Restriction map for
pFN21AE6349
(Circular) (Six-base) MAPSORT of: pFN21AE6349.seq Check: 4931 from: 1 to: 5875
pFN21AE6349 5875 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2033 2033
Size: 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 2313 3196 2313
Size: 883 4992
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2883 3938 5322 5590 1632
Size: 1251 1055 1384 268 1917
Fragments arranged by size:
1917 1384 1251 1055 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 2029 4604 2029
Size: 2575 3300
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 2935 3618 5190 2935
Size: 683 1572 3620
Fragments arranged by size:
3620 1572 683
ApaI G_GGCC'C
Cuts at: 2857 3131 2857
Size: 274 5601
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 2138 2292 2372 3055 3184 3410 3562 5671
Size: 645 154 80 683 129 226 152 2109
Cuts at: 5671 1493
Size: 1697
Fragments arranged by size:
2109 1697 683 645 226 154 152 129
80
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 3200 1870
Size: 73 1257 4545
Fragments arranged by size:
4545 1257 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2313 3196 4651 618
Size: 325 217 627 526 883 1455 1842
Fragments arranged by size:
1842 1455 883 627 526 325 217
BanII G_rGCy'C
Cuts at: 1391 2857 3131 3194 1391
Size: 1466 274 63 4072
Fragments arranged by size:
4072 1466 274 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2889 928
Size: 971 990 3914
Fragments arranged by size:
3914 990 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2754 3767 4977 2754
Size: 1013 1210 3652
Fragments arranged by size:
3652 1210 1013
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2611 2922 5870 2611
Size: 311 2948 2616
Fragments arranged by size:
2948 2616 311
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2817 2857 3131 3936 5092 1790
Size: 1027 40 274 805 1156 2573
Fragments arranged by size:
2573 1156 1027 805 274 40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2892 2945 4535 1239
Size: 812 841 53 1590 2579
Fragments arranged by size:
2579 1590 841 812 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4000 4865 5163 4000
Size: 865 298 4712
Fragments arranged by size:
4712 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3026 4538 882
Size: 617 1527 1512 2219
Fragments arranged by size:
2219 1527 1512 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5650
Fragments arranged by size:
5650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3084 1186
Size: 1898 3977
BseYI C'CCAG_C
Cuts at: 5078 5574 5078
Size: 496 5379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3936 4021 5092 3936
Size: 85 1071 4719
Fragments arranged by size:
4719 1071 85
BsmI GAATG_Cn'
Cuts at: 2778 3386 3479 2778
Size: 608 93 5174
Fragments arranged by size:
5174 608 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2817 2857 3131 3194 3936 4021 5092
Size: 399 1027 40 274 63 742 85 1071
Cuts at: 5092 1391
Size: 2174
Fragments arranged by size:
2174 1071 1027 742 399 274 85 63
40
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 2482 3764 5494 2482
Size: 1282 1730 2863
Fragments arranged by size:
2863 1730 1282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 2962 3230 844
Size: 1189 929 268 3489
Fragments arranged by size:
3489 1189 929 268
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 4519 4706 1700
Size: 110 311 2398 187 2869
Fragments arranged by size:
2869 2398 311 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3117 794
Size: 2323 3552
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2075 2436 2611 2922 3205 3572 3957 3974
Size: 103 361 175 311 283 367 385 17
Cuts at: 3974 5426 5512 5524 5870 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 361 346 311 283
175 103 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 2647 2847 513
Size: 552 939 15 628 200 3541
Fragments arranged by size:
3541 939 628 552 200 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 3391 4185 4205 3391
Size: 794 20 5061
Fragments arranged by size:
5061 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3060 3179 3060
Size: 119 5756
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2677 3322 4211
Size: 54 1425 50 275 18 847 645 889
Cuts at: 4211 5840 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 847 645 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3017 3805 1353
Size: 1664 788 3423
Fragments arranged by size:
3423 1664 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2883 2892 2945 3938 4535 5322
Size: 393 419 832 9 53 993 597 787
Cuts at: 5322 5590 1239
Size: 268 1524
Fragments arranged by size:
1524 993 832 787 597 419 393 268
53 9
EcoO109I rG'GnC_Cy
Cuts at: 2853 2854 3127 3128 2853
Size: 1 273 1 5600
Fragments arranged by size:
5600 273 1 1
EcoRI G'AATT_C
Cuts at: 2372 3184 3562 2372
Size: 812 378 4685
Fragments arranged by size:
4685 812 378
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 5022 1678
Size: 3344 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2934 2966 3063
Size: 32 135 32 443 32 887 32 97
Cuts at: 3063 3095 4516 4548 4590 4622 1373
Size: 32 1421 32 42 32 2626
Fragments arranged by size:
2626 1421 887 443 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 2317 3200 2317
Size: 883 4992
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4989 5173 1305
Size: 3684 184 2007
Fragments arranged by size:
3684 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2679 10
Size: 54 1425 343 847 3206
Fragments arranged by size:
3206 1425 847 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3833 4192 4351 518
Size: 648 2667 359 159 2042
Fragments arranged by size:
2667 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3291 3968 5116 5361 1606
Size: 1685 677 1148 245 2120
Fragments arranged by size:
2120 1685 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2019 2647 513
Size: 552 939 15 628 3741
Fragments arranged by size:
3741 939 628 552 15
NdeI CA'TA_TG
Cuts at: 387 2809 387
Size: 2422 3453
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 3004 3081 1108
Size: 1236 660 77 3902
Fragments arranged by size:
3902 1236 660 77
PfoI T'CCnGG_A
Cuts at: 2961 2961
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2853 3127 2853
Size: 274 5601
PstI C_TGCA'G
Cuts at: 830 2563 2839 3227 830
Size: 1733 276 388 3478
Fragments arranged by size:
3478 1733 388 276
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 2559 2835 3223 4358 5039 5230 826
Size: 219 1514 276 388 1135 681 191 1471
Fragments arranged by size:
1514 1471 1135 681 388 276 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3979 4880 5142 820
Size: 197 926 2036 901 262 1553
Fragments arranged by size:
2036 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 2155 3799 5
Size: 47 2103 1644 2081
Fragments arranged by size:
2103 2081 1644 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 2004 2019 2526 2647 3036 3163 3314
Size: 552 939 15 507 121 389 127 151
Cuts at: 3314 513
Size: 3074
Fragments arranged by size:
3074 939 552 507 389 151 127 121
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2992 3902 3919 4072 4257 4702 325
Size: 1255 1412 910 17 153 185 445 1498
Fragments arranged by size:
1498 1412 1255 910 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4121 5833 96
Size: 274 80 33 51 1443 2144 1712 138
Fragments arranged by size:
2144 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 5835
Size: 32 5843
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2414 2414
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 2499 2510 2535 4004 2499
Size: 11 25 1469 4370
Fragments arranged by size:
4370 1469 25 11
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI