Restriction map for
pFN21AE6372
(Circular) (Six-base) MAPSORT of: pFN21AE6372.seq Check: 7559 from: 1 to: 5776
pFN21AE6372 5776 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 2508 3903 4276 1563
Size: 945 1395 373 3063
Fragments arranged by size:
3063 1395 945 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3017 3839 5223 5491 1632
Size: 1385 822 1384 268 1917
Fragments arranged by size:
1917 1385 1384 822 268
AfeI AGC'GCT
Cuts at: 2675 2675
Size: 5776
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 2418 4505 2418
Size: 2087 3689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2981 3519 5091 2981
Size: 538 1572 3666
Fragments arranged by size:
3666 1572 538
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2611 3101 1870
Size: 73 190 478 490 4545
Fragments arranged by size:
4545 490 478 190 73
BamHI G'GATC_C
Cuts at: 2287 3106 3473 2287
Size: 819 367 4590
Fragments arranged by size:
4590 819 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 3097 4552 618
Size: 325 217 627 359 951 1455 1842
Fragments arranged by size:
1842 1455 951 627 359 325 217
BanII G_rGCy'C
Cuts at: 1391 2035 3095 1391
Size: 644 1060 4072
Fragments arranged by size:
4072 1060 644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2573 928
Size: 971 674 4131
Fragments arranged by size:
4131 971 674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2714 3668 4878 2714
Size: 954 1210 3612
Fragments arranged by size:
3612 1210 954
BclI T'GATC_A
Cuts at: 1548 2382 2469 1548
Size: 834 87 4855
Fragments arranged by size:
4855 834 87
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2372 2553 4387
Size: 107 122 71 631 605 700 181 1834
Cuts at: 4387 136
Size: 1525
Fragments arranged by size:
1834 1525 700 631 605 181 122 107
71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2151 3837 4993 1790
Size: 361 1686 1156 2573
Fragments arranged by size:
2573 1686 1156 361
BmgBI CAC'GTC
Cuts at: 1318 2959 1318
Size: 1641 4135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2481 2546 2723 4436 1239
Size: 1242 65 177 1713 2579
Fragments arranged by size:
2579 1713 1242 177 65
Bpu10I CC'TnA_GC
Cuts at: 2781 2781
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3009 3583 3965 4600
Size: 53 83 186 936 1476 574 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1476 1451 936 635 574 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2019 2074 2760 4209 4607 4881 5028 5439 2019
Size: 55 686 1449 398 274 147 411 2356
Fragments arranged by size:
2356 1449 686 411 398 274 147 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2781 2811 1178
Size: 30 165 30 1378 30 4143
Fragments arranged by size:
4143 1378 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2174 2177 2189 2192 2204 2213 2225
Size: 940 48 3 12 3 12 9 12
Cuts at: 2225 1186
Size: 4737
Fragments arranged by size:
4737 940 48 12 12 12 9 3
3
BseYI C'CCAG_C
Cuts at: 4979 5475 4979
Size: 496 5280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3005 1163
Size: 573 1269 3934
Fragments arranged by size:
3934 1269 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3837 3922 4993 3837
Size: 85 1071 4620
Fragments arranged by size:
4620 1071 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2151 3095 3837 3922 4993 1391
Size: 399 245 116 944 742 85 1071 2174
Fragments arranged by size:
2174 1071 944 742 399 245 116 85
BspEI T'CCGG_A
Cuts at: 2074 5439 2074
Size: 3365 2411
BspHI T'CATG_A
Cuts at: 2268 3665 5395 2268
Size: 1397 1730 2649
Fragments arranged by size:
2649 1730 1397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 844
Size: 2287 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2446 3663 1394
Size: 429 623 1217 3507
Fragments arranged by size:
3507 1217 623 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2248 2644 2949 4271 4445 65
Size: 1183 1000 396 305 1322 174 1396
Fragments arranged by size:
1396 1322 1183 1000 396 305 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 3475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2260 794
Size: 1466 4310
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2287 2791 3106 3473 3858 3875 5327 5413
Size: 315 504 315 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 504 385 367 346 315 315
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2982 2993 3292 4086 4106 2982
Size: 11 299 794 20 4652
Fragments arranged by size:
4652 794 299 20 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3223 4112 5741
Size: 54 1425 50 275 18 1393 889 1629
Cuts at: 5741 8
Size: 43
Fragments arranged by size:
1629 1425 1393 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2121 2166 2181 2190 2211 3706 1353
Size: 750 18 45 15 9 21 1495 3423
Fragments arranged by size:
3423 1495 750 45 21 18 15 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2481 2546 2723 3017 3839 4436 5223
Size: 393 849 65 177 294 822 597 787
Cuts at: 5223 5491 1239
Size: 268 1524
Fragments arranged by size:
1524 849 822 787 597 393 294 268
177 65
EcoO109I rG'GnC_Cy
Cuts at: 2707 2707
Size: 5776
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2677 4923 1678
Size: 999 2246 2531
Fragments arranged by size:
2531 2246 999
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2404 2436 2454 2486 2828
Size: 32 135 32 832 32 18 32 342
Cuts at: 2828 2860 4417 4449 4491 4523 1373
Size: 32 1557 32 42 32 2626
Fragments arranged by size:
2626 1557 832 342 135 42 32 32
32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 2920 3120 3366 669
Size: 988 281 982 200 246 3079
Fragments arranged by size:
3079 988 982 281 246 200
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 2920 3366 2920
Size: 446 5330
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2309 4890 5074 1305
Size: 1004 2581 184 2007
Fragments arranged by size:
2581 2007 1004 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3734 4093 4252 518
Size: 648 2568 359 159 2042
Fragments arranged by size:
2568 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3192 3869 5017 5262 1606
Size: 1586 677 1148 245 2120
Fragments arranged by size:
2120 1586 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2398 3134 4679 1132
Size: 97 1169 736 1545 2229
Fragments arranged by size:
2229 1545 1169 736 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2717 1108
Size: 1609 4167
PfoI T'CCnGG_A
Cuts at: 2458 2458
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2707 2707
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 2571 3346 2571
Size: 775 5001
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2078 2110 2078
Size: 32 5744
PstI C_TGCA'G
Cuts at: 830 3128 830
Size: 2298 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2621 4024 5736 2621
Size: 1403 1712 2661
Fragments arranged by size:
2661 1712 1403
SfcI C'TryA_G
Cuts at: 826 1045 2059 3124 4259 4940 5131 826
Size: 219 1014 1065 1135 681 191 1471
Fragments arranged by size:
1471 1135 1065 1014 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 2613 3103 1872
Size: 741 490 4545
Fragments arranged by size:
4545 741 490
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4781 5043 820
Size: 197 926 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 4633
StyI C'CwwG_G
Cuts at: 513 1065 2370 2934 3215 513
Size: 552 1305 564 281 3074
Fragments arranged by size:
3074 1305 564 552 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 2619 4022
Size: 274 80 33 51 1443 420 222 1403
Cuts at: 4022 5734 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1403 420 274 222 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2781 2813 5736 5768 2781
Size: 32 2923 32 2789
Fragments arranged by size:
2923 2789 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 2611 3101 1870
Size: 741 490 4545
Fragments arranged by size:
4545 741 490
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI