Restriction map for
pFN21AE6320
(Circular) (Six-base) MAPSORT of: pFN21AE6320.seq Check: 984 from: 1 to: 5908
pFN21AE6320 5908 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2543 2957 3093 3971 5355 5623 1632
Size: 911 414 136 878 1384 268 1917
Fragments arranged by size:
1917 1384 911 878 414 268 136
AfeI AGC'GCT
Cuts at: 2043 2366 2043
Size: 323 5585
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 3033 4807 5784 1203
Size: 1830 1774 977 1327
Fragments arranged by size:
1830 1774 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AlwNI CAG_nnn'CTG
Cuts at: 2380 3007 3651 5223 2380
Size: 627 644 1572 3065
Fragments arranged by size:
3065 1572 644 627
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 2879 3217 3443 3595 5704 1493
Size: 1386 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 338 226 152
AscI GG'CGCG_CC
Cuts at: 2077 2077
Size: 5908
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2285 2315 3233 1870
Size: 73 192 150 30 918 4545
Fragments arranged by size:
4545 918 192 150 73 30
BamHI G'GATC_C
Cuts at: 3238 3605 3238
Size: 367 5541
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2016 2582 3229 4684 618
Size: 325 217 627 229 566 647 1455 1842
Fragments arranged by size:
1842 1455 647 627 566 325 229 217
BanII G_rGCy'C
Cuts at: 1391 2292 2445 3227 1391
Size: 901 153 782 4072
Fragments arranged by size:
4072 901 782 153
BbeI G_GCGC'C
Cuts at: 2020 2020
Size: 5908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4937
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 2977 1548
Size: 1429 4479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4519 136
Size: 107 122 71 631 605 2847 1525
Fragments arranged by size:
2847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 3969 5125 1790
Size: 2179 1156 2573
Fragments arranged by size:
2573 2179 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 2846 3639
Size: 416 53 83 399 24 774 655 793
Cuts at: 3639 4597 442
Size: 958 1753
Fragments arranged by size:
1753 958 793 774 655 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2042 1056
Size: 986 4922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2689 3089 3215 4568 1239
Size: 986 464 400 126 1353 2579
Fragments arranged by size:
2579 1353 986 464 400 126
Bpu10I CC'TnA_GC
Cuts at: 3129 3129
Size: 5908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 4033 4898 5196 2345
Size: 1688 865 298 3057
Fragments arranged by size:
3057 1688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3194 4571 882
Size: 617 1695 1377 2219
Fragments arranged by size:
2219 1695 1377 617
BsaAI yAC'GTr
Cuts at: 493 1204 3036 3699 493
Size: 711 1832 663 2702
Fragments arranged by size:
2702 1832 711 663
BsaBI GATnn'nnATC
Cuts at: 5902 5902
Size: 5908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2017 3715 4097 4732
Size: 53 83 186 936 484 1698 382 635
Cuts at: 4732 275
Size: 1451
Fragments arranged by size:
1698 1451 936 635 484 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2869 4341 4739 5013 5160 5571 2869
Size: 1472 398 274 147 411 3206
Fragments arranged by size:
3206 1472 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3150 3180 1178
Size: 30 165 30 1747 30 3906
Fragments arranged by size:
3906 1747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3212 1186
Size: 2026 3882
BseYI C'CCAG_C
Cuts at: 2400 2405 5111 5607 2400
Size: 5 2706 496 2701
Fragments arranged by size:
2706 2701 496 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 2976 1163
Size: 573 471 769 4095
Fragments arranged by size:
4095 769 573 471
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3358 4119 5147 1540
Size: 275 186 1357 761 1028 2301
Fragments arranged by size:
2301 1357 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 3057 3969 4054 5125 2181
Size: 876 912 85 1071 2964
Fragments arranged by size:
2964 1071 912 876 85
BsmI GAATG_Cn'
Cuts at: 2825 2947 3419 3512 2825
Size: 122 472 93 5221
Fragments arranged by size:
5221 472 122 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2292 2445 3057 3227 3969 4054
Size: 399 391 111 153 612 170 742 85
Cuts at: 4054 5125 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 391 170 153
111 85
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3263 844
Size: 2419 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2134 3795 1394
Size: 429 311 1661 3507
Fragments arranged by size:
3507 1661 429 311
BsrDI GCAATG_nn'
Cuts at: 65 1248 4403 4577 65
Size: 1183 3155 174 1396
Fragments arranged by size:
3155 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2297 4552 4739 1700
Size: 110 338 149 2255 187 2869
Fragments arranged by size:
2869 2255 338 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 2061 2068 2077 2349 1925
Size: 136 7 9 272 5484
Fragments arranged by size:
5484 272 136 9 7
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2230 2380 794
Size: 1436 150 4322
Fragments arranged by size:
4322 1436 150
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 3238 3605 3990 4007 5459 5545 5557 5903
Size: 1266 367 385 17 1452 86 12 346
Cuts at: 5903 1972
Size: 1977
Fragments arranged by size:
1977 1452 1266 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2114 513
Size: 552 939 24 86 4307
Fragments arranged by size:
4307 939 552 86 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2427 2482 487
Size: 683 56 1201 55 3913
Fragments arranged by size:
3913 1201 683 56 55
BtsI GCAGTG_nn'
Cuts at: 2353 2649 3424 4218 4238 2353
Size: 296 775 794 20 4023
Fragments arranged by size:
4023 794 775 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873
Size: 54 1425 50 275 18 1525 889 1629
Cuts at: 5873 8
Size: 43
Fragments arranged by size:
1629 1525 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2543 2832 3838 1353
Size: 980 210 289 1006 3423
Fragments arranged by size:
3423 1006 980 289 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2381 2438 4495 4869 5015 387
Size: 1064 138 792 57 2057 374 146 1280
Fragments arranged by size:
2057 1280 1064 792 374 146 138 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2543 2689 2957 3089 3093 3215
Size: 393 593 318 146 268 132 4 122
Cuts at: 3215 3971 4568 5355 5623 1239
Size: 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 597 593 393 318 268
268 146 132 122 4
EcoO109I rG'GnC_Cy
Cuts at: 2335 2377 3040 2335
Size: 42 663 5203
Fragments arranged by size:
5203 663 42
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2020 2036 2045 2274 2368 2437 5055 1678
Size: 342 16 9 229 94 69 2618 2531
Fragments arranged by size:
2618 2531 342 229 94 69 16 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4549 4581 4623 4655 1373
Size: 32 135 32 2977 32 42 32 2626
Fragments arranged by size:
2977 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3252 3498 669
Size: 988 281 1314 246 3079
Fragments arranged by size:
3079 1314 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KasI G'GCGC_C
Cuts at: 2016 2016
Size: 5908
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5022 5206 1305
Size: 3717 184 2007
Fragments arranged by size:
3717 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2532 2687 3866 4225 4384 518
Size: 648 1366 155 1179 359 159 2042
Fragments arranged by size:
2042 1366 1179 648 359 159 155
MspA1I CmG'CkG
Cuts at: 1606 2030 2172 2504 3324 4001 5149 5394 1606
Size: 424 142 332 820 677 1148 245 2120
Fragments arranged by size:
2120 1148 820 677 424 332 245 142
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 5908
NarI GG'CG_CC
Cuts at: 2017 2017
Size: 5908
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4417
Fragments arranged by size:
4417 939 552
NdeI CA'TA_TG
Cuts at: 387 2620 387
Size: 2233 3675
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 2038 1052
Size: 986 4922
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 3266 4811 1132
Size: 97 2037 1545 2229
Fragments arranged by size:
2229 2037 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5908
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PmlI CAC'GTG
Cuts at: 3036 3036
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 2568 2922 3478 2568
Size: 354 556 4998
Fragments arranged by size:
4998 556 354
PspXI vC'TCGA_Gb
Cuts at: 2135 2135
Size: 5908
PstI C_TGCA'G
Cuts at: 830 2270 2460 3260 830
Size: 1440 190 800 3478
Fragments arranged by size:
3478 1440 800 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 5908
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SexAI A'CCwGG_T
Cuts at: 3006 3006
Size: 5908
SfcI C'TryA_G
Cuts at: 826 1045 2095 2266 2456 3256 4391 5072 5263
Size: 219 1050 171 190 800 1135 681 191
Cuts at: 5263 826
Size: 1471
Fragments arranged by size:
1471 1135 1050 800 681 219 191 190
171
SfoI GGC'GCC
Cuts at: 2018 2018
Size: 5908
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 3235 1872
Size: 1363 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 2360 4012 4913 5175 820
Size: 197 926 192 225 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 262 225 197 192
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 2249 1517
Size: 732 5176
StyI C'CwwG_G
Cuts at: 513 1065 2004 3347 513
Size: 552 939 1343 3074
Fragments arranged by size:
3074 1343 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3935 3952 4105 4290 4735 325
Size: 1255 2355 17 153 185 445 1498
Fragments arranged by size:
2355 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 4154 5866
Size: 274 80 33 51 1443 189 1988 1712
Cuts at: 5866 96
Size: 138
Fragments arranged by size:
1988 1712 1443 274 189 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3150 3182 5868 5900 3150
Size: 32 2686 32 3158
Fragments arranged by size:
3158 2686 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2990 1316
Size: 1674 4234
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2686
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 2135 1943
Size: 192 5716
XmaI C'CCGG_G
Cuts at: 1870 3233 1870
Size: 1363 4545
XmnI GAAnn'nnTTC
Cuts at: 3078 4037 3078
Size: 959 4949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PstI PvuI SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PpuMI PspOMI PsrI PvuII RsrII SacI SanDI SapI
SfiI SrfI SwaI