Restriction map for
pFN21AE6359
(Circular) (Six-base) MAPSORT of: pFN21AE6359.seq Check: 7857 from: 1 to: 5890
pFN21AE6359 5890 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 3233 1937
Size: 912 384 4594
Fragments arranged by size:
4594 912 384
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 3953 5337 5605 1632
Size: 918 1403 1384 268 1917
Fragments arranged by size:
1917 1403 1384 918 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 2185 4789 5766 1203
Size: 982 2604 977 1327
Fragments arranged by size:
2604 1327 982 977
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 3633 5205 3633
Size: 1572 4318
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 2392 2516 2768 3199 3425 3577 5686 1493
Size: 899 124 252 431 226 152 2109 1697
Fragments arranged by size:
2109 1697 899 431 252 226 152 124
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
BamHI G'GATC_C
Cuts at: 2418 2884 3220 3587 2418
Size: 466 336 367 4721
Fragments arranged by size:
4721 466 367 336
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3211 4666 618
Size: 325 217 627 1424 1455 1842
Fragments arranged by size:
1842 1455 1424 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3209 1391
Size: 1818 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2441 3006 928
Size: 971 542 565 3812
Fragments arranged by size:
3812 971 565 542
BbvCI CC'TCA_GC
Cuts at: 2024 2024
Size: 5890
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4501 136
Size: 107 122 71 631 605 2829 1525
Fragments arranged by size:
2829 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 3307 3307
Size: 5890
Bme1580I G_kGCm'C
Cuts at: 1790 3133 3951 5107 1790
Size: 1343 818 1156 2573
Fragments arranged by size:
2573 1343 1156 818
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 2421 3621
Size: 416 53 83 399 24 732 272 1200
Cuts at: 3621 4579 442
Size: 958 1753
Fragments arranged by size:
1753 1200 958 732 416 399 272 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2796 1056
Size: 1740 4150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4550 1239
Size: 3311 2579
Bpu10I CC'TnA_GC
Cuts at: 2024 2024
Size: 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4015 4880 5178 4015
Size: 865 298 4727
Fragments arranged by size:
4727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4553 882
Size: 617 3054 2219
Fragments arranged by size:
3054 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 5884 5884
Size: 5890
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2337 2367 1178
Size: 30 165 30 934 30 4701
Fragments arranged by size:
4701 934 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2424 1186
Size: 1238 4652
BseYI C'CCAG_C
Cuts at: 2788 5093 5589 2788
Size: 2305 496 3089
Fragments arranged by size:
3089 2305 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3340 4101 5129 1540
Size: 275 186 1339 761 1028 2301
Fragments arranged by size:
2301 1339 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3951 4036 5107 3951
Size: 85 1071 4734
Fragments arranged by size:
4734 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3133 3209 3951 4036 5107 1391
Size: 399 1343 76 742 85 1071 2174
Fragments arranged by size:
2174 1343 1071 742 399 85 76
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3245 844
Size: 2401 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 1394
Size: 429 1954 3507
Fragments arranged by size:
3507 1954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4534 4721 1700
Size: 110 2724 187 2869
Fragments arranged by size:
2869 2724 187 110
BsrGI T'GTAC_A
Cuts at: 96 2156 96
Size: 2060 3830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 3950 4962 3950
Size: 1012 4878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5890
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 2418 2884 3220 3587 3972 3989 5441 5527
Size: 446 466 336 367 385 17 1452 86
Cuts at: 5527 5539 5885 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 466 446 385 367 346 336
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4399
Fragments arranged by size:
4399 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2846 487
Size: 683 56 1620 3531
Fragments arranged by size:
3531 1620 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2079 2125
Size: 54 1425 50 275 18 177 72 46
Cuts at: 2125 3337 4226 5855 8
Size: 1212 889 1629 43
Fragments arranged by size:
1629 1425 1212 889 275 177 72 54
50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3820 1353
Size: 2467 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 3953 4550 5337 5605 1239
Size: 393 918 1403 597 787 268 1524
Fragments arranged by size:
1524 1403 918 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2819
Size: 5890
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 5037 1678
Size: 3359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2687 2719 4531 4563 4605
Size: 32 135 32 1115 32 1812 32 42
Cuts at: 4605 4637 1373
Size: 32 2626
Fragments arranged by size:
2626 1812 1115 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5890
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 2724 3489 2724
Size: 765 5125
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5004 5188 1305
Size: 3699 184 2007
Fragments arranged by size:
3699 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2127 10
Size: 54 1425 343 295 3773
Fragments arranged by size:
3773 1425 343 295 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3848 4207 4366 518
Size: 648 2682 359 159 2042
Fragments arranged by size:
2682 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3306 3983 5131 5376 1606
Size: 1700 677 1148 245 2120
Fragments arranged by size:
2120 1700 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4399
Fragments arranged by size:
4399 939 552
NdeI CA'TA_TG
Cuts at: 387 3174 387
Size: 2787 3103
NheI G'CTAG_C
Cuts at: 1052 2792 1052
Size: 1740 4150
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 2189 3248 4793 1132
Size: 97 960 1059 1545 2229
Fragments arranged by size:
2229 1545 1059 960 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5890
PciI A'CATG_T
Cuts at: 2185 4789 2185
Size: 2604 3286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2740 1108
Size: 1632 4258
PfoI T'CCnGG_A
Cuts at: 2854 2854
Size: 5890
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PstI C_TGCA'G
Cuts at: 830 3242 830
Size: 2412 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 2431 4138 5850 2431
Size: 1707 1712 2471
Fragments arranged by size:
2471 1712 1707
SfcI C'TryA_G
Cuts at: 826 1045 2304 3054 3238 4373 5054 5245 826
Size: 219 1259 750 184 1135 681 191 1471
Fragments arranged by size:
1471 1259 1135 750 681 219 191 184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3994 4895 5157 820
Size: 197 926 2051 901 262 1553
Fragments arranged by size:
2051 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 2473 3814 5
Size: 47 2421 1341 2081
Fragments arranged by size:
2421 2081 1341 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 2004 2623 3329 513
Size: 552 939 619 706 3074
Fragments arranged by size:
3074 939 706 619 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2427 3917 3934 4087 4272 4717 325
Size: 1255 847 1490 17 153 185 445 1498
Fragments arranged by size:
1498 1490 1255 847 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2156 2429 2896
Size: 274 80 33 51 1443 179 273 467
Cuts at: 2896 4136 5848 96
Size: 1240 1712 138
Fragments arranged by size:
1712 1443 1240 467 274 273 179 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2337 2369 5850 5882 2337
Size: 32 3481 32 2345
Fragments arranged by size:
3481 2345 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 3048 4019 3048
Size: 971 4919
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI