Restriction map for
pFN21AE6355
(Circular) (Six-base) MAPSORT of: pFN21AE6355.seq Check: 870 from: 1 to: 5752
pFN21AE6355 5752 bp
With 182 enzymes: SgfI *
July 14, 2018 22:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2384 2384
Size: 5752
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 2311 2474 2853 3003 3815 5199 5467
Size: 647 32 163 379 150 812 1384 268
Cuts at: 5467 1632
Size: 1917
Fragments arranged by size:
1917 1384 812 647 379 268 163 150
32
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 2019 4651 5628 1203
Size: 816 2632 977 1327
Fragments arranged by size:
2632 1327 977 816
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 2762 4481 2762
Size: 1719 4033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2930 3061 3287 3439 5548 1493
Size: 1437 131 226 152 2109 1697
Fragments arranged by size:
2109 1697 1437 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 3077 1870
Size: 73 1134 4545
Fragments arranged by size:
4545 1134 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2464 3014 3073 4528 618
Size: 325 217 627 677 550 59 1455 1842
Fragments arranged by size:
1842 1455 677 627 550 325 217 59
BanII G_rGCy'C
Cuts at: 1391 3004 3071 1391
Size: 1613 67 4072
Fragments arranged by size:
4072 1613 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2242 2317 928
Size: 971 343 75 4363
Fragments arranged by size:
4363 971 343 75
BbvCI CC'TCA_GC
Cuts at: 2357 2357
Size: 5752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 2580 1548
Size: 1032 4720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2182 2201
Size: 107 122 71 631 605 337 173 19
Cuts at: 2201 4363 136
Size: 2162 1525
Fragments arranged by size:
2162 1525 631 605 337 173 122 107
71 19
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2395 2469 3813 4969 1790
Size: 605 74 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 605 74
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2413 4412 1239
Size: 1086 88 1999 2579
Fragments arranged by size:
2579 1999 1086 88
Bpu10I CC'TnA_GC
Cuts at: 2357 2357
Size: 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 2582 5746 2582
Size: 3164 2588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2411 2441 1178
Size: 30 165 30 1008 30 4489
Fragments arranged by size:
4489 1008 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2671 2855 2891 1186
Size: 1216 269 184 36 4047
Fragments arranged by size:
4047 1216 269 184 36
BseYI C'CCAG_C
Cuts at: 2128 2239 2710 4955 5451 2128
Size: 111 471 2245 496 2429
Fragments arranged by size:
2429 2245 496 471 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2119 2617 2773 1163
Size: 573 383 498 156 4142
Fragments arranged by size:
4142 573 498 383 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3004 3813 3898 4969 3004
Size: 809 85 1071 3787
Fragments arranged by size:
3787 1071 809 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 2469 3004 3071 3813 3898 4969
Size: 399 605 74 535 67 742 85 1071
Cuts at: 4969 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 605 535 399 85 74
67
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2384 3107 844
Size: 1540 723 3489
Fragments arranged by size:
3489 1540 723
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2904 4247 4421 65
Size: 1183 1656 1343 174 1396
Fragments arranged by size:
1656 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 96
Size: 2322 3430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2197 2632 2077
Size: 120 435 5197
Fragments arranged by size:
5197 435 120
BstYI r'GATC_y
Cuts at: 1972 2436 3082 3449 3834 3851 5303 5389 5401
Size: 464 646 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 646 464 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 513
Size: 552 939 135 4126
Fragments arranged by size:
4126 939 552 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2176 487
Size: 683 56 950 4063
Fragments arranged by size:
4063 950 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 3268 4062 4082 2605
Size: 663 794 20 4275
Fragments arranged by size:
4275 794 663 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2277 2334
Size: 54 1425 50 275 18 357 90 57
Cuts at: 2334 2543 2604 3199 4088 5717 8
Size: 209 61 595 889 1629 43
Fragments arranged by size:
1629 1425 889 595 357 275 209 90
61 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2633 2738 3682 1353
Size: 1280 105 944 3423
Fragments arranged by size:
3423 1280 944 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3002 3002
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2311 2325 2413 2474 2853 3003
Size: 393 647 32 14 88 61 379 150
Cuts at: 3003 3815 4412 5199 5467 1239
Size: 812 597 787 268 1524
Fragments arranged by size:
1524 812 787 647 597 393 379 268
150 88 61 32 14
EcoO109I rG'GnC_Cy
Cuts at: 2449 2449
Size: 5752
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
EcoRV GAT'ATC
Cuts at: 2487 2487
Size: 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 5752
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2068 4899 1678
Size: 390 2831 2531
Fragments arranged by size:
2831 2531 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 2565 2597 2748
Size: 32 135 32 574 32 387 32 151
Cuts at: 2748 2780 4393 4425 4467 4499 1373
Size: 32 1613 32 42 32 2626
Fragments arranged by size:
2626 1613 574 387 151 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 2019 5628 2019
Size: 3609 2143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2818 4866 5050 1305
Size: 1513 2048 184 2007
Fragments arranged by size:
2048 2007 1513 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 2279 2336 10
Size: 54 1425 343 357 90 57 3426
Fragments arranged by size:
3426 1425 357 343 90 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 2366 2630 3710 4069 4228 518
Size: 648 930 270 264 1080 359 159 2042
Fragments arranged by size:
2042 1080 930 648 359 270 264 159
MspA1I CmG'CkG
Cuts at: 1606 2972 3168 3845 4993 5238 1606
Size: 1366 196 677 1148 245 2120
Fragments arranged by size:
2120 1366 1148 677 245 196
NcoI C'CATG_G
Cuts at: 513 1065 2004 2139 513
Size: 552 939 135 4126
Fragments arranged by size:
4126 939 552 135
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2557 3110 4655 1132
Size: 97 1328 553 1545 2229
Fragments arranged by size:
2229 1545 1328 553 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2916 1108
Size: 1808 3944
PfoI T'CCnGG_A
Cuts at: 2995 2995
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 2449
Size: 5752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2972 2972
Size: 5752
SacI G_AGCT'C
Cuts at: 3004 3004
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SapI GCTCTTCn'nnn_
Cuts at: 2633 2633
Size: 5752
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 3100 4235 4916 5107 826
Size: 219 2055 1135 681 191 1471
Fragments arranged by size:
2055 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 2139 2395 2943 3191 513
Size: 552 939 135 256 548 248 3074
Fragments arranged by size:
3074 939 552 548 256 248 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2065 3779 3796 3949 4134 4579 325
Size: 1255 485 1714 17 153 185 445 1498
Fragments arranged by size:
1714 1498 1255 485 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 3998 5710
Size: 274 80 33 51 1443 441 1580 1712
Cuts at: 5710 96
Size: 138
Fragments arranged by size:
1712 1580 1443 441 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 2815 2197
Size: 618 5134
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 2895 2991 3881 2895
Size: 96 890 4766
Fragments arranged by size:
4766 890 96
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI