Restriction map for
pFN21AE6356
(Circular) (Six-base) MAPSORT of: pFN21AE6356.seq Check: 8467 from: 1 to: 5668
pFN21AE6356 5668 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2766 3011 1937
Size: 829 245 4594
Fragments arranged by size:
4594 829 245
Acc65I G'GTAC_C
Cuts at: 2838 2989 2838
Size: 151 5517
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 3731 5115 5383 1632
Size: 968 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 968 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 2187 4567 5544 1203
Size: 984 2380 977 1327
Fragments arranged by size:
2380 1327 984 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2952 1184
Size: 1768 3900
AlwNI CAG_nnn'CTG
Cuts at: 2834 3411 4983 2834
Size: 577 1572 3519
Fragments arranged by size:
3519 1572 577
ApaI G_GGCC'C
Cuts at: 2645 2645
Size: 5668
ApaLI G'TGCA_C
Cuts at: 2183 2276 3725 4881 2183
Size: 93 1449 1156 2970
Fragments arranged by size:
2970 1449 1156 93
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 2637 2645 2993 1870
Size: 73 657 37 8 348 4545
Fragments arranged by size:
4545 657 348 73 37 8
BamHI G'GATC_C
Cuts at: 2424 2472 2796 2998 3365 2424
Size: 48 324 202 367 4727
Fragments arranged by size:
4727 367 324 202 48
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2699 2838 2989 4444 618
Size: 325 217 627 912 139 151 1455 1842
Fragments arranged by size:
1842 1455 912 627 325 217 151 139
BanII G_rGCy'C
Cuts at: 1391 2397 2645 2828 2987 1391
Size: 1006 248 183 159 4072
Fragments arranged by size:
4072 1006 248 183 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2604 4279
Size: 107 122 71 631 605 343 589 1675
Cuts at: 4279 136
Size: 1525
Fragments arranged by size:
1675 1525 631 605 589 343 122 107
71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 2207 3085 2207
Size: 878 4790
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2187 2280 2638 2645 3729 4885 1790
Size: 259 138 93 358 7 1084 1156 2573
Fragments arranged by size:
2573 1156 1084 358 259 138 93 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2209 3399 4357
Size: 416 53 83 399 24 792 1190 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1190 958 792 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2329 2401 4328 1239
Size: 1073 17 72 1927 2579
Fragments arranged by size:
2579 1927 1073 72 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 4331 882
Size: 617 1068 1764 2219
Fragments arranged by size:
2219 1764 1068 617
BsaAI yAC'GTr
Cuts at: 493 1204 2188 2788 3459 493
Size: 711 984 600 671 2702
Fragments arranged by size:
2702 984 711 671 600
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3475 3857 4492 275
Size: 53 83 186 936 1942 382 635 1451
Fragments arranged by size:
1942 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2475 4101 4499 4773 4920 5331 2475
Size: 1626 398 274 147 411 2812
Fragments arranged by size:
2812 1626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 2290 2320 1178
Size: 30 165 30 825 30 32 30 4526
Fragments arranged by size:
4526 825 165 32 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5668
BseYI C'CCAG_C
Cuts at: 2481 2493 2727 4871 5367 2481
Size: 12 234 2144 496 2782
Fragments arranged by size:
2782 2144 496 234 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 2729 2912 1163
Size: 573 588 405 183 3919
Fragments arranged by size:
3919 588 573 405 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2090 2113 2142 2746 3118 3879
Size: 275 186 89 23 29 604 372 761
Cuts at: 3879 4907 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 604 372 275 186 89
29 23
BsiHKAI G_wGCw'C
Cuts at: 2187 2226 2280 2397 3729 3814 4885 2187
Size: 39 54 117 1332 85 1071 2970
Fragments arranged by size:
2970 1332 1071 117 85 54 39
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2187 2226 2280 2397 2638 2645
Size: 399 259 138 39 54 117 241 7
Cuts at: 2645 2828 2987 3729 3814 4885 1391
Size: 183 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 241 183 159
138 117 85 54 39 7
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 2585 3023 844
Size: 1239 502 438 3489
Fragments arranged by size:
3489 1239 502 438
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2141 2489 4312 4499 1700
Size: 110 275 56 348 1823 187 2869
Fragments arranged by size:
2869 1823 348 275 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 2772 3728 4740 2772
Size: 956 1012 3700
Fragments arranged by size:
3700 1012 956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 2485 1184
Size: 1301 4367
BstYI r'GATC_y
Cuts at: 1972 2424 2472 2796 2998 3365 3750 3767 5219
Size: 452 48 324 202 367 385 17 1452
Cuts at: 5219 5305 5317 5663 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 452 385 367 346 324 202
86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2060 2353 2060
Size: 293 5375
BtgI C'CryG_G
Cuts at: 513 1065 2004 2153 2559 513
Size: 552 939 149 406 3622
Fragments arranged by size:
3622 939 552 406 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2365 487
Size: 683 56 1139 3790
Fragments arranged by size:
3790 1139 683 56
BtsI GCAGTG_nn'
Cuts at: 2201 2236 3184 3978 3998 2201
Size: 35 948 794 20 3871
Fragments arranged by size:
3871 948 794 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2087 2110
Size: 54 1425 50 275 18 253 4 23
Cuts at: 2110 2139 2150 2743 2913 3115 4004 5633 8
Size: 29 11 593 170 202 889 1629 43
Fragments arranged by size:
1629 1425 889 593 275 253 202 170
54 50 43 29 23 18 11 4
EagI C'GGCC_G
Cuts at: 1537 1812 2087 2110 2139 2743 3115 1537
Size: 275 275 23 29 604 372 4090
Fragments arranged by size:
4090 604 372 275 275 29 23
EarI CTCTTCn'nnn_
Cuts at: 1353 2888 3598 1353
Size: 1535 710 3423
Fragments arranged by size:
3423 1535 710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 2789 4255 4629 4775 387
Size: 1064 138 570 630 1466 374 146 1280
Fragments arranged by size:
1466 1280 1064 630 570 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 2395
Size: 5668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2329 2401 2600 3731 4328 5115
Size: 393 680 17 72 199 1131 597 787
Cuts at: 5115 5383 1239
Size: 268 1524
Fragments arranged by size:
1524 1131 787 680 597 393 268 199
72 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2444 2543 2444
Size: 99 5569
EcoO109I rG'GnC_Cy
Cuts at: 2016 2250 2641 2642 2675 2016
Size: 234 391 1 33 5009
Fragments arranged by size:
5009 391 234 33 1
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FseI GG_CCGG'CC
Cuts at: 2089 2089
Size: 5668
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 4815 1678
Size: 3137 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2842 2993 2842
Size: 151 5517
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 4782 4966 1305
Size: 984 2493 184 2007
Fragments arranged by size:
2493 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2152 2915 10
Size: 54 1425 343 320 763 2763
Fragments arranged by size:
2763 1425 763 343 320 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2152 3626 3985 4144 518
Size: 648 986 1474 359 159 2042
Fragments arranged by size:
2042 1474 986 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 2373 3084 3761 4909 5154 1606
Size: 625 142 711 677 1148 245 2120
Fragments arranged by size:
2120 1148 711 677 625 245 142
NaeI GCC'GGC
Cuts at: 2087 2143 2087
Size: 56 5612
NcoI C'CATG_G
Cuts at: 513 1065 2004 2153 513
Size: 552 939 149 4028
Fragments arranged by size:
4028 939 552 149
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NgoMIV G'CCGG_C
Cuts at: 2085 2141 2085
Size: 56 5612
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 2743 3115 2743
Size: 372 5296
NspI r_CATG'y
Cuts at: 1132 1229 2629 3026 4571 1132
Size: 97 1400 397 1545 2229
Fragments arranged by size:
2229 1545 1400 397 97
PasI CC'CwG_GG
Cuts at: 1348 2468 1348
Size: 1120 4548
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2081 1108
Size: 973 4695
PfoI T'CCnGG_A
Cuts at: 2442 2442
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PmlI CAC'GTG
Cuts at: 2188 2188
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2250 2250
Size: 5668
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PspOMI G'GGCC_C
Cuts at: 2641 2641
Size: 5668
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2231 2231
Size: 5668
SacI G_AGCT'C
Cuts at: 2397 2397
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SanDI GG'GwC_CC
Cuts at: 2250 2250
Size: 5668
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SexAI A'CCwGG_T
Cuts at: 2271 2271
Size: 5668
SfcI C'TryA_G
Cuts at: 826 1045 3016 4151 4832 5023 826
Size: 219 1971 1135 681 191 1471
Fragments arranged by size:
1971 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2015 2015
Size: 5668
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2602 2639 2647 2995 1872
Size: 730 37 8 348 4545
Fragments arranged by size:
4545 730 348 37 8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 2450 1517
Size: 933 4735
StyI C'CwwG_G
Cuts at: 513 1065 2004 2011 2153 3107 513
Size: 552 939 7 142 954 3074
Fragments arranged by size:
3074 954 939 552 142 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2209 2473 2695 2946 3695 3712 3865
Size: 1255 629 264 222 251 749 17 153
Cuts at: 3865 4050 4495 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 749 629 445 264 251 222
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 2290 2322 5628 5660 2228
Size: 32 30 32 3306 32 2236
Fragments arranged by size:
3306 2236 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2600 2637 2645 2993 1870
Size: 730 37 8 348 4545
Fragments arranged by size:
4545 730 348 37 8
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI
XcmI