Restriction map for
pFN21AE6340
(Circular) (Six-base) MAPSORT of: pFN21AE6340.seq Check: 6570 from: 1 to: 5761
pFN21AE6340 5761 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2410 3082 2410
Size: 672 5089
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2384 2935 3019 3824 5208 5476 1632
Size: 752 551 84 805 1384 268 1917
Fragments arranged by size:
1917 1384 805 752 551 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2054 3504 5076 2054
Size: 1450 1572 2739
Fragments arranged by size:
2739 1572 1450
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 2199 2220 2472 3070 3296 3448 5557 1493
Size: 706 21 252 598 226 152 2109 1697
Fragments arranged by size:
2109 1697 706 598 252 226 152 21
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2410 3082 4537 618
Size: 325 217 627 623 672 1455 1842
Fragments arranged by size:
1842 1455 672 627 623 325 217
BanII G_rGCy'C
Cuts at: 1391 2353 3080 1391
Size: 962 727 4072
Fragments arranged by size:
4072 962 727
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 2872 1548
Size: 1324 4437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2800 5756 2800
Size: 2956 2805
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 3822 4978 1790
Size: 2032 1156 2573
Fragments arranged by size:
2573 2032 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2148 3492 4450
Size: 416 53 83 399 24 731 1344 958
Cuts at: 4450 442
Size: 1753
Fragments arranged by size:
1753 1344 958 731 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 4421 1239
Size: 1280 1902 2579
Fragments arranged by size:
2579 1902 1280
Bpu10I CC'TnA_GC
Cuts at: 2354 2354
Size: 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2463 2561 3886 4751 5049 2463
Size: 98 1325 865 298 3175
Fragments arranged by size:
3175 1325 865 298 98
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2195 2768 4424 882
Size: 617 696 573 1656 2219
Fragments arranged by size:
2219 1656 696 617 573
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3950 4585 275
Size: 53 83 186 936 2035 382 635 1451
Fragments arranged by size:
2035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 1186
Size: 1234 4527
BseYI C'CCAG_C
Cuts at: 2289 2915 4964 5460 2289
Size: 626 2049 496 2590
Fragments arranged by size:
2590 2049 626 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2851 3822 3907 4978 2851
Size: 971 85 1071 3634
Fragments arranged by size:
3634 1071 971 85
BsmI GAATG_Cn'
Cuts at: 2287 2614 3272 3365 2287
Size: 327 658 93 4683
Fragments arranged by size:
4683 658 327 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2353 2851 3080 3822 3907 4978 1391
Size: 399 563 498 229 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 498 399 229 85
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 2116 3650 5380 2116
Size: 1534 1730 2497
Fragments arranged by size:
2497 1730 1534
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 3038 3116 844
Size: 1224 970 78 3489
Fragments arranged by size:
3489 1224 970 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2420 4256 4430 65
Size: 1183 1172 1836 174 1396
Fragments arranged by size:
1836 1396 1183 1172 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2800 3091 3458 3843 3860 5312 5398 5410
Size: 828 291 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 828 385 367 346 291 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2615 2615
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 513
Size: 552 939 129 4141
Fragments arranged by size:
4141 939 552 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 2569 3065 2569
Size: 496 5265
DraIII CAC_nnn'GTG
Cuts at: 2058 2058
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2601 3208 4097
Size: 54 1425 50 275 18 771 607 889
Cuts at: 4097 5726 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 771 607 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 3691 1353
Size: 1028 1310 3423
Fragments arranged by size:
3423 1310 1028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2384 2519 2935 3019 3824 4421 5208
Size: 393 752 135 416 84 805 597 787
Cuts at: 5208 5476 1239
Size: 268 1524
Fragments arranged by size:
1524 805 787 752 597 416 393 268
135 84
EcoO109I rG'GnC_Cy
Cuts at: 2484 2484
Size: 5761
EcoRI G'AATT_C
Cuts at: 2472 3070 3448 2472
Size: 598 378 4785
Fragments arranged by size:
4785 598 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2392 2580 2392
Size: 188 5573
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 4908 1678
Size: 3230 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2722 2754 2774 2806 4402
Size: 32 135 32 1150 32 20 32 1596
Cuts at: 4402 4434 4476 4508 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1596 1150 135 42 32 32 32
32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 2414 3086 2414
Size: 672 5089
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 10
Size: 54 1425 343 771 3168
Fragments arranged by size:
3168 1425 771 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 3719 4078 4237 518
Size: 648 867 1686 359 159 2042
Fragments arranged by size:
2042 1686 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 3177 3854 5002 5247 1606
Size: 756 815 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 756 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2133 513
Size: 552 939 129 4141
Fragments arranged by size:
4141 939 552 129
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2484 2484
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2859 2938 3048 3113 830
Size: 2029 79 110 65 3478
Fragments arranged by size:
3478 2029 110 79 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2362 2362
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SapI GCTCTTCn'nnn_
Cuts at: 2381 2381
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2522 2855 2934 3044 3109 4244 4925
Size: 219 1477 333 79 110 65 1135 681
Cuts at: 4925 5116 826
Size: 191 1471
Fragments arranged by size:
1477 1471 1135 681 333 219 191 110
79 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2478 2576 3865 4766 5028 820
Size: 197 926 535 98 1289 901 262 1553
Fragments arranged by size:
1553 1289 926 901 535 262 197 98
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 3200 513
Size: 552 939 129 1067 3074
Fragments arranged by size:
3074 1067 939 552 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 2167 2303 3788 3805 3958 4143
Size: 1255 443 144 136 1485 17 153 185
Cuts at: 4143 4588 325
Size: 445 1498
Fragments arranged by size:
1498 1485 1255 445 443 185 153 144
136 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2089 4007 5719
Size: 274 80 33 51 1443 112 1918 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1918 1712 1443 274 138 112 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2818 2850 5721 5753 2818
Size: 32 2871 32 2826
Fragments arranged by size:
2871 2826 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 2063 2797 3097 2063
Size: 734 300 4727
Fragments arranged by size:
4727 734 300
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 2259 3890 2259
Size: 1631 4130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI