Restriction map for
pFN21AE6367
(Circular) (Six-base) MAPSORT of: pFN21AE6367.seq Check: 510 from: 1 to: 5785
pFN21AE6367 5785 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 2107 3912 4285 1563
Size: 544 1805 373 3063
Fragments arranged by size:
3063 1805 544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2209 2492 2517 2893 3848 5232 5500 1632
Size: 577 283 25 376 955 1384 268 1917
Fragments arranged by size:
1917 1384 955 577 376 283 268 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2396 3528 5100 2396
Size: 1132 1572 3081
Fragments arranged by size:
3081 1572 1132
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2451 2557 3010 3094 3320 3472 5581 1493
Size: 958 106 453 84 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 453 226 152 106 84
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2853 3106 4561 618
Size: 325 217 627 1066 253 1455 1842
Fragments arranged by size:
1842 1455 1066 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 3104 1391
Size: 1713 4072
BbeI G_GCGC'C
Cuts at: 2857 2857
Size: 5785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3976 4010 1903
Size: 34 50 34 1955 34 3678
Fragments arranged by size:
3678 1955 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 3846 5002 1790
Size: 2056 1156 2573
Fragments arranged by size:
2573 2056 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 3516 4474
Size: 416 53 83 399 24 640 1459 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1459 958 640 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2396 2396
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2967 2994 4445 1239
Size: 1728 27 1451 2579
Fragments arranged by size:
2579 1728 1451 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2389 2651 3910 4775 5073 2389
Size: 262 1259 865 298 3101
Fragments arranged by size:
3101 1259 865 298 262
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2854 3592 3974 4609
Size: 53 83 186 936 1321 738 382 635
Cuts at: 4609 275
Size: 1451
Fragments arranged by size:
1451 1321 936 738 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2236 2266 1178
Size: 30 165 30 833 30 4697
Fragments arranged by size:
4697 833 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5785
BseYI C'CCAG_C
Cuts at: 2096 4988 5484 2096
Size: 2892 496 2397
Fragments arranged by size:
2892 2397 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsmI GAATG_Cn'
Cuts at: 2164 3296 3389 2164
Size: 1132 93 4560
Fragments arranged by size:
4560 1132 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3104 3846 3931 5002 1391
Size: 399 1314 742 85 1071 2174
Fragments arranged by size:
2174 1314 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3140 844
Size: 2296 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2850 4429 4616 1700
Size: 110 1040 1579 187 2869
Fragments arranged by size:
2869 1579 1040 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 2551 2996 3845 4857 2551
Size: 445 849 1012 3479
Fragments arranged by size:
3479 1012 849 445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2890 794
Size: 2096 3689
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2178 2836 2178
Size: 658 5127
BstYI r'GATC_y
Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780
Size: 1143 367 385 17 1452 86 12 346
Cuts at: 5780 1972
Size: 1977
Fragments arranged by size:
1977 1452 1143 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2196 2196
Size: 5785
BtgI C'CryG_G
Cuts at: 513 1065 3024 513
Size: 552 1959 3274
Fragments arranged by size:
3274 1959 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2629 2794 2900 3301 4095 4115 2629
Size: 165 106 401 794 20 4299
Fragments arranged by size:
4299 794 401 165 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2209 2492 2517 2893 2967 2994 3848
Size: 393 577 283 25 376 74 27 854
Cuts at: 3848 4445 5232 5500 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 854 787 597 577 393 376 283
268 74 27 25
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2857 4932 1678
Size: 1179 2075 2531
Fragments arranged by size:
2531 2075 1179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2236 2268 2942 2974 4426
Size: 32 135 32 664 32 674 32 1452
Cuts at: 4426 4458 4500 4532 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1452 674 664 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3129 3375 669
Size: 988 281 1191 246 3079
Fragments arranged by size:
3079 1191 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KasI G'GCGC_C
Cuts at: 2853 2853
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2853 4899 5083 1305
Size: 1548 2046 184 2007
Fragments arranged by size:
2046 2007 1548 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3743 4102 4261 518
Size: 648 2577 359 159 2042
Fragments arranged by size:
2577 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3201 3878 5026 5271 1606
Size: 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 245
NarI GG'CG_CC
Cuts at: 2854 2854
Size: 5785
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5233
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2641 2814 3143 4688 1132
Size: 97 1412 173 329 1545 2229
Fragments arranged by size:
2229 1545 1412 329 173 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 3133 4268 4949 5140 826
Size: 219 2088 1135 681 191 1471
Fragments arranged by size:
2088 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2855 2855
Size: 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2368 2666 3889 4790 5052 820
Size: 197 926 425 298 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 425 298 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 2726 152
Size: 2574 3211
SphI G_CATG'C
Cuts at: 1132 2641 2814 3143 1132
Size: 1509 173 329 3774
Fragments arranged by size:
3774 1509 329 173
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 2576 1517
Size: 1059 4726
StyI C'CwwG_G
Cuts at: 513 1065 3224 513
Size: 552 2159 3074
Fragments arranged by size:
3074 2159 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 5743 96
Size: 274 80 33 51 1443 2054 1712 138
Fragments arranged by size:
2054 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2550 2550
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 2391 3914 2391
Size: 1523 4262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI