Restriction map for
pFN21AE6309
(Circular) (Six-base) MAPSORT of: pFN21AE6309.seq Check: 1515 from: 1 to: 5821
pFN21AE6309 5821 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3006 3884 5268 5536 1632
Size: 1374 878 1384 268 1917
Fragments arranged by size:
1917 1384 1374 878 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 2090 4652 2090
Size: 2562 3259
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 2277 3031 3564 5136 2277
Size: 754 533 1572 2962
Fragments arranged by size:
2962 1572 754 533
ApaLI G'TGCA_C
Cuts at: 3080 3878 5034 3080
Size: 798 1156 3867
Fragments arranged by size:
3867 1156 798
ApoI r'AATT_y
Cuts at: 1493 2349 2852 3130 3356 3508 5617 1493
Size: 856 503 278 226 152 2109 1697
Fragments arranged by size:
2109 1697 856 503 278 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2295 4375 160
Size: 872 1263 2080 1606
Fragments arranged by size:
2080 1606 1263 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 3045 3146 1870
Size: 73 94 1008 101 4545
Fragments arranged by size:
4545 1008 101 94 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3142 4597 618
Size: 325 217 627 1355 1455 1842
Fragments arranged by size:
1842 1455 1355 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2335 3099 3140 1391
Size: 654 290 764 41 4072
Fragments arranged by size:
4072 764 654 290 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2316 5816 2316
Size: 3500 2321
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 3084 3882 5038 1790
Size: 1294 798 1156 2573
Fragments arranged by size:
2573 1294 1156 798
BmgBI CAC'GTC
Cuts at: 1318 2344 3091 1318
Size: 1026 747 4048
Fragments arranged by size:
4048 1026 747
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2838 3552 4510
Size: 416 53 83 399 24 1421 714 958
Cuts at: 4510 442
Size: 1753
Fragments arranged by size:
1753 1421 958 714 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 4481 1239
Size: 917 2325 2579
Fragments arranged by size:
2579 2325 917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2951 3946 4811 5109 2951
Size: 995 865 298 3663
Fragments arranged by size:
3663 995 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2504 2930 4484 882
Size: 617 1005 426 1554 2219
Fragments arranged by size:
2219 1554 1005 617 426
BsaAI yAC'GTr
Cuts at: 493 1204 2953 3612 493
Size: 711 1749 659 2702
Fragments arranged by size:
2702 1749 711 659
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2090 4254 4652 4926 5073 5484 2090
Size: 2164 398 274 147 411 2427
Fragments arranged by size:
2427 2164 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 1178
Size: 30 165 30 1362 30 4204
Fragments arranged by size:
4204 1362 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2661 1186
Size: 1475 4346
BseYI C'CCAG_C
Cuts at: 2030 5024 5520 2030
Size: 2994 496 2331
Fragments arranged by size:
2994 2331 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 2995 1163
Size: 573 774 485 3989
Fragments arranged by size:
3989 774 573 485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3084 3099 3882 3967 5038 3084
Size: 15 783 85 1071 3867
Fragments arranged by size:
3867 1071 783 85 15
BsmI GAATG_Cn'
Cuts at: 2616 3332 3425 2616
Size: 716 93 5012
Fragments arranged by size:
5012 716 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2335 3084 3099 3140 3882 3967
Size: 399 255 290 749 15 41 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 749 742 399 290 255 85
41 15
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 2629 3099 3710 5440 2629
Size: 470 611 1730 3010
Fragments arranged by size:
3010 1730 611 470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2285 3176 844
Size: 1441 891 3489
Fragments arranged by size:
3489 1441 891
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2090 2676 2939 2976 4465 4652
Size: 110 207 73 586 263 37 1489 187
Cuts at: 4652 1700
Size: 2869
Fragments arranged by size:
2869 1489 586 263 207 187 110 73
37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 2532 1184
Size: 1348 4473
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2077
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2316 2962 3151 3518 3903 3920 5372 5458
Size: 344 646 189 367 385 17 1452 86
Cuts at: 5458 5470 5816 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 646 385 367 346 344 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2155 513
Size: 552 939 151 4179
Fragments arranged by size:
4179 939 552 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2373 2613 3337 4131 4151 2373
Size: 240 724 794 20 4043
Fragments arranged by size:
4043 794 724 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2970 3005 529
Size: 35 2406 35 3345
Fragments arranged by size:
3345 2406 35 35
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2978 3268 4157
Size: 54 1425 50 275 18 1148 290 889
Cuts at: 4157 5786 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1148 889 290 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2627 2638 3751 1353
Size: 1274 11 1113 3423
Fragments arranged by size:
3423 1274 1113 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3097 3097
Size: 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 3006 3884 4481 5268 5536 1239
Size: 393 524 850 878 597 787 268 1524
Fragments arranged by size:
1524 878 850 787 597 524 393 268
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2037 2084 4968 1678
Size: 359 47 2884 2531
Fragments arranged by size:
2884 2531 359 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2947 2979 4462 4494 4536
Size: 32 135 32 1375 32 1483 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 1483 1375 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2386 3165 3411 669
Size: 988 281 448 779 246 3079
Fragments arranged by size:
3079 988 779 448 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4935 5119 1305
Size: 3630 184 2007
Fragments arranged by size:
3630 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3779 4138 4297 518
Size: 648 2613 359 159 2042
Fragments arranged by size:
2613 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3237 3914 5062 5307 1606
Size: 1631 677 1148 245 2120
Fragments arranged by size:
2120 1631 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2155 513
Size: 552 939 151 4179
Fragments arranged by size:
4179 939 552 151
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2895 1108
Size: 1787 4034
PfoI T'CCnGG_A
Cuts at: 2847 2847
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PmlI CAC'GTG
Cuts at: 2953 2953
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3003 3173 830
Size: 2173 170 3478
Fragments arranged by size:
3478 2173 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SacI G_AGCT'C
Cuts at: 3099 3099
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 2972 4069 5781 2972
Size: 1097 1712 3012
Fragments arranged by size:
3012 1712 1097
SfcI C'TryA_G
Cuts at: 826 1045 2999 3169 4304 4985 5176 826
Size: 219 1954 170 1135 681 191 1471
Fragments arranged by size:
1954 1471 1135 681 219 191 170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 2039 3047 3148 1872
Size: 167 1008 101 4545
Fragments arranged by size:
4545 1008 167 101
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2966 3925 4826 5088 820
Size: 197 926 1023 959 901 262 1553
Fragments arranged by size:
1553 1023 959 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2004 2155 2326 2698 2760 3260 513
Size: 552 939 151 171 372 62 500 3074
Fragments arranged by size:
3074 939 552 500 372 171 151 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2370 2970 4067
Size: 274 80 33 51 1443 393 600 1097
Cuts at: 4067 5779 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1097 600 393 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2765 2797 5781 5813 2765
Size: 32 2984 32 2773
Fragments arranged by size:
2984 2773 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 2410 3157 2410
Size: 747 5074
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2767 2767
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 2037 3045 3146 1870
Size: 167 1008 101 4545
Fragments arranged by size:
4545 1008 167 101
XmnI GAAnn'nnTTC
Cuts at: 2867 3950 2867
Size: 1083 4738
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI