Restriction map for
pFN21AE6373
(Circular) (Six-base) MAPSORT of: pFN21AE6373.seq Check: 1158 from: 1 to: 5716
pFN21AE6373 5716 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3779 5163 5431 1632
Size: 2147 1384 268 1917
Fragments arranged by size:
2147 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 2322 1166
Size: 1156 4560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2271 2378 2696 3025 3251 3403 5512 1493
Size: 778 107 318 329 226 152 2109 1697
Fragments arranged by size:
2109 1697 778 329 318 226 152 107
AseI AT'TA_AT
Cuts at: 160 1032 2864 4270 160
Size: 872 1832 1406 1606
Fragments arranged by size:
1832 1606 1406 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 3041 1870
Size: 73 528 570 4545
Fragments arranged by size:
4545 570 528 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3037 4492 618
Size: 325 217 627 1250 1455 1842
Fragments arranged by size:
1842 1455 1250 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 3035 1391
Size: 661 983 4072
Fragments arranged by size:
4072 983 661
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4745
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2851 3608 4818 2851
Size: 757 1210 3749
Fragments arranged by size:
3749 1210 757
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4327 136
Size: 107 122 71 631 605 337 2318 1525
Fragments arranged by size:
2318 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 3777 4933 1790
Size: 1987 1156 2573
Fragments arranged by size:
2573 1987 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4405 442
Size: 416 53 83 399 24 2030 958 1753
Fragments arranged by size:
2030 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2694 4376 1239
Size: 1455 1682 2579
Fragments arranged by size:
2579 1682 1455
Bpu10I CC'TnA_GC
Cuts at: 2372 2372
Size: 5716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2953 3841 4706 5004 2953
Size: 888 865 298 3665
Fragments arranged by size:
3665 888 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2670 4379 882
Size: 617 1171 1709 2219
Fragments arranged by size:
2219 1709 1171 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 2864 5710 2864
Size: 2846 2870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 3905 4540 275
Size: 53 83 186 936 1990 382 635 1451
Fragments arranged by size:
1990 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 1186
Size: 1105 4611
BseYI C'CCAG_C
Cuts at: 2489 4919 5415 2489
Size: 2430 496 2790
Fragments arranged by size:
2790 2430 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2806 1163
Size: 573 1070 4073
Fragments arranged by size:
4073 1070 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 3166 3927 4955 1540
Size: 275 186 12 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2373 3777 3862 4933 2373
Size: 1404 85 1071 3156
Fragments arranged by size:
3156 1404 1071 85
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2373 3035 3777 3862 4933 1391
Size: 399 262 321 662 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 662 399 321 262 85
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 2637 3605 5335 2637
Size: 968 1730 3018
Fragments arranged by size:
3018 1730 968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2120 2688 3071 844
Size: 1276 568 383 3489
Fragments arranged by size:
3489 1276 568 383
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 4360 4547 1700
Size: 110 202 2348 187 2869
Fragments arranged by size:
2869 2348 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2324 2324
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711
Size: 1074 367 385 17 1452 86 12 346
Cuts at: 5711 1972
Size: 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2775 513
Size: 552 1710 3454
Fragments arranged by size:
3454 1710 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2823 3232 4026 4046 2823
Size: 409 794 20 4493
Fragments arranged by size:
4493 794 409 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2017 3163
Size: 54 1425 50 275 18 180 7 1146
Cuts at: 3163 4052 5681 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1146 889 275 180 54 50
43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2010 3163 1537
Size: 275 198 1153 4090
Fragments arranged by size:
4090 1153 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 3005 3646 1353
Size: 752 900 641 3423
Fragments arranged by size:
3423 900 752 641
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2694 3779 4376 5163 5431 1239
Size: 393 1062 1085 597 787 268 1524
Fragments arranged by size:
1524 1085 1062 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 2684
Size: 5716
EcoO109I rG'GnC_Cy
Cuts at: 2109 2109
Size: 5716
EcoRI G'AATT_C
Cuts at: 2696 3025 3403 2696
Size: 329 378 5009
Fragments arranged by size:
5009 378 329
EcoRV GAT'ATC
Cuts at: 2545 2545
Size: 5716
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2040 2099 2147 4863 1678
Size: 362 59 48 2716 2531
Fragments arranged by size:
2716 2531 362 59 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4357 4389 4431 4463 1373
Size: 32 135 32 2785 32 42 32 2626
Fragments arranged by size:
2785 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 3060 3306 669
Size: 988 281 199 923 246 3079
Fragments arranged by size:
3079 988 923 281 246 199
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2290 2803 4830 5014 1305
Size: 985 513 2027 184 2007
Fragments arranged by size:
2027 2007 985 513 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2322 3674 4033 4192 518
Size: 648 1044 112 1352 359 159 2042
Fragments arranged by size:
2042 1352 1044 648 359 159 112
MspA1I CmG'CkG
Cuts at: 1606 2489 3132 3809 4957 5202 1606
Size: 883 643 677 1148 245 2120
Fragments arranged by size:
2120 1148 883 677 643 245
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5716
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2109 2109
Size: 5716
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2489 2489
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SapI GCTCTTCn'nnn_
Cuts at: 2105 3005 2105
Size: 900 4816
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 2616 3964 5676 2616
Size: 1348 1712 2656
Fragments arranged by size:
2656 1712 1348
SfcI C'TryA_G
Cuts at: 826 1045 2729 3064 4199 4880 5071 826
Size: 219 1684 335 1135 681 191 1471
Fragments arranged by size:
1684 1471 1135 681 335 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2932 3820 4721 4983 820
Size: 197 926 989 888 901 262 1553
Fragments arranged by size:
1553 989 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 2914 3640 5
Size: 47 2862 726 2081
Fragments arranged by size:
2862 2081 726 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2182 3155 513
Size: 552 1117 973 3074
Fragments arranged by size:
3074 1117 973 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2972 3743 3760 3913 4098 4543 325
Size: 1255 1392 771 17 153 185 445 1498
Fragments arranged by size:
1498 1392 1255 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2614 2769 3962
Size: 274 80 33 51 1443 637 155 1193
Cuts at: 3962 5674 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1193 637 274 155 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 5676 5708 2163
Size: 32 3481 32 2171
Fragments arranged by size:
3481 2171 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2581 2581
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI