Restriction map for
pFN21AE6366
(Circular) (Six-base) MAPSORT of: pFN21AE6366.seq Check: 4639 from: 1 to: 6004
pFN21AE6366 6004 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 3347 1937
Size: 823 587 4594
Fragments arranged by size:
4594 823 587
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 3057 4067 5451 5719 1632
Size: 1038 387 1010 1384 268 1917
Fragments arranged by size:
1917 1384 1038 1010 387 268
AfeI AGC'GCT
Cuts at: 2921 2921
Size: 6004
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 2494 4903 5880 1203
Size: 1291 2409 977 1327
Fragments arranged by size:
2409 1327 1291 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 3747 5319 3747
Size: 1572 4432
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2243 3204 3313 3539 3691 5800 1493
Size: 750 961 109 226 152 2109 1697
Fragments arranged by size:
2109 1697 961 750 226 152 109
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2928 3329 1870
Size: 73 985 401 4545
Fragments arranged by size:
4545 985 401 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 4780 618
Size: 325 217 627 1538 1455 1842
Fragments arranged by size:
1842 1538 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2682 3323 1391
Size: 1291 641 4072
Fragments arranged by size:
4072 1291 641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 928
Size: 971 495 4538
Fragments arranged by size:
4538 971 495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 2508 1548
Size: 960 5044
BfrBI ATG'CAT
Cuts at: 3164 3164
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2963 3179 4615
Size: 107 122 71 631 605 1291 216 1436
Cuts at: 4615 136
Size: 1525
Fragments arranged by size:
1525 1436 1291 631 605 216 122 107
71
BglII A'GATC_T
Cuts at: 2081 5999 2081
Size: 3918 2086
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 4065 5221 1790
Size: 2275 1156 2573
Fragments arranged by size:
2573 2275 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3735 4693 442
Size: 416 53 83 399 24 2318 958 1753
Fragments arranged by size:
2318 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4664 1239
Size: 3425 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4667 882
Size: 617 3168 2219
Fragments arranged by size:
3168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2442 3795 493
Size: 711 1238 1353 2702
Fragments arranged by size:
2702 1353 1238 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2952 3811 4193 4828
Size: 53 83 186 936 1419 859 382 635
Cuts at: 4828 275
Size: 1451
Fragments arranged by size:
1451 1419 936 859 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5779
Fragments arranged by size:
5779 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 2948 1186
Size: 1450 312 4242
Fragments arranged by size:
4242 1450 312
BseYI C'CCAG_C
Cuts at: 2200 5207 5703 2200
Size: 3007 496 2501
Fragments arranged by size:
3007 2501 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2978 1163
Size: 573 1242 4189
Fragments arranged by size:
4189 1242 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4065 4150 5221 4065
Size: 85 1071 4848
Fragments arranged by size:
4848 1071 85
BsmI GAATG_Cn'
Cuts at: 2032 3515 3608 2032
Size: 1483 93 4428
Fragments arranged by size:
4428 1483 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2682 3323 4065 4150 5221 1391
Size: 399 892 641 742 85 1071 2174
Fragments arranged by size:
2174 1071 892 742 641 399 85
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 2223 2730 2854 3082 3094 3893 5623 2223
Size: 507 124 228 12 799 1730 2604
Fragments arranged by size:
2604 1730 799 507 228 124 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3359 844
Size: 2515 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2370 4499 4673 65
Size: 1183 1122 2129 174 1396
Fragments arranged by size:
2129 1396 1183 1122 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2948 4648 4835 1700
Size: 110 1138 1700 187 2869
Fragments arranged by size:
2869 1700 1138 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3023 3257 794
Size: 2229 234 3541
Fragments arranged by size:
3541 2229 234
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2081 3334 3701 4086 4103 5555 5641 5653
Size: 109 1253 367 385 17 1452 86 12
Cuts at: 5653 5999 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1253 385 367 346 109 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3021 487
Size: 683 56 1795 3470
Fragments arranged by size:
3470 1795 683 56
BtsI GCAGTG_nn'
Cuts at: 3520 4314 4334 3520
Size: 794 20 5190
Fragments arranged by size:
5190 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 2521 3308 2521
Size: 787 5217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2824 3451
Size: 54 1425 50 275 18 441 553 627
Cuts at: 3451 4340 5969 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 627 553 441 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2718 3934 1353
Size: 1365 1216 3423
Fragments arranged by size:
3423 1365 1216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2670 3057 4067 4664 5451 5719 1239
Size: 393 1038 387 1010 597 787 268 1524
Fragments arranged by size:
1524 1038 1010 787 597 393 387 268
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 2923 5151 1678
Size: 1245 2228 2531
Fragments arranged by size:
2531 2228 1245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 4645 4677 4719
Size: 32 135 32 494 32 2547 32 42
Cuts at: 4719 4751 1373
Size: 32 2626
Fragments arranged by size:
2626 2547 494 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3018 3603 3018
Size: 585 5419
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5118 5302 1305
Size: 3813 184 2007
Fragments arranged by size:
3813 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 2322 2349 2853 3962 4321 4480
Size: 648 934 222 27 504 1109 359 159
Cuts at: 4480 518
Size: 2042
Fragments arranged by size:
2042 1109 934 648 504 359 222 159
27
MspA1I CmG'CkG
Cuts at: 1606 2023 3420 4097 5245 5490 1606
Size: 417 1397 677 1148 245 2120
Fragments arranged by size:
2120 1397 1148 677 417 245
NaeI GCC'GGC
Cuts at: 2950 2950
Size: 6004
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NgoMIV G'CCGG_C
Cuts at: 2948 2948
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NsiI A_TGCA'T
Cuts at: 3166 3166
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2498 3362 4907 1132
Size: 97 1269 864 1545 2229
Fragments arranged by size:
2229 1545 1269 864 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 2494 4903 2494
Size: 2409 3595
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PmlI CAC'GTG
Cuts at: 2442 2442
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2484 2516 4073 4105 5586 5618 2484
Size: 32 1557 32 1481 32 2870
Fragments arranged by size:
2870 1557 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3159 3574 3159
Size: 415 5589
PstI C_TGCA'G
Cuts at: 830 2710 3356 830
Size: 1880 646 3478
Fragments arranged by size:
3478 1880 646
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 3061 4252 5964 3061
Size: 1191 1712 3101
Fragments arranged by size:
3101 1712 1191
SfcI C'TryA_G
Cuts at: 826 1045 2706 3352 4487 5168 5359 826
Size: 219 1661 646 1135 681 191 1471
Fragments arranged by size:
1661 1471 1135 681 646 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 2469 1517
Size: 952 5052
StyI C'CwwG_G
Cuts at: 513 1065 2004 3443 513
Size: 552 939 1439 3074
Fragments arranged by size:
3074 1439 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2972 4031 4048 4201 4386 4831 325
Size: 1255 1392 1059 17 153 185 445 1498
Fragments arranged by size:
1498 1392 1255 1059 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3059 4250 5962
Size: 274 80 33 51 1443 1082 1191 1712
Cuts at: 5962 96
Size: 138
Fragments arranged by size:
1712 1443 1191 1082 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2272 2304 5964 5996 2272
Size: 32 3660 32 2280
Fragments arranged by size:
3660 2280 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 4133 4133
Size: 6004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI