Restriction map for
pFN21AE6369
(Circular) (Six-base) MAPSORT of: pFN21AE6369.seq Check: 1605 from: 1 to: 5749
pFN21AE6369 5749 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2463 2752 3092 1937
Size: 526 289 340 4594
Fragments arranged by size:
4594 526 340 289
Acc65I G'GTAC_C
Cuts at: 2065 3070 2065
Size: 1005 4744
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 5196 5464 1632
Size: 2180 1384 268 1917
Fragments arranged by size:
2180 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2059 1184
Size: 875 4874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2686 2718 2686
Size: 32 5717
AlwNI CAG_nnn'CTG
Cuts at: 3492 5064 3492
Size: 1572 4177
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 2174 2967 3058 3284 3436 5545 1493
Size: 681 793 91 226 152 2109 1697
Fragments arranged by size:
2109 1697 793 681 226 152 91
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2606 2766 3070 4525
Size: 325 217 627 278 541 160 304 1455
Cuts at: 4525 618
Size: 1842
Fragments arranged by size:
1842 1455 627 541 325 304 278 217
160
BanII G_rGCy'C
Cuts at: 1391 2853 3068 1391
Size: 1462 215 4072
Fragments arranged by size:
4072 1462 215
BbeI G_GCGC'C
Cuts at: 2610 2610
Size: 5749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2187 3810 4966 1790
Size: 397 1623 1156 2573
Fragments arranged by size:
2573 1623 1156 397
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2346 4409 1239
Size: 1034 73 2063 2579
Fragments arranged by size:
2579 2063 1034 73
Bpu10I CC'TnA_GC
Cuts at: 2378 2378
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2607 3556 3938 4573
Size: 53 83 186 936 1074 949 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1451 1074 949 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2987 3017 1178
Size: 30 165 30 1584 30 3910
Fragments arranged by size:
3910 1584 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2861 1186
Size: 1675 4074
BseYI C'CCAG_C
Cuts at: 4952 5448 4952
Size: 496 5253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2198 3199 3960 4988 1540
Size: 275 186 197 1001 761 1028 2301
Fragments arranged by size:
2301 1028 1001 761 275 197 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2625 2853 3810 3895 4966 2307
Size: 318 228 957 85 1071 3090
Fragments arranged by size:
3090 1071 957 318 228 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2307 2625 2853 3068 3810 3895
Size: 399 397 120 318 228 215 742 85
Cuts at: 3895 4966 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 318 228 215
120 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3104 844
Size: 2260 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2287 2875 3636 1394
Size: 429 464 588 761 3507
Fragments arranged by size:
3507 761 588 464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2667 4244 4418 65
Size: 1183 1419 1577 174 1396
Fragments arranged by size:
1577 1419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4393 4580 1700
Size: 110 2583 187 2869
Fragments arranged by size:
2869 2583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2347 2128
Size: 219 5530
BstYI r'GATC_y
Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744
Size: 1107 367 385 17 1452 86 12 346
Cuts at: 5744 1972
Size: 1977
Fragments arranged by size:
1977 1452 1107 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2844 2844
Size: 5749
BtgI C'CryG_G
Cuts at: 513 1065 2148 2198 513
Size: 552 1083 50 4064
Fragments arranged by size:
4064 1083 552 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2584 487
Size: 683 56 1358 3652
Fragments arranged by size:
3652 1358 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 3265 4059 4079 2405
Size: 860 794 20 4075
Fragments arranged by size:
4075 860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2392 2427 529
Size: 35 1828 35 3851
Fragments arranged by size:
3851 1828 35 35
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2381 4756 809
Size: 507 1065 2375 1802
Fragments arranged by size:
2375 1802 1065 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2195 3196 4085
Size: 54 1425 50 275 18 365 1001 889
Cuts at: 4085 5714 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1001 889 365 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2195 3196 1537
Size: 275 383 1001 4090
Fragments arranged by size:
4090 1001 383 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3679 1353
Size: 2326 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2851 2851
Size: 5749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2346 3812 4409 5196 5464 1239
Size: 393 641 73 1466 597 787 268 1524
Fragments arranged by size:
1524 1466 787 641 597 393 268 73
EcoNI CCTnn'n_nnAGG
Cuts at: 2761 2761
Size: 5749
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2610 2661 4896 1678
Size: 932 51 2235 2531
Fragments arranged by size:
2531 2235 932 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2656 2688 2987 3019 4390
Size: 32 135 32 1084 32 299 32 1371
Cuts at: 4390 4422 4464 4496 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1371 1084 299 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2464 3093 3339 669
Size: 988 281 526 629 246 3079
Fragments arranged by size:
3079 988 629 526 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KasI G'GCGC_C
Cuts at: 2606 2606
Size: 5749
KpnI G_GTAC'C
Cuts at: 2069 3074 2069
Size: 1005 4744
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2617 4863 5047 1305
Size: 1312 2246 184 2007
Fragments arranged by size:
2246 2007 1312 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 2630 3707 4066 4225 518
Size: 648 1374 90 1077 359 159 2042
Fragments arranged by size:
2042 1374 1077 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2022 2431 3165 3842 4990 5235 1606
Size: 416 409 734 677 1148 245 2120
Fragments arranged by size:
2120 1148 734 677 416 409 245
NarI GG'CG_CC
Cuts at: 2607 2607
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 2148 513
Size: 552 1083 4114
Fragments arranged by size:
4114 1083 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2400 3107 4652 1132
Size: 97 1171 707 1545 2229
Fragments arranged by size:
2229 1545 1171 707 97
PasI CC'CwG_GG
Cuts at: 1348 2722 1348
Size: 1374 4375
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2457 2682 1108
Size: 1349 225 4175
Fragments arranged by size:
4175 1349 225
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3015 3319 3015
Size: 304 5445
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2022 2022
Size: 5749
SacI G_AGCT'C
Cuts at: 2853 2853
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 2462 3091 1936
Size: 526 629 4594
Fragments arranged by size:
4594 629 526
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2881 3097 4232 4913 5104 826
Size: 219 1836 216 1135 681 191 1471
Fragments arranged by size:
1836 1471 1135 681 219 216 191
SfoI GGC'GCC
Cuts at: 2608 2608
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 2646 2700 2913 3673 5
Size: 47 2594 54 213 760 2081
Fragments arranged by size:
2594 2081 760 213 54 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2133 2148 3188 513
Size: 552 1068 15 1040 3074
Fragments arranged by size:
3074 1068 1040 552 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2145 2177 2913 2945 5709 5741 2145
Size: 32 736 32 2764 32 2153
Fragments arranged by size:
2764 2153 736 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI