Restriction map for
pFN21AE6362
(Circular) (Six-base) MAPSORT of: pFN21AE6362.seq Check: 1661 from: 1 to: 5716
pFN21AE6362 5716 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2643 3059 1937
Size: 706 416 4594
Fragments arranged by size:
4594 706 416
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3779 5163 5431 1632
Size: 1185 962 1384 268 1917
Fragments arranged by size:
1917 1384 1185 962 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 2646 4615 5592 1203
Size: 1443 1969 977 1327
Fragments arranged by size:
1969 1443 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaLI G'TGCA_C
Cuts at: 2212 3773 4929 2212
Size: 1561 1156 2999
Fragments arranged by size:
2999 1561 1156
ApoI r'AATT_y
Cuts at: 1493 2967 3025 3251 3403 5512 1493
Size: 1474 58 226 152 2109 1697
Fragments arranged by size:
2109 1697 1474 226 152 58
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2197 3041 1870
Size: 73 254 844 4545
Fragments arranged by size:
4545 844 254 73
AvrII C'CTAG_G
Cuts at: 2047 2047
Size: 5716
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2356 2902 3037 4492 618
Size: 325 217 627 569 546 135 1455 1842
Fragments arranged by size:
1842 1455 627 569 546 325 217 135
BanII G_rGCy'C
Cuts at: 1391 2085 3035 1391
Size: 694 950 4072
Fragments arranged by size:
4072 950 694
BbeI G_GCGC'C
Cuts at: 2360 2360
Size: 5716
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2794 928
Size: 971 895 3850
Fragments arranged by size:
3850 971 895
BbvCI CC'TCA_GC
Cuts at: 2782 2782
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2250 3608 4818 2250
Size: 1358 1210 3148
Fragments arranged by size:
3148 1358 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2438 2580 4327
Size: 107 122 71 631 605 766 142 1747
Cuts at: 4327 136
Size: 1525
Fragments arranged by size:
1747 1525 766 631 605 142 122 107
71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 2216 3777 4933 1790
Size: 426 1561 1156 2573
Fragments arranged by size:
2573 1561 1156 426
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2821 3447 4405
Size: 416 53 83 399 24 1404 626 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1404 958 626 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2756 1056
Size: 1700 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4376 1239
Size: 3137 2579
Bpu10I CC'TnA_GC
Cuts at: 2782 2782
Size: 5716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 2357 2381 3523
Size: 53 83 186 936 528 296 24 1142
Cuts at: 3523 3905 4540 275
Size: 382 635 1451
Fragments arranged by size:
1451 1142 936 635 528 382 296 186
83 53 24
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 4866
BseYI C'CCAG_C
Cuts at: 2726 4919 5415 2726
Size: 2193 496 3027
Fragments arranged by size:
3027 2193 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 3777 3862 4933 2216
Size: 1561 85 1071 2999
Fragments arranged by size:
2999 1561 1071 85
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
BsmBI CGTCTCn'nnnn_
Cuts at: 2052 2052
Size: 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2216 3035 3777 3862 4933 1391
Size: 399 295 131 819 742 85 1071 2174
Fragments arranged by size:
2174 1071 819 742 399 295 131 85
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2341 3071 844
Size: 1497 730 3489
Fragments arranged by size:
3489 1497 730
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2707 2895 4211 4385 65
Size: 1183 1459 188 1316 174 1396
Fragments arranged by size:
1459 1396 1316 1183 188 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4360 4547 1700
Size: 110 2550 187 2869
Fragments arranged by size:
2869 2550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 2618 3776 4788 2618
Size: 1158 1012 3546
Fragments arranged by size:
3546 1158 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 2155 3046 3413 3798 3815 5267 5353 5365
Size: 183 891 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 891 385 367 346 183 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2415 2415
Size: 5716
BtgI C'CryG_G
Cuts at: 513 1065 2361 513
Size: 552 1296 3868
Fragments arranged by size:
3868 1296 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 3232 4026 4046 3232
Size: 794 20 4902
Fragments arranged by size:
4902 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 2818 3020 2818
Size: 202 5514
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3163 4052 5681
Size: 54 1425 50 275 18 1333 889 1629
Cuts at: 5681 8
Size: 43
Fragments arranged by size:
1629 1425 1333 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2955 3646 1353
Size: 1602 691 3423
Fragments arranged by size:
3423 1602 691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 4303 4677 4823 387
Size: 1064 138 672 2042 374 146 1280
Fragments arranged by size:
2042 1280 1064 672 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2817 3779 4376 5163 5431 1239
Size: 393 1185 962 597 787 268 1524
Fragments arranged by size:
1524 1185 962 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2015 2015
Size: 5716
EcoO109I rG'GnC_Cy
Cuts at: 2778 2823 2778
Size: 45 5671
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2493 2493
Size: 5716
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2113 2360 4863 1678
Size: 435 247 2503 2531
Fragments arranged by size:
2531 2503 435 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 2099 2131 2398
Size: 32 135 32 477 32 18 32 267
Cuts at: 2398 2430 2770 2802 4357 4389 4431 4463 1373
Size: 32 340 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 477 340 267 135 42 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2265 2321 748
Size: 1517 56 4143
Fragments arranged by size:
4143 1517 56
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KasI G'GCGC_C
Cuts at: 2356 2356
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3002 4830 5014 1305
Size: 1697 1828 184 2007
Fragments arranged by size:
2007 1828 1697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3674 4033 4192 518
Size: 648 2508 359 159 2042
Fragments arranged by size:
2508 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3132 3809 4957 5202 1606
Size: 1526 677 1148 245 2120
Fragments arranged by size:
2120 1526 1148 677 245
NarI GG'CG_CC
Cuts at: 2357 2357
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 2752 1052
Size: 1700 4016
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2650 3074 4619 1132
Size: 97 1421 424 1545 2229
Fragments arranged by size:
2229 1545 1421 424 97
PasI CC'CwG_GG
Cuts at: 1348 2846 1348
Size: 1498 4218
PciI A'CATG_T
Cuts at: 2646 4615 2646
Size: 1969 3747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2933 1108
Size: 1825 3891
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2778 2823 2778
Size: 45 5671
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PspXI vC'TCGA_Gb
Cuts at: 2197 2197
Size: 5716
PstI C_TGCA'G
Cuts at: 830 2338 3068 830
Size: 1508 730 3478
Fragments arranged by size:
3478 1508 730
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 2948 3964 5676 2948
Size: 1016 1712 2988
Fragments arranged by size:
2988 1712 1016
SfcI C'TryA_G
Cuts at: 826 1045 2334 2950 3064 4199 4880 5071 826
Size: 219 1289 616 114 1135 681 191 1471
Fragments arranged by size:
1471 1289 1135 681 616 219 191 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 2580 2580
Size: 5716
SfoI GGC'GCC
Cuts at: 2358 2358
Size: 5716
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2197 3820 4721 4983 820
Size: 197 926 254 1623 901 262 1553
Fragments arranged by size:
1623 1553 926 901 262 254 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2047 2585 3155 513
Size: 552 982 538 570 3074
Fragments arranged by size:
3074 982 570 552 538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3743 3760 3913 4098 4543 325
Size: 1255 2163 17 153 185 445 1498
Fragments arranged by size:
2163 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2876 2946 3962
Size: 274 80 33 51 1443 899 70 1016
Cuts at: 3962 5674 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1016 899 274 138 80 70
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2886 2918 5676 5708 2886
Size: 32 2758 32 2894
Fragments arranged by size:
2894 2758 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2063 1316
Size: 747 4969
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2567 2853 2567
Size: 286 5430
XhoI C'TCGA_G
Cuts at: 1943 2197 1943
Size: 254 5462
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SrfI
SwaI