Restriction map for
pFN21AE6363
(Circular) (Six-base) MAPSORT of: pFN21AE6363.seq Check: 135 from: 1 to: 5977
pFN21AE6363 5977 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2057 3320 1937
Size: 120 1263 4594
Fragments arranged by size:
4594 1263 120
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2664 2786 2843 2996 3046 4040 5424
Size: 818 214 122 57 153 50 994 1384
Cuts at: 5424 5692 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 994 818 268 214 153 122
57 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2991 4876 5853 1203
Size: 1788 1885 977 1327
Fragments arranged by size:
1885 1788 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 3038 1166
Size: 1872 4105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 5945
AlwNI CAG_nnn'CTG
Cuts at: 3044 3720 5292 3044
Size: 676 1572 3729
Fragments arranged by size:
3729 1572 676
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2248 2273 2386 3077 3286 3512 3664 5773
Size: 755 25 113 691 209 226 152 2109
Cuts at: 5773 1493
Size: 1697
Fragments arranged by size:
2109 1697 755 691 226 209 152 113
25
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 1870
Size: 73 1359 4545
Fragments arranged by size:
4545 1359 73
AvrII C'CTAG_G
Cuts at: 3158 3158
Size: 5977
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3261 3294 3261
Size: 33 5944
BamHI G'GATC_C
Cuts at: 2448 3171 3307 3674 2448
Size: 723 136 367 4751
Fragments arranged by size:
4751 723 367 136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 3298 4753 618
Size: 325 217 627 285 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 325 285 217
BanII G_rGCy'C
Cuts at: 1391 3296 1391
Size: 1905 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2329 928
Size: 971 430 4576
Fragments arranged by size:
4576 971 430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 2875 1548
Size: 1327 4650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3178 5972 3178
Size: 2794 3183
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 4038 5194 1790
Size: 2248 1156 2573
Fragments arranged by size:
2573 2248 1156
BmgBI CAC'GTC
Cuts at: 1318 2537 1318
Size: 1219 4758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4637 1239
Size: 3398 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2080 3123 4102 4967 5265 2080
Size: 1043 979 865 298 2792
Fragments arranged by size:
2792 1043 979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2437 3784 4166 4801
Size: 53 83 186 936 904 1347 382 635
Cuts at: 4801 275
Size: 1451
Fragments arranged by size:
1451 1347 936 904 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3145 4410 4808 5082 5229 5640 3145
Size: 1265 398 274 147 411 3482
Fragments arranged by size:
3482 1265 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2857 2887 1178
Size: 30 165 30 1454 30 4268
Fragments arranged by size:
4268 1454 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2878 1186
Size: 1692 4285
BseYI C'CCAG_C
Cuts at: 5180 5676 5180
Size: 496 5481
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 4038 4123 5194 2177
Size: 1861 85 1071 2960
Fragments arranged by size:
2960 1861 1071 85
BsmI GAATG_Cn'
Cuts at: 2829 2945 3488 3581 2829
Size: 116 543 93 5225
Fragments arranged by size:
5225 543 116 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 3296 4038 4123 5194 1391
Size: 399 387 1119 742 85 1071 2174
Fragments arranged by size:
2174 1119 1071 742 399 387 85
BspEI T'CCGG_A
Cuts at: 3145 5640 3145
Size: 2495 3482
BspHI T'CATG_A
Cuts at: 2286 3866 5596 2286
Size: 1580 1730 2667
Fragments arranged by size:
2667 1730 1580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2082 4472 4646 65
Size: 1183 834 2390 174 1396
Fragments arranged by size:
2390 1396 1183 834 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 2618 96
Size: 2522 3455
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 3016 1184
Size: 1832 4145
BstYI r'GATC_y
Cuts at: 1972 2448 3171 3178 3307 3674 4059 4076 5528
Size: 476 723 7 129 367 385 17 1452
Cuts at: 5528 5614 5626 5972 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 723 476 385 367 346 129
86 17 12 7
BstZ17I GTA'TAC
Cuts at: 2058 2058
Size: 5977
BtgI C'CryG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 4123
Fragments arranged by size:
4123 939 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 3493 4287 4307 3493
Size: 794 20 5163
Fragments arranged by size:
5163 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2905 3424 4313
Size: 54 1425 50 275 18 1075 519 889
Cuts at: 4313 5942 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1075 889 519 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 2426 3907 1353
Size: 1062 11 1481 3423
Fragments arranged by size:
3423 1481 1062 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2664 2786 2843 2996 3046 4040
Size: 393 818 214 122 57 153 50 994
Cuts at: 4040 4637 5424 5692 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 994 818 787 597 393 268 214
153 122 57 50
EcoO109I rG'GnC_Cy
Cuts at: 2953 3066 2953
Size: 113 5864
EcoRI G'AATT_C
Cuts at: 2273 3286 3664 2273
Size: 1013 378 4586
Fragments arranged by size:
4586 1013 378
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 5124 1678
Size: 3446 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3163 3195 4618 4650 4692
Size: 32 135 32 1591 32 1423 32 42
Cuts at: 4692 4724 1373
Size: 32 2626
Fragments arranged by size:
2626 1591 1423 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2049 3321 3567 669
Size: 988 281 111 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 2614 3576 2614
Size: 962 5015
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2180 5091 5275 1305
Size: 875 2911 184 2007
Fragments arranged by size:
2911 2007 875 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2907 10
Size: 54 1425 343 1075 3080
Fragments arranged by size:
3080 1425 1075 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2550 3038 3935 4294 4453 518
Size: 648 1384 488 897 359 159 2042
Fragments arranged by size:
2042 1384 897 648 488 359 159
MspA1I CmG'CkG
Cuts at: 1606 3393 4070 5218 5463 1606
Size: 1787 677 1148 245 2120
Fragments arranged by size:
2120 1787 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 4123
Fragments arranged by size:
4123 939 552 363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2995 3335 4880 1132
Size: 97 1766 340 1545 2229
Fragments arranged by size:
2229 1766 1545 340 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 2991 4876 2991
Size: 1885 4092
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PfoI T'CCnGG_A
Cuts at: 2264 2319 2982 2264
Size: 55 663 5259
Fragments arranged by size:
5259 663 55
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 2225 2399 3123 3547 2225
Size: 174 724 424 4655
Fragments arranged by size:
4655 724 424 174
PstI C_TGCA'G
Cuts at: 830 3329 830
Size: 2499 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2415 2415
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2020 3325 4460 5141 5332 826
Size: 219 975 1305 1135 681 191 1471
Fragments arranged by size:
1471 1305 1135 975 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2095 3138 4081 4982 5244 820
Size: 197 926 152 1043 943 901 262 1553
Fragments arranged by size:
1553 1043 943 926 901 262 197 152
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 2110 152
Size: 1958 4019
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 2367 3158 3416 513
Size: 552 939 363 791 258 3074
Fragments arranged by size:
3074 939 791 552 363 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4004 4021 4174 4359 4804 325
Size: 1255 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2618 4223 5935
Size: 274 80 33 51 1443 641 1605 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1712 1605 1443 641 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2857 2889 5937 5969 2000
Size: 32 825 32 3048 32 2008
Fragments arranged by size:
3048 2008 825 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 2273 4106 2273
Size: 1833 4144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI