Restriction map for
pFN21AE6317
(Circular) (Six-base) MAPSORT of: pFN21AE6317.seq Check: 2466 from: 1 to: 5644
pFN21AE6317 5644 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2607 2987 1937
Size: 670 380 4594
Fragments arranged by size:
4594 670 380
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 2669 2883 3707 5091 5359 1632
Size: 408 629 214 824 1384 268 1917
Fragments arranged by size:
1917 1384 824 629 408 268 214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2895 4543 5520 1203
Size: 1692 1648 977 1327
Fragments arranged by size:
1692 1648 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2687 1184
Size: 1503 4141
AlwNI CAG_nnn'CTG
Cuts at: 2018 2136 2930 3387 4959 2018
Size: 118 794 457 1572 2703
Fragments arranged by size:
2703 1572 794 457 118
ApaI G_GGCC'C
Cuts at: 2568 2568
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2953 3179 3331 5440 1493
Size: 1460 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2561 2969 1870
Size: 73 618 408 4545
Fragments arranged by size:
4545 618 408 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2408 2831 2965 4420 618
Size: 325 217 627 621 423 134 1455 1842
Fragments arranged by size:
1842 1455 627 621 423 325 217 134
BanII G_rGCy'C
Cuts at: 1391 2568 2744 2963 1391
Size: 1177 176 219 4072
Fragments arranged by size:
4072 1177 219 176
BbeI G_GCGC'C
Cuts at: 2412 2412
Size: 5644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2374 2872 928
Size: 971 129 346 498 3700
Fragments arranged by size:
3700 971 498 346 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2568 2724 3705 4861 1790
Size: 778 156 981 1156 2573
Fragments arranged by size:
2573 1156 981 778 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2560 2720 4304 1239
Size: 1321 160 1584 2579
Fragments arranged by size:
2579 1584 1321 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2801 3769 4634 4932 2801
Size: 968 865 298 3513
Fragments arranged by size:
3513 968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2908 4307 882
Size: 617 1409 1399 2219
Fragments arranged by size:
2219 1409 1399 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2409 3451 3833 4468
Size: 53 83 186 936 876 1042 382 635
Cuts at: 4468 275
Size: 1451
Fragments arranged by size:
1451 1042 936 876 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2430 2752 3094 3855 4883 1540
Size: 275 186 429 322 342 761 1028 2301
Fragments arranged by size:
2301 1028 761 429 342 322 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2535 2586 3705 3790 4861 2196
Size: 339 51 1119 85 1071 2979
Fragments arranged by size:
2979 1119 1071 339 85 51
BsmI GAATG_Cn'
Cuts at: 2073 2683 3155 3248 2073
Size: 610 472 93 4469
Fragments arranged by size:
4469 610 472 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2689 2689
Size: 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2535 2568 2586 2724 2744 2963
Size: 399 406 339 33 18 138 20 219
Cuts at: 2963 3705 3790 4861 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 339 219 138
85 33 20 18
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2250 2787 2999 844
Size: 1277 129 537 212 3489
Fragments arranged by size:
3489 1277 537 212 129
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2453 4288 4475 1700
Size: 110 643 1835 187 2869
Fragments arranged by size:
2869 1835 643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 2698 1925
Size: 773 4871
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2930 794
Size: 2136 3508
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639
Size: 1002 367 385 17 1452 86 12 346
Cuts at: 5639 1972
Size: 1977
Fragments arranged by size:
1977 1452 1002 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2179 2272 2400 2919 513
Size: 552 939 175 93 128 519 3238
Fragments arranged by size:
3238 939 552 519 175 128 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2398 487
Size: 683 56 1172 3733
Fragments arranged by size:
3733 1172 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2269 2427
Size: 54 1425 50 275 18 370 69 158
Cuts at: 2427 3091 3980 5609 8
Size: 664 889 1629 43
Fragments arranged by size:
1629 1425 889 664 370 275 158 69
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2427 3091 1537
Size: 275 615 664 4090
Fragments arranged by size:
4090 664 615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2205 3574 1353
Size: 776 76 1369 3423
Fragments arranged by size:
3423 1369 776 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2074 4231 4605 4751 387
Size: 1064 138 485 2157 374 146 1280
Fragments arranged by size:
2157 1280 1064 485 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2560 2669 2720 2883 3707 4304
Size: 393 408 520 109 51 163 824 597
Cuts at: 4304 5091 5359 1239
Size: 787 268 1524
Fragments arranged by size:
1524 824 787 597 520 408 393 268
163 109 51
EcoO109I rG'GnC_Cy
Cuts at: 2015 2086 2161 2515 2772 2015
Size: 71 75 354 257 4887
Fragments arranged by size:
4887 354 257 75 71
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2412 4791 1678
Size: 734 2379 2531
Fragments arranged by size:
2531 2379 734
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2397 2429 4285
Size: 32 135 32 534 32 259 32 1856
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1856 534 259 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2608 2988 3234 669
Size: 988 281 670 380 246 3079
Fragments arranged by size:
3079 988 670 380 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KasI G'GCGC_C
Cuts at: 2408 2408
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2692 4758 4942 1305
Size: 1387 2066 184 2007
Fragments arranged by size:
2066 2007 1387 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2271 10
Size: 54 1425 343 439 3383
Fragments arranged by size:
3383 1425 439 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2262 3602 3961 4120 518
Size: 648 1005 91 1340 359 159 2042
Fragments arranged by size:
2042 1340 1005 648 359 159 91
MspA1I CmG'CkG
Cuts at: 1606 3060 3737 4885 5130 1606
Size: 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 677 245
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 5644
NarI GG'CG_CC
Cuts at: 2409 2409
Size: 5644
NcoI C'CATG_G
Cuts at: 513 1065 2004 2179 2400 513
Size: 552 939 175 221 3757
Fragments arranged by size:
3757 939 552 221 175
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2038 2345 2899 3002 4547 1132
Size: 97 809 307 554 103 1545 2229
Fragments arranged by size:
2229 1545 809 554 307 103 97
PasI CC'CwG_GG
Cuts at: 1348 2369 1348
Size: 1021 4623
PciI A'CATG_T
Cuts at: 2895 4543 2895
Size: 1648 3996
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 1108
Size: 1293 4351
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2015 2086 2515 2015
Size: 71 429 5144
Fragments arranged by size:
5144 429 71
PshAI GACnn'nnGTC
Cuts at: 1613 2603 1613
Size: 990 4654
PsiI TTA'TAA
Cuts at: 2616 3214 2616
Size: 598 5046
PspOMI G'GGCC_C
Cuts at: 2564 2564
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2132 2996 830
Size: 1302 864 3478
Fragments arranged by size:
3478 1302 864
PvuI CG_AT'CG
Cuts at: 2001 2752 2001
Size: 751 4893
SalI G'TCGA_C
Cuts at: 1936 2606 2986 1936
Size: 670 380 4594
Fragments arranged by size:
4594 670 380
SapI GCTCTTCn'nnn_
Cuts at: 2205 2205
Size: 5644
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SexAI A'CCwGG_T
Cuts at: 2097 2097
Size: 5644
SfcI C'TryA_G
Cuts at: 826 1045 2128 2992 4127 4808 4999 826
Size: 219 1083 864 1135 681 191 1471
Fragments arranged by size:
1471 1135 1083 864 681 219 191
SfoI GGC'GCC
Cuts at: 2410 2410
Size: 5644
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2816 3748 4649 4911 820
Size: 197 926 873 932 901 262 1553
Fragments arranged by size:
1553 932 926 901 873 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 2038 3002 1132
Size: 906 964 3774
Fragments arranged by size:
3774 964 906
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2179 2400 3083 513
Size: 552 939 153 22 221 683 3074
Fragments arranged by size:
3074 939 683 552 221 153 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 3671 3688 3841 4026 4471 325
Size: 1255 590 1501 17 153 185 445 1498
Fragments arranged by size:
1501 1498 1255 590 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3890 5602 96
Size: 274 80 33 51 1443 1913 1712 138
Fragments arranged by size:
1913 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2264 2264
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SfiI SrfI SwaI