Restriction map for
pFN21AE6336
(Circular) (Six-base) MAPSORT of: pFN21AE6336.seq Check: 8093 from: 1 to: 5995
pFN21AE6336 5995 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 2650 2782 2957 3080 4058 5442 5710
Size: 995 23 132 175 123 978 1384 268
Cuts at: 5710 1632
Size: 1917
Fragments arranged by size:
1917 1384 995 978 268 175 132 123
23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 2621 2623 3239 4894 5871 1203
Size: 1418 2 616 1655 977 1327
Fragments arranged by size:
1655 1418 1327 977 616 2
AgeI A'CCGG_T
Cuts at: 2220 4826 2220
Size: 2606 3389
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2118 1184
Size: 934 5061
AlwNI CAG_nnn'CTG
Cuts at: 2568 3070 3738 5310 2568
Size: 502 668 1572 3253
Fragments arranged by size:
3253 1572 668 502
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 2789 3304 3530 3682 5791 1493
Size: 1296 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 1296 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 3320 1870
Size: 73 187 1190 4545
Fragments arranged by size:
4545 1190 187 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2610 2643 2610
Size: 33 5962
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2898 3316 4771 618
Size: 325 217 627 1111 418 1455 1842
Fragments arranged by size:
1842 1455 1111 627 418 325 217
BanII G_rGCy'C
Cuts at: 1391 2841 3314 1391
Size: 1450 473 4072
Fragments arranged by size:
4072 1450 473
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BbvCI CC'TCA_GC
Cuts at: 2720 2720
Size: 5995
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2255 2289 4186 4220 1903
Size: 34 318 34 1897 34 3678
Fragments arranged by size:
3678 1897 318 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2032 2053 3143
Size: 107 122 71 631 605 360 21 1090
Cuts at: 3143 4606 136
Size: 1463 1525
Fragments arranged by size:
1525 1463 1090 631 605 360 122 107
71 21
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 2015 2877 3412 2015
Size: 862 535 4598
Fragments arranged by size:
4598 862 535
Bme1580I G_kGCm'C
Cuts at: 1790 4056 5212 1790
Size: 2266 1156 2573
Fragments arranged by size:
2573 2266 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2588 2588
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 2924 3305 4655 1239
Size: 1394 291 381 1350 2579
Fragments arranged by size:
2579 1394 1350 381 291
Bpu10I CC'TnA_GC
Cuts at: 2720 2930 2720
Size: 210 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 4120 4985 5283 2825
Size: 1295 865 298 3537
Fragments arranged by size:
3537 1295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2141 2744 4658 882
Size: 617 642 603 1914 2219
Fragments arranged by size:
2219 1914 642 617 603
BsaAI yAC'GTr
Cuts at: 493 1204 2624 3242 3786 493
Size: 711 1420 618 544 2702
Fragments arranged by size:
2702 1420 711 618 544
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3096 3802 4184 4819
Size: 53 83 186 936 1563 706 382 635
Cuts at: 4819 275
Size: 1451
Fragments arranged by size:
1563 1451 936 706 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2220 4428 4826 5100 5247 5658 2220
Size: 2208 398 274 147 411 2557
Fragments arranged by size:
2557 2208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2813 2843 1178
Size: 30 165 30 1410 30 4330
Fragments arranged by size:
4330 1410 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2882 2921 1186
Size: 1696 39 4260
Fragments arranged by size:
4260 1696 39
BseYI C'CCAG_C
Cuts at: 2088 5198 5694 2088
Size: 3110 496 2389
Fragments arranged by size:
3110 2389 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4206 5234 1540
Size: 275 186 1444 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2841 4056 4141 5212 2139
Size: 702 1215 85 1071 2922
Fragments arranged by size:
2922 1215 1071 702 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2841 3314 4056 4141 5212 1391
Size: 399 349 702 473 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 473 399 349 85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2319 2334 3350 844
Size: 1235 240 15 1016 3489
Fragments arranged by size:
3489 1235 1016 240 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2690 3882 1394
Size: 429 867 1192 3507
Fragments arranged by size:
3507 1192 867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3132 4490 4664 65
Size: 1183 1884 1358 174 1396
Fragments arranged by size:
1884 1396 1358 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2220 2370 2973 4639 4826 1700
Size: 110 410 150 603 1666 187 2869
Fragments arranged by size:
2869 1666 603 410 187 150 110
BsrGI T'GTAC_A
Cuts at: 96 2829 96
Size: 2733 3262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2311 794
Size: 1517 4478
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstXI CCAn_nnnn'nTGG
Cuts at: 3160 3175 3160
Size: 15 5980
BstYI r'GATC_y
Cuts at: 1972 2115 2588 2911 3325 3692 4077 4094 5546
Size: 143 473 323 414 367 385 17 1452
Cuts at: 5546 5632 5644 5990 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 473 414 385 367 346 323
143 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3222 3222
Size: 5995
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3034 487
Size: 683 56 1808 3448
Fragments arranged by size:
3448 1808 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 2730 3511 4305 4325 2660
Size: 70 781 794 20 4330
Fragments arranged by size:
4330 794 781 70 20
ClaI AT'CG_AT
Cuts at: 1554 2271 1554
Size: 717 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DraIII CAC_nnn'GTG
Cuts at: 3171 3171
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2340 3291
Size: 54 1425 50 275 18 177 333 951
Cuts at: 3291 3442 4331 5960 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 951 889 333 275 177 151
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3925 1353
Size: 2572 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 4582 4956 5102 387
Size: 1064 138 519 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 519 374 146 138
EcoICRI GAG'CTC
Cuts at: 2839 2839
Size: 5995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2627 2633 2650 2782 2924 2957 3080
Size: 393 995 6 17 132 142 33 123
Cuts at: 3080 3305 4058 4655 5442 5710 1239
Size: 225 753 597 787 268 1524
Fragments arranged by size:
1524 995 787 753 597 393 268 225
142 132 123 33 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 2906 2498
Size: 408 5587
EcoO109I rG'GnC_Cy
Cuts at: 2395 2395
Size: 5995
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2878 2470
Size: 408 5587
FspI TGC'GCA
Cuts at: 2918 4501 2918
Size: 1583 4412
HaeII r_GCGC'y
Cuts at: 1678 2038 5142 1678
Size: 360 3104 2531
Fragments arranged by size:
3104 2531 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373
Size: 32 135 32 3064 32 42 32 2626
Fragments arranged by size:
3064 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2218 2614 3339 3585 669
Size: 988 281 280 396 725 246 3079
Fragments arranged by size:
3079 988 725 396 281 280 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2533 2550 5109 5293 1305
Size: 1228 17 2559 184 2007
Fragments arranged by size:
2559 2007 1228 184 17
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2342 10
Size: 54 1425 343 177 333 3663
Fragments arranged by size:
3663 1425 343 333 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 3953 4312 4471 518
Size: 648 1218 1569 359 159 2042
Fragments arranged by size:
2042 1569 1218 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2092 3283 3411 4088 5236 5481 1606
Size: 438 48 1191 128 677 1148 245 2120
Fragments arranged by size:
2120 1191 1148 677 438 245 128 48
NaeI GCC'GGC
Cuts at: 2372 2372
Size: 5995
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NgoMIV G'CCGG_C
Cuts at: 2370 2370
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 3353 4898 1132
Size: 97 2124 1545 2229
Fragments arranged by size:
2229 2124 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5995
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PmlI CAC'GTG
Cuts at: 2624 2624
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2395
Size: 5995
PshAI GACnn'nnGTC
Cuts at: 1613 2244 2667 1613
Size: 631 423 4941
Fragments arranged by size:
4941 631 423
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2097 2316 2886 3126 3347 830
Size: 1267 219 570 240 221 3478
Fragments arranged by size:
3478 1267 570 240 221 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2044 2092 3283 2044
Size: 48 1191 4756
Fragments arranged by size:
4756 1191 48
SacI G_AGCT'C
Cuts at: 2841 2841
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SbfI CC_TGCA'GG
Cuts at: 2316 3347 2316
Size: 1031 4964
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2093 2312 2882 3122 3343 4478 5159
Size: 219 1048 219 570 240 221 1135 681
Cuts at: 5159 5350 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1048 681 570 240 221 219
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 2132 3322 1872
Size: 260 1190 4545
Fragments arranged by size:
4545 1190 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2840 4099 5000 5262 820
Size: 197 926 897 1259 901 262 1553
Fragments arranged by size:
1553 1259 926 901 897 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 2453 2477 1517
Size: 936 24 5035
Fragments arranged by size:
5035 936 24
StyI C'CwwG_G
Cuts at: 513 1065 2004 2862 3434 513
Size: 552 939 858 572 3074
Fragments arranged by size:
3074 939 858 572 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2700 4022 4039 4192 4377 4822 325
Size: 1255 1120 1322 17 153 185 445 1498
Fragments arranged by size:
1498 1322 1255 1120 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 4241 5953
Size: 274 80 33 51 1443 852 1412 1712
Cuts at: 5953 96
Size: 138
Fragments arranged by size:
1712 1443 1412 852 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3155 3187 3247 3279 5955 5987 3086
Size: 32 37 32 60 32 2676 32 3094
Fragments arranged by size:
3094 2676 60 37 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 2130 3320 1870
Size: 260 1190 4545
Fragments arranged by size:
4545 1190 260
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI