Restriction map for
pFN21AE6388
(Circular) (Six-base) MAPSORT of: pFN21AE6388.seq Check: 9908 from: 1 to: 5836
pFN21AE6388 5836 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 2343 3963 4336 1563
Size: 780 1620 373 3063
Fragments arranged by size:
3063 1620 780 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 2450 3899 5283 5551 1632
Size: 668 150 1449 1384 268 1917
Fragments arranged by size:
1917 1449 1384 668 268 150
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 2111 1166
Size: 945 4891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2837 1184
Size: 1653 4183
AlwNI CAG_nnn'CTG
Cuts at: 2636 3579 5151 2636
Size: 943 1572 3321
Fragments arranged by size:
3321 1572 943
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2665 3145 3371 3523 5632 1493
Size: 1172 480 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 480 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2323 3104 3161 1870
Size: 73 380 781 57 4545
Fragments arranged by size:
4545 781 380 73 57
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2330 3056 3157 4612 618
Size: 325 217 627 543 726 101 1455 1842
Fragments arranged by size:
1842 1455 726 627 543 325 217 101
BanII G_rGCy'C
Cuts at: 1391 2100 2238 2797 3103 3155 1391
Size: 709 138 559 306 52 4072
Fragments arranged by size:
4072 709 559 306 138 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2456 928
Size: 971 557 4308
Fragments arranged by size:
4308 971 557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2328 3079 4447
Size: 107 122 71 631 605 656 751 1368
Cuts at: 4447 136
Size: 1525
Fragments arranged by size:
1525 1368 751 656 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 2029 2844 3253 2029
Size: 815 409 4612
Fragments arranged by size:
4612 815 409
Bme1580I G_kGCm'C
Cuts at: 1790 2333 3897 5053 1790
Size: 543 1564 1156 2573
Fragments arranged by size:
2573 1564 1156 543
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2114 2479 2810 2811 4496 1239
Size: 857 18 365 331 1 1685 2579
Fragments arranged by size:
2579 1685 857 365 331 18 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 3961 4826 5124 2570
Size: 1391 865 298 3282
Fragments arranged by size:
3282 1391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2188 3017 4499 882
Size: 617 689 829 1482 2219
Fragments arranged by size:
2219 1482 829 689 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 2373 2813 5830 2373
Size: 440 3017 2379
Fragments arranged by size:
3017 2379 440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2169 4269 4667 4941 5088 5499 2169
Size: 2100 398 274 147 411 2506
Fragments arranged by size:
2506 2100 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5611
Fragments arranged by size:
5611 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2072 2956 1186
Size: 883 3 884 4066
Fragments arranged by size:
4066 884 883 3
BseYI C'CCAG_C
Cuts at: 5039 5535 5039
Size: 496 5340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2788 1163
Size: 573 1052 4211
Fragments arranged by size:
4211 1052 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2797 2850 3897 3982 5053 2100
Size: 697 53 1047 85 1071 2883
Fragments arranged by size:
2883 1071 1047 697 85 53
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2238 2333 2797 2850 3103 3155
Size: 399 310 138 95 464 53 253 52
Cuts at: 3155 3897 3982 5053 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 464 399 310 253 138
95 85 53 52
BspEI T'CCGG_A
Cuts at: 2169 5499 2169
Size: 3330 2506
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2205 4331 4505 65
Size: 1183 957 2126 174 1396
Fragments arranged by size:
2126 1396 1183 957 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4480 4667 1700
Size: 110 2670 187 2869
Fragments arranged by size:
2869 2670 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2530 2530
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2166 2374 2657 3166 3533 3918 3935 5387
Size: 194 208 283 509 367 385 17 1452
Cuts at: 5387 5473 5485 5831 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 509 385 367 346 283 208
194 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2160 2406 513
Size: 552 1095 246 3943
Fragments arranged by size:
3943 1095 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2838 3352 4146 4166 2838
Size: 514 794 20 4508
Fragments arranged by size:
4508 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2738 2773 529
Size: 35 2174 35 3592
Fragments arranged by size:
3592 2174 35 35
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2409 2561
Size: 54 1425 50 275 18 432 147 152
Cuts at: 2561 2713 3115 3283 4172 5801 8
Size: 152 402 168 889 1629 43
Fragments arranged by size:
1629 1425 889 432 402 275 168 152
152 147 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2894 3766 1353
Size: 1541 872 3423
Fragments arranged by size:
3423 1541 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2098 2795 2098
Size: 697 5139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2114 2300 2450 2479 2810 2811
Size: 393 464 18 186 150 29 331 1
Cuts at: 2811 3899 4496 5283 5551 1239
Size: 1088 597 787 268 1524
Fragments arranged by size:
1524 1088 787 597 464 393 331 268
186 150 29 18 1
EcoO109I rG'GnC_Cy
Cuts at: 2611 2611
Size: 5836
EcoRI G'AATT_C
Cuts at: 2665 3145 3523 2665
Size: 480 378 4978
Fragments arranged by size:
4978 480 378
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373
Size: 32 135 32 2905 32 42 32 2626
Fragments arranged by size:
2905 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 2014 3435 2014
Size: 1421 4415
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 2563 2715 3117 10
Size: 54 1425 343 432 299 152 402 2729
Fragments arranged by size:
2729 1425 432 402 343 299 152 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 2123 2210 2387 2528 2819 3794
Size: 648 945 12 87 177 141 291 975
Cuts at: 3794 4153 4312 518
Size: 359 159 2042
Fragments arranged by size:
2042 975 945 648 359 291 177 159
141 87 12
MspA1I CmG'CkG
Cuts at: 1606 2896 3252 3929 5077 5322 1606
Size: 1290 356 677 1148 245 2120
Fragments arranged by size:
2120 1290 1148 677 356 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2199 2416 1348
Size: 851 217 4768
Fragments arranged by size:
4768 851 217
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 2456 2456
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2611 2611
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2896 2896
Size: 5836
SacI G_AGCT'C
Cuts at: 2100 2797 2100
Size: 697 5139
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SexAI A'CCwGG_T
Cuts at: 2450 2450
Size: 5836
SfcI C'TryA_G
Cuts at: 826 1045 3184 4319 5000 5191 826
Size: 219 2139 1135 681 191 1471
Fragments arranged by size:
2139 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3079 3079
Size: 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 2325 3163 1872
Size: 453 838 4545
Fragments arranged by size:
4545 838 453
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 3104 3940 4841 5103 820
Size: 197 926 642 519 836 901 262 1553
Fragments arranged by size:
1553 926 901 836 642 519 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 2803 3760 5
Size: 47 2751 957 2081
Fragments arranged by size:
2751 2081 957 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 3275 513
Size: 552 2210 3074
Fragments arranged by size:
3074 2210 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 2145 2177 5796 5828 2064
Size: 32 49 32 3619 32 2072
Fragments arranged by size:
3619 2072 49 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2900 3034 2125
Size: 775 134 4927
Fragments arranged by size:
4927 775 134
XhoI C'TCGA_G
Cuts at: 1943 3104 1943
Size: 1161 4675
XmaI C'CCGG_G
Cuts at: 1870 2323 3161 1870
Size: 453 838 4545
Fragments arranged by size:
4545 838 453
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI