Restriction map for
pFN21AE6345
(Circular) (Six-base) MAPSORT of: pFN21AE6345.seq Check: 1643 from: 1 to: 5812
pFN21AE6345 5812 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2274 2379 2772 3155 1937
Size: 337 105 393 383 4594
Fragments arranged by size:
4594 393 383 337 105
Acc65I G'GTAC_C
Cuts at: 2327 3133 2327
Size: 806 5006
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 3875 5259 5527 1632
Size: 551 1692 1384 268 1917
Fragments arranged by size:
1917 1692 1384 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 2277 2561 4711 5688 1203
Size: 1074 284 2150 977 1327
Fragments arranged by size:
2150 1327 1074 977 284
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 2032 2174 2231 2632 2926 3555 5127 2032
Size: 142 57 401 294 629 1572 2717
Fragments arranged by size:
2717 1572 629 401 294 142 57
ApaI G_GGCC'C
Cuts at: 2837 2837
Size: 5812
ApaLI G'TGCA_C
Cuts at: 2465 3869 5025 2465
Size: 1404 1156 3252
Fragments arranged by size:
3252 1404 1156
ApoI r'AATT_y
Cuts at: 1493 3121 3347 3499 5608 1493
Size: 1628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2730 3137 1870
Size: 73 168 619 407 4545
Fragments arranged by size:
4545 619 407 168 73
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2472 2858 3133 4588
Size: 325 217 627 540 145 386 275 1455
Cuts at: 4588 618
Size: 1842
Fragments arranged by size:
1842 1455 627 540 386 325 275 217
145
BanII G_rGCy'C
Cuts at: 1391 2106 2245 2837 3042 3131 1391
Size: 715 139 592 205 89 4072
Fragments arranged by size:
4072 715 592 205 139 89
BbeI G_GCGC'C
Cuts at: 2862 2862
Size: 5812
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 2451 928
Size: 971 272 280 4289
Fragments arranged by size:
4289 971 280 272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2666 2881 3030
Size: 107 122 71 631 605 994 215 149
Cuts at: 3030 4423 136
Size: 1393 1525
Fragments arranged by size:
1525 1393 994 631 605 215 149 122
107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 3018 3229 3018
Size: 211 5601
Bme1580I G_kGCm'C
Cuts at: 1790 2469 2569 2837 3873 5029 1790
Size: 679 100 268 1036 1156 2573
Fragments arranged by size:
2573 1156 1036 679 268 100
BmgBI CAC'GTC
Cuts at: 1318 2203 2278 2562 1318
Size: 885 75 284 4568
Fragments arranged by size:
4568 885 284 75
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 4501 442
Size: 416 53 83 399 24 2126 958 1753
Fragments arranged by size:
2126 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2666
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2537 2759 4472 1239
Size: 935 363 222 1713 2579
Fragments arranged by size:
2579 1713 935 363 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 882
Size: 617 2976 2219
Fragments arranged by size:
2976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2077 2719 3603 493
Size: 711 873 642 884 2702
Fragments arranged by size:
2702 884 873 711 642
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2859 3619 4001 4636
Size: 53 83 186 936 1326 760 382 635
Cuts at: 4636 275
Size: 1451
Fragments arranged by size:
1451 1326 936 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2360 4245 4643 4917 5064 5475 2360
Size: 1885 398 274 147 411 2697
Fragments arranged by size:
2697 1885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2061 2091 2232 2262 1178
Size: 30 165 30 658 30 141 30 4728
Fragments arranged by size:
4728 658 165 141 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 1186
Size: 985 4827
BseYI C'CCAG_C
Cuts at: 2264 5015 5511 2264
Size: 2751 496 2565
Fragments arranged by size:
2751 2565 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2001 2342 2375 1163
Size: 573 265 341 33 4600
Fragments arranged by size:
4600 573 341 265 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2469 2946 3873 3958 5029 2076
Size: 393 477 927 85 1071 2859
Fragments arranged by size:
2859 1071 927 477 393 85
BsmI GAATG_Cn'
Cuts at: 2091 3323 3416 2091
Size: 1232 93 4487
Fragments arranged by size:
4487 1232 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2106 2245 2469 2569 2837 2946
Size: 399 286 30 139 224 100 268 109
Cuts at: 2946 3042 3131 3873 3958 5029 1391
Size: 96 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 286 268 224 139
109 100 96 89 85 30
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 2607 3701 5431 2607
Size: 1094 1730 2988
Fragments arranged by size:
2988 1730 1094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3167 844
Size: 2323 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2707 2867 4307 4481 65
Size: 1183 1459 160 1440 174 1396
Fragments arranged by size:
1459 1440 1396 1183 174 160
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3049 3872 4884 3049
Size: 823 1012 3977
Fragments arranged by size:
3977 1012 823
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 2926 794
Size: 1286 846 3680
Fragments arranged by size:
3680 1286 846
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 2420 1184
Size: 1236 4576
BstXI CCAn_nnnn'nTGG
Cuts at: 2264 2392 2264
Size: 128 5684
BstYI r'GATC_y
Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807
Size: 1170 367 385 17 1452 86 12 346
Cuts at: 5807 1972
Size: 1977
Fragments arranged by size:
1977 1452 1170 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2773 2773
Size: 5812
BtgI C'CryG_G
Cuts at: 513 1065 2004 2539 2577 2979 513
Size: 552 939 535 38 402 3346
Fragments arranged by size:
3346 939 552 535 402 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5073
Fragments arranged by size:
5073 683 56
BtsI GCAGTG_nn'
Cuts at: 2469 2927 3328 4122 4142 2469
Size: 458 401 794 20 4139
Fragments arranged by size:
4139 794 458 401 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DraIII CAC_nnn'GTG
Cuts at: 2080 2080
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2235 2499
Size: 54 1425 50 275 18 177 228 264
Cuts at: 2499 2683 2764 2933 2976 2982 3259 4148 5777
Size: 184 81 169 43 6 277 889 1629
Cuts at: 5777 8
Size: 43
Fragments arranged by size:
1629 1425 889 277 275 264 228 184
177 169 81 54 50 43 43 18
6
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2657 3742 1353
Size: 1304 1085 3423
Fragments arranged by size:
3423 1304 1085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2183 2537 2759 3875 4472 5259
Size: 393 542 9 354 222 1116 597 787
Cuts at: 5259 5527 1239
Size: 268 1524
Fragments arranged by size:
1524 1116 787 597 542 393 354 268
222 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2431 2431
Size: 5812
EcoO109I rG'GnC_Cy
Cuts at: 2144 2171 2405 2494 2629 2834 2144
Size: 27 234 89 135 205 5122
Fragments arranged by size:
5122 234 205 135 89 27
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 2862 3025 4959 1678
Size: 1184 163 1934 2531
Fragments arranged by size:
2531 1934 1184 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373
Size: 32 135 32 2881 32 42 32 2626
Fragments arranged by size:
2881 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2317 3156 3402 669
Size: 988 281 379 839 246 3079
Fragments arranged by size:
3079 988 839 379 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KasI G'GCGC_C
Cuts at: 2858 2858
Size: 5812
KpnI G_GTAC'C
Cuts at: 2331 3137 2331
Size: 806 5006
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4926 5110 1305
Size: 3621 184 2007
Fragments arranged by size:
3621 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2984 10
Size: 54 1425 343 1152 2838
Fragments arranged by size:
2838 1425 1152 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3770 4129 4288 518
Size: 648 2604 359 159 2042
Fragments arranged by size:
2604 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2231 2746 3228 3905 5053 5298 1606
Size: 492 133 515 482 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 492 482 245 133
NarI GG'CG_CC
Cuts at: 2859 2859
Size: 5812
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4321
Fragments arranged by size:
4321 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 3170 4715 1132
Size: 97 1941 1545 2229
Fragments arranged by size:
2229 1941 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5812
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2032 2140 2221 3081 1108
Size: 924 108 81 860 3839
Fragments arranged by size:
3839 924 860 108 81
PfoI T'CCnGG_A
Cuts at: 2514 2514
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PmlI CAC'GTG
Cuts at: 2077 2719 2077
Size: 642 5170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3881 3913 5394 5426 3029
Size: 32 820 32 1481 32 3415
Fragments arranged by size:
3415 1481 820 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2144 2144
Size: 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PspOMI G'GGCC_C
Cuts at: 2833 2833
Size: 5812
PstI C_TGCA'G
Cuts at: 830 2097 2922 3164 830
Size: 1267 825 242 3478
Fragments arranged by size:
3478 1267 825 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
PvuII CAG'CTG
Cuts at: 2231 2231
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SanDI GG'GwC_CC
Cuts at: 2144 2144
Size: 5812
SapI GCTCTTCn'nnn_
Cuts at: 2657 2657
Size: 5812
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SexAI A'CCwGG_T
Cuts at: 2040 2040
Size: 5812
SfcI C'TryA_G
Cuts at: 826 1045 2093 2431 2797 2918 3160 4295 4976
Size: 219 1048 338 366 121 242 1135 681
Cuts at: 4976 5167 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1048 681 366 338 242 219
191 121
SfiI GGCCn_nnn'nGGCC
Cuts at: 2666 2666
Size: 5812
SfoI GGC'GCC
Cuts at: 2860 2860
Size: 5812
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 2732 3139 1872
Size: 860 407 4545
Fragments arranged by size:
4545 860 407
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 2004 3251 513
Size: 552 939 1247 3074
Fragments arranged by size:
3074 1247 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3839 3856 4009 4194 4639 325
Size: 1255 2259 17 153 185 445 1498
Fragments arranged by size:
2259 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4058 5770 96
Size: 274 80 33 51 1443 2081 1712 138
Fragments arranged by size:
2081 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2061 2093 2232 2264 2524 2556 5772 5804 2061
Size: 32 139 32 260 32 3216 32 2069
Fragments arranged by size:
3216 2069 260 139 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 2730 3137 1870
Size: 860 407 4545
Fragments arranged by size:
4545 860 407
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacI SacII SrfI SwaI XcmI