Restriction map for
pFN21AE6374
(Circular) (Six-base) MAPSORT of: pFN21AE6374.seq Check: 1160 from: 1 to: 6022
pFN21AE6374 6022 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2156 2249 2564 2156
Size: 93 315 5614
Fragments arranged by size:
5614 315 93
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3832 278
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2641 3365 1937
Size: 704 724 4594
Fragments arranged by size:
4594 724 704
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3041 3177 4085 5469 5737 1632
Size: 1409 136 908 1384 268 1917
Fragments arranged by size:
1917 1409 1384 908 268 136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 2429 4921 5898 1203
Size: 1226 2492 977 1327
Fragments arranged by size:
2492 1327 1226 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 2368 3194 3283 1166
Size: 1202 826 89 3905
Fragments arranged by size:
3905 1202 826 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6022
AlwNI CAG_nnn'CTG
Cuts at: 3765 5337 3765
Size: 1572 4450
ApaI G_GGCC'C
Cuts at: 2126 2126
Size: 6022
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 2777 3331 3557 3709 5818 1493
Size: 1284 554 226 152 2109 1697
Fragments arranged by size:
2109 1697 1284 554 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2848 3347 1870
Size: 73 905 499 4545
Fragments arranged by size:
4545 905 499 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2604 2637 2604
Size: 33 5989
BamHI G'GATC_C
Cuts at: 3073 3352 3719 3073
Size: 279 367 5376
Fragments arranged by size:
5376 367 279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3343 4798 618
Size: 325 217 627 1556 1455 1842
Fragments arranged by size:
1842 1556 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2126 2275 2855 3341 1391
Size: 735 149 580 486 4072
Fragments arranged by size:
4072 735 580 486 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2586 2620 4213 4247 1903
Size: 34 649 34 1593 34 3678
Fragments arranged by size:
3678 1593 649 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3914 5124 3914
Size: 1210 4812
BclI T'GATC_A
Cuts at: 1548 2671 1548
Size: 1123 4899
BfrBI ATG'CAT
Cuts at: 3213 3213
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4633 136
Size: 107 122 71 631 605 2961 1525
Fragments arranged by size:
2961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 2274 2284 2932 3439 2274
Size: 10 648 507 4857
Fragments arranged by size:
4857 648 507 10
Bme1580I G_kGCm'C
Cuts at: 1790 2126 4083 5239 1790
Size: 336 1957 1156 2573
Fragments arranged by size:
2573 1957 1156 336
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6022
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3753 4711 442
Size: 416 53 83 399 24 2336 958 1753
Fragments arranged by size:
2336 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2684 4682 1239
Size: 1445 1998 2579
Fragments arranged by size:
2579 1998 1445
Bpu10I CC'TnA_GC
Cuts at: 2186 2186
Size: 6022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 4147 5012 5310 2351
Size: 1796 865 298 3063
Fragments arranged by size:
3063 1796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3294 4685 882
Size: 617 1795 1391 2219
Fragments arranged by size:
2219 1795 1391 617
BsaAI yAC'GTr
Cuts at: 493 1204 3813 493
Size: 711 2609 2702
Fragments arranged by size:
2702 2609 711
BsaBI GATnn'nnATC
Cuts at: 3233 6016 3233
Size: 2783 3239
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3829 4211 4846 275
Size: 53 83 186 936 2296 382 635 1451
Fragments arranged by size:
2296 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3292 4455 4853 5127 5274 5685 3292
Size: 1163 398 274 147 411 3629
Fragments arranged by size:
3629 1163 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2837 2867 2980 3010 1178
Size: 30 165 30 1434 30 113 30 4190
Fragments arranged by size:
4190 1434 165 113 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2105 2961 3001 3109 3112 3182 1186
Size: 847 72 856 40 108 3 70 4026
Fragments arranged by size:
4026 856 847 108 72 70 40 3
BseYI C'CCAG_C
Cuts at: 2500 2586 5225 5721 2500
Size: 86 2639 496 2801
Fragments arranged by size:
2801 2639 496 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5449
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3472 4233 5261 1540
Size: 275 186 1471 761 1028 2301
Fragments arranged by size:
2301 1471 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2275 3119 4083 4168 5239 2275
Size: 844 964 85 1071 3058
Fragments arranged by size:
3058 1071 964 844 85
BsmI GAATG_Cn'
Cuts at: 2015 2141 3533 3626 2015
Size: 126 1392 93 4411
Fragments arranged by size:
4411 1392 126 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2906 2906
Size: 6022
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2275 2855 3119 3341 4083 4168
Size: 399 336 149 580 264 222 742 85
Cuts at: 4168 5239 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 580 399 336 264 222
149 85
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 3911 5641 3911
Size: 1730 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2156 2249 2564 3377 844
Size: 1312 93 315 813 3489
Fragments arranged by size:
3489 1312 813 315 93
BsrBI CCG'CTC
Cuts at: 1394 1823 3909 1394
Size: 429 2086 3507
Fragments arranged by size:
3507 2086 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2285 4517 4691 65
Size: 1183 1037 2232 174 1396
Fragments arranged by size:
2232 1396 1183 1037 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2559 4666 4853 1700
Size: 110 749 2107 187 2869
Fragments arranged by size:
2869 2107 749 187 110
BsrGI T'GTAC_A
Cuts at: 96 3216 96
Size: 3120 2902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 3124 4082 5094 3124
Size: 958 1012 4052
Fragments arranged by size:
4052 1012 958
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 4826 4826
Size: 6022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstXI CCAn_nnnn'nTGG
Cuts at: 2483 3090 2483
Size: 607 5415
BstYI r'GATC_y
Cuts at: 1972 3073 3352 3719 4104 4121 5573 5659 5671
Size: 1101 279 367 385 17 1452 86 12
Cuts at: 5671 6017 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1101 385 367 346 279 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2412 2412
Size: 6022
BtgI C'CryG_G
Cuts at: 513 1065 2004 2126 2308 3104 513
Size: 552 939 122 182 796 3431
Fragments arranged by size:
3431 939 796 552 182 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3220 487
Size: 683 56 1994 3289
Fragments arranged by size:
3289 1994 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 3538 4332 4352 2036
Size: 1502 794 20 3706
Fragments arranged by size:
3706 1502 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2867 5029 809
Size: 507 1551 2162 1802
Fragments arranged by size:
2162 1802 1551 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2311 3469 4358
Size: 54 1425 50 275 18 481 1158 889
Cuts at: 4358 5987 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1158 889 481 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 1537
Size: 275 1657 4090
Fragments arranged by size:
4090 1657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3195 3952 1353
Size: 1842 757 3423
Fragments arranged by size:
3423 1842 757
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4609 4983 5129 387
Size: 1064 138 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2273 2273
Size: 6022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2684 3041 3177 4085 4682 5469 5737
Size: 393 1052 357 136 908 597 787 268
Cuts at: 5737 1239
Size: 1524
Fragments arranged by size:
1524 1052 908 787 597 393 357 268
136
EcoO109I rG'GnC_Cy
Cuts at: 2122 2160 2666 2122
Size: 38 506 5478
Fragments arranged by size:
5478 506 38
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 2182 5169 1678
Size: 504 2987 2531
Fragments arranged by size:
2987 2531 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4663 4695 4737 4769 1373
Size: 32 135 32 3091 32 42 32 2626
Fragments arranged by size:
3091 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3366 3612 669
Size: 988 281 1428 246 3079
Fragments arranged by size:
3079 1428 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3053 748
Size: 2305 3717
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 2429 5898 2429
Size: 3469 2553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5136 5320 1305
Size: 3831 184 2007
Fragments arranged by size:
3831 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2313 10
Size: 54 1425 343 481 3719
Fragments arranged by size:
3719 1425 481 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2295 2368 3194 3283 3980 4339 4498
Size: 648 1129 73 826 89 697 359 159
Cuts at: 4498 518
Size: 2042
Fragments arranged by size:
2042 1129 826 697 648 359 159 89
73
MspA1I CmG'CkG
Cuts at: 1606 2500 2858 2959 3438 4115 5263 5508 1606
Size: 894 358 101 479 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 479 358 245 101
NaeI GCC'GGC
Cuts at: 2561 2561
Size: 6022
NcoI C'CATG_G
Cuts at: 513 1065 2004 2126 2308 3104 513
Size: 552 939 122 182 796 3431
Fragments arranged by size:
3431 939 796 552 182 122
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NgoMIV G'CCGG_C
Cuts at: 2559 2559
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NsiI A_TGCA'T
Cuts at: 3215 3215
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 2232 3188 3217 3380 4925 1132
Size: 97 1003 956 29 163 1545 2229
Fragments arranged by size:
2229 1545 1003 956 163 97 29
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6022
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3105 1108
Size: 1997 4025
PfoI T'CCnGG_A
Cuts at: 2072 2072
Size: 6022
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2666 2666
Size: 6022
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 3592 3592
Size: 6022
PspOMI G'GGCC_C
Cuts at: 2122 2122
Size: 6022
PstI C_TGCA'G
Cuts at: 830 3374 830
Size: 2544 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
PvuII CAG'CTG
Cuts at: 2500 2959 2500
Size: 459 5563
SacI G_AGCT'C
Cuts at: 2275 2275
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SfcI C'TryA_G
Cuts at: 826 1045 3370 4505 5186 5377 826
Size: 219 2325 1135 681 191 1471
Fragments arranged by size:
2325 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 3349 1872
Size: 1477 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 4126 5027 5289 820
Size: 197 926 423 1760 901 262 1553
Fragments arranged by size:
1760 1553 926 901 423 262 197
SnaBI TAC'GTA
Cuts at: 493 3813 493
Size: 3320 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6022
StyI C'CwwG_G
Cuts at: 513 1065 2004 2126 2308 3104 3461 513
Size: 552 939 122 182 796 357 3074
Fragments arranged by size:
3074 939 796 552 357 182 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4049 4066 4219 4404 4849 325
Size: 1255 2469 17 153 185 445 1498
Fragments arranged by size:
2469 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2864 3216 4268
Size: 274 80 33 51 1443 887 352 1052
Cuts at: 4268 5980 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1052 887 352 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2698 2730 2980 3012 5982 6014 2698
Size: 32 250 32 2970 32 2706
Fragments arranged by size:
2970 2706 250 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 3347 1870
Size: 1477 4545
XmnI GAAnn'nnTTC
Cuts at: 2328 4151 2328
Size: 1823 4199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3830 276
Size: 53 83 186 936 2296 2468
Fragments arranged by size:
2468 2296 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI