Restriction map for
pFN21AE6389
(Circular) (Six-base) MAPSORT of: pFN21AE6389.seq Check: 8654 from: 1 to: 5860
pFN21AE6389 5860 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2658 3151 3203 1937
Size: 721 493 52 4594
Fragments arranged by size:
4594 721 493 52
Acc65I G'GTAC_C
Cuts at: 3134 3181 3134
Size: 47 5813
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2370 3923 5307 5575 1632
Size: 431 307 1553 1384 268 1917
Fragments arranged by size:
1917 1553 1384 431 307 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 2348 3603 5175 2348
Size: 1255 1572 3033
Fragments arranged by size:
3033 1572 1255
ApaI G_GGCC'C
Cuts at: 2480 2480
Size: 5860
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2415 3185 1870
Size: 73 472 770 4545
Fragments arranged by size:
4545 770 472 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 3059 3134 3181 4636
Size: 325 217 627 341 931 75 47 1455
Cuts at: 4636 618
Size: 1842
Fragments arranged by size:
1842 1455 931 627 341 325 217 75
47
BanII G_rGCy'C
Cuts at: 1391 2480 2737 2967 3179 1391
Size: 1089 257 230 212 4072
Fragments arranged by size:
4072 1089 257 230 212
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4051 4085 1903
Size: 34 50 34 2030 34 3678
Fragments arranged by size:
3678 2030 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2801 4471 136
Size: 107 122 71 631 605 1129 1670 1525
Fragments arranged by size:
1670 1525 1129 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2480 3921 5077 1790
Size: 352 338 1441 1156 2573
Fragments arranged by size:
2573 1441 1156 352 338
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3591 4549 442
Size: 416 53 83 399 24 2174 958 1753
Fragments arranged by size:
2174 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2807 2981 4520 1239
Size: 1086 482 174 1539 2579
Fragments arranged by size:
2579 1539 1086 482 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2010 4293 4691 4965 5112 5523 2010
Size: 2283 398 274 147 411 2347
Fragments arranged by size:
2347 2283 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5860
BseYI C'CCAG_C
Cuts at: 2031 3016 5063 5559 2031
Size: 985 2047 496 2332
Fragments arranged by size:
2332 2047 985 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 2760 1163
Size: 573 435 589 4263
Fragments arranged by size:
4263 589 573 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2737 2821 3002 3921 4006 5077 2737
Size: 84 181 919 85 1071 3520
Fragments arranged by size:
3520 1071 919 181 85 84
BsmI GAATG_Cn'
Cuts at: 2649 3371 3464 2649
Size: 722 93 5045
Fragments arranged by size:
5045 722 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2480 2737 2821 2967 3002 3179
Size: 399 352 338 257 84 146 35 177
Cuts at: 3179 3921 4006 5077 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 338 257 177
146 85 84 35
BspEI T'CCGG_A
Cuts at: 2010 5523 2010
Size: 3513 2347
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2624 3215 844
Size: 1780 591 3489
Fragments arranged by size:
3489 1780 591
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2071 794
Size: 1277 4583
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 3091 3091
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2612 3190 3557 3942 3959 5411 5497 5509
Size: 640 578 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 640 578 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2271 2688 513
Size: 552 1206 417 3685
Fragments arranged by size:
3685 1206 552 417
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2319 2969 3376 4170 4190 2319
Size: 650 407 794 20 3989
Fragments arranged by size:
3989 794 650 407 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 2649 2952
Size: 54 1425 50 275 18 364 455 303
Cuts at: 2952 3081 3307 4196 5825 8
Size: 129 226 889 1629 43
Fragments arranged by size:
1629 1425 889 455 364 303 275 226
129 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2185 2400 3790 1353
Size: 832 215 1390 3423
Fragments arranged by size:
3423 1390 832 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2735 2735
Size: 5860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2325 2370 2807 2981 3923 4520
Size: 393 431 262 45 437 174 942 597
Cuts at: 4520 5307 5575 1239
Size: 787 268 1524
Fragments arranged by size:
1524 942 787 597 437 431 393 268
262 174 45
EcoO109I rG'GnC_Cy
Cuts at: 2477 2568 2707 2477
Size: 91 139 5630
Fragments arranged by size:
5630 139 91
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
EcoRV GAT'ATC
Cuts at: 2501 2501
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 5007 1678
Size: 3329 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2490 2517 2522 2560
Size: 32 135 32 913 5 27 5 38
Cuts at: 2560 2592 4501 4533 4575 4607 1373
Size: 32 1909 32 42 32 2626
Fragments arranged by size:
2626 1909 913 135 42 38 32 32
32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2464 3204 3450 669
Size: 988 281 238 288 740 246 3079
Fragments arranged by size:
3079 988 740 288 281 246 238
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3138 3185 3138
Size: 47 5813
MfeI C'AATT_G
Cuts at: 2133 3459 2133
Size: 1326 4534
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2651 2954 3083 10
Size: 54 1425 343 819 303 129 2787
Fragments arranged by size:
2787 1425 819 343 303 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 2912 2948 2981 3818 4177 4336
Size: 648 1383 363 36 33 837 359 159
Cuts at: 4336 518
Size: 2042
Fragments arranged by size:
2042 1383 837 648 363 359 159 36
33
MspA1I CmG'CkG
Cuts at: 1606 2035 2068 3016 3276 3953 5101 5346 1606
Size: 429 33 948 260 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 429 260 245 33
NcoI C'CATG_G
Cuts at: 513 1065 2688 513
Size: 552 1623 3685
Fragments arranged by size:
3685 1623 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2113 2219 3218 4763 1132
Size: 97 884 106 999 1545 2229
Fragments arranged by size:
2229 1545 999 884 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PfoI T'CCnGG_A
Cuts at: 2100 2958 2100
Size: 858 5002
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2568 2568
Size: 5860
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2476 2476
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2923 3212 830
Size: 2093 289 3478
Fragments arranged by size:
3478 2093 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2068 3016 2068
Size: 948 4912
SacI G_AGCT'C
Cuts at: 2737 2737
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2837 2919 3208 4343 5024 5215 826
Size: 219 1792 82 289 1135 681 191 1471
Fragments arranged by size:
1792 1471 1135 681 289 219 191 82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 2417 3187 1872
Size: 545 770 4545
Fragments arranged by size:
4545 770 545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2688 2715 2868 3299 513
Size: 552 1623 27 153 431 3074
Fragments arranged by size:
3074 1623 552 431 153 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3887 3904 4057 4242 4687 325
Size: 1255 2307 17 153 185 445 1498
Fragments arranged by size:
2307 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4106 5818 96
Size: 274 80 33 51 1443 2129 1712 138
Fragments arranged by size:
2129 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2947 3013 2947
Size: 66 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 2415 3185 1870
Size: 545 770 4545
Fragments arranged by size:
4545 770 545
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI