size: 752 5267
PciI A'CATG_T
Cuts at: 2757 4918 2757
Size: 2161 3858
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2081 2389 1108
Size: 973 308 4738
Fragments arranged by size:
4738 973 308
PmeI GTTT'AAAC
Cuts at: 3323 3323
Size: 6019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 5601 5633 4088
Size: 32 1481 32 4474
Fragments arranged by size:
4474 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2096 2053
Size: 43 5976
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6019
PsiI TTA'TAA
Cuts at: 3589 3589
Size: 6019
PspOMI G'GGCC_C
Cuts at: 2120 2199 2649 2919 3109 2120
Size: 79 450 270 190 5030
Fragments arranged by size:
5030 450 270 190 79
PstI C_TGCA'G
Cuts at: 830 2238 2676 2808 3371 830
Size: 1408 438 132 563 3478
Fragments arranged by size:
3478 1408 563 438 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6019
PvuII CAG'CTG
Cuts at: 2242 2583 2785 2809 2242
Size: 341 202 24 5452
Fragments arranged by size:
5452 341 202 24
SacI G_AGCT'C
Cuts at: 3056 3056
Size: 6019
SalI G'TCGA_C
Cuts at: 1936 3361 1936
Size: 1425 4594
SanDI GG'GwC_CC
Cuts at: 2053 2096 2053
Size: 43 5976
SapI GCTCTTCn'nnn_
Cuts at: 2693 3260 2693
Size: 567 5452
SbfI CC_TGCA'GG
Cuts at: 3371 3371
Size: 6019
ScaI AGT'ACT
Cuts at: 4267 5979 4267
Size: 1712 4307
SfcI C'TryA_G
Cuts at: 826 1045 2234 2672 2804 3367 4502 5183 5374
Size: 219 1189 438 132 563 1135 681 191
Cuts at: 5374 826
Size: 1471
Fragments arranged by size:
1471 1189 1135 681 563 438 219 191
132
SfoI GGC'GCC
Cuts at: 3298 3298
Size: 6019
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6019
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6019
SmaI CCC'GGG
Cuts at: 1872 2465 2722 3346 1872
Size: 593 257 624 4545
Fragments arranged by size:
4545 624 593 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4123 5024 5286 820
Size: 197 926 2180 901 262 1553
Fragments arranged by size:
2180 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3810 493
Size: 3317 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6019
SphI G_CATG'C
Cuts at: 1132 3377 1132
Size: 2245 3774
SspI AAT'ATT
Cuts at: 5 52 3943 5
Size: 47 3891 2081
Fragments arranged by size:
3891 2081 47
StuI AGG'CCT
Cuts at: 1517 2213 2490 1517
Size: 696 277 5046
Fragments arranged by size:
5046 696 277
StyI C'CwwG_G
Cuts at: 513 1065 2004 2214 2491 3039 3458 513
Size: 552 939 210 277 548 419 3074
Fragments arranged by size:
3074 939 552 548 419 277 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2599 2656 3211 4046 4063 4216 4401
Size: 1255 1019 57 555 835 17 153 185
Cuts at: 4401 4846 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1019 835 555 445 185 153
57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2691 4265 5977
Size: 274 80 33 51 1443 714 1574 1712
Cuts at: 5977 96
Size: 138
Fragments arranged by size:
1712 1574 1443 714 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5979 6011 5979
Size: 32 5987
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6019
XbaI T'CTAG_A
Cuts at: 3355 3355
Size: 6019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6019
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6019
XmaI C'CCGG_G
Cuts at: 1870 2463 2720 3344 1870
Size: 593 257 624 4545
Fragments arranged by size:
4545 624 593 257
XmnI GAAnn'nnTTC
Cuts at: 3275 4148 3275
Size: 873 5146
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3827 276
Size: 53 83 186 936 2293 2468
Fragments arranged by size:
2468 2293 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacII SexAI SfiI SrfI SwaI