Restriction map for
pFN21AE6313
(Circular) (Six-base) MAPSORT of: pFN21AE6313.seq Check: 2375 from: 1 to: 5950
pFN21AE6313 5950 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 3293 1937
Size: 334 1022 4594
Fragments arranged by size:
4594 1022 334
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3161 3227 4013 5397 5665 1632
Size: 1529 66 786 1384 268 1917
Fragments arranged by size:
1917 1529 1384 786 268 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 2385 4849 5826 1203
Size: 1182 2464 977 1327
Fragments arranged by size:
2464 1327 1182 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 2144 2435 3259 3485 3637 5746 1493
Size: 651 291 824 226 152 2109 1697
Fragments arranged by size:
2109 1697 824 651 291 226 152
AscI GG'CGCG_CC
Cuts at: 2036 2036
Size: 5950
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2175 2889 3275 1870
Size: 73 232 714 386 4545
Fragments arranged by size:
4545 714 386 232 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2307 2340 2307
Size: 33 5917
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3271 4726 618
Size: 325 217 627 1484 1455 1842
Fragments arranged by size:
1842 1484 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3269 1391
Size: 1878 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2757 928
Size: 971 858 4121
Fragments arranged by size:
4121 971 858
BbvCI CC'TCA_GC
Cuts at: 2684 2684
Size: 5950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2738 2772 4141 4175 1903
Size: 34 50 34 717 34 1369 34 3678
Fragments arranged by size:
3678 1369 717 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3213 3842 5052 3213
Size: 629 1210 4111
Fragments arranged by size:
4111 1210 629
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BfrBI ATG'CAT
Cuts at: 2655 2655
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2055 2643 4561
Size: 107 122 71 631 605 383 588 1918
Cuts at: 4561 136
Size: 1525
Fragments arranged by size:
1918 1525 631 605 588 383 122 107
71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 4011 5167 1790
Size: 2221 1156 2573
Fragments arranged by size:
2573 2221 1156
BmgBI CAC'GTC
Cuts at: 1318 2309 1318
Size: 991 4959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2219 3681 4639
Size: 416 53 83 399 24 802 1462 958
Cuts at: 4639 442
Size: 1753
Fragments arranged by size:
1753 1462 958 802 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2915
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3160 4610 1239
Size: 1921 1450 2579
Fragments arranged by size:
2579 1921 1450
Bpu10I CC'TnA_GC
Cuts at: 2684 2684
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 2886 4613 882
Size: 617 567 820 1727 2219
Fragments arranged by size:
2219 1727 820 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 3166 3741 493
Size: 711 1962 575 2702
Fragments arranged by size:
2702 1962 711 575
BsaBI GATnn'nnATC
Cuts at: 3224 5944 3224
Size: 2720 3230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2088 2907 4383 4781 5055 5202 5613 2088
Size: 819 1476 398 274 147 411 2425
Fragments arranged by size:
2425 1476 819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5725
Fragments arranged by size:
5725 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3044 1186
Size: 1858 4092
BseYI C'CCAG_C
Cuts at: 2250 2724 5153 5649 2250
Size: 474 2429 496 2551
Fragments arranged by size:
2551 2429 496 474
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 1163
Size: 573 599 4778
Fragments arranged by size:
4778 599 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3059 4011 4096 5167 3059
Size: 952 85 1071 3842
Fragments arranged by size:
3842 1071 952 85
BsmI GAATG_Cn'
Cuts at: 3092 3461 3554 3092
Size: 369 93 5488
Fragments arranged by size:
5488 369 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3059 3269 4011 4096 5167 1391
Size: 399 1269 210 742 85 1071 2174
Fragments arranged by size:
2174 1269 1071 742 399 210 85
BspEI T'CCGG_A
Cuts at: 2907 5613 2907
Size: 2706 3244
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 2449 3837 1394
Size: 429 193 433 1388 3507
Fragments arranged by size:
3507 1388 433 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 2805 3013 3211 4445 4619 65
Size: 1183 1557 208 198 1234 174 1396
Fragments arranged by size:
1557 1396 1234 1183 208 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2022 2081 4594 4781 1700
Size: 110 212 59 2513 187 2869
Fragments arranged by size:
2869 2513 212 187 110 59
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 2036 1925
Size: 111 5839
BssSI C'ACGA_G
Cuts at: 2733 4010 5022 2733
Size: 1277 1012 3661
Fragments arranged by size:
3661 1277 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2652 3166 794
Size: 1858 514 3578
Fragments arranged by size:
3578 1858 514
BstBI TT'CG_AA
Cuts at: 2748 4754 2748
Size: 2006 3944
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2118 2457 3060 513
Size: 552 1053 339 603 3403
Fragments arranged by size:
3403 1053 603 552 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 3021 3051 3466 4260 4280 3021
Size: 30 415 794 20 4691
Fragments arranged by size:
4691 794 415 30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2964 2999 529
Size: 35 2400 35 3480
Fragments arranged by size:
3480 2400 35 35
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2759 3179 4957 809
Size: 507 1443 420 1778 1802
Fragments arranged by size:
1802 1778 1443 507 420
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2769 2931
Size: 54 1425 50 275 18 253 686 162
Cuts at: 2931 2985 3063 3397 4286 5915 8
Size: 54 78 334 889 1629 43
Fragments arranged by size:
1629 1425 889 686 334 275 253 162
78 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2523 3880 1353
Size: 774 396 1357 3423
Fragments arranged by size:
3423 1357 774 396
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3160 3161 3227 4013 4610 5397 5665
Size: 393 1528 1 66 786 597 787 268
Cuts at: 5665 1239
Size: 1524
Fragments arranged by size:
1528 1524 787 786 597 393 268 66
1
EcoNI CCTnn'n_nnAGG
Cuts at: 3242 3242
Size: 5950
EcoO109I rG'GnC_Cy
Cuts at: 2068 2636 2673 2068
Size: 568 37 5345
Fragments arranged by size:
5345 568 37
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 5097 1678
Size: 3419 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 2727 2759 2820
Size: 32 135 32 926 32 197 32 61
Cuts at: 2820 2852 4591 4623 4665 4697 1373
Size: 32 1739 32 42 32 2626
Fragments arranged by size:
2626 1739 926 197 135 61 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2771 2933 3065 10
Size: 54 1425 343 939 162 132 2895
Fragments arranged by size:
2895 1425 939 343 162 132 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2606 2810 2901 3908 4267 4426 518
Size: 648 1440 204 91 1007 359 159 2042
Fragments arranged by size:
2042 1440 1007 648 359 204 159 91
MspA1I CmG'CkG
Cuts at: 1606 3366 4043 5191 5436 1606
Size: 1760 677 1148 245 2120
Fragments arranged by size:
2120 1760 1148 677 245
NaeI GCC'GGC
Cuts at: 2024 2024
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 2118 2457 3060 513
Size: 552 1053 339 603 3403
Fragments arranged by size:
3403 1053 603 552 339
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NgoMIV G'CCGG_C
Cuts at: 2022 2022
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NsiI A_TGCA'T
Cuts at: 2657 2657
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2389 3308 4853 1132
Size: 97 1160 919 1545 2229
Fragments arranged by size:
2229 1545 1160 919 97
PasI CC'CwG_GG
Cuts at: 1348 2857 1348
Size: 1509 4441
PciI A'CATG_T
Cuts at: 2385 4849 2385
Size: 2464 3486
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2498 1108
Size: 902 488 4560
Fragments arranged by size:
4560 902 488
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2673 2068
Size: 605 5345
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PstI C_TGCA'G
Cuts at: 830 3027 3302 830
Size: 2197 275 3478
Fragments arranged by size:
3478 2197 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SanDI GG'GwC_CC
Cuts at: 2673 2673
Size: 5950
SapI GCTCTTCn'nnn_
Cuts at: 2127 2127
Size: 5950
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 2945 4198 5910 2945
Size: 1253 1712 2985
Fragments arranged by size:
2985 1712 1253
SfcI C'TryA_G
Cuts at: 826 1045 2343 3023 3078 3298 4433 5114 5305
Size: 219 1298 680 55 220 1135 681 191
Cuts at: 5305 826
Size: 1471
Fragments arranged by size:
1471 1298 1135 681 680 220 219 191
55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 2842 3874 5
Size: 47 2790 1032 2081
Fragments arranged by size:
2790 2081 1032 47
StuI AGG'CCT
Cuts at: 1517 2573 3110 1517
Size: 1056 537 4357
Fragments arranged by size:
4357 1056 537
StyI C'CwwG_G
Cuts at: 513 1065 2118 2337 2457 2568 2640 3060 3389
Size: 552 1053 219 120 111 72 420 329
Cuts at: 3389 513
Size: 3074
Fragments arranged by size:
3074 1053 552 420 329 219 120 111
72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3977 3994 4147 4332 4777 325
Size: 1255 2397 17 153 185 445 1498
Fragments arranged by size:
2397 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2943 4196 5908
Size: 274 80 33 51 1443 966 1253 1712
Cuts at: 5908 96
Size: 138
Fragments arranged by size:
1712 1443 1253 966 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3193 3230 3286 3193
Size: 37 56 5857
Fragments arranged by size:
5857 56 37
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BbeI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI XcmI