Restriction map for
pFN21AE6335
(Circular) (Six-base) MAPSORT of: pFN21AE6335.seq Check: 2400 from: 1 to: 5920
pFN21AE6335 5920 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3730 278
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3263 1937
Size: 1326 4594
Acc65I G'GTAC_C
Cuts at: 3241 3241
Size: 5920
AclI AA'CG_TT
Cuts at: 1563 4047 4420 1563
Size: 2484 373 3063
Fragments arranged by size:
3063 2484 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 3983 5367 5635 1632
Size: 998 1353 1384 268 1917
Fragments arranged by size:
1917 1384 1353 998 268
AfeI AGC'GCT
Cuts at: 2129 2508 2129
Size: 379 5541
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5723
AflIII A'CryG_T
Cuts at: 1203 2658 4819 5796 1203
Size: 1455 2161 977 1327
Fragments arranged by size:
2161 1455 1327 977
AgeI A'CCGG_T
Cuts at: 4751 4751
Size: 5920
AhdI GACnn_n'nnGTC
Cuts at: 4649 4649
Size: 5920
AleI CACnn'nnGTG
Cuts at: 1166 2108 1166
Size: 942 4978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2060 1184
Size: 876 5044
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 5888
AlwNI CAG_nnn'CTG
Cuts at: 2500 2768 3663 5235 2500
Size: 268 895 1572 3185
Fragments arranged by size:
3185 1572 895 268
ApaLI G'TGCA_C
Cuts at: 3977 5133 3977
Size: 1156 4764
ApoI r'AATT_y
Cuts at: 1493 3229 3455 3607 5716 1493
Size: 1736 226 152 2109 1697
Fragments arranged by size:
2109 1736 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2192 4474 160
Size: 872 1160 2282 1606
Fragments arranged by size:
2282 1606 1160 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
AvaI C'yCGr_G
Cuts at: 1870 1943 2877 3245 1870
Size: 73 934 368 4545
Fragments arranged by size:
4545 934 368 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2459 2492 2459
Size: 33 5887
BamHI G'GATC_C
Cuts at: 3250 3617 3250
Size: 367 5553
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2665 3241 4696 618
Size: 325 217 627 285 593 576 1455 1842
Fragments arranged by size:
1842 1455 627 593 576 325 285 217
BanII G_rGCy'C
Cuts at: 1391 3239 1391
Size: 1848 4072
BbeI G_GCGC'C
Cuts at: 2076 2076
Size: 5920
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2709 3120 928
Size: 971 810 411 3728
Fragments arranged by size:
3728 971 810 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2533 2567 3082 3116 4111 4145 1903
Size: 34 596 34 515 34 995 34 3678
Fragments arranged by size:
3678 995 596 515 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3812 5022 3812
Size: 1210 4710
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5920
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2639 2756 2777
Size: 107 122 71 631 605 967 117 21
Cuts at: 2777 4531 136
Size: 1754 1525
Fragments arranged by size:
1754 1525 967 631 605 122 117 107
71 21
BglII A'GATC_T
Cuts at: 2025 3186 5915 2025
Size: 1161 2729 2030
Fragments arranged by size:
2729 2030 1161
BlpI GC'TnA_GC
Cuts at: 3337 3337
Size: 5920
Bme1580I G_kGCm'C
Cuts at: 1790 3981 5137 1790
Size: 2191 1156 2573
Fragments arranged by size:
2573 2191 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5920
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3139 3651 4609
Size: 416 53 83 399 24 1722 512 958
Cuts at: 4609 442
Size: 1753
Fragments arranged by size:
1753 1722 958 512 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3107 1056
Size: 2051 3869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 5920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2343 3143 4580 1239
Size: 866 238 800 1437 2579
Fragments arranged by size:
2579 1437 866 800 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2120 4045 4910 5208 2105
Size: 15 1925 865 298 2817
Fragments arranged by size:
2817 1925 865 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4583 882
Size: 617 3084 2219
Fragments arranged by size:
3084 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3711 493
Size: 711 2507 2702
Fragments arranged by size:
2702 2507 711
BsaBI GATnn'nnATC
Cuts at: 5914 5914
Size: 5920
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 3727 4109 4744
Size: 53 83 186 936 540 1654 382 635
Cuts at: 4744 275
Size: 1451
Fragments arranged by size:
1654 1451 936 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2460 2620 2847 2988 3001 4353 4751 5025 5172
Size: 160 227 141 13 1352 398 274 147
Cuts at: 5172 5583 2460
Size: 411 2797
Fragments arranged by size:
2797 1352 411 398 274 227 160 147
141 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2384 2414 2891 2921 1178
Size: 30 165 30 981 30 477 30 4177
Fragments arranged by size:
4177 981 477 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2102 2822 3020 1186
Size: 847 69 720 198 4086
Fragments arranged by size:
4086 847 720 198 69
BseYI C'CCAG_C
Cuts at: 2980 3076 5123 5619 2980
Size: 96 2047 496 3281
Fragments arranged by size:
3281 2047 496 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2036 3092 1163
Size: 573 297 3 1056 3991
Fragments arranged by size:
3991 1056 573 297 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3370 4131 5159 1540
Size: 275 186 1369 761 1028 2301
Fragments arranged by size:
2301 1369 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2149 2293 3981 4066 5137 2149
Size: 144 1688 85 1071 2932
Fragments arranged by size:
2932 1688 1071 144 85
BsmI GAATG_Cn'
Cuts at: 2551 2965 3431 3524 2551
Size: 414 466 93 4947
Fragments arranged by size:
4947 466 414 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2149 2293 3239 3981 4066 5137 1391
Size: 399 359 144 946 742 85 1071 2174
Fragments arranged by size:
2174 1071 946 742 399 359 144 85
BspEI T'CCGG_A
Cuts at: 2460 2620 2847 3001 5583 2460
Size: 160 227 154 2582 2797
Fragments arranged by size:
2797 2582 227 160 154
BspHI T'CATG_A
Cuts at: 3809 5539 3809
Size: 1730 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 3275 844
Size: 1559 872 3489
Fragments arranged by size:
3489 1559 872
BsrBI CCG'CTC
Cuts at: 1394 1823 3807 1394
Size: 429 1984 3507
Fragments arranged by size:
3507 1984 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2556 3098 4415 4589 65
Size: 1183 1308 542 1317 174 1396
Fragments arranged by size:
1396 1317 1308 1183 542 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4564 4751 1700
Size: 110 2754 187 2869
Fragments arranged by size:
2869 2754 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5920
BssSI C'ACGA_G
Cuts at: 3980 4992 3980
Size: 1012 4908
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5920
BstBI TT'CG_AA
Cuts at: 4724 4724
Size: 5920
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5920
BstYI r'GATC_y
Cuts at: 1972 2025 2164 2844 3186 3250 3617 4002 4019
Size: 53 139 680 342 64 367 385 17
Cuts at: 4019 5471 5557 5569 5915 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 680 385 367 346 342 139
86 64 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2670 2670
Size: 5920
BtgI C'CryG_G
Cuts at: 513 1065 2421 2676 2784 513
Size: 552 1356 255 108 3649
Fragments arranged by size:
3649 1356 552 255 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5181
Fragments arranged by size:
5181 683 56
BtsI GCAGTG_nn'
Cuts at: 3436 4230 4250 3436
Size: 794 20 5106
Fragments arranged by size:
5106 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5885
DraI TTT'AAA
Cuts at: 3224 3224
Size: 5920
DraIII CAC_nnn'GTG
Cuts at: 3147 3147
Size: 5920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2264 4927 809
Size: 507 948 2663 1802
Fragments arranged by size:
2663 1802 948 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2731 3367 4256
Size: 54 1425 50 275 18 901 636 889
Cuts at: 4256 5885 8
Size: 1629 43
Fragments arranged by size:
1629 1425 901 889 636 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3367 1537
Size: 275 1555 4090
Fragments arranged by size:
4090 1555 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 2730 3850 1353
Size: 885 492 1120 3423
Fragments arranged by size:
3423 1120 885 492
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4507 4881 5027 387
Size: 1064 138 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2343 2630 3143 3983 4580 5367
Size: 393 473 238 287 513 840 597 787
Cuts at: 5367 5635 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 597 513 473 393 287
268 238
EcoNI CCTnn'n_nnAGG
Cuts at: 2209 2209
Size: 5920
EcoO109I rG'GnC_Cy
Cuts at: 2641 3170 2641
Size: 529 5391
EcoRI G'AATT_C
Cuts at: 3229 3607 3229
Size: 378 5542
FspI TGC'GCA
Cuts at: 4426 4426
Size: 5920
HaeII r_GCGC'y
Cuts at: 1678 2076 2131 2510 2799 5067 1678
Size: 398 55 379 289 2268 2531
Fragments arranged by size:
2531 2268 398 379 289 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3142 3174 3196 3228 4561
Size: 32 135 32 1570 32 22 32 1333
Cuts at: 4561 4593 4635 4667 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1570 1333 135 42 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3264 3510 669
Size: 988 281 1326 246 3079
Fragments arranged by size:
3079 1326 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5920
HpaI GTT'AAC
Cuts at: 3510 3510
Size: 5920
KasI G'GCGC_C
Cuts at: 2072 2072
Size: 5920
KpnI G_GTAC'C
Cuts at: 3245 3245
Size: 5920
MfeI C'AATT_G
Cuts at: 3519 3519
Size: 5920
MluI A'CGCG_T
Cuts at: 5796 5796
Size: 5920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 5034 5218 1305
Size: 786 2943 184 2007
Fragments arranged by size:
2943 2007 786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2733 10
Size: 54 1425 343 901 3197
Fragments arranged by size:
3197 1425 901 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2108 2486 2609 2663 3878 4237
Size: 648 843 99 378 123 54 1215 359
Cuts at: 4237 4396 518
Size: 159 2042
Fragments arranged by size:
2042 1215 843 648 378 359 159 123
99 54
MspA1I CmG'CkG
Cuts at: 1606 2423 2650 2768 2786 3076 3336 4013 5161
Size: 817 227 118 18 290 260 677 1148
Cuts at: 5161 5406 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 817 677 290 260 245 227
118 18
NarI GG'CG_CC
Cuts at: 2073 2073
Size: 5920
NcoI C'CATG_G
Cuts at: 513 1065 2676 513
Size: 552 1611 3757
Fragments arranged by size:
3757 1611 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5920
NheI G'CTAG_C
Cuts at: 1052 3103 1052
Size: 2051 3869
NotI GC'GGCC_GC
Cuts at: 3367 3367
Size: 5920
NspI r_CATG'y
Cuts at: 1132 1229 2662 3121 3278 4823 1132
Size: 97 1433 459 157 1545 2229
Fragments arranged by size:
2229 1545 1433 459 157 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5920
PciI A'CATG_T
Cuts at: 2658 4819 2658
Size: 2161 3759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2682 1108
Size: 1574 4346
PfoI T'CCnGG_A
Cuts at: 2238 2253 3054 2238
Size: 15 801 5104
Fragments arranged by size:
5104 801 15
PmeI GTTT'AAAC
Cuts at: 3224 3224
Size: 5920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 3989 4021 5502 5534 2891
Size: 32 1066 32 1481 32 3277
Fragments arranged by size:
3277 1481 1066 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5920
PsiI TTA'TAA
Cuts at: 3490 3490
Size: 5920
PstI C_TGCA'G
Cuts at: 830 3186 3272 830
Size: 2356 86 3478
Fragments arranged by size:
3478 2356 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5920
PvuII CAG'CTG
Cuts at: 2650 2768 3076 2650
Size: 118 308 5494
Fragments arranged by size:
5494 308 118
SacII CC_GC'GG
Cuts at: 2424 2787 2424
Size: 363 5557
SalI G'TCGA_C
Cuts at: 1936 3262 1936
Size: 1326 4594
SbfI CC_TGCA'GG
Cuts at: 3272 3272
Size: 5920
ScaI AGT'ACT
Cuts at: 4168 5880 4168
Size: 1712 4208
SfcI C'TryA_G
Cuts at: 826 1045 3182 3268 4403 5084 5275 826
Size: 219 2137 86 1135 681 191 1471
Fragments arranged by size:
2137 1471 1135 681 219 191 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 2756 2756
Size: 5920
SfoI GGC'GCC
Cuts at: 2074 2074
Size: 5920
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5920
SmaI CCC'GGG
Cuts at: 1872 3247 1872
Size: 1375 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2135 4024 4925 5187 820
Size: 197 926 177 15 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 262 197 177 15
SnaBI TAC'GTA
Cuts at: 493 3711 493
Size: 3218 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5920
SphI G_CATG'C
Cuts at: 1132 3278 1132
Size: 2146 3774
SspI AAT'ATT
Cuts at: 5 52 3844 5
Size: 47 3792 2081
Fragments arranged by size:
3792 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5920
StyI C'CwwG_G
Cuts at: 513 1065 2637 2676 3359 513
Size: 552 1572 39 683 3074
Fragments arranged by size:
3074 1572 683 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3158 3947 3964 4117 4302 4747 325
Size: 1255 1578 789 17 153 185 445 1498
Fragments arranged by size:
1578 1498 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4166 5878 96
Size: 274 80 33 51 1443 2189 1712 138
Fragments arranged by size:
2189 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2710 2742 5880 5912 2710
Size: 32 3138 32 2718
Fragments arranged by size:
3138 2718 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5920
XbaI T'CTAG_A
Cuts at: 3191 3256 3191
Size: 65 5855
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2236 2236
Size: 5920
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5920
XmaI C'CCGG_G
Cuts at: 1870 3245 1870
Size: 1375 4545
XmnI GAAnn'nnTTC
Cuts at: 2027 4049 2027
Size: 2022 3898
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3728 276
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI
SwaI