Restriction map for
pFN21AE6333
(Circular) (Six-base) MAPSORT of: pFN21AE6333.seq Check: 8805 from: 1 to: 5665
pFN21AE6333 5665 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2806 2806
Size: 5665
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 3475 278
Size: 53 83 186 936 582 1357 2468
Fragments arranged by size:
2468 1357 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2894 3728 5112 5380 1632
Size: 491 771 834 1384 268 1917
Fragments arranged by size:
1917 1384 834 771 491 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 2248 4564 5541 1203
Size: 1045 2316 977 1327
Fragments arranged by size:
2316 1327 1045 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AlwNI CAG_nnn'CTG
Cuts at: 2066 2212 3408 4980 2066
Size: 146 1196 1572 2751
Fragments arranged by size:
2751 1572 1196 146
ApaLI G'TGCA_C
Cuts at: 2363 2411 3722 4878 2363
Size: 48 1311 1156 3150
Fragments arranged by size:
3150 1311 1156 48
ApoI r'AATT_y
Cuts at: 1493 2354 2915 2974 3200 3352 5461 1493
Size: 861 561 59 226 152 2109 1697
Fragments arranged by size:
2109 1697 861 561 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 2349 2990 1870
Size: 73 148 258 641 4545
Fragments arranged by size:
4545 641 258 148 73
BamHI G'GATC_C
Cuts at: 2995 3362 2995
Size: 367 5298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2834 2986 4441 618
Size: 325 217 627 1047 152 1455 1842
Fragments arranged by size:
1842 1455 1047 627 325 217 152
BanII G_rGCy'C
Cuts at: 1391 2460 2984 1391
Size: 1069 524 4072
Fragments arranged by size:
4072 1069 524
BbeI G_GCGC'C
Cuts at: 2838 2838
Size: 5665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2923 928
Size: 971 1024 3670
Fragments arranged by size:
3670 1024 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3557 4767 3557
Size: 1210 4455
BclI T'GATC_A
Cuts at: 1548 2100 1548
Size: 552 5113
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2396 4276 136
Size: 107 122 71 631 605 724 1880 1525
Fragments arranged by size:
1880 1525 724 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 2367 2415 3726 4882 1790
Size: 577 48 1311 1156 2573
Fragments arranged by size:
2573 1311 1156 577 48
BmgBI CAC'GTC
Cuts at: 1318 2251 1318
Size: 933 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 4325 1239
Size: 938 2148 2579
Fragments arranged by size:
2579 2148 938
Bpu10I CC'TnA_GC
Cuts at: 2448 2448
Size: 5665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 2555 5659 2555
Size: 3104 2561
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 2346 2835 3472
Size: 53 83 186 936 582 231 489 637
Cuts at: 3472 3854 4489 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 637 635 582 489 382 231
186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2670 2700 1178
Size: 30 165 30 1267 30 4143
Fragments arranged by size:
4143 1267 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 1186
Size: 1048 4617
BseYI C'CCAG_C
Cuts at: 2066 2168 2254 2864 4868 5364 2066
Size: 102 86 610 2004 496 2367
Fragments arranged by size:
2367 2004 610 496 102 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5092
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2367 2415 3726 3811 4882 2367
Size: 48 1311 85 1071 3150
Fragments arranged by size:
3150 1311 1071 85 48
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2415 2460 2984 3726 3811 4882
Size: 399 577 48 45 524 742 85 1071
Cuts at: 4882 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 577 524 399 85 48
45
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2670 2806 3020 844
Size: 1826 136 214 3489
Fragments arranged by size:
3489 1826 214 136
BsrBI CCG'CTC
Cuts at: 1394 1823 3552 1394
Size: 429 1729 3507
Fragments arranged by size:
3507 1729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4160 4334 65
Size: 1183 2912 174 1396
Fragments arranged by size:
2912 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 2610 96
Size: 2514 3151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2212 794
Size: 1418 4247
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstYI r'GATC_y
Cuts at: 1972 2124 2995 3362 3747 3764 5216 5302 5314
Size: 152 871 367 385 17 1452 86 12
Cuts at: 5314 5660 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 871 385 367 346 152 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2182 2418 2487 513
Size: 552 939 178 236 69 3691
Fragments arranged by size:
3691 939 552 236 178 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2167 2680 2935 3181 3975 3995 2067
Size: 100 513 255 246 794 20 3737
Fragments arranged by size:
3737 794 513 255 246 100 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DraIII CAC_nnn'GTG
Cuts at: 2419 2488 2419
Size: 69 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2773 3112
Size: 54 1425 50 275 18 567 376 339
Cuts at: 3112 4001 5630 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 567 376 339 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 3595 1353
Size: 1373 869 3423
Fragments arranged by size:
3423 1373 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2177 2894 3728 4325 5112 5380
Size: 393 491 54 717 834 597 787 268
Cuts at: 5380 1239
Size: 1524
Fragments arranged by size:
1524 834 787 717 597 491 393 268
54
EcoO109I rG'GnC_Cy
Cuts at: 2513 2794 2854 2513
Size: 281 60 5324
Fragments arranged by size:
5324 281 60
EcoRI G'AATT_C
Cuts at: 2354 2915 2974 3352 2354
Size: 561 59 378 4667
Fragments arranged by size:
4667 561 378 59
FspI TGC'GCA
Cuts at: 2432 4171 2432
Size: 1739 3926
HaeII r_GCGC'y
Cuts at: 1678 2099 2152 2838 4812 1678
Size: 421 53 686 1974 2531
Fragments arranged by size:
2531 1974 686 421 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2670 2702 4306
Size: 32 135 32 566 32 500 32 1604
Cuts at: 4306 4338 4380 4412 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1604 566 500 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KasI G'GCGC_C
Cuts at: 2834 2834
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2252 2722 4779 4963 1305
Size: 947 470 2057 184 2007
Fragments arranged by size:
2057 2007 947 470 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 2775 10
Size: 54 1425 343 567 376 2900
Fragments arranged by size:
2900 1425 567 376 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3623 3982 4141 518
Size: 648 2457 359 159 2042
Fragments arranged by size:
2457 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2713 3081 3758 4906 5151 1606
Size: 1107 368 677 1148 245 2120
Fragments arranged by size:
2120 1148 1107 677 368 245
NarI GG'CG_CC
Cuts at: 2835 2835
Size: 5665
NcoI C'CATG_G
Cuts at: 513 1065 2004 2182 513
Size: 552 939 178 3996
Fragments arranged by size:
3996 939 552 178
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2759 3023 4568 1132
Size: 97 1530 264 1545 2229
Fragments arranged by size:
2229 1545 1530 264 97
PasI CC'CwG_GG
Cuts at: 1348 2839 1348
Size: 1491 4174
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 4285
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2217 2580 3017 830
Size: 1387 363 437 3478
Fragments arranged by size:
3478 1387 437 363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2213 2576 2590 2735 3013 4148 4829
Size: 219 1168 363 14 145 278 1135 681
Cuts at: 4829 5020 826
Size: 191 1471
Fragments arranged by size:
1471 1168 1135 681 363 278 219 191
145 14
SfoI GGC'GCC
Cuts at: 2836 2836
Size: 5665
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2351 2992 1872
Size: 479 641 4545
Fragments arranged by size:
4545 641 479
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 2759 3023 1132
Size: 1627 264 3774
Fragments arranged by size:
3774 1627 264
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2198 1517
Size: 681 4984
StyI C'CwwG_G
Cuts at: 513 1065 2004 2182 2460 3104 513
Size: 552 939 178 278 644 3074
Fragments arranged by size:
3074 939 644 552 278 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3692 3709 3862 4047 4492 325
Size: 1255 2112 17 153 185 445 1498
Fragments arranged by size:
2112 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2610 3911 5623
Size: 274 80 33 51 1443 633 1301 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1712 1443 1301 633 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 5633
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2053
Size: 5665
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2349 2990 1870
Size: 479 641 4545
Fragments arranged by size:
4545 641 479
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 3473 276
Size: 53 83 186 936 582 1357 2468
Fragments arranged by size:
2468 1357 936 582 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI