Restriction map for
pFN21AE6382
(Circular) (Six-base) MAPSORT of: pFN21AE6382.seq Check: 7344 from: 1 to: 5686
pFN21AE6382 5686 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2710 2838 3029 1937
Size: 773 128 191 4594
Fragments arranged by size:
4594 773 191 128
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 2678 3749 5133 5401 1632
Size: 692 354 1071 1384 268 1917
Fragments arranged by size:
1917 1384 1071 692 354 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 2256 4585 5562 1203
Size: 1053 2329 977 1327
Fragments arranged by size:
2329 1327 1053 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 2402 1166
Size: 1236 4450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 2251 2302 3429 5001 2251
Size: 51 1127 1572 2936
Fragments arranged by size:
2936 1572 1127 51
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2595 2995 3221 3373 5482 1493
Size: 1102 400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1102 400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BamHI G'GATC_C
Cuts at: 2917 3016 3383 2917
Size: 99 367 5220
Fragments arranged by size:
5220 367 99
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 4462 618
Size: 325 217 627 1220 1455 1842
Fragments arranged by size:
1842 1455 1220 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 3005 1391
Size: 673 941 4072
Fragments arranged by size:
4072 941 673
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3877 3911 1903
Size: 34 1940 34 3678
Fragments arranged by size:
3678 1940 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 2484 1548
Size: 936 4750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2900 5681 2900
Size: 2781 2905
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 3747 4903 1790
Size: 1957 1156 2573
Fragments arranged by size:
2573 1957 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2078 2515 4346 1239
Size: 824 15 437 1831 2579
Fragments arranged by size:
2579 1831 824 437 15
Bpu10I CC'TnA_GC
Cuts at: 2457 2457
Size: 5686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 4349 882
Size: 617 531 2319 2219
Fragments arranged by size:
2319 2219 617 531
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 2905 5680 2905
Size: 2775 2911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5686
BseYI C'CCAG_C
Cuts at: 2578 4889 5385 2578
Size: 2311 496 2879
Fragments arranged by size:
2879 2311 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 1163
Size: 573 333 4780
Fragments arranged by size:
4780 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3747 3832 4903 2064
Size: 1683 85 1071 2847
Fragments arranged by size:
2847 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 2763 3197 3290 2763
Size: 434 93 5159
Fragments arranged by size:
5159 434 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 3005 3747 3832 4903 1391
Size: 399 274 941 742 85 1071 2174
Fragments arranged by size:
2174 1071 941 742 399 274 85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2382 3041 844
Size: 1538 659 3489
Fragments arranged by size:
3489 1538 659
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2969 4181 4355 65
Size: 1183 1721 1212 174 1396
Fragments arranged by size:
1721 1396 1212 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2238 4330 4517 1700
Size: 110 211 217 2092 187 2869
Fragments arranged by size:
2869 2092 217 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 2634 96
Size: 2538 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2971 794
Size: 2177 3509
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 2452 2733 2900 2917 3016 3383 3768 3785
Size: 480 281 167 17 99 367 385 17
Cuts at: 3785 5237 5323 5335 5681 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 480 385 367 346 281 167
99 86 17 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2615 513
Size: 552 1550 3584
Fragments arranged by size:
3584 1550 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2795 3202 3996 4016 2795
Size: 407 794 20 4465
Fragments arranged by size:
4465 794 407 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5651
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 3133 4022
Size: 54 1425 50 275 18 498 805 889
Cuts at: 4022 5651 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 805 498 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2891 3616 1353
Size: 1538 725 3423
Fragments arranged by size:
3423 1538 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2201 4273 4647 4793 387
Size: 1064 138 612 2072 374 146 1280
Fragments arranged by size:
2072 1280 1064 612 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 5686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2078 2324 2515 2678 3749 4346
Size: 393 431 15 246 191 163 1071 597
Cuts at: 4346 5133 5401 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1071 787 597 431 393 268 246
191 163 15
EcoO109I rG'GnC_Cy
Cuts at: 2323 2323
Size: 5686
EcoRI G'AATT_C
Cuts at: 2595 2995 3373 2595
Size: 400 378 4908
Fragments arranged by size:
4908 400 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 5686
FspI TGC'GCA
Cuts at: 2147 4192 2147
Size: 2045 3641
HaeII r_GCGC'y
Cuts at: 1678 4833 1678
Size: 3155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2437 2469 4327 4359 4401
Size: 32 135 32 865 32 1858 32 42
Cuts at: 4401 4433 1373
Size: 32 2626
Fragments arranged by size:
2626 1858 865 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2266 748
Size: 1518 4168
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 2377 3285 2377
Size: 908 4778
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 10
Size: 54 1425 343 498 3366
Fragments arranged by size:
3366 1425 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2811 2855 3644 4003 4162 518
Size: 648 1236 409 44 789 359 159 2042
Fragments arranged by size:
2042 1236 789 648 409 359 159 44
MspA1I CmG'CkG
Cuts at: 1606 3102 3779 4927 5172 1606
Size: 1496 677 1148 245 2120
Fragments arranged by size:
2120 1496 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2615 513
Size: 552 1550 3584
Fragments arranged by size:
3584 1550 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 2260 2854 2965 3044 4589 1132
Size: 97 1031 594 111 79 1545 2229
Fragments arranged by size:
2229 1545 1031 594 111 97 79
PasI CC'CwG_GG
Cuts at: 1348 2318 1348
Size: 970 4716
PciI A'CATG_T
Cuts at: 2256 4585 2256
Size: 2329 3357
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PfoI T'CCnGG_A
Cuts at: 2097 2097
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2568 3038 830
Size: 1738 470 3478
Fragments arranged by size:
3478 1738 470
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 2568 3038 2568
Size: 470 5216
ScaI AGT'ACT
Cuts at: 2228 2928 3934 5646 2228
Size: 700 1006 1712 2268
Fragments arranged by size:
2268 1712 1006 700
SfcI C'TryA_G
Cuts at: 826 1045 2564 2845 2922 3034 4169 4850 5041
Size: 219 1519 281 77 112 1135 681 191
Cuts at: 5041 826
Size: 1471
Fragments arranged by size:
1519 1471 1135 681 281 219 191 112
77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 2854 3044 1132
Size: 1722 190 3774
Fragments arranged by size:
3774 1722 190
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 2207 2562 1517
Size: 690 355 4641
Fragments arranged by size:
4641 690 355
StyI C'CwwG_G
Cuts at: 513 1065 2615 3125 513
Size: 552 1550 510 3074
Fragments arranged by size:
3074 1550 552 510
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 3730 3883 4068 4513 325
Size: 1255 2133 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2634 2829
Size: 274 80 33 51 1443 249 408 195
Cuts at: 2829 2926 3932 5644 96
Size: 97 1006 1712 138
Fragments arranged by size:
1712 1443 1006 408 274 249 195 138
97 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI