Restriction map for
pFN21AE6332
(Circular) (Six-base) MAPSORT of: pFN21AE6332.seq Check: 6706 from: 1 to: 5950
pFN21AE6332 5950 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2247 3293 1937
Size: 310 1046 4594
Fragments arranged by size:
4594 1046 310
Acc65I G'GTAC_C
Cuts at: 2959 3271 2959
Size: 312 5638
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 4013 5397 5665 1632
Size: 650 1731 1384 268 1917
Fragments arranged by size:
1917 1731 1384 650 268
AfeI AGC'GCT
Cuts at: 2348 3026 2348
Size: 678 5272
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 2047 2439 3693 5265 2047
Size: 392 1254 1572 2732
Fragments arranged by size:
2732 1572 1254 392
ApaI G_GGCC'C
Cuts at: 2360 2360
Size: 5950
ApaLI G'TGCA_C
Cuts at: 2878 4007 5163 2878
Size: 1129 1156 3665
Fragments arranged by size:
3665 1156 1129
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2595 4504 160
Size: 872 1563 1909 1606
Fragments arranged by size:
1909 1606 1563 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2514 2588 3275 1870
Size: 73 571 74 687 4545
Fragments arranged by size:
4545 687 571 74 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2609 2695 2759 2773 2893
Size: 325 217 627 822 86 64 14 120
Cuts at: 2893 2959 3007 3271 4726 618
Size: 66 48 264 1455 1842
Fragments arranged by size:
1842 1455 822 627 325 264 217 120
86 66 64 48 14
BanII G_rGCy'C
Cuts at: 1391 2360 2800 3269 1391
Size: 969 440 469 4072
Fragments arranged by size:
4072 969 469 440
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2270 928
Size: 971 371 4608
Fragments arranged by size:
4608 971 371
BbvCI CC'TCA_GC
Cuts at: 2639 2639
Size: 5950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4141 4175 1903
Size: 34 50 34 2120 34 3678
Fragments arranged by size:
3678 2120 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2579 4561 136
Size: 107 122 71 631 605 907 1982 1525
Fragments arranged by size:
1982 1525 907 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 2429 3367 2429
Size: 938 5012
Bme1580I G_kGCm'C
Cuts at: 1790 2360 2483 2508 2612 2700 2882 4011 5167
Size: 570 123 25 104 88 182 1129 1156
Cuts at: 5167 1790
Size: 2573
Fragments arranged by size:
2573 1156 1129 570 182 123 104 88
25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2232 2573 1056
Size: 1176 341 4433
Fragments arranged by size:
4433 1176 341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2363 4610 1239
Size: 993 131 2247 2579
Fragments arranged by size:
2579 2247 993 131
Bpu10I CC'TnA_GC
Cuts at: 2639 2639
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4613 882
Size: 617 3114 2219
Fragments arranged by size:
3114 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2956 2992 3741 493
Size: 711 1752 36 749 2702
Fragments arranged by size:
2702 1752 749 711 36
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 3757 4139 4774
Size: 53 83 186 936 684 1540 382 635
Cuts at: 4774 275
Size: 1451
Fragments arranged by size:
1540 1451 936 684 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2025 2105 4383 4781 5055 5202 5613 2025
Size: 80 2278 398 274 147 411 2362
Fragments arranged by size:
2362 2278 411 398 274 147 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2991 3021 1178
Size: 30 165 30 1588 30 4107
Fragments arranged by size:
4107 1588 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2408 1186
Size: 952 270 4728
Fragments arranged by size:
4728 952 270
BseYI C'CCAG_C
Cuts at: 2349 2583 2605 2699 2913 5153 5649 2349
Size: 234 22 94 214 2240 496 2650
Fragments arranged by size:
2650 2240 496 234 214 94 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2309 2852 1163
Size: 573 360 213 543 4261
Fragments arranged by size:
4261 573 543 360 213
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2882 4011 4096 5167 2882
Size: 1129 85 1071 3665
Fragments arranged by size:
3665 1129 1071 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2360 2483 2508 2612 2700 2800 2882
Size: 399 570 123 25 104 88 100 82
Cuts at: 2882 3269 4011 4096 5167 1391
Size: 387 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 399 387 123 104
100 88 85 82 25
BspEI T'CCGG_A
Cuts at: 2105 5613 2105
Size: 3508 2442
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2643 2931 3305 844
Size: 1484 315 288 374 3489
Fragments arranged by size:
3489 1484 374 315 288
BsrBI CCG'CTC
Cuts at: 1394 1823 3837 1394
Size: 429 2014 3507
Fragments arranged by size:
3507 2014 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4594 4781 1700
Size: 110 2784 187 2869
Fragments arranged by size:
2869 2784 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 2452 1925
Size: 527 5423
BssSI C'ACGA_G
Cuts at: 2474 2652 4010 5022 2474
Size: 178 1358 1012 3402
Fragments arranged by size:
3402 1358 1012 178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2439 2886 794
Size: 1645 447 3858
Fragments arranged by size:
3858 1645 447
BstBI TT'CG_AA
Cuts at: 2784 4754 2784
Size: 1970 3980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2416
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 3186 3280 3647 4032 4049 5501 5587 5599
Size: 1214 94 367 385 17 1452 86 12
Cuts at: 5599 5945 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1214 385 367 346 94 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2162 2169 2271 2409 513
Size: 552 1097 7 102 138 4054
Fragments arranged by size:
4054 1097 552 138 102 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2087 487
Size: 683 56 861 4350
Fragments arranged by size:
4350 861 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 3466 4260 4280 2437
Size: 1029 794 20 4107
Fragments arranged by size:
4107 1029 794 20
ClaI AT'CG_AT
Cuts at: 1554 2682 1554
Size: 1128 4822
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DraIII CAC_nnn'GTG
Cuts at: 2476 2476
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2295 2382
Size: 54 1425 50 275 18 222 243 87
Cuts at: 2382 2406 3397 4286 5915 8
Size: 24 991 889 1629 43
Fragments arranged by size:
1629 1425 991 889 275 243 222 87
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2788 2925 3880 1353
Size: 1435 137 955 3423
Fragments arranged by size:
3423 1435 955 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2282 2363 4013 4610 5397 5665
Size: 393 600 50 81 1650 597 787 268
Cuts at: 5665 1239
Size: 1524
Fragments arranged by size:
1650 1524 787 600 597 393 268 81
50
EcoO109I rG'GnC_Cy
Cuts at: 2254 2356 2971 3097 2254
Size: 102 615 126 5107
Fragments arranged by size:
5107 615 126 102
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2350 3028 5097 1678
Size: 672 678 2069 2531
Fragments arranged by size:
2531 2069 678 672
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3178 3210 4591 4623 4665
Size: 32 135 32 1606 32 1381 32 42
Cuts at: 4665 4697 1373
Size: 32 2626
Fragments arranged by size:
2626 1606 1381 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2920 3294 3540 669
Size: 988 281 982 374 246 3079
Fragments arranged by size:
3079 988 982 374 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 2963 3275 2963
Size: 312 5638
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 2408 10
Size: 54 1425 343 465 111 3552
Fragments arranged by size:
3552 1425 465 343 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2066 2810 3908 4267 4426 518
Size: 648 843 57 744 1098 359 159 2042
Fragments arranged by size:
2042 1098 843 744 648 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 2044 2083 2362 2404 2913 3366 4043 5191
Size: 438 39 279 42 509 453 677 1148
Cuts at: 5191 5436 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 509 453 438 279 245
42 39
NcoI C'CATG_G
Cuts at: 513 1065 2271 513
Size: 552 1206 4192
Fragments arranged by size:
4192 1206 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 2228 2569 1052
Size: 1176 341 4433
Fragments arranged by size:
4433 1176 341
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2452 2809 3077 3308 4853 1132
Size: 97 1223 357 268 231 1545 2229
Fragments arranged by size:
2229 1545 1223 357 268 231 97
PasI CC'CwG_GG
Cuts at: 1348 2976 1348
Size: 1628 4322
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 2220 2220
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2971 3097 2971
Size: 126 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2356 2356
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2082 3302 830
Size: 1252 1220 3478
Fragments arranged by size:
3478 1252 1220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2044 2083 2404 2044
Size: 39 321 5590
Fragments arranged by size:
5590 321 39
RsrII CG'GwC_CG
Cuts at: 2166 2166
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SapI GCTCTTCn'nnn_
Cuts at: 2788 2788
Size: 5950
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SexAI A'CCwGG_T
Cuts at: 2175 2841 2175
Size: 666 5284
SfcI C'TryA_G
Cuts at: 826 1045 2078 2248 3298 4433 5114 5305 826
Size: 219 1033 170 1050 1135 681 191 1471
Fragments arranged by size:
1471 1135 1050 1033 681 219 191 170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 2516 2590 3277 1872
Size: 644 74 687 4545
Fragments arranged by size:
4545 687 644 74
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 2809 3308 1132
Size: 1677 499 3774
Fragments arranged by size:
3774 1677 499
SspI AAT'ATT
Cuts at: 5 52 2138 3874 5
Size: 47 2086 1736 2081
Fragments arranged by size:
2086 2081 1736 47
StuI AGG'CCT
Cuts at: 1517 2721 3062 1517
Size: 1204 341 4405
Fragments arranged by size:
4405 1204 341
StyI C'CwwG_G
Cuts at: 513 1065 2271 2388 2963 3389 513
Size: 552 1206 117 575 426 3074
Fragments arranged by size:
3074 1206 575 552 426 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2911 3977 3994 4147 4332 4777 325
Size: 1255 1331 1066 17 153 185 445 1498
Fragments arranged by size:
1498 1331 1255 1066 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2991 3023 5910 5942 2991
Size: 32 2887 32 2999
Fragments arranged by size:
2999 2887 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2177 1316
Size: 861 5089
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2671 2671
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 2514 2588 3275 1870
Size: 644 74 687 4545
Fragments arranged by size:
4545 687 644 74
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI SacI SacII SanDI SfiI
SfoI SrfI SwaI