Restriction map for
pFN21AE6350
(Circular) (Six-base) MAPSORT of: pFN21AE6350.seq Check: 6604 from: 1 to: 5758
pFN21AE6350 5758 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 3821 5205 5473 1632
Size: 941 1248 1384 268 1917
Fragments arranged by size:
1917 1384 1248 941 268
AflII C'TTAA_G
Cuts at: 820 1017 2639 820
Size: 197 1622 3939
Fragments arranged by size:
3939 1622 197
AflIII A'CryG_T
Cuts at: 1203 2248 2250 2420 4657 5634 1203
Size: 1045 2 170 2237 977 1327
Fragments arranged by size:
2237 1327 1045 977 170 2
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2563 3067 1184
Size: 1379 504 3875
Fragments arranged by size:
3875 1379 504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2127
Size: 32 5726
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2389 2412 2628 2829 3003 3067 3293 3445
Size: 896 23 216 201 174 64 226 152
Cuts at: 3445 5554 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 896 226 216 201 174 152
64 23
AseI AT'TA_AT
Cuts at: 160 1032 2901 4312 160
Size: 872 1869 1411 1606
Fragments arranged by size:
1869 1606 1411 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2076 3083 1870
Size: 73 109 24 1007 4545
Fragments arranged by size:
4545 1007 109 73 24
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 3079 4534 618
Size: 325 217 627 332 960 1455 1842
Fragments arranged by size:
1842 1455 960 627 332 325 217
BanII G_rGCy'C
Cuts at: 1391 3077 1391
Size: 1686 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 928
Size: 971 560 4227
Fragments arranged by size:
4227 971 560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4369 136
Size: 107 122 71 631 605 337 2360 1525
Fragments arranged by size:
2360 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2124 3819 4975 1790
Size: 334 1695 1156 2573
Fragments arranged by size:
2573 1695 1156 334
BmgBI CAC'GTC
Cuts at: 1318 2843 1318
Size: 1525 4233
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4418 1239
Size: 3179 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2251 3549 493
Size: 711 1047 1298 2702
Fragments arranged by size:
2702 1298 1047 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2411 1186
Size: 895 330 4533
Fragments arranged by size:
4533 895 330
BseYI C'CCAG_C
Cuts at: 2026 4961 5457 2026
Size: 2935 496 2327
Fragments arranged by size:
2935 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2337 2865 1163
Size: 573 601 528 4056
Fragments arranged by size:
4056 601 573 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsmI GAATG_Cn'
Cuts at: 2654 3269 3362 2654
Size: 615 93 5050
Fragments arranged by size:
5050 615 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 3077 3819 3904 4975 1391
Size: 399 334 953 742 85 1071 2174
Fragments arranged by size:
2174 1071 953 742 399 334 85
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3113 844
Size: 2269 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 4253 4427 65
Size: 1183 1759 1246 174 1396
Fragments arranged by size:
1759 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 4402 4589 1700
Size: 110 250 2342 187 2869
Fragments arranged by size:
2869 2342 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 2747 96
Size: 2651 3107
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2272 794
Size: 1478 4280
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2312 2312
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753
Size: 1116 367 385 17 1452 86 12 346
Cuts at: 5753 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2639 2758 487
Size: 683 56 1413 119 3487
Fragments arranged by size:
3487 1413 683 119 56
BtsI GCAGTG_nn'
Cuts at: 2349 3274 4068 4088 2349
Size: 925 794 20 4019
Fragments arranged by size:
4019 925 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723
Size: 54 1425 50 275 18 1375 889 1629
Cuts at: 5723 8
Size: 43
Fragments arranged by size:
1629 1425 1375 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3688 1353
Size: 2335 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2062 2092 4345 4719 4865 387
Size: 1064 138 473 30 2253 374 146 1280
Fragments arranged by size:
2253 1280 1064 473 374 146 138 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2573 3821 4418 5205 5473 1239
Size: 393 941 1248 597 787 268 1524
Fragments arranged by size:
1524 1248 941 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2735 2735
Size: 5758
EcoRI G'AATT_C
Cuts at: 3003 3067 3445 3003
Size: 64 378 5316
Fragments arranged by size:
5316 378 64
EcoRV GAT'ATC
Cuts at: 2534 2534
Size: 5758
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373
Size: 32 135 32 2827 32 42 32 2626
Fragments arranged by size:
2827 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2113 4872 5056 1305
Size: 808 2759 184 2007
Fragments arranged by size:
2759 2007 808 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2305 2760 3716 4075 4234 518
Size: 648 1139 455 956 359 159 2042
Fragments arranged by size:
2042 1139 956 648 455 359 159
MspA1I CmG'CkG
Cuts at: 1606 3174 3851 4999 5244 1606
Size: 1568 677 1148 245 2120
Fragments arranged by size:
2120 1568 1148 677 245
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 5758
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2424 3116 4661 1132
Size: 97 1195 692 1545 2229
Fragments arranged by size:
2229 1545 1195 692 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 2420 4657 2420
Size: 2237 3521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2921 1108
Size: 1813 3945
PfoI T'CCnGG_A
Cuts at: 2914 2914
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PmlI CAC'GTG
Cuts at: 2251 2251
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2735 2735
Size: 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 2562 3328 2562
Size: 766 4992
PstI C_TGCA'G
Cuts at: 830 2975 3110 830
Size: 2145 135 3478
Fragments arranged by size:
3478 2145 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2971 3106 4241 4922 5113 826
Size: 219 1926 135 1135 681 191 1471
Fragments arranged by size:
1926 1471 1135 681 219 191 135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 2054 3085 1872
Size: 182 1031 4545
Fragments arranged by size:
4545 1031 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3862 4763 5025 820
Size: 197 926 696 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 696 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 2566 3682 5
Size: 47 2514 1116 2081
Fragments arranged by size:
2514 2081 1116 47
StuI AGG'CCT
Cuts at: 1517 2012 1517
Size: 495 5263
StyI C'CwwG_G
Cuts at: 513 1065 2004 2020 3197 513
Size: 552 939 16 1177 3074
Fragments arranged by size:
3074 1177 939 552 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2747 4004 5716
Size: 274 80 33 51 1443 770 1257 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1443 1257 770 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 2719 3094 2719
Size: 375 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2304 2304
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 2052 3083 1870
Size: 182 1031 4545
Fragments arranged by size:
4545 1031 182
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI
NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI