Restriction map for
pFN21AE6383
(Circular) (Six-base) MAPSORT of: pFN21AE6383.seq Check: 6611 from: 1 to: 5692
pFN21AE6383 5692 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2440 3755 5139 5407 1632
Size: 808 1315 1384 268 1917
Fragments arranged by size:
1917 1384 1315 808 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2731 1184
Size: 1547 4145
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2837 3001 3227 3379 5488 1493
Size: 1344 164 226 152 2109 1697
Fragments arranged by size:
2109 1697 1344 226 164 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2687 3013 4468 618
Size: 325 217 627 900 326 1455 1842
Fragments arranged by size:
1842 1455 900 627 326 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2872 3011 1391
Size: 700 781 139 4072
Fragments arranged by size:
4072 781 700 139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2803 3584 4794 2803
Size: 781 1210 3701
Fragments arranged by size:
3701 1210 781
BclI T'GATC_A
Cuts at: 1548 2580 2845 1548
Size: 1032 265 4395
Fragments arranged by size:
4395 1032 265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 3753 4909 1790
Size: 1963 1156 2573
Fragments arranged by size:
2573 1963 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4352 1239
Size: 3113 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2593 3817 4682 4980 2593
Size: 1224 865 298 3305
Fragments arranged by size:
3305 1224 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2638 3483 493
Size: 711 1434 845 2702
Fragments arranged by size:
2702 1434 845 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2108 1186
Size: 913 9 4770
Fragments arranged by size:
4770 913 9
BseYI C'CCAG_C
Cuts at: 2019 2034 4895 5391 2019
Size: 15 2861 496 2320
Fragments arranged by size:
2861 2320 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2701 1163
Size: 573 965 4154
Fragments arranged by size:
4154 965 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2074 3142 3903 4931 1540
Size: 275 186 73 1068 761 1028 2301
Fragments arranged by size:
2301 1068 1028 761 275 186 73
BsiHKAI G_wGCw'C
Cuts at: 2091 2872 3753 3838 4909 2091
Size: 781 881 85 1071 2874
Fragments arranged by size:
2874 1071 881 781 85
BsmI GAATG_Cn'
Cuts at: 2788 3203 3296 2788
Size: 415 93 5184
Fragments arranged by size:
5184 415 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2872 3011 3753 3838 4909 1391
Size: 399 301 781 139 742 85 1071 2174
Fragments arranged by size:
2174 1071 781 742 399 301 139 85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 2694 2902 3581 5311 2694
Size: 208 679 1730 3075
Fragments arranged by size:
3075 1730 679 208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 3047 844
Size: 1274 929 3489
Fragments arranged by size:
3489 1274 929
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2687 2783 794
Size: 1893 96 3703
Fragments arranged by size:
3703 1893 96
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2724 3022 3389 3774 3791 5243 5329 5341
Size: 752 298 367 385 17 1452 86 12
Cuts at: 5341 5687 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 752 385 367 346 298 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2805 513
Size: 552 939 801 3400
Fragments arranged by size:
3400 939 801 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 3208 4002 4022 2091
Size: 1117 794 20 3761
Fragments arranged by size:
3761 1117 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DraIII CAC_nnn'GTG
Cuts at: 2678 2678
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2071 2497
Size: 54 1425 50 275 18 214 27 426
Cuts at: 2497 3139 4028 5657 8
Size: 642 889 1629 43
Fragments arranged by size:
1629 1425 889 642 426 275 214 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2071 3139 1537
Size: 275 259 1068 4090
Fragments arranged by size:
4090 1068 275 259
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2089 2870 2089
Size: 781 4911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2440 3755 4352 5139 5407 1239
Size: 393 808 1315 597 787 268 1524
Fragments arranged by size:
1524 1315 808 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2756 2756
Size: 5692
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4333 4365 4407 4439 1373
Size: 32 135 32 2761 32 42 32 2626
Fragments arranged by size:
2761 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 4806 4990 1305
Size: 1098 2403 184 2007
Fragments arranged by size:
2403 2007 1098 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2499 10
Size: 54 1425 343 667 3203
Fragments arranged by size:
3203 1425 667 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3650 4009 4168 518
Size: 648 2484 359 159 2042
Fragments arranged by size:
2484 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 2351 2503 2572 2711 3108 3785 4933
Size: 473 272 152 69 139 397 677 1148
Cuts at: 4933 5178 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 473 397 272 245 152
139 69
NcoI C'CATG_G
Cuts at: 513 1065 2004 2805 513
Size: 552 939 801 3400
Fragments arranged by size:
3400 939 801 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 2071 3139 2071
Size: 1068 4624
NspI r_CATG'y
Cuts at: 1132 1229 3050 4595 1132
Size: 97 1821 1545 2229
Fragments arranged by size:
2229 1821 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2204 1348
Size: 856 4836
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2675 1108
Size: 902 665 4125
Fragments arranged by size:
4125 902 665
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 2571 3044 830
Size: 1741 473 3478
Fragments arranged by size:
3478 1741 473
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2079 2351 2711 2079
Size: 272 360 5060
Fragments arranged by size:
5060 360 272
SacI G_AGCT'C
Cuts at: 2091 2872 2091
Size: 781 4911
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2567 3040 4175 4856 5047 826
Size: 219 1522 473 1135 681 191 1471
Fragments arranged by size:
1522 1471 1135 681 473 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2608 3796 4697 4959 820
Size: 197 926 665 1188 901 262 1553
Fragments arranged by size:
1553 1188 926 901 665 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2805 3131 513
Size: 552 939 801 326 3074
Fragments arranged by size:
3074 939 801 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 3719 3736 3889 4074 4519 325
Size: 1255 608 1531 17 153 185 445 1498
Fragments arranged by size:
1531 1498 1255 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2200 2232 2584 2616 5652 5684 2200
Size: 32 352 32 3036 32 2208
Fragments arranged by size:
3036 2208 352 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI