Restriction map for
pFN21AE6390
(Circular) (Six-base) MAPSORT of: pFN21AE6390.seq Check: 9523 from: 1 to: 5959
pFN21AE6390 5959 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AfeI AGC'GCT
Cuts at: 3250 3250
Size: 5959
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2177 2356 2926 3702 5274 2177
Size: 179 570 776 1572 2862
Fragments arranged by size:
2862 1572 776 570 179
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2015 2058 2959 3268 3494 3646 5755 1493
Size: 522 43 901 309 226 152 2109 1697
Fragments arranged by size:
2109 1697 901 522 309 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3073 3289 3656 3073
Size: 216 367 5376
Fragments arranged by size:
5376 367 216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3059 3280 4735 618
Size: 325 217 627 1272 221 1455 1842
Fragments arranged by size:
1842 1455 1272 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2350 3278 1391
Size: 959 928 4072
Fragments arranged by size:
4072 959 928
BbeI G_GCGC'C
Cuts at: 3063 3063
Size: 5959
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BbvCI CC'TCA_GC
Cuts at: 3160 3160
Size: 5959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2367 2454 1548
Size: 819 87 5053
Fragments arranged by size:
5053 819 87
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3008 3690 4648
Size: 416 53 83 399 24 1591 682 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1753 1591 958 682 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 2867 4619 1239
Size: 1071 557 1752 2579
Fragments arranged by size:
2579 1752 1071 557
Bpu10I CC'TnA_GC
Cuts at: 3160 3160
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 4622 882
Size: 617 1065 2058 2219
Fragments arranged by size:
2219 2058 1065 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3060 3766 4148 4783
Size: 53 83 186 936 1527 706 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1527 1451 936 706 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2068 4392 4790 5064 5211 5622 2068
Size: 2324 398 274 147 411 2405
Fragments arranged by size:
2405 2324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3033 1186
Size: 1847 4112
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2064 2658 1163
Size: 573 328 594 4464
Fragments arranged by size:
4464 594 573 328
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4020 4105 5176 4020
Size: 85 1071 4803
Fragments arranged by size:
4803 1071 85
BsmI GAATG_Cn'
Cuts at: 3122 3470 3563 3122
Size: 348 93 5518
Fragments arranged by size:
5518 348 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2350 3278 4020 4105 5176 1391
Size: 399 560 928 742 85 1071 2174
Fragments arranged by size:
2174 1071 928 742 560 399 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2400 2730 3235 3848 5578 2400
Size: 330 505 613 1730 2781
Fragments arranged by size:
2781 1730 613 505 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3257 3846 1394
Size: 429 1434 589 3507
Fragments arranged by size:
3507 1434 589 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2739 4454 4628 65
Size: 1183 1491 1715 174 1396
Fragments arranged by size:
1715 1491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2145 2182 3073 3289 3656 4041 4058 5510
Size: 173 37 891 216 367 385 17 1452
Cuts at: 5510 5596 5608 5954 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 891 385 367 346 216 173
86 37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3054 513
Size: 552 939 1050 3418
Fragments arranged by size:
3418 1050 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2664 3406 4295
Size: 54 1425 50 275 18 834 742 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 834 742 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2858 3889 1353
Size: 773 732 1031 3423
Fragments arranged by size:
3423 1031 773 732
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2867 4022 4619 5406 5674 1239
Size: 393 678 557 1155 597 787 268 1524
Fragments arranged by size:
1524 1155 787 678 597 557 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2419 2996 2419
Size: 577 5382
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 3063 3252 5106 1678
Size: 1385 189 1854 2531
Fragments arranged by size:
2531 1854 1385 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2315 4600 4632 4674
Size: 32 135 32 711 32 2285 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 2285 711 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KasI G'GCGC_C
Cuts at: 3059 3059
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 2253 3558 2253
Size: 1305 4654
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2290 2889 2925 2998 5073 5257 1305
Size: 985 599 36 73 2075 184 2007
Fragments arranged by size:
2075 2007 985 599 184 73 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2666 10
Size: 54 1425 343 834 3303
Fragments arranged by size:
3303 1425 834 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3104 3917 4276 4435 518
Size: 648 1938 813 359 159 2042
Fragments arranged by size:
2042 1938 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3184 3375 4052 5200 5445 1606
Size: 1578 191 677 1148 245 2120
Fragments arranged by size:
2120 1578 1148 677 245 191
NarI GG'CG_CC
Cuts at: 3060 3060
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 2004 3054 513
Size: 552 939 1050 3418
Fragments arranged by size:
3418 1050 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2156 2580 2662 1108
Size: 1048 424 82 4405
Fragments arranged by size:
4405 1048 424 82
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2082 2114 4028 4060 5541 5573 2082
Size: 32 1914 32 1481 32 2468
Fragments arranged by size:
2468 1914 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 2996 2419
Size: 577 5382
PshAI GACnn'nnGTC
Cuts at: 1613 2576 1613
Size: 963 4996
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2182 2428 3311 830
Size: 1352 246 883 3478
Fragments arranged by size:
3478 1352 883 246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 3184 3184
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SanDI GG'GwC_CC
Cuts at: 2419 2419
Size: 5959
SapI GCTCTTCn'nnn_
Cuts at: 2126 2126
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2178 2424 2510 2745 3307 4442 5123
Size: 219 1133 246 86 235 562 1135 681
Cuts at: 5123 5314 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1133 681 562 246 235 219
191 86
SfoI GGC'GCC
Cuts at: 3061 3061
Size: 5959
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2588 3024 1517
Size: 1071 436 4452
Fragments arranged by size:
4452 1071 436
StyI C'CwwG_G
Cuts at: 513 1065 2004 2779 3054 3398 513
Size: 552 939 775 275 344 3074
Fragments arranged by size:
3074 939 775 552 344 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 3986 4003 4156 4341 4786 325
Size: 1255 843 1563 17 153 185 445 1498
Fragments arranged by size:
1563 1498 1255 843 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 4205 5917
Size: 274 80 33 51 1443 852 1376 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1443 1376 852 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 2681 1316
Size: 1365 4594
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2357 2357
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SexAI SfiI SrfI SwaI