Restriction map for
pFN21AE6375
(Circular) (Six-base) MAPSORT of: pFN21AE6375.seq Check: 6733 from: 1 to: 5905
pFN21AE6375 5905 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2622 3045 3715 278
Size: 53 83 186 936 1086 423 670 2468
Fragments arranged by size:
2468 1086 936 670 423 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2611 2920 3077 3968 5352 5620 1632
Size: 434 545 309 157 891 1384 268 1917
Fragments arranged by size:
1917 1384 891 545 434 309 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 3020 4634 3020
Size: 1614 4291
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 3113 3648 5220 3113
Size: 535 1572 3798
Fragments arranged by size:
3798 1572 535
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2335 3017 3230 1870
Size: 73 392 682 213 4545
Fragments arranged by size:
4545 682 392 213 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 2551 3139 3226 4681
Size: 325 217 627 449 315 588 87 1455
Cuts at: 4681 618
Size: 1842
Fragments arranged by size:
1842 1455 627 588 449 325 315 217
87
BanII G_rGCy'C
Cuts at: 1391 2220 3224 1391
Size: 829 1004 4072
Fragments arranged by size:
4072 1004 829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2614 3016 3028 3040 928
Size: 971 715 402 12 12 3793
Fragments arranged by size:
3793 971 715 402 12 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3145 3179 4096 4130 1903
Size: 34 1208 34 917 34 3678
Fragments arranged by size:
3678 1208 917 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3797 5007 2334
Size: 1463 1210 3232
Fragments arranged by size:
3232 1463 1210
BclI T'GATC_A
Cuts at: 1548 2169 2442 2655 2781 1548
Size: 621 273 213 126 4672
Fragments arranged by size:
4672 621 273 213 126
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2556 3142 3966 5122 1790
Size: 766 586 824 1156 2573
Fragments arranged by size:
2573 1156 824 766 586
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3145 3636 4594
Size: 416 53 83 399 24 1728 491 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1728 958 491 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2265 2914 2942 4565 1239
Size: 887 139 649 28 1623 2579
Fragments arranged by size:
2579 1623 887 649 139 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 4030 4895 5193 2234
Size: 1796 865 298 2946
Fragments arranged by size:
2946 1796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2991 3076 4568 882
Size: 617 1492 85 1492 2219
Fragments arranged by size:
2219 1492 1492 617 85
BsaAI yAC'GTr
Cuts at: 493 1204 2787 3696 493
Size: 711 1583 909 2702
Fragments arranged by size:
2702 1583 909 711
BsaBI GATnn'nnATC
Cuts at: 2660 5899 2660
Size: 3239 2666
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2619 3042 3712 4094
Size: 53 83 186 936 1086 423 670 382
Cuts at: 4094 4729 275
Size: 635 1451
Fragments arranged by size:
1451 1086 936 670 635 423 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2074 2104 1178
Size: 30 165 30 671 30 4979
Fragments arranged by size:
4979 671 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 2534 2567 1186
Size: 1062 286 33 4524
Fragments arranged by size:
4524 1062 286 33
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 2687 1163
Size: 573 882 69 4381
Fragments arranged by size:
4381 882 573 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 2733 2913 3966 4051 5122 2220
Size: 513 180 1053 85 1071 3003
Fragments arranged by size:
3003 1071 1053 513 180 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
BsmBI CGTCTCn'nnnn_
Cuts at: 3128 3128
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2556 2733 2913 3142 3224 3966
Size: 399 430 336 177 180 229 82 742
Cuts at: 3966 4051 5122 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 430 399 336 229 180
177 85 82
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 2445 3794 5524 2445
Size: 1349 1730 2826
Fragments arranged by size:
2826 1730 1349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2330 4549 4736 1700
Size: 110 520 2219 187 2869
Fragments arranged by size:
2869 2219 520 187 110
BsrGI T'GTAC_A
Cuts at: 96 3072 96
Size: 2976 2929
BssHII G'CGCG_C
Cuts at: 1925 3101 1925
Size: 1176 4729
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 2393 2764 2393
Size: 371 5534
BstYI r'GATC_y
Cuts at: 1972 2400 3235 3602 3987 4004 5456 5542 5554
Size: 428 835 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 835 428 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2436 2436
Size: 5905
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 2675 2778 3421 4215 4235 2675
Size: 103 643 794 20 4345
Fragments arranged by size:
4345 794 643 103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2144 2692 3352
Size: 54 1425 50 275 18 314 548 660
Cuts at: 3352 4241 5870 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 660 548 314 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2747 2807 3835 1353
Size: 1394 60 1028 3423
Fragments arranged by size:
3423 1394 1028 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2473 4492 4866 5012 387
Size: 1064 138 884 2019 374 146 1280
Fragments arranged by size:
2019 1280 1064 884 374 146 138
EcoICRI GAG'CTC
Cuts at: 2218 2218
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2126 2265 2611 2914 2920 2942
Size: 393 434 60 139 346 303 6 22
Cuts at: 2942 3077 3968 4565 5352 5620 1239
Size: 135 891 597 787 268 1524
Fragments arranged by size:
1524 891 787 597 434 393 346 303
268 139 135 60 22 6
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2370 5052 1678
Size: 692 2682 2531
Fragments arranged by size:
2682 2531 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3006 3038 4546 4578 4620
Size: 32 135 32 1434 32 1508 32 42
Cuts at: 4620 4652 1373
Size: 32 2626
Fragments arranged by size:
2626 1508 1434 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3145 748
Size: 2397 3508
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2266 5019 5203 1305
Size: 961 2753 184 2007
Fragments arranged by size:
2753 2007 961 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2694 10
Size: 54 1425 343 862 3221
Fragments arranged by size:
3221 1425 862 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 2762 3863 4222 4381 518
Size: 648 1497 99 1101 359 159 2042
Fragments arranged by size:
2042 1497 1101 648 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 3321 3998 5146 5391 1606
Size: 1715 677 1148 245 2120
Fragments arranged by size:
2120 1715 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2242 2913 2242
Size: 671 5234
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PmlI CAC'GTG
Cuts at: 2787 2787
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2102 2134 3974 4006 5487 5519 2102
Size: 32 1840 32 1481 32 2488
Fragments arranged by size:
2488 1840 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2126 1613
Size: 513 5392
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 5873
PstI C_TGCA'G
Cuts at: 830 2773 2796 2949 3257 830
Size: 1943 23 153 308 3478
Fragments arranged by size:
3478 1943 308 153 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
SacI G_AGCT'C
Cuts at: 2220 2220
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SapI GCTCTTCn'nnn_
Cuts at: 2747 2747
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SexAI A'CCwGG_T
Cuts at: 2385 2385
Size: 5905
SfcI C'TryA_G
Cuts at: 826 1045 2521 2769 2792 2945 3253 4388 5069
Size: 219 1476 248 23 153 308 1135 681
Cuts at: 5069 5260 826
Size: 191 1471
Fragments arranged by size:
1476 1471 1135 681 308 248 219 191
153 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 4009 4910 5172 820
Size: 197 926 270 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 270 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 2203 3829 5
Size: 47 2151 1626 2081
Fragments arranged by size:
2151 2081 1626 47
StuI AGG'CCT
Cuts at: 1517 2858 3161 1517
Size: 1341 303 4261
Fragments arranged by size:
4261 1341 303
StyI C'CwwG_G
Cuts at: 513 1065 3344 513
Size: 552 2279 3074
Fragments arranged by size:
3074 2279 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2539 3932 3949 4102 4287 4732 325
Size: 1255 959 1393 17 153 185 445 1498
Fragments arranged by size:
1498 1393 1255 959 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3072 4151 5863
Size: 274 80 33 51 1443 1095 1079 1712
Cuts at: 5863 96
Size: 138
Fragments arranged by size:
1712 1443 1095 1079 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2074 2106 5865 5897 2074
Size: 32 3759 32 2082
Fragments arranged by size:
3759 2082 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3044 1316
Size: 1728 4177
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2722
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2620 3043 3713 276
Size: 53 83 186 936 1086 423 670 2468
Fragments arranged by size:
2468 1086 936 670 423 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PstI PvuI SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PvuII
RsrII SacII SanDI SfiI SfoI SrfI SwaI