Restriction map for
pFN21AE6338
(Circular) (Six-base) MAPSORT of: pFN21AE6338.seq Check: 4439 from: 1 to: 5644
pFN21AE6338 5644 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2046 3707 5091 5359 1632
Size: 414 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 414 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2941 4543 5520 1203
Size: 1738 1602 977 1327
Fragments arranged by size:
1738 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 2013 4373 2013
Size: 2360 3284
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaLI G'TGCA_C
Cuts at: 2374 3701 4857 2374
Size: 1327 1156 3161
Fragments arranged by size:
3161 1327 1156
ApoI r'AATT_y
Cuts at: 1493 2645 2953 3179 3331 5440 1493
Size: 1152 308 226 152 2109 1697
Fragments arranged by size:
2109 1697 1152 308 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2700 2969 1870
Size: 73 757 269 4545
Fragments arranged by size:
4545 757 269 73
BamHI G'GATC_C
Cuts at: 2779 2974 3341 2779
Size: 195 367 5082
Fragments arranged by size:
5082 367 195
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4420 618
Size: 325 217 627 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2284 2963 1391
Size: 893 679 4072
Fragments arranged by size:
4072 893 679
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2813 928
Size: 971 914 3759
Fragments arranged by size:
3759 971 914
BbvCI CC'TCA_GC
Cuts at: 2547 2547
Size: 5644
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2532 3536 4746 2532
Size: 1004 1210 3430
Fragments arranged by size:
3430 1210 1004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2378 3705 4861 1790
Size: 588 1327 1156 2573
Fragments arranged by size:
2573 1327 1156 588
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2828 3375 4333
Size: 416 53 83 399 24 1411 547 958
Cuts at: 4333 442
Size: 1753
Fragments arranged by size:
1753 1411 958 547 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2298 2298
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 4304 1239
Size: 923 2142 2579
Fragments arranged by size:
2579 2142 923
Bpu10I CC'TnA_GC
Cuts at: 2547 2547
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2268 2408 2538 3769 4634 4932 2268
Size: 140 130 1231 865 298 2980
Fragments arranged by size:
2980 1231 865 298 140 130
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2081 4077 4475 4749 4896 5307 2081
Size: 1996 398 274 147 411 2418
Fragments arranged by size:
2418 1996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2378 3705 3790 4861 2378
Size: 1327 85 1071 3161
Fragments arranged by size:
3161 1327 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2378 2963 3705 3790 4861 1391
Size: 399 494 94 585 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 585 494 399 94 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2019 4139 4313 65
Size: 1183 771 2120 174 1396
Fragments arranged by size:
2120 1396 1183 771 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2779 2974 3341 3726 3743 5195 5281 5293
Size: 807 195 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 807 385 367 346 195 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2065 513
Size: 552 1000 4092
Fragments arranged by size:
4092 1000 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2181 2765 3160 3954 3974 2181
Size: 584 395 794 20 3851
Fragments arranged by size:
3851 794 584 395 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3091 3980 5609
Size: 54 1425 50 275 18 1261 889 1629
Cuts at: 5609 8
Size: 43
Fragments arranged by size:
1629 1425 1261 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2838 4231 4605 4751 387
Size: 1064 138 1249 1393 374 146 1280
Fragments arranged by size:
1393 1280 1249 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2162 3707 4304 5091 5359 1239
Size: 393 414 116 1545 597 787 268 1524
Fragments arranged by size:
1545 1524 787 597 414 393 268 116
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
EcoRV GAT'ATC
Cuts at: 2575 2575
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2121 4791 1678
Size: 443 2670 2531
Fragments arranged by size:
2670 2531 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2464 2496 2559
Size: 32 135 32 427 32 433 32 63
Cuts at: 2559 2591 4285 4317 4359 4391 1373
Size: 32 1694 32 42 32 2626
Fragments arranged by size:
2626 1694 433 427 135 63 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2883 748
Size: 2135 3509
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2070 2631 3602 3961 4120 518
Size: 648 904 561 971 359 159 2042
Fragments arranged by size:
2042 971 904 648 561 359 159
MspA1I CmG'CkG
Cuts at: 1606 3060 3737 4885 5130 1606
Size: 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2065 513
Size: 552 1000 4092
Fragments arranged by size:
4092 1000 552
NdeI CA'TA_TG
Cuts at: 387 2266 387
Size: 1879 3765
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2609 2945 3002 4547 1132
Size: 97 1380 336 57 1545 2229
Fragments arranged by size:
2229 1545 1380 336 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 2941 4543 2941
Size: 1602 4042
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PfoI T'CCnGG_A
Cuts at: 2396 2396
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2748 2996 830
Size: 1918 248 3478
Fragments arranged by size:
3478 1918 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2744 2992 4127 4808 4999 826
Size: 219 1699 248 1135 681 191 1471
Fragments arranged by size:
1699 1471 1135 681 248 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2702 2971 1872
Size: 830 269 4545
Fragments arranged by size:
4545 830 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2283 2387 2553 3748 4649 4911
Size: 197 926 340 104 166 1195 901 262
Cuts at: 4911 820
Size: 1553
Fragments arranged by size:
1553 1195 926 901 340 262 197 166
104
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2065 3083 513
Size: 552 1000 1018 3074
Fragments arranged by size:
3074 1018 1000 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3671 3688 3841 4026 4471 325
Size: 1255 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2524 2365
Size: 159 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2700 2969 1870
Size: 830 269 4545
Fragments arranged by size:
4545 830 269
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI