Restriction map for
pFN21AE6346
(Circular) (Six-base) MAPSORT of: pFN21AE6346.seq Check: 5285 from: 1 to: 6001
pFN21AE6346 6001 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2051 2051
Size: 6001
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3231 3344 1937
Size: 1294 113 4594
Fragments arranged by size:
4594 1294 113
Acc65I G'GTAC_C
Cuts at: 2155 3322 2155
Size: 1167 4834
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 4064 5448 5716 1632
Size: 535 1897 1384 268 1917
Fragments arranged by size:
1917 1897 1384 535 268
AfeI AGC'GCT
Cuts at: 2447 2447
Size: 6001
AflII C'TTAA_G
Cuts at: 820 1017 2615 820
Size: 197 1598 4206
Fragments arranged by size:
4206 1598 197
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 2207 2556 4832 2207
Size: 349 2276 3376
Fragments arranged by size:
3376 2276 349
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 2639 3179 1166
Size: 1473 540 3988
Fragments arranged by size:
3988 1473 540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2780 1184
Size: 1596 4405
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 2447
Size: 32 5969
AlwNI CAG_nnn'CTG
Cuts at: 2332 2467 2644 3744 5316 2332
Size: 135 177 1100 1572 3017
Fragments arranged by size:
3017 1572 1100 177 135
ApaI G_GGCC'C
Cuts at: 2974 2974
Size: 6001
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
AvrII C'CTAG_G
Cuts at: 2053 2053
Size: 6001
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2537 2570 2537
Size: 33 5968
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2287 2545 2598 3322
Size: 325 217 627 368 132 258 53 724
Cuts at: 3322 4777 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 724 627 368 325 258 217
132 53
BanII G_rGCy'C
Cuts at: 1391 2262 2361 2787 2954 2974 3320 1391
Size: 871 99 426 167 20 346 4072
Fragments arranged by size:
4072 871 426 346 167 99 20
BbeI G_GCGC'C
Cuts at: 2291 2291
Size: 6001
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3094 928
Size: 971 1195 3835
Fragments arranged by size:
3835 1195 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4192 4226 1903
Size: 34 2255 34 3678
Fragments arranged by size:
3678 2255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4612 136
Size: 107 122 71 631 605 2940 1525
Fragments arranged by size:
2940 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 2588 3418 2588
Size: 830 5171
Bme1580I G_kGCm'C
Cuts at: 1790 2548 2974 4062 5218 1790
Size: 758 426 1088 1156 2573
Fragments arranged by size:
2573 1156 1088 758 426
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2725 3732 4690
Size: 416 53 83 399 24 1308 1007 958
Cuts at: 4690 442
Size: 1753
Fragments arranged by size:
1753 1308 1007 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2345 2999 4661 1239
Size: 983 123 654 1662 2579
Fragments arranged by size:
2579 1662 983 654 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 2303 4664 882
Size: 617 699 105 2361 2219
Fragments arranged by size:
2361 2219 699 617 105
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2288 3808 4190
Size: 53 83 186 936 477 278 1520 382
Cuts at: 4190 4825 275
Size: 635 1451
Fragments arranged by size:
1520 1451 936 635 477 382 278 186
83 53
BsaWI w'CCGG_w
Cuts at: 2086 2207 2556 4434 4832 5106 5253 5664 2086
Size: 121 349 1878 398 274 147 411 2423
Fragments arranged by size:
2423 1878 411 398 349 274 147 121
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 1178
Size: 30 165 30 1143 30 4603
Fragments arranged by size:
4603 1143 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6001
BseYI C'CCAG_C
Cuts at: 2100 2797 3190 5204 5700 2100
Size: 697 393 2014 496 2401
Fragments arranged by size:
2401 2014 697 496 393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2924 3311 1163
Size: 573 459 729 387 3853
Fragments arranged by size:
3853 729 573 459 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4062 4147 5218 4062
Size: 85 1071 4845
Fragments arranged by size:
4845 1071 85
BsmI GAATG_Cn'
Cuts at: 2134 3512 3605 2134
Size: 1378 93 4530
Fragments arranged by size:
4530 1378 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2361 2548 2787 2954 2974 3320
Size: 399 472 99 187 239 167 20 346
Cuts at: 3320 4062 4147 5218 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 346 239 187
167 99 85 20
BspEI T'CCGG_A
Cuts at: 2086 5664 2086
Size: 3578 2423
BspHI T'CATG_A
Cuts at: 3114 3890 5620 3114
Size: 776 1730 3495
Fragments arranged by size:
3495 1730 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2051 2822 3356 844
Size: 1207 771 534 3489
Fragments arranged by size:
3489 1207 771 534
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2260 4496 4670 65
Size: 1183 1012 2236 174 1396
Fragments arranged by size:
2236 1396 1183 1012 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2207 2556 4645 4832 1700
Size: 110 397 349 2089 187 2869
Fragments arranged by size:
2869 2089 397 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2598 794
Size: 1804 4197
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 3331 3698 4083 4100 5552 5638 5650 5996
Size: 1359 367 385 17 1452 86 12 346
Cuts at: 5996 1972
Size: 1977
Fragments arranged by size:
1977 1452 1359 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4510
Fragments arranged by size:
4510 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 2954 3517 4311 4331 2031
Size: 923 563 794 20 3701
Fragments arranged by size:
3701 923 794 563 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DraIII CAC_nnn'GTG
Cuts at: 2441 2441
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 3055 3163
Size: 54 1425 50 275 18 816 409 108
Cuts at: 3163 3448 4337 5966 8
Size: 285 889 1629 43
Fragments arranged by size:
1629 1425 889 816 409 285 275 108
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2348 2945 3068 3931 1353
Size: 995 597 123 863 3423
Fragments arranged by size:
3423 995 863 597 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2203 3113 4588 4962 5108 387
Size: 1064 138 614 910 1475 374 146 1280
Fragments arranged by size:
1475 1280 1064 910 614 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 2222 2345 2999 4064 4661 5448
Size: 393 535 55 123 654 1065 597 787
Cuts at: 5448 5716 1239
Size: 268 1524
Fragments arranged by size:
1524 1065 787 654 597 535 393 268
123 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 3125 2978
Size: 147 5854
EcoO109I rG'GnC_Cy
Cuts at: 2215 2329 2970 2971 2215
Size: 114 641 1 5245
Fragments arranged by size:
5245 641 114 1
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 2291 2449 5148 1678
Size: 613 158 2699 2531
Fragments arranged by size:
2699 2531 613 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4642 4674 4716 4748 1373
Size: 32 135 32 3070 32 42 32 2626
Fragments arranged by size:
3070 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2238 2687 748
Size: 1490 449 4062
Fragments arranged by size:
4062 1490 449
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2287 2287
Size: 6001
KpnI G_GTAC'C
Cuts at: 2159 3326 2159
Size: 1167 4834
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 3057 3165 10
Size: 54 1425 343 816 409 108 2846
Fragments arranged by size:
2846 1425 816 409 343 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2639 2678 3113 3179 3959 4318
Size: 648 1152 321 39 435 66 780 359
Cuts at: 4318 4477 518
Size: 159 2042
Fragments arranged by size:
2042 1152 780 648 435 359 321 159
66 39
MspA1I CmG'CkG
Cuts at: 1606 2595 2775 3417 4094 5242 5487 1606
Size: 989 180 642 677 1148 245 2120
Fragments arranged by size:
2120 1148 989 677 642 245 180
NarI GG'CG_CC
Cuts at: 2288 2288
Size: 6001
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4510
Fragments arranged by size:
4510 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 2761 3054 3359 4904 1132
Size: 97 1532 293 305 1545 2229
Fragments arranged by size:
2229 1545 1532 305 293 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2019 2644 1108
Size: 911 625 4465
Fragments arranged by size:
4465 911 625
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PspOMI G'GGCC_C
Cuts at: 2970 2970
Size: 6001
PstI C_TGCA'G
Cuts at: 830 2394 3353 830
Size: 1564 959 3478
Fragments arranged by size:
3478 1564 959
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2595 2595
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SapI GCTCTTCn'nnn_
Cuts at: 2348 3068 2348
Size: 720 5281
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 2180 4249 5961 2180
Size: 2069 1712 2220
Fragments arranged by size:
2220 2069 1712
SfcI C'TryA_G
Cuts at: 826 1045 2390 3349 4484 5165 5356 826
Size: 219 1345 959 1135 681 191 1471
Fragments arranged by size:
1471 1345 1135 959 681 219 191
SfoI GGC'GCC
Cuts at: 2289 2289
Size: 6001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2615 4105 5006 5268 820
Size: 197 926 672 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 672 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 2267 2654 2817 3131 1517
Size: 750 387 163 314 4387
Fragments arranged by size:
4387 750 387 314 163
StyI C'CwwG_G
Cuts at: 513 1065 2004 2053 2262 2649 3440 513
Size: 552 939 49 209 387 791 3074
Fragments arranged by size:
3074 939 791 552 387 209 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2563 2651 4028 4045 4198 4383 4828
Size: 1255 983 88 1377 17 153 185 445
Cuts at: 4828 325
Size: 1498
Fragments arranged by size:
1498 1377 1255 983 445 185 153 88
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 4247 5959
Size: 274 80 33 51 1443 201 2069 1712
Cuts at: 5959 96
Size: 138
Fragments arranged by size:
2069 1712 1443 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2676 2708 5961 5993 2676
Size: 32 3253 32 2684
Fragments arranged by size:
3253 2684 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI