Restriction map for
pFN21AE6330
(Circular) (Six-base) MAPSORT of: pFN21AE6330.seq Check: 8328 from: 1 to: 5875
pFN21AE6330 5875 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 3218 1937
Size: 370 911 4594
Fragments arranged by size:
4594 911 370
Acc65I G'GTAC_C
Cuts at: 2771 3196 2771
Size: 425 5450
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3938 5322 5590 1632
Size: 2306 1384 268 1917
Fragments arranged by size:
2306 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 2118 4706 2118
Size: 2588 3287
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 2738 3028 3618 5190 2738
Size: 290 590 1572 3423
Fragments arranged by size:
3423 1572 590 290
ApaI G_GGCC'C
Cuts at: 2025 2092 3064 2025
Size: 67 972 4836
Fragments arranged by size:
4836 972 67
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 3200 1870
Size: 73 74 1183 4545
Fragments arranged by size:
4545 1183 74 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2763 2796 2763
Size: 33 5842
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2206 2771 3142 3196 4651
Size: 325 217 627 419 565 371 54 1455
Cuts at: 4651 618
Size: 1842
Fragments arranged by size:
1842 1455 627 565 419 371 325 217
54
BanII G_rGCy'C
Cuts at: 1391 2025 2092 2906 2959 3064 3194 1391
Size: 634 67 814 53 105 130 4072
Fragments arranged by size:
4072 814 634 130 105 67 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2643 928
Size: 971 279 465 4160
Fragments arranged by size:
4160 971 465 279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2308 3767 4977 2308
Size: 1459 1210 3206
Fragments arranged by size:
3206 1459 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2071 2092 2211 2496 2699 3064 3936
Size: 235 46 21 119 285 203 365 872
Cuts at: 3936 5092 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 872 365 285 235 203 119
46 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2795 3003 3606
Size: 416 53 83 399 24 1378 208 603
Cuts at: 3606 4564 442
Size: 958 1753
Fragments arranged by size:
1753 1378 958 603 416 399 208 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2313 2336 2363 2445 2468 2682 2760 2778
Size: 1074 23 27 82 23 214 78 18
Cuts at: 2778 2858 3001 4535 1239
Size: 80 143 1534 2579
Fragments arranged by size:
2579 1534 1074 214 143 82 80 78
27 23 23 18
Bpu10I CC'TnA_GC
Cuts at: 2519 2519
Size: 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3133 4000 4865 5163 3133
Size: 867 865 298 3845
Fragments arranged by size:
3845 867 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 4538 882
Size: 617 855 2184 2219
Fragments arranged by size:
2219 2184 855 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2118 4308 4706 4980 5127 5538 2118
Size: 2190 398 274 147 411 2455
Fragments arranged by size:
2455 2190 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2130 2160 2366 2396 2655
Size: 30 165 30 727 30 206 30 259
Cuts at: 2655 2685 2733 2751 2763 2781 2900 2930 2943
Size: 30 48 18 12 18 119 30 13
Cuts at: 2943 2973 1178
Size: 30 4080
Fragments arranged by size:
4080 727 259 206 165 119 48 30
30 30 30 30 30 30 18 18
13 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2457 2855 1186
Size: 1271 398 4206
Fragments arranged by size:
4206 1271 398
BseYI C'CCAG_C
Cuts at: 2091 2193 2798 5078 5574 2091
Size: 102 605 2280 496 2392
Fragments arranged by size:
2392 2280 605 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 1163
Size: 573 351 4951
Fragments arranged by size:
4951 573 351
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2732 2906 3936 4021 5092 2732
Size: 174 1030 85 1071 3515
Fragments arranged by size:
3515 1071 1030 174 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
BsmBI CGTCTCn'nnnn_
Cuts at: 2486 2486
Size: 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2071 2092 2211 2496 2699 2732
Size: 399 235 46 21 119 285 203 33
Cuts at: 2732 2906 2959 3064 3194 3936 4021 5092 1391
Size: 174 53 105 130 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 285 235 203 174
130 119 105 85 53 46 33 21
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3230 844
Size: 2386 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2871 4370 4544 65
Size: 1183 1623 1499 174 1396
Fragments arranged by size:
1623 1499 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2118 4519 4706 1700
Size: 110 308 2401 187 2869
Fragments arranged by size:
2869 2401 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 2952 3935 4947 2952
Size: 983 1012 3880
Fragments arranged by size:
3880 1012 983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2753 794
Size: 1959 3916
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 2029 1184
Size: 845 5030
BstYI r'GATC_y
Cuts at: 1972 3205 3572 3957 3974 5426 5512 5524 5870
Size: 1233 367 385 17 1452 86 12 346
Cuts at: 5870 1972
Size: 1977
Fragments arranged by size:
1977 1452 1233 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2198 2620 2655 2971 513
Size: 552 1133 422 35 316 3417
Fragments arranged by size:
3417 1133 552 422 316 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2697 2754 3391 4185 4205 2697
Size: 57 637 794 20 4367
Fragments arranged by size:
4367 794 637 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2543 2578 529
Size: 35 1979 35 3826
Fragments arranged by size:
3826 1979 35 35
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2963 3322 4211
Size: 54 1425 50 275 18 1133 359 889
Cuts at: 4211 5840 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1133 889 359 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3805 1353
Size: 2452 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2904 2904
Size: 5875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2313 2336 2363 2445 2468 2682 2760
Size: 393 681 23 27 82 23 214 78
Cuts at: 2760 2778 2858 3001 3938 4535 5322 5590 1239
Size: 18 80 143 937 597 787 268 1524
Fragments arranged by size:
1524 937 787 681 597 393 268 214
143 82 80 78 27 23 23 18
EcoO109I rG'GnC_Cy
Cuts at: 2021 2022 2735 2833 2928 3025 3060 2021
Size: 1 713 98 95 97 35 4836
Fragments arranged by size:
4836 713 98 97 95 35 1
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
EcoRV GAT'ATC
Cuts at: 2857 2857
Size: 5875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2168 2168
Size: 5875
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 5022 1678
Size: 3344 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2655 2687 2900 2932 4516
Size: 32 135 32 1083 32 213 32 1584
Cuts at: 4516 4548 4590 4622 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1584 1083 213 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 2775 3200 2775
Size: 425 5450
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2761 4989 5173 1305
Size: 1456 2228 184 2007
Fragments arranged by size:
2228 2007 1456 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4053
Fragments arranged by size:
4053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3833 4192 4351 518
Size: 648 2667 359 159 2042
Fragments arranged by size:
2667 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2379 2549 3291 3968 5116 5361 1606
Size: 489 284 170 742 677 1148 245 2120
Fragments arranged by size:
2120 1148 742 677 489 284 245 170
NcoI C'CATG_G
Cuts at: 513 1065 2198 2655 2971 513
Size: 552 1133 457 316 3417
Fragments arranged by size:
3417 1133 552 457 316
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2972 1108
Size: 1864 4011
PfoI T'CCnGG_A
Cuts at: 2321 2352 2321
Size: 31 5844
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2928 2928
Size: 5875
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2021 2088 3060 2021
Size: 67 972 4836
Fragments arranged by size:
4836 972 67
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2433 2465 2433
Size: 32 5843
PstI C_TGCA'G
Cuts at: 830 2749 3227 830
Size: 1919 478 3478
Fragments arranged by size:
3478 1919 478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2095 2379 2095
Size: 284 5591
SacI G_AGCT'C
Cuts at: 2906 2906
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SanDI GG'GwC_CC
Cuts at: 2928 2928
Size: 5875
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 2374 2745 3223 4358 5039 5230 826
Size: 219 1329 371 478 1135 681 191 1471
Fragments arranged by size:
1471 1329 1135 681 478 371 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 2019 3202 1872
Size: 147 1183 4545
Fragments arranged by size:
4545 1183 147
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3112 3979 4880 5142 820
Size: 197 926 1169 867 901 262 1553
Fragments arranged by size:
1553 1169 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 2986 1517
Size: 1469 4406
StyI C'CwwG_G
Cuts at: 513 1065 2198 2301 2534 2655 2971 3314 513
Size: 552 1133 103 233 121 316 343 3074
Fragments arranged by size:
3074 1133 552 343 316 233 121 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3113 3902 3919 4072 4257 4702 325
Size: 1255 1533 789 17 153 185 445 1498
Fragments arranged by size:
1533 1498 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2449 4121 5833
Size: 274 80 33 51 1443 472 1672 1712
Cuts at: 5833 96
Size: 138
Fragments arranged by size:
1712 1672 1443 472 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2130 2162 2733 2751 2765 2783 2943 2975 5835
Size: 32 571 18 14 18 160 32 2860
Cuts at: 5835 5867 2130
Size: 32 2138
Fragments arranged by size:
2860 2138 571 160 32 32 32 18
18 14
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3158 3158
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 2017 3200 1870
Size: 147 1183 4545
Fragments arranged by size:
4545 1183 147
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI